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  1. Article ; Online: Monitoring redox stress in human airway epithelial cells exposed to woodsmoke at an air-liquid interface.

    Abzhanova, Aiman / Berntsen, Jon / Pennington, Edward R / Dailey, Lisa / Masood, Syed / George, Ingrid / Warren, Nina / Martin, Joseph / Hays, Michael D / Ghio, Andrew J / Weinstein, Jason P / Kim, Yong Ho / Puckett, Earl / Samet, James M

    Particle and fibre toxicology

    2024  Volume 21, Issue 1, Page(s) 14

    Abstract: Wildland fires contribute significantly to the ambient air pollution burden worldwide, causing a range of adverse health effects in exposed populations. The toxicity of woodsmoke, a complex mixture of gases, volatile organic compounds, and particulate ... ...

    Abstract Wildland fires contribute significantly to the ambient air pollution burden worldwide, causing a range of adverse health effects in exposed populations. The toxicity of woodsmoke, a complex mixture of gases, volatile organic compounds, and particulate matter, is commonly studied in vitro using isolated exposures of conventionally cultured lung cells to either resuspended particulate matter or organic solvent extracts of smoke, leading to incomplete toxicity evaluations. This study aimed to improve our understanding of the effects of woodsmoke inhalation by building an advanced in vitro exposure system that emulates human exposure of the airway epithelium. We report the development and characterization of an innovative system that permits live-cell monitoring of the intracellular redox status of differentiated primary human bronchial epithelial cells cultured at an air-liquid interface (pHBEC-ALI) as they are exposed to unfractionated woodsmoke generated in a tube furnace in real time. pHBEC-ALI exposed to freshly generated woodsmoke showed oxidative changes that were dose-dependent and reversible, and not attributable to carbon monoxide exposure. These findings show the utility of this novel system for studying the molecular initiating events underlying woodsmoke-induced toxicity in a physiologically relevant in vitro model, and its potential to provide biological plausibility for risk assessment and public health measures.
    MeSH term(s) Humans ; Particulate Matter/toxicity ; Smoke/adverse effects ; Air Pollution ; Lung ; Epithelial Cells
    Chemical Substances Particulate Matter ; Smoke
    Language English
    Publishing date 2024-03-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 2170936-1
    ISSN 1743-8977 ; 1743-8977
    ISSN (online) 1743-8977
    ISSN 1743-8977
    DOI 10.1186/s12989-024-00575-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Improvement in Fitted Filtration Efficiency of N95 Respirators With Escalating Instruction of the Wearer.

    Chen, Hao / Pennington, Edward R / Case, Martin W / Tong, Haiyan / Rappold, Ana G / Samet, James M / Prince, Steven E

    AJPM focus

    2022  Volume 1, Issue 1, Page(s) 100014

    Abstract: Introduction: Fitted filtration performance of an N95 respirator may benefit from differing levels of instructions.: Methods: Using a modified Occupational Safety and Health Administration fit test protocol, we measured fitted filtration efficiency ... ...

    Abstract Introduction: Fitted filtration performance of an N95 respirator may benefit from differing levels of instructions.
    Methods: Using a modified Occupational Safety and Health Administration fit test protocol, we measured fitted filtration efficiency for an N95 respirator in 21 screened, healthy participants given 4 levels of escalating instruction: (1) uninstructed (baseline), (2) written/pictorial manufacturer instructions, (3) step-by-step video demonstration, and (4) staff instruction (visual inspection of respirator fit and verbal suggestions to adjust when applicable).
    Results: Baseline fitted filtration efficiency was not significantly different between participants with or without previous experience of N95 use. Clear improvements in fitted filtration efficiency were observed progressing from baseline (average=86.1%) to manufacturer paper instructions (93.3%), video instructions (97.5%), and post staff intervention (98.3%). Baseline fitted filtration efficiency values were significantly lower than those after video instruction (p<0.037) and staff intervention (p<0.033) sessions.
    Conclusions: Beyond uninstructed wear or provision of manufacturer instructions, efforts to train and instruct users in proper respirator fit principles with visual feedback are likely to yield benefits to public health outcomes in reducing respiratory exposure during air quality emergencies such as airborne viral outbreaks and wildland fires.
    Language English
    Publishing date 2022-07-21
    Publishing country England
    Document type Journal Article
    ISSN 2773-0654
    ISSN (online) 2773-0654
    DOI 10.1016/j.focus.2022.100014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Non-redox cycling mechanisms of oxidative stress induced by PM metals.

    Samet, James M / Chen, Hao / Pennington, Edward R / Bromberg, Philip A

    Free radical biology & medicine

    2019  Volume 151, Page(s) 26–37

    Abstract: Metallic compounds contribute to the oxidative stress of ambient particulate matter (PM) exposure. The toxicity of redox inert ions of cadmium, mercury, lead and zinc, as well as redox-active ions of vanadium and chromium is underlain by dysregulation of ...

    Abstract Metallic compounds contribute to the oxidative stress of ambient particulate matter (PM) exposure. The toxicity of redox inert ions of cadmium, mercury, lead and zinc, as well as redox-active ions of vanadium and chromium is underlain by dysregulation of mitochondrial function and loss of signaling quiescence. Central to the initiation of these effects is the interaction of metal ions with cysteinyl thiols on glutathione and key regulatory proteins, which leads to impaired mitochondrial electron transport and persistent pan-activation of signal transduction pathways. The mitochondrial and signaling effects are linked by the production of H
    MeSH term(s) Glutathione ; Hydrogen Peroxide ; Metals/toxicity ; Oxidation-Reduction ; Oxidative Stress
    Chemical Substances Metals ; Hydrogen Peroxide (BBX060AN9V) ; Glutathione (GAN16C9B8O)
    Language English
    Publishing date 2019-12-23
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 807032-5
    ISSN 1873-4596 ; 0891-5849
    ISSN (online) 1873-4596
    ISSN 0891-5849
    DOI 10.1016/j.freeradbiomed.2019.12.027
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Real-time redox adaptations in human airway epithelial cells exposed to isoprene hydroxy hydroperoxide.

    Pennington, Edward R / Masood, Syed / Simmons, Steven O / Dailey, Lisa / Bromberg, Philip A / Rice, Rebecca L / Gold, Avram / Zhang, Zhenfa / Wu, Weidong / Yang, Yi / Samet, James M

    Redox biology

    2023  Volume 61, Page(s) 102646

    Abstract: While redox processes play a vital role in maintaining intracellular homeostasis by regulating critical signaling and metabolic pathways, supra-physiological or sustained oxidative stress can lead to adverse responses or cytotoxicity. Inhalation of ... ...

    Abstract While redox processes play a vital role in maintaining intracellular homeostasis by regulating critical signaling and metabolic pathways, supra-physiological or sustained oxidative stress can lead to adverse responses or cytotoxicity. Inhalation of ambient air pollutants such as particulate matter and secondary organic aerosols (SOA) induces oxidative stress in the respiratory tract through mechanisms that remain poorly understood. We investigated the effect of isoprene hydroxy hydroperoxide (ISOPOOH), an atmospheric oxidation product of vegetation-derived isoprene and a constituent of SOA, on intracellular redox homeostasis in cultured human airway epithelial cells (HAEC). We used high-resolution live cell imaging of HAEC expressing the genetically encoded ratiometric biosensors Grx1-roGFP2, iNAP1, or HyPer, to assess changes in the cytoplasmic ratio of oxidized glutathione to reduced glutathione (GSSG:GSH), and the flux of NADPH and H
    MeSH term(s) Humans ; Hydrogen Peroxide/pharmacology ; Glutathione Disulfide/metabolism ; Oxidation-Reduction ; Glutathione/metabolism ; Epithelial Cells/metabolism ; Oxidative Stress ; Respiratory System/metabolism ; Glucose/pharmacology ; NADP/metabolism
    Chemical Substances Hydrogen Peroxide (BBX060AN9V) ; Glutathione Disulfide (ULW86O013H) ; isoprene (0A62964IBU) ; Glutathione (GAN16C9B8O) ; Glucose (IY9XDZ35W2) ; NADP (53-59-8)
    Language English
    Publishing date 2023-02-25
    Publishing country Netherlands
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2701011-9
    ISSN 2213-2317 ; 2213-2317
    ISSN (online) 2213-2317
    ISSN 2213-2317
    DOI 10.1016/j.redox.2023.102646
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Live cell imaging of oxidative stress in human airway epithelial cells exposed to isoprene hydroxyhydroperoxide.

    Masood, Syed / Pennington, Edward R / Simmons, Steven O / Bromberg, Philip A / Shaikh, Saame R / Rice, Rebecca L / Gold, Avram / Zhang, Zhenfa / Samet, James M

    Redox biology

    2022  Volume 51, Page(s) 102281

    Abstract: Exposure to respirable air particulate matter ( ... ...

    Abstract Exposure to respirable air particulate matter (PM
    MeSH term(s) Butadienes ; Epithelial Cells/metabolism ; Glutathione/metabolism ; Hemiterpenes ; Humans ; Hydrogen Peroxide/pharmacology ; Oxidants/pharmacology ; Oxidation-Reduction ; Oxidative Stress ; Particulate Matter
    Chemical Substances Butadienes ; Hemiterpenes ; Oxidants ; Particulate Matter ; isoprene (0A62964IBU) ; Hydrogen Peroxide (BBX060AN9V) ; Glutathione (GAN16C9B8O)
    Language English
    Publishing date 2022-03-15
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2701011-9
    ISSN 2213-2317 ; 2213-2317
    ISSN (online) 2213-2317
    ISSN 2213-2317
    DOI 10.1016/j.redox.2022.102281
    Database MEDical Literature Analysis and Retrieval System OnLINE

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