Article ; Online: Retrieval and purification of human β cells from stem cell-derived islet engrafted mice.
STAR protocols
2021 Volume 2, Issue 3, Page(s) 100675
Abstract: Stem cell-derived beta cells (SC-β-cells) engrafted into mice serve as a pre-clinical model of diabetes. It is helpful to recover viable β cells following transplantation to perform tests on the graft. We developed a protocol to retrieve and purify a ... ...
| Abstract | Stem cell-derived beta cells (SC-β-cells) engrafted into mice serve as a pre-clinical model of diabetes. It is helpful to recover viable β cells following transplantation to perform tests on the graft. We developed a protocol to retrieve and purify a sufficient number of live β cells from mice following long-term human SC-β-cell engraftment. The protocol enables examination of SC-β-cells undergoing developmental and metabolic changes |
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| MeSH term(s) | Animals ; Cell Separation/methods ; Heterografts ; Humans ; Insulin-Secreting Cells/cytology ; Insulin-Secreting Cells/transplantation ; Islets of Langerhans Transplantation/methods ; Mice ; Stem Cells/cytology |
| Language | English |
| Publishing date | 2021-07-15 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ISSN | 2666-1667 |
| ISSN (online) | 2666-1667 |
| DOI | 10.1016/j.xpro.2021.100675 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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