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Article: Combination therapy with BMP-2 and psoralen enhances fracture healing in ovariectomized mice.

Huang, Kui / Wu, Guofeng / Zou, Ji / Peng, Songming

2018 Volume 16, Issue 3, Page(s) 1655–1662

Abstract: The advantages of combining local delivery of bone morphogenetic protein (BMP)-2 with systemic or local anti-osteoporosis treatments have also been studied for enhancing osteoporotic fracture healing. The aim of the present study was to evaluate the ... ...

Abstract	The advantages of combining local delivery of bone morphogenetic protein (BMP)-2 with systemic or local anti-osteoporosis treatments have also been studied for enhancing osteoporotic fracture healing. The aim of the present study was to evaluate the effect of combination therapy with BMP-2 and psoralen on fracture repair in ovariectomized mice. At 6 weeks after bilateral ovariectomy, mice (n=30) underwent unilateral transverse osteotomy on the femur and were divided into 3 groups. In the model group (n=10), animals were implanted with an absorbable collagen sponge (ACS) alone and administered physiological saline intragastrically (i.g.). In the recombinant human (rh)BMP-2 group (n=10), animals were implanted with an ACS loaded with 2.5 µg rhBMP-2 and administered physiological saline i.g. In the psoralen + rhBMP-2 group (n=10) animals were implanted with an ACS loaded with 2.5 µg rhBMP-2 and administered psoralen i.g. The mice were euthanized after 21 days and their fractured femurs were assessed by micro computed tomography, histological analysis and biomechanical testing. Furthermore, the serum of the animals was analyzed. Psoralen + rhBMP-2 exerted more beneficial effects on callus consolidation and biomechanical strength. In addition, increased bone-specific alkaline phosphatase levels and decreased C-terminal telopeptide of type-1 collagen were observed in the Psoralen + rhBMP-2 group. However, no difference in estrogen levels was detected between the groups. In conclusion, the present study demonstrated that in ovariectomized mice, combination of locally delivered BMP-2 and systemically administered psoralen improved bone healing compared with BMP-2 alone.
Language	English
Publishing date	2018-06-26
Publishing country	Greece
Document type	Journal Article
ZDB-ID	2683844-8
ISSN	1792-1015 ; 1792-0981
ISSN (online)	1792-1015
ISSN	1792-0981
DOI	10.3892/etm.2018.6353
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: An optically-gated AuNP-DNA protonic transistor.

Peng, Songming / Lal, Amit / Luo, Dan / Lu, Yuerui

Nanoscale

2017 Volume 9, Issue 21, Page(s) 6953–6958

Abstract: Bio-interface transistors, which manipulate the transportation of ions (i.e. protons), play an important role in bridging physical devices with biological functionalities, because electrical signals are carried by ions/protons in biological systems. All ... ...

Abstract	Bio-interface transistors, which manipulate the transportation of ions (i.e. protons), play an important role in bridging physical devices with biological functionalities, because electrical signals are carried by ions/protons in biological systems. All available ionic transistors use electrostatic gates to tune the ionic carrier density, which requires complicated interconnect wires. In contrast, an optical gate, which offers the advantages of remote control as well as multiple light wavelength selections, has never been explored for ionic devices. Here, we demonstrate a light-gated protonic transistor fabricated from an Au nanoparticle and DNA (AuNP-DNA) hybrid membrane. The device can be turned on and off completely by using light, with a high on/off current ratio of up to 2 orders of magnitude. Moreover, the device only responds to specific light wavelengths due to the plasmonic effect from the AuNPs, which enables the capability of wavelength selectivity. Our results open up new avenues for exploring remotely controlled ionic circuits, in vivo protonic switches, and other biomedical applications.
MeSH term(s)	DNA/chemistry ; Gold ; Ions ; Metal Nanoparticles/chemistry ; Protons ; Transistors, Electronic
Chemical Substances	Ions ; Protons ; Gold (7440-57-5) ; DNA (9007-49-2)
Language	English
Publishing date	2017-03-24
Publishing country	England
Document type	Journal Article
ZDB-ID	2515664-0
ISSN	2040-3372 ; 2040-3364
ISSN (online)	2040-3372
ISSN	2040-3364
DOI	10.1039/c6nr08944e
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Clinical Evaluation of Fused/Ankylosed Hip with Severe Flexion Deformity after Conversion to Total Hip Arthroplasty

Saroj Kumar Suwal / Peng Songming / Luo Gang / Huang Kui

Journal of Nepal Medical Association, Vol 54, Iss 202, Pp 63-

2016 Volume 66

Abstract: Introduction: Fused or Ankylosed hip is late complication of chronic inflammatory disorder with progressive changes in and around articular as well as periarticular structures with alteration in bio-force line of body which later lead to severe flexion ... ...

Abstract	Introduction: Fused or Ankylosed hip is late complication of chronic inflammatory disorder with progressive changes in and around articular as well as periarticular structures with alteration in bio-force line of body which later lead to severe flexion deformity of joint. This not only results decreased movements of hip, it’s also increase pain around the hip, back and contralateral hip. Methods: Retrospectively, all patients aged 18 years or older undergoing THA between June 2006 to June 2012 were reviewed with selection criteria. The five ankylosed hips (three left and two right) with severe flexion deformities which ankylosed spontaneously were successfully converted to THA at time period of 2006 to June 2012. Range of Motion (ROM), Harris Hip Score and Flexion Deformity Angle at preoperative, postoperative and follow-up periods were used as evaluation. Results: Mean follow up is 42 months. Mean HHS increased from 21.6±4.97 to 81.8±4.02 points with one excellent, two good and two fair cases. The FDA is corrected to mean 8°±10.95 postoperatively and 4°±5.47 at final follow up from 81.6°±4.39 with two hips of 10° residual deformity. Hip ROM is improved as flexion 70° to 100°, adduction 10° to 20°, abduction 10° to 30°, internal rotation 5° to 10° and external rotation 2° to 50° from 0° activity. As complications, one hip had loose prosthesis, two had early postoperative dislocations, one had Deep Vein Thrombosis and one had femoral nerve palsy with quadriceps weakness. Conclusions: THA is an effective treatment for ankylosed hip with severe flexion deformity although complications are noted more than routine hip arthroplasties. Keywords: ankylosed hip; fused hip; severe flexion deformity; total hip arthroplasty. \| PubMed
Keywords	Medicine (General) ; R5-920
Subject code	796
Language	English
Publishing date	2016-06-01T00:00:00Z
Publisher	Nepal Medical Association
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Clinical Evaluation of Fused/Ankylosed Hip with Severe Flexion Deformity after Conversion to Total Hip Arthroplasty

Saroj Kumar Suwal / Peng Songming / Luo Gang / Huang Kui

Journal of Nepal Medical Association, Vol 54, Iss

2016 Volume 202

Abstract	Introduction: Fused or Ankylosed hip is late complication of chronic inflammatory disorder with progressive changes in and around articular as well as periarticular structures with alteration in bio-force line of body which later lead to severe flexion deformity of joint. This not only results decreased movements of hip, it’s also increase pain around the hip, back and contralateral hip. Methods: Retrospectively, all patients aged 18 years or older undergoing THA between June 2006 to June 2012 were reviewed with selection criteria. The five ankylosed hips (three left and two right) with severe flexion deformities which ankylosed spontaneously were successfully converted to THA at time period of 2006 to June 2012. Range of Motion (ROM), Harris Hip Score and Flexion Deformity Angle at preoperative, postoperative and follow-up periods were used as evaluation. Results: Mean follow up is 42 months. Mean HHS increased from 21.6±4.97 to 81.8±4.02 points with one excellent, two good and two fair cases. The FDA is corrected to mean 8°±10.95 postoperatively and 4°±5.47 at final follow up from 81.6°±4.39 with two hips of 10° residual deformity. Hip ROM is improved as flexion 70° to 100°, adduction 10° to 20°, abduction 10° to 30°, internal rotation 5° to 10° and external rotation 2° to 50° from 0° activity. As complications, one hip had loose prosthesis, two had early postoperative dislocations, one had Deep Vein Thrombosis and one had femoral nerve palsy with quadriceps weakness. Conclusions: THA is an effective treatment for ankylosed hip with severe flexion deformity although complications are noted more than routine hip arthroplasties. Keywords: ankylosed hip; fused hip; severe flexion deformity; total hip arthroplasty. \| PubMed
Keywords	ankylosed hip ; fused hip ; severe flexion deformity ; total hip arthroplasty ; Medicine (General) ; R5-920
Subject code	796
Language	English
Publishing date	2016-06-01T00:00:00Z
Publisher	Nepal Medical Association
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Minimally invasive treatment of tibial pilon fractures through arthroscopy and external fixator-assisted reduction.

Luo, Huasong / Chen, Liaobin / Liu, Kebin / Peng, Songming / Zhang, Jien / Yi, Yang

SpringerPlus

2016 Volume 5, Issue 1, Page(s) 1923

Abstract: Objective: The aim of this study was to evaluate the clinical outcome of tibial pilon fractures treated with arthroscopy and assisted reduction with an external fixator.: Methods: Thirteen patients with tibial pilon fractures underwent assisted ... ...

Abstract	Objective: The aim of this study was to evaluate the clinical outcome of tibial pilon fractures treated with arthroscopy and assisted reduction with an external fixator. Methods: Thirteen patients with tibial pilon fractures underwent assisted reduction for limited lower internal fixation with an external fixator under arthroscopic guidance. The weight-bearing time was decided on the basis of repeat radiography of the tibia 3 months after surgery. Postoperative ankle function was evaluated according to the Mazur scoring system. Results: Healing of fractures was achieved in all cases, with no complications such as severe infection, skin necrosis, or an exposed plate. There were 9 excellent, 2 good, and 2 poor outcomes, scored according to the Mazur system. The acceptance rate was 85%. Conclusion: Arthroscopy and external fixator-assisted reduction for the minimally invasive treatment of tibial pilon fractures not only produced less trauma but also protected the soft tissues and blood supply surrounding the fractures. External fixation could indirectly provide reduction and effective operative space for arthroscopic implantation, especially for AO type B fractures and partial AO type C1 fractures.
Language	English
Publishing date	2016-11-05
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2661116-8
ISSN	2193-1801
ISSN	2193-1801
DOI	10.1186/s40064-016-3601-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Low-concentration mechanical biosensor based on a photonic crystal nanowire array.

Lu, Yuerui / Peng, Songming / Luo, Dan / Lal, Amit

Nature communications

2011 Volume 2, Page(s) 578

Abstract: The challenge for new biosensors is to achieve detection of biomolecules at low concentrations, which is useful for early-stage disease detection. Nanomechanical biosensors are promising in medical diagnostic applications. For nanomechanical biosensing ... ...

Abstract	The challenge for new biosensors is to achieve detection of biomolecules at low concentrations, which is useful for early-stage disease detection. Nanomechanical biosensors are promising in medical diagnostic applications. For nanomechanical biosensing at low concentrations, a sufficient resonator device surface area is necessary for molecules to bind to. Here we present a low-concentration (500 aM sensitivity) DNA sensor, which uses a novel nanomechanical resonator with ordered vertical nanowire arrays on top of a Si/SiO(2) bilayer thin membrane. The high sensitivity is achieved by the strongly enhanced total surface area-to-volume ratio of the resonator (10(8) m(-1)) and the state-of-the-art mass-per-area resolution (1.8×10(-12) kg m(-2)). Moreover, the nanowire array forms a photonic crystal that shows strong light trapping and absorption over broad-band optical wavelengths, enabling high-efficiency broad-band opto-thermo-mechanical remote device actuation and biosensing on a chip. This method represents a mass-based platform technology that can sense molecules at low concentrations.
MeSH term(s)	Biosensing Techniques/instrumentation ; Biosensing Techniques/methods ; Crystallization ; DNA/analysis ; DNA Probes/chemistry ; Equipment Design ; Nanotechnology/instrumentation ; Nanotechnology/methods ; Nanowires/chemistry ; Optical Devices ; Photochemistry ; Photons ; Sensitivity and Specificity ; Silicon Dioxide/chemistry
Chemical Substances	DNA Probes ; Silicon Dioxide (7631-86-9) ; DNA (9007-49-2)
Language	English
Publishing date	2011-12-06
Publishing country	England
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/ncomms1587
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: DNA Microgels as a Platform for Cell-Free Protein Expression and Display

Kahn, JasonS / An, Duo / Derrien, Thomas L / Luo, Dan / Peng, Songming / Ruiz, Roanna C. H / Sureka, Swati

Biomacromolecules. 2016 June 13, v. 17, no. 6

2016

Abstract: Protein expression and selection is an essential process in the modification of biological products. Expressed proteins are selected based on desired traits (phenotypes) from diverse gene libraries (genotypes), whose size may be limited due to the ... ...

Abstract	Protein expression and selection is an essential process in the modification of biological products. Expressed proteins are selected based on desired traits (phenotypes) from diverse gene libraries (genotypes), whose size may be limited due to the difficulties inherent in diverse cell preparation. In addition, not all genes can be expressed in cells, and linking genotype with phenotype further presents a great challenge in protein engineering. We present a DNA gel-based platform that demonstrates the versatility of two DNA microgel formats to address fundamental challenges of protein engineering, including high protein yield, isolation of gene sets, and protein display. We utilize microgels to show successful protein production and capture of a model protein, green fluorescent protein (GFP), which is further used to demonstrate a successful gene enrichment through fluorescence-activated cell sorting (FACS) of a mixed population of microgels containing the GFP gene. Through psoralen cross-linking of the hydrogels, we have synthesized DNA microgels capable of surviving denaturing conditions while still possessing the ability to produce protein. Lastly, we demonstrate a method of producing extremely high local gene concentrations of up to 32 000 gene repeats in hydrogels 1 to 2 μm in diameter. These DNA gels can serve as a novel cell-free platform for integrated protein expression and display, which can be applied toward more powerful, scalable protein engineering and cell-free synthetic biology with no physiological boundaries and limitations.
Keywords	cell-free protein synthesis ; crosslinking ; DNA ; DNA libraries ; flow cytometry ; genes ; genotype ; green fluorescent protein ; hydrogels ; models ; phenotype ; protein engineering ; psoralen ; synthetic biology
Language	English
Dates of publication	2016-0613
Size	p. 2019-2026.
Publishing place	American Chemical Society
Document type	Article
ISSN	1526-4602
DOI	10.1021%2Facs.biomac.6b00183
Database	NAL-Catalogue (AGRICOLA)

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Article: Large libraries of single-chain trimer peptide-MHCs enable rapid antigen-specific CD8+ T cell discovery and analysis.

Research square

2022

Abstract: CD8 + cytotoxic T cell responses against viral infection represent a major element of the adaptive immune response. We describe the development of a peptide antigen - major histompatibility complex (pMHC) library representing the full SARS-CoV-2 viral ... ...

Abstract	CD8 + cytotoxic T cell responses against viral infection represent a major element of the adaptive immune response. We describe the development of a peptide antigen - major histompatibility complex (pMHC) library representing the full SARS-CoV-2 viral proteome, and comprised of 634 pMHC multimers representing the A02.01, A24.02, and B*07.02 HLA alleles, as well as specific antigens associated with the cytomegalovirus (CMV). These libraries were used to capture non-expanded CD8 + T cells from blood samples collected from 64 infected individuals, and then analyzed using single cell RNA-seq. The discovery and characterization of antigen-specific CD8
Language	English
Publishing date	2022-11-18
Publishing country	United States
Document type	Preprint
DOI	10.21203/rs.3.rs-1090664/v1
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Article ; Online: Large libraries of single-chain trimer peptide-MHCs enable antigen-specific CD8+ T cell discovery and analysis.

Chour, William / Choi, Jongchan / Xie, Jingyi / Chaffee, Mary E / Schmitt, Thomas M / Finton, Kathryn / DeLucia, Diana C / Xu, Alexander M / Su, Yapeng / Chen, Daniel G / Zhang, Rongyu / Yuan, Dan / Hong, Sunga / Ng, Alphonsus H C / Butler, Jonah Z / Edmark, Rick A / Jones, Lesley C / Murray, Kim M / Peng, Songming /

Li, Guideng / Strong, Roland K / Lee, John K / Goldman, Jason D / Greenberg, Philip D / Heath, James R

Communications biology

2023 Volume 6, Issue 1, Page(s) 528

Abstract: The discovery and characterization of antigen-specific ... ...

Abstract	The discovery and characterization of antigen-specific CD8
MeSH term(s)	Humans ; CD8-Positive T-Lymphocytes ; COVID-19 ; SARS-CoV-2/genetics ; Antigens ; Epitopes ; Peptides/genetics
Chemical Substances	Antigens ; Epitopes ; Peptides
Language	English
Publishing date	2023-05-16
Publishing country	England
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural
ISSN	2399-3642
ISSN (online)	2399-3642
DOI	10.1038/s42003-023-04899-8
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Article ; Online: In vitro 3D human small intestinal villous model for drug permeability determination.

Yu, Jiajie / Peng, Songming / Luo, Dan / March, John C

Biotechnology and bioengineering

2012 Volume 109, Issue 9, Page(s) 2173–2178

Abstract: We present a novel method for testing drug permeability that features human cells cultured on hydrogel scaffolds made to accurately replicate the shape and size of human small intestinal villi. We compared villous scaffolds to more conventional 2D ... ...

Abstract	We present a novel method for testing drug permeability that features human cells cultured on hydrogel scaffolds made to accurately replicate the shape and size of human small intestinal villi. We compared villous scaffolds to more conventional 2D cultures in paracellular drug absorption and cell growth experiments. Our results suggest that 3D villous platforms facilitate cellular differentiation and absorption more similar to mammalian intestines than can be achieved using conventional culture. To the best of our knowledge, this is the first accurate 3D villus model offering a well-controlled microenvironment that has strong physiological relevance to the in vivo system.
MeSH term(s)	Artificial Organs ; Caco-2 Cells ; Cell Culture Techniques/instrumentation ; Cell Culture Techniques/methods ; Humans ; Intestinal Absorption ; Intestinal Mucosa/anatomy & histology ; Intestinal Mucosa/cytology ; Intestinal Mucosa/metabolism ; Intestine, Small/cytology ; Intestine, Small/metabolism ; Microvilli/metabolism ; Pharmacokinetics
Language	English
Publishing date	2012-09
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	280318-5
ISSN	1097-0290 ; 0006-3592
ISSN (online)	1097-0290
ISSN	0006-3592
DOI	10.1002/bit.24518
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