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  1. Article ; Online: Gold perfusion experiments support the multi-layered, mesoporous nature of intervessel pit membranes in angiosperm xylem.

    Zhang, Ya / Pereira, Luciano / Kaack, Lucian / Liu, Jiabao / Jansen, Steven

    The New phytologist

    2024  Volume 242, Issue 2, Page(s) 493–506

    Abstract: Fluid transport across intervessel pit membranes of angiosperm xylem plays a major role in plant transpiration, with transport resistance largely depending on pore constriction sizes. Traditionally, fluid particles traversing pit membranes are assumed to ...

    Abstract Fluid transport across intervessel pit membranes of angiosperm xylem plays a major role in plant transpiration, with transport resistance largely depending on pore constriction sizes. Traditionally, fluid particles traversing pit membranes are assumed to cross a single instead of multiple pore constrictions. We tested a multi-layered pit membrane model in xylem of eight angiosperm species by estimating the size frequency of pore constrictions in relation to pit membrane thickness and compared modelled data with perfusion characteristics of nanoscale gold particles based on transmission electron microscopy. The size frequency of modelled pore constrictions showed similar patterns to the measured number of perfused particle sizes inside pit membranes, although frequency values measured were 10-50 times below modelled data. Small particles enter pit membranes most easily, especially when injected in thin pit membranes. The trapping of gold particles by pore constrictions becomes more likely with increasing pore constriction number and pit membrane thickness. While quantitative differences between modelled and experimental data are due to various practical limitations, their qualitative agreement supports a multi-layered pit membrane model with multiple pore constrictions. Pore constrictions between 5 and 50 nm are realistic, and confirm the mesoporous nature of pit membranes.
    MeSH term(s) Gold ; Magnoliopsida ; Xylem ; Biological Transport ; Perfusion ; Water
    Chemical Substances Gold (7440-57-5) ; Water (059QF0KO0R)
    Language English
    Publishing date 2024-02-25
    Publishing country England
    Document type Journal Article
    ZDB-ID 208885-x
    ISSN 1469-8137 ; 0028-646X
    ISSN (online) 1469-8137
    ISSN 0028-646X
    DOI 10.1111/nph.19608
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Current and Emerging Markers and Tools Used in the Diagnosis and Management of Chronic Kidney Disease-Mineral and Bone Disorder in Non-Dialysis Adult Patients.

    Fusaro, Maria / Pereira, Luciano / Bover, Jordi

    Journal of clinical medicine

    2023  Volume 12, Issue 19

    Abstract: Chronic kidney disease (CKD) is a significant public health concern associated with significant morbidity and has become one of the foremost global causes of death in recent years. A frequent comorbidity of CKD is secondary hyperparathyroidism (SHPT), ... ...

    Abstract Chronic kidney disease (CKD) is a significant public health concern associated with significant morbidity and has become one of the foremost global causes of death in recent years. A frequent comorbidity of CKD is secondary hyperparathyroidism (SHPT), exemplified by high serum parathyroid hormone (PTH) levels. The mineral metabolism disturbances resulting from CKD and progression to SHPT are currently considered part of the definition of chronic kidney disease-mineral and bone disorder (CKD-MBD). However, CKD-MBD does not only include abnormalities in laboratory-measured parameters; it is a complex condition characterized by dysregulation of bone turnover, mineralization, growth and strength, accompanied by vascular or another soft-tissue calcification. Together, this increases the risk of bone fractures, cardiovascular disease, and overall mortality in CKD-MBD patients. Monitoring serum markers is essential in diagnosing SHPT and CKD-MBD, and there are several recognized indicators for prognosis, optimal clinical management and treatment response in late-stage kidney disease patients receiving dialysis. However, far fewer markers have been established for patients with non-dialysis CKD. This review provides an overview of current and emerging markers and tools used in the diagnosis and management of CKD-MBD in non-dialysis adult patients.
    Language English
    Publishing date 2023-09-30
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm12196306
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Game-based learning enhances students' understanding of endocrine physiology in veterinary medicine.

    Afonso, Marcos Vinícius Ramos / Lopes, Rodrigo Braga / Andrade, Eric Francelino / Pereira, Luciano José

    Advances in physiology education

    2024  Volume 48, Issue 2, Page(s) 155–163

    Abstract: Endocrine physiology is a complex subject for students. Game-based learning (GBL) and case-based learning (CBL) are active methodologies that are widely used because of their potential for motivation and greater proximity to the reality of modern ... ...

    Abstract Endocrine physiology is a complex subject for students. Game-based learning (GBL) and case-based learning (CBL) are active methodologies that are widely used because of their potential for motivation and greater proximity to the reality of modern students. We evaluated the effectiveness of GBL and CBL among veterinary medicine students compared with a control group using peer tutoring. Students (
    MeSH term(s) Humans ; Learning ; Students ; Motivation ; Problem Solving ; Surveys and Questionnaires
    Language English
    Publishing date 2024-01-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1024917-5
    ISSN 1522-1229 ; 1043-4046
    ISSN (online) 1522-1229
    ISSN 1043-4046
    DOI 10.1152/advan.00182.2023
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Corrigendum to "Surface-guided radiotherapy for lung cancer can reduce the number of close patient contacts without compromising initial setup accuracy" [Tech. Innov. Patient Support Radiat. Oncol. 20 (2021) 61-63].

    Blake, Nicola / Pereira, Luciano / Eaton, David J / Dobson, Deirdre

    Technical innovations & patient support in radiation oncology

    2023  Volume 25, Page(s) 100197

    Abstract: This corrects the article DOI: 10.1016/j.tipsro.2021.11.005.]. ...

    Abstract [This corrects the article DOI: 10.1016/j.tipsro.2021.11.005.].
    Language English
    Publishing date 2023-01-07
    Publishing country England
    Document type Published Erratum
    ISSN 2405-6324
    ISSN (online) 2405-6324
    DOI 10.1016/j.tipsro.2022.12.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Physical exercise and peritoneal dialysis: An area yet to be explored.

    Maia Neves Menezes, José Inacio / Lopes Pereira, Luciano Artur

    Nefrologia

    2022  Volume 42, Issue 3, Page(s) 265–272

    Abstract: Health-related quality of life (HRQoL) of patients suffering from chronic kidney disease (CKD) is profoundly impaired by their frailty, disability and decreased physical capacity. Especially among older patients, a high prevalence of low physical ... ...

    Abstract Health-related quality of life (HRQoL) of patients suffering from chronic kidney disease (CKD) is profoundly impaired by their frailty, disability and decreased physical capacity. Especially among older patients, a high prevalence of low physical activity levels and reduced functional performance has been reported. Physical exercise training has been shown to have a beneficial impact, counteracting these same hazardous consequences of inactivity and sedentarism both on CKD and end-stage kidney disease (ESKD) patients on hemodialysis (HD) treatment. The evidence-based knowledge on the effects of physical exercise on ESKD patients undergoing Peritoneal Dialysis (PD) treatment is scarce, even though this is a continually growing population that shares the same risk factors and desired clinical outcomes as the previously mentioned groups of patients. Further investigation will be necessary to clarify whether this exercise-based approach may be suitable for the PD population. This paper's purpose is to review the available literature, including randomized controlled trials, reviews and meta-analysis results that assessed the impact of physical exercise on patients under PD treatment bearing in mind their HRQoL, physical functioning and cardiovascular parameters. Furthermore, it aims to evaluate the perceived significant barriers and limitations of the PD population in what concerns physical exercise practice and how nephrologists should address them.
    MeSH term(s) Exercise ; Humans ; Kidney Failure, Chronic/etiology ; Peritoneal Dialysis/adverse effects ; Quality of Life ; Renal Insufficiency, Chronic/etiology ; Renal Insufficiency, Chronic/therapy
    Language English
    Publishing date 2022-10-06
    Publishing country Spain
    Document type Journal Article ; Meta-Analysis ; Review
    ZDB-ID 2837917-2
    ISSN 2013-2514 ; 2013-2514
    ISSN (online) 2013-2514
    ISSN 2013-2514
    DOI 10.1016/j.nefroe.2021.02.012
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: The bone-vessel axis in chronic kidney disease: An update on biochemical players and its future role in laboratory medicine.

    Pereira, Luciano / Frazão, João M

    Clinica chimica acta; international journal of clinical chemistry

    2020  Volume 508, Page(s) 221–227

    Abstract: Vascular wall calcification (VC) is highly prevalent in patients with chronic kidney disease (CKD). In CKD, VC is more frequent and severe than in the general population and it is associated with increased cardiovascular mortality and morbidity. In the ... ...

    Abstract Vascular wall calcification (VC) is highly prevalent in patients with chronic kidney disease (CKD). In CKD, VC is more frequent and severe than in the general population and it is associated with increased cardiovascular mortality and morbidity. In the last years, laboratory and clinical evidence have drawn the attention to the relationship between bone disease and VC in CKD patients, leading to the concept of a bone-vessel or bone-vascular axis. It means that disorders of bone volume and bone turnover may influence the risk of VC and ultimately the high risk of cardiovascular mortality. In fact, a higher burden of VC has been associated to low bone volume and low bone turnover in hemodialysis (HD) patients with renal osteodystrophy characterized by histomorphometric evaluation of bone biopsies. The molecular mechanisms underlying the regulation of bone cells and vascular cells in CKD are poorly understood. In this review, we discuss relevant evidence linking bone disorders and VC in CKD and also rising molecular players involved in this bone-vascular axis. Indeed, accumulating data is available for two proposed systems: receptor activator for nuclear factor kB (RANK)/ RANK ligand (RANKL)/osteoprotegerin (OPG) system and inhibitors of Wnt signaling - mainly sclerostin. Although they are promising biochemical markers linking bone formation and bone reabsorption with VC, there is a long way to go as long evidence from laboratory studies is often divergent to the clinical data as will be discussed. Future prospective studies are needed in order to evaluate the role of these biochemical players as useful clinical markers for VC, bone volume and perhaps bone turnover.
    MeSH term(s) Bone and Bones ; Chronic Kidney Disease-Mineral and Bone Disorder/diagnosis ; Humans ; Laboratories ; Prospective Studies ; Renal Insufficiency, Chronic ; Vascular Calcification
    Language English
    Publishing date 2020-05-15
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 80228-1
    ISSN 1873-3492 ; 0009-8981
    ISSN (online) 1873-3492
    ISSN 0009-8981
    DOI 10.1016/j.cca.2020.05.023
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Physical exercise and peritoneal dialysis: An area yet to be explored.

    Maia Neves Menezes, José Inacio / Lopes Pereira, Luciano Artur

    Nefrologia

    2021  

    Abstract: Health-related quality of life (HRQoL) of patients suffering from chronic kidney disease (CKD) is profoundly impaired by their frailty, disability and decreased physical capacity. Especially among older patients, a high prevalence of low physical ... ...

    Abstract Health-related quality of life (HRQoL) of patients suffering from chronic kidney disease (CKD) is profoundly impaired by their frailty, disability and decreased physical capacity. Especially among older patients, a high prevalence of low physical activity levels and reduced functional performance has been reported. Physical exercise training has been shown to have a beneficial impact, counteracting these same hazardous consequences of inactivity and sedentarism both on CKD and end-stage kidney disease (ESKD) patients on hemodialysis (HD) treatment. The evidence-based knowledge on the effects of physical exercise on ESKD patients undergoing Peritoneal Dialysis (PD) treatment is scarce, even though this is a continually growing population that shares the same risk factors and desired clinical outcomes as the previously mentioned groups of patients. Further investigation will be necessary to clarify whether this exercise-based approach may be suitable for the PD population. This paper's purpose is to review the available literature, including randomized controlled trials, reviews and meta-analysis results that assessed the impact of physical exercise on patients under PD treatment bearing in mind their HRQoL, physical functioning and cardiovascular parameters. Furthermore, it aims to evaluate the perceived significant barriers and limitations of the PD population in what concerns physical exercise practice and how nephrologists should address them.
    Language Spanish
    Publishing date 2021-08-18
    Publishing country Spain
    Document type Journal Article ; Review
    ZDB-ID 2837917-2
    ISSN 2013-2514 ; 2013-2514
    ISSN (online) 2013-2514
    ISSN 2013-2514
    DOI 10.1016/j.nefro.2021.02.007
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: The urogenital microbiome in chronic kidney disease patients on peritoneal dialysis.

    Araujo, Ricardo / Merino-Ribas, Ana / Pereira, Luciano / Campos, Joana / Silva, Nádia / Alencastre, Inês Soares / Pestana, Manuel / Sampaio-Maia, Benedita

    Nefrologia

    2024  Volume 44, Issue 2, Page(s) 194–203

    Abstract: Introduction and objectives: Diabetes, dyslipidemia, older age, gender, urinary tract infections, and recent antibiotic intake have been associated with a decrease in the urobiome richness and other fluctuations in this microbiome. Gut and blood ... ...

    Abstract Introduction and objectives: Diabetes, dyslipidemia, older age, gender, urinary tract infections, and recent antibiotic intake have been associated with a decrease in the urobiome richness and other fluctuations in this microbiome. Gut and blood microbiome have been reported to be altered in patients with chronic kidney disease (CKD), and specifically in peritoneal dialysis (PD) patients. Still, there are currently no studies describing the urogenital microbiome in CKD-PD patients. In this study we characterized the urobiome profile in 46 PD patients and analyzed its clinical and inflammatory parameters.
    Materials and methods: Mid-stream urine, fecal and blood samples were collected from 46 patients undergoing PD at Centro Hospitalar Universitário de São João (CHUSJ) in Porto, Portugal. Exclusion criteria were age under 18 years old, inability to give informed consent, history of infection in the last three months, and antibiotic intake in the last three months. The microbiome communities were analyzed by amplification and sequencing of the V3-V4 region of the bacterial 16S rRNA gene. Correlations with the patients' clinical data and inflammatory profile were performed.
    Results: CKD-PD patients presented a unique urobiome profile dominated by Bacillota, Actinomycetota and Pseudomonadota and characterized by a lower Shannon diversity than fecal and blood microbiome. The taxonomic profiles of urogenital samples were organized in multiple subtypes dominated by populations of Lactobacillus, Staphylococcus, Streptococcus, Gardnerella, Prevotella, Escherichia-Shigella, being similar to other non-PD-CKD patients. Gender, sCD14, residual diuresis and history of peritonitis were significantly associated to variations in the urobiome. Although not reaching statistical significance, diabetes and the time on PD also showed association with particular taxonomic groups. Depletion of Gardnerella, Staphylococcus, Corynebacterium, Lactobacillus or Dermabacter populations correlated with CKD-PD patients with history of diabetes, history of peritonitis and altered levels of sCD14.
    Conclusions: Our results highlight urogenital microbiome as a potential partner and/or marker in the overall health state of CKD-PD patients.
    MeSH term(s) Humans ; Female ; Male ; Peritoneal Dialysis/adverse effects ; Middle Aged ; Microbiota ; Renal Insufficiency, Chronic/microbiology ; Renal Insufficiency, Chronic/therapy ; Renal Insufficiency, Chronic/complications ; Aged ; Urogenital System/microbiology ; Adult ; Feces/microbiology
    Language English
    Publishing date 2024-05-02
    Publishing country Spain
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2837917-2
    ISSN 2013-2514 ; 2013-2514
    ISSN (online) 2013-2514
    ISSN 2013-2514
    DOI 10.1016/j.nefroe.2024.04.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Estimation of pure quantum states in high dimension at the limit of quantum accuracy through complex optimization and statistical inference.

    Zambrano, Leonardo / Pereira, Luciano / Niklitschek, Sebastián / Delgado, Aldo

    Scientific reports

    2020  Volume 10, Issue 1, Page(s) 12781

    Abstract: Quantum tomography has become a key tool for the assessment of quantum states, processes, and devices. This drives the search for tomographic methods that achieve greater accuracy. In the case of mixed states of a single 2-dimensional quantum system ... ...

    Abstract Quantum tomography has become a key tool for the assessment of quantum states, processes, and devices. This drives the search for tomographic methods that achieve greater accuracy. In the case of mixed states of a single 2-dimensional quantum system adaptive methods have been recently introduced that achieve the theoretical accuracy limit deduced by Hayashi and Gill and Massar. However, accurate estimation of higher-dimensional quantum states remains poorly understood. This is mainly due to the existence of incompatible observables, which makes multiparameter estimation difficult. Here we present an adaptive tomographic method and show through numerical simulations that, after a few iterations, it is asymptotically approaching the fundamental Gill-Massar lower bound for the estimation accuracy of pure quantum states in high dimension. The method is based on a combination of stochastic optimization on the field of the complex numbers and statistical inference, exceeds the accuracy of any mixed-state tomographic method, and can be demonstrated with current experimental capabilities. The proposed method may lead to new developments in quantum metrology.
    Language English
    Publishing date 2020-07-29
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-020-69646-z
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  10. Article ; Online: Enophthalmos in silent brain syndrome.

    Lando, Leonardo / Paiva, Marina O S / Pereira, Luciano S

    Indian journal of ophthalmology

    2020  Volume 68, Issue 11, Page(s) 2536

    MeSH term(s) Brain ; Enophthalmos/diagnosis ; Enophthalmos/etiology ; Humans ; Maxillary Sinus ; Paranasal Sinus Diseases
    Language English
    Publishing date 2020-10-23
    Publishing country India
    Document type Journal Article
    ZDB-ID 187392-1
    ISSN 1998-3689 ; 0301-4738
    ISSN (online) 1998-3689
    ISSN 0301-4738
    DOI 10.4103/ijo.IJO_897_20
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