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Article ; Online: Molecular detection of human Plasmodium species using a multiplex real time PCR.

Lazrek, Yassamine / Florimond, Célia / Volney, Béatrice / Discours, Manon / Mosnier, Emilie / Houzé, Sandrine / Pelleau, Stéphane / Musset, Lise

Scientific reports

2023 Volume 13, Issue 1, Page(s) 11388

Abstract: Molecular detection methods have revealed higher sensitivity and specificity than conventional microscopy or rapid diagnostic tests for malaria diagnosis. In this study, we implemented, evaluated and validated according to the ISO 15,189 requirements, a ... ...

Abstract	Molecular detection methods have revealed higher sensitivity and specificity than conventional microscopy or rapid diagnostic tests for malaria diagnosis. In this study, we implemented, evaluated and validated according to the ISO 15,189 requirements, a multiplex real-time PCR assay to detect and identify the five human malaria parasites. DNA samples were extracted from whole blood or dried blood spots drawn from patients. Based on the External Quality Assessment (whole blood), this method shows 100% sensitivity and specificity. This PCR detected P. vivax up to 0.25 p/µl, P. falciparum and P. knowlesi up to 0.5 p/µl, P. ovale up to 1 p/µl and P. malariae up to 5 p/µl of blood. From blood spots (extraction from four punches), it detected P. vivax at 5 p/µl, P. falciparum, P. ovale and P. knowlesi at 20 p/µl and P. malariae at 125 p/µl. In conclusion, this quantitative PCR shows excellent performance, is easy to use and DNA saver. It is especially useful to actively screen large population groups and identify the five human malaria parasites in a context of low malaria transmission.
MeSH term(s)	Humans ; Real-Time Polymerase Chain Reaction/methods ; Plasmodium/genetics ; Malaria/parasitology ; Malaria, Vivax/parasitology ; Malaria, Falciparum ; Sensitivity and Specificity ; Plasmodium vivax/genetics ; Plasmodium falciparum/genetics
Language	English
Publishing date	2023-07-14
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-38621-9
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Article: Seroepidemiology of the Seasonal Human Coronaviruses NL63, 229E, OC43 and HKU1 in France.

De Thoisy, Alix / Woudenberg, Tom / Pelleau, Stéphane / Donnadieu, Françoise / Garcia, Laura / Pinaud, Laurie / Tondeur, Laura / Meola, Annalisa / Arowas, Laurence / Clement, Nathalie / Backovic, Marija / Ungeheuer, Marie-Noëlle / Fontanet, Arnaud / White, Michael

Open forum infectious diseases

2023 Volume 10, Issue 7, Page(s) ofad340

Abstract: Background: The seasonal human coronaviruses (HCoV) NL63, 229E, OC43, and HKU1 are globally endemic, yet the majority of HCoV infections remain undiagnosed.: Methods: In a cross-sectional study, 2389 serum samples were collected from children and ... ...

Abstract	Background: The seasonal human coronaviruses (HCoV) NL63, 229E, OC43, and HKU1 are globally endemic, yet the majority of HCoV infections remain undiagnosed. Methods: In a cross-sectional study, 2389 serum samples were collected from children and adults in France in 2020. In a longitudinal cohort study, 2520 samples were collected from 898 French individuals followed up between 2020 and 2021. Antibodies to HCoVs were measured using a bead-based multiplex assay. Results: The rate of waning of anti-HCoV spike immunoglobulin G antibodies was estimated as 0.22-0.47 year Conclusions: The high force of infection in children indicates that HCoVs may be responsible for a substantial proportion of fever episodes experienced by children.
Language	English
Publishing date	2023-07-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	2757767-3
ISSN	2328-8957
ISSN	2328-8957
DOI	10.1093/ofid/ofad340
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Article ; Online: Onset and Relapse of Juvenile Dermatomyositis Following Asymptomatic SARS-CoV-2 Infection.

Rodero, Mathieu Paul / Pelleau, Stéphane / Welfringer-Morin, Anne / Duffy, Darragh / Melki, Isabelle / Bader-Meunier, Brigitte

Journal of clinical immunology

2021 Volume 42, Issue 1, Page(s) 25–27

MeSH term(s)	Adolescent ; Asymptomatic Infections ; COVID-19/complications ; COVID-19/drug therapy ; Child ; Dermatomyositis/drug therapy ; Dermatomyositis/etiology ; Female ; Humans ; Immunoglobulin G/therapeutic use ; Immunoglobulin M/therapeutic use ; Inflammation/drug therapy ; Inflammation/etiology ; Inflammation/virology ; Recurrence
Chemical Substances	Immunoglobulin G ; Immunoglobulin M
Language	English
Publishing date	2021-08-23
Publishing country	Netherlands
Document type	Case Reports ; Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	779361-3
ISSN	1573-2592 ; 0271-9142
ISSN (online)	1573-2592
ISSN	0271-9142
DOI	10.1007/s10875-021-01119-y
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Article ; Online: Correction to: Onset and Relapse of Juvenile Dermatomyositis Following Asymptomatic SARS-CoV-2 Infection.

Rodero, Mathieu Paul / Pelleau, Stéphane / Welfringer-Morin, Anne / Duffy, Darragh / Melki, Isabelle / Bader-Meunier, Brigitte

Journal of clinical immunology

2021 Volume 42, Issue 1, Page(s) 28

Language	English
Publishing date	2021-10-07
Publishing country	Netherlands
Document type	Published Erratum
ZDB-ID	779361-3
ISSN	1573-2592 ; 0271-9142
ISSN (online)	1573-2592
ISSN	0271-9142
DOI	10.1007/s10875-021-01135-y
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Article ; Online: Declines in prevalence alter the optimal level of sexual investment for the malaria parasite

Early, Angela M / Camponovo, Flavia / Pelleau, Stéphane / Cerqueira, Gustavo C / Lazrek, Yassamine / Volney, Béatrice / Carrasquilla, Manuela / de Thoisy, Benoît / Buckee, Caroline O / Childs, Lauren M / Musset, Lise / Neafsey, Daniel E

Proceedings of the National Academy of Sciences of the United States of America

2022 Volume 119, Issue 30, Page(s) e2122165119

Abstract: Successful infectious disease interventions can result in large reductions in parasite prevalence. Such demographic change has fitness implications for individual parasites and may shift the parasite's optimal life history strategy. Here, we explore ... ...

Abstract	Successful infectious disease interventions can result in large reductions in parasite prevalence. Such demographic change has fitness implications for individual parasites and may shift the parasite's optimal life history strategy. Here, we explore whether declining infection rates can alter
MeSH term(s)	Animals ; Antimalarials/pharmacology ; Gene Frequency ; Humans ; Malaria, Falciparum/drug therapy ; Malaria, Falciparum/epidemiology ; Malaria, Falciparum/parasitology ; Models, Biological ; Plasmodium falciparum/drug effects ; Plasmodium falciparum/genetics ; Plasmodium falciparum/growth & development ; Prevalence
Chemical Substances	Antimalarials
Language	English
Publishing date	2022-07-22
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	209104-5
ISSN	1091-6490 ; 0027-8424
ISSN (online)	1091-6490
ISSN	0027-8424
DOI	10.1073/pnas.2122165119
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Article ; Online: Estimated protection against COVID-19 based on predicted neutralisation titres from multiple antibody measurements in a longitudinal cohort, France, April 2020 to November 2021.

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin

2023 Volume 28, Issue 25

Abstract: BackgroundThe risk of SARS-CoV-2 (re-)infection remains present given waning of vaccine-induced and infection-acquired immunity, and ongoing circulation of new variants.AimTo develop a method that predicts virus neutralisation and disease protection ... ...

Abstract	BackgroundThe risk of SARS-CoV-2 (re-)infection remains present given waning of vaccine-induced and infection-acquired immunity, and ongoing circulation of new variants.AimTo develop a method that predicts virus neutralisation and disease protection based on variant-specific antibody measurements to SARS-CoV-2 antigens.MethodsTo correlate antibody and neutralisation titres, we collected 304 serum samples from individuals with either vaccine-induced or infection-acquired SARS-CoV-2 immunity. Using the association between antibody and neutralisation titres, we developed a prediction model for SARS-CoV-2-specific neutralisation titres. From predicted neutralising titres, we inferred protection estimates to symptomatic and severe COVID-19 using previously described relationships between neutralisation titres and protection estimates. We estimated population immunity in a French longitudinal cohort of 905 individuals followed from April 2020 to November 2021.ResultsWe demonstrated a strong correlation between anti-SARS-CoV-2 antibodies measured using a low cost high-throughput assay and antibody response capacity to neutralise live virus. Participants with a single vaccination or immunity caused by infection were especially vulnerable to symptomatic or severe COVID-19. While the median reduced risk of COVID-19 from Delta variant infection in participants with three vaccinations was 96% (IQR: 94-98), median reduced risk among participants with infection-acquired immunity was only 42% (IQR: 22-66).ConclusionOur results are consistent with data from vaccine effectiveness studies, indicating the robustness of our approach. Our multiplex serological assay can be readily adapted to study new variants and provides a framework for development of an assay that would include protection estimates.
MeSH term(s)	Humans ; Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19/epidemiology ; France/epidemiology ; Reinfection ; SARS-CoV-2
Chemical Substances	Antibodies, Neutralizing ; Antibodies, Viral
Language	English
Publishing date	2023-06-21
Publishing country	Sweden
Document type	Journal Article
ZDB-ID	1338803-4
ISSN	1560-7917 ; 1025-496X
ISSN (online)	1560-7917
ISSN	1025-496X
DOI	10.2807/1560-7917.ES.2023.28.25.2200681
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Article: Kinetics of the SARS-CoV-2 Antibody Avidity Response Following Infection and Vaccination

Garcia, Laura / Woudenberg, Tom / Rosado, Jason / Dyer, Adam H. / Donnadieu, Françoise / Planas, Delphine / Bruel, Timothée / Schwartz, Olivier / Prazuck, Thierry / Velay, Aurélie / Fafi-Kremer, Samira / Batten, Isabella / Reddy, Conor / Connolly, Emma / McElheron, Matt / Kennelly, Sean P. / Bourke, Nollaig M. / White, Michael T. / Pelleau, Stéphane

Viruses. 2022 July 08, v. 14, no. 7

2022

Abstract: Serological assays capable of measuring antibody responses induced by previous infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been critical tools in the response to the COVID-19 pandemic. In this study, we use bead-based ...

Abstract	Serological assays capable of measuring antibody responses induced by previous infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been critical tools in the response to the COVID-19 pandemic. In this study, we use bead-based multiplex assays to measure IgG and IgA antibodies and IgG avidity to five SARS-CoV-2 antigens (Spike (S), receptor-binding domain (RBD), Nucleocapsid (N), S subunit 2, and Membrane-Envelope fusion (ME)). These assays were performed in several cohorts of healthcare workers and nursing home residents, who were followed for up to eleven months after SARS-CoV-2 infection or up to six months after vaccination. Our results show distinct kinetic patterns of antibody quantity (IgG and IgA) and avidity. While IgG and IgA antibody levels waned over time, with IgA antibody levels waning more rapidly, avidity increased with time after infection or vaccination. These contrasting kinetic patterns allow for the estimation of time since previous SARS-CoV-2 infection. Including avidity measurements in addition to antibody levels in a classification algorithm for estimating time since infection led to a substantial improvement in accuracy, from 62% to 78%. The inclusion of antibody avidity in panels of serological assays can yield valuable information for improving serosurveillance during SARS-CoV-2 epidemics.
Keywords	COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; algorithms ; antibodies ; antibody affinity ; health services ; nucleocapsid ; vaccination
Language	English
Dates of publication	2022-0708
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2516098-9
ISSN	1999-4915
ISSN	1999-4915
DOI	10.3390/v14071491
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Asymptomatic and symptomatic SARS-CoV-2 infections elicit polyfunctional antibodies.

Cell reports. Medicine

2021 Volume 2, Issue 5, Page(s) 100275

Abstract: Many SARS-CoV-2-infected individuals remain asymptomatic. Little is known about the extent and quality of their antiviral humoral response. Here, we analyze antibody functions in 52 asymptomatic infected individuals, 119 mildly symptomatic, and 21 ... ...

Abstract	Many SARS-CoV-2-infected individuals remain asymptomatic. Little is known about the extent and quality of their antiviral humoral response. Here, we analyze antibody functions in 52 asymptomatic infected individuals, 119 mildly symptomatic, and 21 hospitalized patients with COVID-19. We measure anti-spike immunoglobulin G (IgG), IgA, and IgM levels with the S-Flow assay and map IgG-targeted epitopes with a Luminex assay. We also evaluate neutralization, complement deposition, and antibody-dependent cellular cytotoxicity (ADCC) using replication-competent SARS-CoV-2 or reporter cell systems. We show that COVID-19 sera mediate complement deposition and kill infected cells by ADCC. Sera from asymptomatic individuals neutralize the virus, activate ADCC, and trigger complement deposition. Antibody levels and functions are lower in asymptomatic individuals than they are in symptomatic cases. Antibody functions are correlated, regardless of disease severity. Longitudinal samplings show that antibody functions follow similar kinetics of induction and contraction. Overall, asymptomatic SARS-CoV-2 infection elicits polyfunctional antibodies neutralizing the virus and targeting infected cells.
MeSH term(s)	Adolescent ; Adult ; Antibodies, Viral/blood ; Antibodies, Viral/immunology ; Antibody-Dependent Cell Cytotoxicity ; Antigen-Antibody Reactions ; Asymptomatic Diseases ; COVID-19/pathology ; COVID-19/virology ; Complement System Proteins/metabolism ; Epitopes/immunology ; Female ; Humans ; Immunoglobulin G/blood ; Immunoglobulin G/immunology ; Killer Cells, Natural/immunology ; Male ; Middle Aged ; Neutralization Tests ; SARS-CoV-2/isolation & purification ; SARS-CoV-2/metabolism ; Severity of Illness Index ; Spike Glycoprotein, Coronavirus/immunology ; Young Adult
Chemical Substances	Antibodies, Viral ; Epitopes ; Immunoglobulin G ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2 ; Complement System Proteins (9007-36-7)
Language	English
Publishing date	2021-04-20
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2666-3791
ISSN (online)	2666-3791
DOI	10.1016/j.xcrm.2021.100275
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Article ; Online: Kinetics of the SARS-CoV-2 Antibody Avidity Response Following Infection and Vaccination.

Garcia, Laura / Woudenberg, Tom / Rosado, Jason / Dyer, Adam H / Donnadieu, Françoise / Planas, Delphine / Bruel, Timothée / Schwartz, Olivier / Prazuck, Thierry / Velay, Aurélie / Fafi-Kremer, Samira / Batten, Isabella / Reddy, Conor / Connolly, Emma / McElheron, Matt / Kennelly, Sean P / Bourke, Nollaig M / White, Michael T / Pelleau, Stéphane

Viruses

2022 Volume 14, Issue 7

Abstract	Serological assays capable of measuring antibody responses induced by previous infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been critical tools in the response to the COVID-19 pandemic. In this study, we use bead-based multiplex assays to measure IgG and IgA antibodies and IgG avidity to five SARS-CoV-2 antigens (Spike (S), receptor-binding domain (RBD), Nucleocapsid (N), S subunit 2, and Membrane-Envelope fusion (ME)). These assays were performed in several cohorts of healthcare workers and nursing home residents, who were followed for up to eleven months after SARS-CoV-2 infection or up to six months after vaccination. Our results show distinct kinetic patterns of antibody quantity (IgG and IgA) and avidity. While IgG and IgA antibody levels waned over time, with IgA antibody levels waning more rapidly, avidity increased with time after infection or vaccination. These contrasting kinetic patterns allow for the estimation of time since previous SARS-CoV-2 infection. Including avidity measurements in addition to antibody levels in a classification algorithm for estimating time since infection led to a substantial improvement in accuracy, from 62% to 78%. The inclusion of antibody avidity in panels of serological assays can yield valuable information for improving serosurveillance during SARS-CoV-2 epidemics.
MeSH term(s)	Antibodies, Viral/immunology ; Antibody Affinity ; COVID-19/prevention & control ; COVID-19 Vaccines/administration & dosage ; Humans ; Immunoglobulin A ; Immunoglobulin G ; Kinetics ; Pandemics ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus ; Vaccination
Chemical Substances	Antibodies, Viral ; COVID-19 Vaccines ; Immunoglobulin A ; Immunoglobulin G ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
Language	English
Publishing date	2022-07-08
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v14071491
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Article ; Online: Neutralising antibody responses to SARS-CoV-2 omicron among elderly nursing home residents following a booster dose of BNT162b2 vaccine: A community-based, prospective, longitudinal cohort study.

EClinicalMedicine

2022 Volume 51, Page(s) 101576

Abstract: Background: The protective immunity against omicron following a BNT162b2 Pfizer booster dose among elderly individuals (ie, those aged >65 years) is not well characterised.: Methods: In a community-based, prospective, longitudinal cohort study taking ...

Abstract	Background: The protective immunity against omicron following a BNT162b2 Pfizer booster dose among elderly individuals (ie, those aged >65 years) is not well characterised. Methods: In a community-based, prospective, longitudinal cohort study taking place in France in which 75 residents from three nursing homes were enrolled, we selected 38 residents who had received a two-dose regimen of mRNA vaccine and a booster dose of Pfizer BNT162b2 vaccine. We excluded individuals that did not receive three vaccine doses or did not have available sera samples. We measured anti-S IgG antibodies and neutralisation capacity in sera taken 56 (28-68) and 55 (48-64) days (median (range)) after the 2 Findings: Among the 38 elderly individuals recruited to the cohort study between November 23 Interpretation: This study shows that elderly individuals who received three vaccine doses elicit neutralising antibodies against the omicron BA.1 variant of SARS-CoV-2. Elderly individuals who had also been previously infected showed higher neutralising activity compared with naive individuals. Yet, breakthrough infections with omicron occurred. Individuals with breakthrough infections had significantly lower neutralising titers compared to individuals without breakthrough infection. Thus, a fourth dose of vaccine may be useful in the elderly population to increase the level of neutralising antibodies and compensate for waning immunity. Funding: Institut Pasteur, Fondation pour la Recherche Médicale (FRM), European Health Emergency Preparedness and Response Authority (HERA), Agence nationale de recherches sur le sida et les hépatites virales - Maladies Infectieuses Emergentes (ANRS-MIE), Agence nationale de la recherche (ANR), Assistance Publique des Hôpitaux de Paris (AP-HP) and Fondation de France.
Language	English
Publishing date	2022-07-22
Publishing country	England
Document type	Journal Article
ISSN	2589-5370
ISSN (online)	2589-5370
DOI	10.1016/j.eclinm.2022.101576
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