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Article ; Online: DNA Methylation-Based Assessment of Cell Composition in Human Pancreas and Islets.

Drawshy, Zeina / Neiman, Daniel / Fridlich, Ori / Peretz, Ayelet / Magenheim, Judith / Rozo, Andrea V / Doliba, Nicolai M / Stoffers, Doris A / Kaestner, Klaus H / Schatz, Desmond A / Wasserfall, Clive / Campbell-Thompson, Martha / Shapiro, James / Kaplan, Tommy / Shemer, Ruth / Glaser, Benjamin / Klochendler, Agnes / Dor, Yuval

Diabetes

2024 Volume 73, Issue 4, Page(s) 554–564

MeSH term(s)	Humans ; Diabetes Mellitus, Type 1/metabolism ; Diabetes Mellitus, Type 2/metabolism ; Islets of Langerhans/metabolism ; DNA Methylation ; Pancreas/metabolism ; Insulin/metabolism ; Insulin-Secreting Cells/metabolism ; Glucagon-Secreting Cells/metabolism
Chemical Substances	Insulin
Language	English
Publishing date	2024-01-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	80085-5
ISSN	1939-327X ; 0012-1797
ISSN (online)	1939-327X
ISSN	0012-1797
DOI	10.2337/db23-0704
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Article ; Online: Spliceosome-Associated microRNAs Signify Breast Cancer Cells and Portray Potential Novel Nuclear Targets.

Mahlab-Aviv, Shelly / Zohar, Keren / Cohen, Yael / Peretz, Ayelet R / Eliyahu, Tsiona / Linial, Michal / Sperling, Ruth

International journal of molecular sciences

2020 Volume 21, Issue 21

Abstract: MicroRNAs (miRNAs) act as negative regulators of gene expression in the cytoplasm. Previous studies have identified the presence of miRNAs in the nucleus. Here we study human breast cancer-derived cell-lines (MCF-7 and MDA-MB-231) and a non-tumorigenic ... ...

Abstract	MicroRNAs (miRNAs) act as negative regulators of gene expression in the cytoplasm. Previous studies have identified the presence of miRNAs in the nucleus. Here we study human breast cancer-derived cell-lines (MCF-7 and MDA-MB-231) and a non-tumorigenic cell-line (MCF-10A) and compare their miRNA sequences at the spliceosome fraction (SF). We report that the levels of miRNAs found in the spliceosome, their identity, and pre-miRNA segmental composition are cell-line specific. One such miRNA is miR-7704 whose genomic position overlaps HAGLR, a cancer-related lncRNA. We detected an inverse expression of miR-7704 and HAGLR in the tested cell lines. Specifically, inhibition of miR-7704 caused an increase in HAGLR expression. Furthermore, elevated levels of miR-7704 slightly altered the cell-cycle in MDA-MB-231. Altogether, we show that SF-miR-7704 acts as a tumor-suppressor gene with HAGLR being its nuclear target. The relative levels of miRNAs found in the spliceosome fractions (e.g., miR-100, miR-30a, and let-7 family) in non-tumorigenic relative to cancer-derived cell-lines was monitored. We found that the expression trend of the abundant miRNAs in SF was different from that reported in the literature and from the observation of large cohorts of breast cancer patients, suggesting that many SF-miRNAs act on targets that are different from the cytoplasmic ones. Altogether, we report on the potential of SF-miRNAs as an unexplored route for cancerous cell state.
MeSH term(s)	Apoptosis ; Biomarkers, Tumor/genetics ; Breast Neoplasms/genetics ; Breast Neoplasms/metabolism ; Breast Neoplasms/pathology ; Cell Proliferation ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; MicroRNAs/genetics ; RNA, Long Noncoding/genetics ; Spliceosomes/genetics ; Tumor Cells, Cultured
Chemical Substances	Biomarkers, Tumor ; MIRN7704 microRNA, human ; MicroRNAs ; RNA, Long Noncoding
Language	English
Publishing date	2020-10-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms21218132
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Article ; Online: Elevated cfDNA after exercise is derived primarily from mature polymorphonuclear neutrophils, with a minor contribution of cardiomyocytes.

Fridlich, Ori / Peretz, Ayelet / Fox-Fisher, Ilana / Pyanzin, Sheina / Dadon, Ziv / Shcolnik, Eilon / Sadeh, Ronen / Fialkoff, Gavriel / Sharkia, Israa / Moss, Joshua / Arpinati, Ludovica / Nice, Shachar / Nogiec, Christopher D / Ahuno, Samuel Terkper / Li, Rui / Taborda, Eddie / Dunkelbarger, Sonia / Fridlender, Zvi G / Polak, Paz /

Kaplan, Tommy / Friedman, Nir / Glaser, Benjamin / Shemer, Ruth / Constantini, Naama / Dor, Yuval

Cell reports. Medicine

2023 Volume 4, Issue 6, Page(s) 101074

Abstract: Strenuous physical exercise causes a massive elevation in the concentration of circulating cell-free DNA (cfDNA), which correlates with effort intensity and duration. The cellular sources and physiological drivers of this phenomenon are unknown. Using ... ...

Abstract	Strenuous physical exercise causes a massive elevation in the concentration of circulating cell-free DNA (cfDNA), which correlates with effort intensity and duration. The cellular sources and physiological drivers of this phenomenon are unknown. Using methylation patterns of cfDNA and associated histones, we show that cfDNA in exercise originates mostly in extramedullary polymorphonuclear neutrophils. Strikingly, cardiomyocyte cfDNA concentration increases after a marathon, consistent with elevated troponin levels and indicating low-level, delayed cardiac cell death. Physical impact, low oxygen levels, and elevated core body temperature contribute to neutrophil cfDNA release, while muscle contraction, increased heart rate, β-adrenergic signaling, or steroid treatment fail to cause elevation of cfDNA. Physical training reduces neutrophil cfDNA release after a standard exercise, revealing an inverse relationship between exercise-induced cfDNA release and training level. We speculate that the release of cfDNA from neutrophils in exercise relates to the activation of neutrophils in the context of exercise-induced muscle damage.
MeSH term(s)	Neutrophils ; Myocytes, Cardiac ; Cell-Free Nucleic Acids ; Exercise/physiology ; Histones
Chemical Substances	Cell-Free Nucleic Acids ; Histones
Language	English
Publishing date	2023-06-07
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2666-3791
ISSN (online)	2666-3791
DOI	10.1016/j.xcrm.2023.101074
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Article ; Online: Small RNA sequences derived from pre-microRNAs in the supraspliceosome.

Mahlab-Aviv, Shelly / Boulos, Ayub / Peretz, Ayelet R / Eliyahu, Tsiona / Carmel, Liran / Sperling, Ruth / Linial, Michal

Nucleic acids research

2018 Volume 46, Issue 20, Page(s) 11014–11029

Abstract: MicroRNAs (miRNAs) are short non-coding RNAs that negatively regulate the expression and translation of genes in healthy and diseased tissues. Herein, we characterize short RNAs from human HeLa cells found in the supraspliceosome, a nuclear dynamic ... ...

Abstract	MicroRNAs (miRNAs) are short non-coding RNAs that negatively regulate the expression and translation of genes in healthy and diseased tissues. Herein, we characterize short RNAs from human HeLa cells found in the supraspliceosome, a nuclear dynamic machine in which pre-mRNA processing occurs. We sequenced small RNAs (<200 nt) extracted from the supraspliceosome, and identified sequences that are derived from 200 miRNAs genes. About three quarters of them are mature miRNAs, whereas the rest account for various defined regions of the pre-miRNA, and its hairpin-loop precursor. Out of these aligned sequences, 53 were undetected in cellular extract, and the abundance of additional 48 strongly differed from that in cellular extract. Notably, we describe seven abundant miRNA-derived sequences that overlap non-coding exons of their host gene. The rich collection of sequences identical to pre-miRNAs at the supraspliceosome suggests overlooked nuclear functions. Specifically, the abundant hsa-mir-99b may affect splicing of LINC01129 primary transcript through base-pairing with its exon-intron junction. Using suppression and overexpression experiments, we show that hsa-mir-7704 negatively regulates the level of the lncRNA HAGLR. We claim that in cases of extended base-pairing complementarity, such supraspliceosomal pre-miRNA sequences might have a role in transcription attenuation, maturation and processing.
MeSH term(s)	Base Sequence ; Cell Line ; Gene Expression Regulation ; HeLa Cells ; High-Throughput Nucleotide Sequencing ; Humans ; MicroRNAs/genetics ; MicroRNAs/metabolism ; RNA Precursors/genetics ; RNA Processing, Post-Transcriptional ; RNA Splicing ; Spliceosomes/genetics ; Spliceosomes/metabolism
Chemical Substances	MicroRNAs ; RNA Precursors
Language	English
Publishing date	2018-09-10
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	186809-3
ISSN	1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
ISSN (online)	1362-4962 ; 1362-4954
ISSN	0301-5610 ; 0305-1048
DOI	10.1093/nar/gky791
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Article ; Online: Lessons from applied large-scale pooling of 133,816 SARS-CoV-2 RT-PCR tests.

Barak, Netta / Ben-Ami, Roni / Sido, Tal / Perri, Amir / Shtoyer, Aviad / Rivkin, Mila / Licht, Tamar / Peretz, Ayelet / Magenheim, Judith / Fogel, Irit / Livneh, Ayalah / Daitch, Yutti / Oiknine-Djian, Esther / Benedek, Gil / Dor, Yuval / Wolf, Dana G / Yassour, Moran

Science translational medicine

2021 Volume 13, Issue 589

Abstract: Pooling multiple swab samples before RNA extraction and real-time reverse transcription polymerase chain reaction (RT-PCR) analysis has been proposed as a strategy to reduce costs and increase throughput of severe acute respiratory syndrome coronavirus 2 ...

Abstract	Pooling multiple swab samples before RNA extraction and real-time reverse transcription polymerase chain reaction (RT-PCR) analysis has been proposed as a strategy to reduce costs and increase throughput of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) tests. However, reports on practical large-scale group testing for SARS-CoV-2 have been scant. Key open questions concern reduced sensitivity due to sample dilution, the rate of false positives, the actual efficiency (number of tests saved by pooling), and the impact of infection rate in the population on assay performance. Here, we report an analysis of 133,816 samples collected between April and September 2020 and tested by Dorfman pooling for the presence of SARS-CoV-2. We spared 76% of RNA extraction and RT-PCR tests, despite the frequently changing prevalence (0.5 to 6%). We observed pooling efficiency and sensitivity that exceeded theoretical predictions, which resulted from the nonrandom distribution of positive samples in pools. Overall, our findings support the use of pooling for efficient large-scale SARS-CoV-2 testing.
MeSH term(s)	COVID-19 ; COVID-19 Testing ; Humans ; RNA, Viral/genetics ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2 ; Sensitivity and Specificity ; Specimen Handling
Chemical Substances	RNA, Viral
Language	English
Publishing date	2021-02-22
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2518854-9
ISSN	1946-6242 ; 1946-6234
ISSN (online)	1946-6242
ISSN	1946-6234
DOI	10.1126/scitranslmed.abf2823
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Article ; Online: The DNA methylome of human vascular endothelium and its use in liquid biopsies.

Peretz, Ayelet / Loyfer, Netanel / Piyanzin, Sheina / Ochana, Bracha Lea / Neiman, Daniel / Magenheim, Judith / Klochendler, Agnes / Drawshy, Zeina / Fox-Fisher, Ilana / Fridlich, Ori / Moss, Joshua / Cohen, Daniel / Zemmour, Hai / Cann, Gordon / Bredno, Joerg / Venn, Oliver / Avni, Batia / Alekberli, Tural / Samet, Yaacov /

Korach, Amit / Wald, Ori / Yutkin, Vladimir / Izhar, Uzi / Pillar, Nir / Grompe, Markus / Fridlender, Zvi / Rokach, Ariel / Planer, David / Landesberg, Giora / Glaser, Benjamin / Shemer, Ruth / Kaplan, Tommy / Dor, Yuval

Med (New York, N.Y.)

2023 Volume 4, Issue 4, Page(s) 263–281.e4

Abstract: Background: Vascular endothelial cells (VECs) are an essential component of each tissue, contribute to multiple pathologies, and are targeted by important drugs. Yet, there is a shortage of biomarkers to assess VEC turnover.: Methods: To develop DNA ... ...

Abstract	Background: Vascular endothelial cells (VECs) are an essential component of each tissue, contribute to multiple pathologies, and are targeted by important drugs. Yet, there is a shortage of biomarkers to assess VEC turnover. Methods: To develop DNA methylation-based liquid biopsies for VECs, we determined the methylome of VECs isolated from freshly dissociated human tissues. Findings: A comparison with a human cell-type methylome atlas yielded thousands of loci that are uniquely unmethylated in VECs. These sites are typically gene enhancers, often residing adjacent to VEC-specific genes. We also identified hundreds of genomic loci that are differentially methylated in organotypic VECs, indicating that VECs feeding specific organs are distinct cell types with a stable epigenetic identity. We established universal and lung-specific VEC markers and evaluated their presence in circulating cell-free DNA (cfDNA). Nearly 2.5% of cfDNA in the plasma of healthy individuals originates from VECs. Sepsis, graft versus host disease, and cardiac catheterization are associated with elevated levels of VEC-derived cfDNA, indicative of vascular damage. Lung-specific VEC cfDNA is selectively elevated in patients with chronic obstructive pulmonary disease (COPD) or lung cancer, revealing tissue-specific vascular turnover. Conclusions: VEC cfDNA biomarkers inform vascular dynamics in health and disease, potentially contributing to early diagnosis and monitoring of pathologies, and assessment of drug activity. Funding: This work was supported by the Beutler Research Program, Helmsley Charitable Trust, JDRF, Grail and the DON Foundation (to Y.D.). Y.D holds the Walter & Greta Stiel Chair in heart studies. B.G., R.S., J.M., D.N., T.K., and Y.D. filed patents on cfDNA analysis.
MeSH term(s)	Humans ; Epigenome ; Endothelium, Vascular ; Endothelial Cells/metabolism ; Biomarkers/metabolism ; Cell-Free Nucleic Acids ; Liquid Biopsy
Chemical Substances	Biomarkers ; Cell-Free Nucleic Acids
Language	English
Publishing date	2023-04-14
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ISSN	2666-6340
ISSN (online)	2666-6340
DOI	10.1016/j.medj.2023.03.006
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Article ; Online: Remote immune processes revealed by immune-derived circulating cell-free DNA.

eLife

2021 Volume 10

Abstract: Blood cell counts often fail to report on immune processes occurring in remote tissues. Here, we use immune cell type-specific methylation patterns in circulating cell-free DNA (cfDNA) for studying human immune cell dynamics. We characterized cfDNA ... ...

Abstract	Blood cell counts often fail to report on immune processes occurring in remote tissues. Here, we use immune cell type-specific methylation patterns in circulating cell-free DNA (cfDNA) for studying human immune cell dynamics. We characterized cfDNA released from specific immune cell types in healthy individuals (N = 242), cross sectionally and longitudinally. Immune cfDNA levels had no individual steady state as opposed to blood cell counts, suggesting that cfDNA concentration reflects adjustment of cell survival to maintain homeostatic cell numbers. We also observed selective elevation of immune-derived cfDNA upon perturbations of immune homeostasis. Following influenza vaccination (N = 92), B-cell-derived cfDNA levels increased prior to elevated B-cell counts and predicted efficacy of antibody production. Patients with eosinophilic esophagitis (N = 21) and B-cell lymphoma (N = 27) showed selective elevation of eosinophil and B-cell cfDNA, respectively, which were undetectable by cell counts in blood. Immune-derived cfDNA provides a novel biomarker for monitoring immune responses to physiological and pathological processes that are not accessible using conventional methods.
MeSH term(s)	Adult ; Aged ; Biomarkers, Tumor/metabolism ; Cell-Free Nucleic Acids/metabolism ; DNA Methylation ; Female ; Humans ; Immunity ; Male ; Middle Aged ; Young Adult
Chemical Substances	Biomarkers, Tumor ; Cell-Free Nucleic Acids
Language	English
Publishing date	2021-11-29
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2687154-3
ISSN	2050-084X ; 2050-084X
ISSN (online)	2050-084X
ISSN	2050-084X
DOI	10.7554/eLife.70520
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Article ; Online: Universal lung epithelium DNA methylation markers for detection of lung damage in liquid biopsies.

Magenheim, Judith / Rokach, Ariel / Peretz, Ayelet / Loyfer, Netanel / Cann, Gordon / Amini, Hamed / Moradi, Patriss / Nagaraju, Sudharani / Sameer, Wafa / Cohen, Assaf / Fogel, Ophir / Kuint, Rottem / Abutbul, Avraham / Abu Rmeileh, Aiman / Karameh, Mutaz / Cohen Goichman, Polina / Wald, Ori / Korach, Amit / Neiman, Daniel /

Fox-Fisher, Ilana / Moss, Joshua / Cohen, Daniel / Piyanzin, Sheina / Ben Ami, Roni / Quteineh, Ahmad / Golomb, Eliahu / Shemer, Ruth / Glaser, Benjamin / Kaplan, Tommy / Fridlender, Zvi G / Dor, Yuval

The European respiratory journal

2022 Volume 60, Issue 5

Abstract: Background: Circulating biomarkers for lung damage are lacking. Lung epithelium-specific DNA methylation patterns can potentially report the presence of lung-derived cell-free DNA (cfDNA) in blood, as an indication of lung cell death.: Methods: We ... ...

Abstract	Background: Circulating biomarkers for lung damage are lacking. Lung epithelium-specific DNA methylation patterns can potentially report the presence of lung-derived cell-free DNA (cfDNA) in blood, as an indication of lung cell death. Methods: We sorted human lung alveolar and bronchial epithelial cells from surgical specimens, and obtained their methylomes using whole-genome bisulfite sequencing. We developed a PCR sequencing assay determining the methylation status of 17 loci with lung-specific methylation patterns, and used it to assess lung-derived cfDNA in the plasma of healthy volunteers and patients with lung disease. Results: Loci that are uniquely unmethylated in alveolar or bronchial epithelial cells are enriched for enhancers controlling lung-specific genes. Methylation markers extracted from these methylomes revealed that normal lung cell turnover probably releases cfDNA into the air spaces, rather than to blood. People with advanced lung cancer show a massive elevation of lung cfDNA concentration in blood. Among individuals undergoing bronchoscopy, lung-derived cfDNA is observed in the plasma of those later diagnosed with lung cancer, and to a lesser extent in those diagnosed with other lung diseases. Lung cfDNA is also elevated in patients with acute exacerbation of COPD compared with patients with stable disease, and is associated with future exacerbation and mortality in these patients. Conclusions: Universal cfDNA methylation markers of normal lung epithelium allow for mutation-independent, sensitive and specific detection of lung-derived cfDNA, reporting on ongoing lung injury. Such markers can find broad utility in the study of normal and pathologic human lung dynamics.
MeSH term(s)	Humans ; DNA Methylation ; Cell-Free Nucleic Acids/genetics ; Liquid Biopsy ; Biomarkers ; Epithelium ; Lung ; Lung Neoplasms/genetics ; Biomarkers, Tumor/genetics
Chemical Substances	Cell-Free Nucleic Acids ; Biomarkers ; Biomarkers, Tumor
Language	English
Publishing date	2022-11-03
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639359-7
ISSN	1399-3003 ; 0903-1936
ISSN (online)	1399-3003
ISSN	0903-1936
DOI	10.1183/13993003.03056-2021
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Article ; Online: B cell-derived cfDNA after primary BNT162b2 mRNA vaccination anticipates memory B cells and SARS-CoV-2 neutralizing antibodies.

Fox-Fisher, Ilana / Piyanzin, Sheina / Briller, Mayan / Oiknine-Djian, Esther / Alfi, Or / Ben-Ami, Roni / Peretz, Ayelet / Neiman, Daniel / Ochana, Bracha-Lea / Fridlich, Ori / Drawshy, Zeina / Klochendler, Agnes / Magenheim, Judith / Share, Danielle / Avrahami, Ran / Ribak, Yaarit / Talmon, Aviv / Rubin, Limor / Milman, Neta /

Segev, Meital / Feldman, Erik / Tal, Yuval / Shen-Orr, Shai S / Glaser, Benjamin / Shemer, Ruth / Wolf, Dana / Dor, Yuval

Med (New York, N.Y.)

2022 Volume 3, Issue 7, Page(s) 468–480.e5

Abstract: Background: Much remains unknown regarding the response of the immune system to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) vaccination.: Methods: We employed circulating cell-free DNA (cfDNA) to assess the turnover of specific ... ...

Abstract	Background: Much remains unknown regarding the response of the immune system to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) vaccination. Methods: We employed circulating cell-free DNA (cfDNA) to assess the turnover of specific immune cell types following administration of the Pfizer/BioNTech vaccine. Findings: The levels of B cell cfDNA after the primary dose correlated with development of neutralizing antibodies and memory B cells after the booster, revealing a link between early B cell turnover-potentially reflecting affinity maturation-and later development of effective humoral response. We also observed co-elevation of B cell, T cell, and monocyte cfDNA after the booster, underscoring the involvement of innate immune cell turnover in the development of humoral and cellular adaptive immunity. Actual cell counts remained largely stable following vaccination, other than a previously demonstrated temporary reduction in neutrophil and lymphocyte counts. Conclusions: Immune cfDNA dynamics reveal the crucial role of the primary SARS-CoV-2 vaccine in shaping responses of the immune system following the booster vaccine. Funding: This work was supported by a generous gift from Shlomo Kramer. Supported by grants from Human Islet Research Network (HIRN UC4DK116274 and UC4DK104216 to R.S. and Y.D.), Ernest and Bonnie Beutler Research Program of Excellence in Genomic Medicine, The Alex U Soyka Pancreatic Cancer Fund, The Israel Science Foundation, the Waldholtz/Pakula family, the Robert M. and Marilyn Sternberg Family Charitable Foundation, the Helmsley Charitable Trust, Grail, and the DON Foundation (to Y.D.). Y.D. holds the Walter and Greta Stiel Chair and Research Grant in Heart Studies. I.F.-F. received a fellowship from the Glassman Hebrew University Diabetes Center.
MeSH term(s)	Adult ; Aged ; Antibodies, Neutralizing/genetics ; Antibodies, Neutralizing/immunology ; Antibodies, Viral/genetics ; Antibodies, Viral/immunology ; BNT162 Vaccine/administration & dosage ; COVID-19/immunology ; COVID-19/prevention & control ; Cell-Free Nucleic Acids/genetics ; Cell-Free Nucleic Acids/immunology ; Female ; Humans ; Immunization, Secondary ; Male ; Memory B Cells/immunology ; Memory B Cells/metabolism ; Middle Aged ; SARS-CoV-2/immunology ; Young Adult
Chemical Substances	Antibodies, Neutralizing ; Antibodies, Viral ; Cell-Free Nucleic Acids ; BNT162 Vaccine (N38TVC63NU)
Language	English
Publishing date	2022-05-19
Publishing country	United States
Document type	Journal Article
ISSN	2666-6340
ISSN (online)	2666-6340
DOI	10.1016/j.medj.2022.05.005
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Article ; Online: A DNA methylation atlas of normal human cell types.

Loyfer, Netanel / Magenheim, Judith / Peretz, Ayelet / Cann, Gordon / Bredno, Joerg / Klochendler, Agnes / Fox-Fisher, Ilana / Shabi-Porat, Sapir / Hecht, Merav / Pelet, Tsuria / Moss, Joshua / Drawshy, Zeina / Amini, Hamed / Moradi, Patriss / Nagaraju, Sudharani / Bauman, Dvora / Shveiky, David / Porat, Shay / Dior, Uri /

Rivkin, Gurion / Or, Omer / Hirshoren, Nir / Carmon, Einat / Pikarsky, Alon / Khalaileh, Abed / Zamir, Gideon / Grinbaum, Ronit / Abu Gazala, Machmud / Mizrahi, Ido / Shussman, Noam / Korach, Amit / Wald, Ori / Izhar, Uzi / Erez, Eldad / Yutkin, Vladimir / Samet, Yaacov / Rotnemer Golinkin, Devorah / Spalding, Kirsty L / Druid, Henrik / Arner, Peter / Shapiro, A M James / Grompe, Markus / Aravanis, Alex / Venn, Oliver / Jamshidi, Arash / Shemer, Ruth / Dor, Yuval / Glaser, Benjamin / Kaplan, Tommy

Nature

2023 Volume 613, Issue 7943, Page(s) 355–364

Abstract: DNA methylation is a fundamental epigenetic mark that governs gene expression and chromatin organization, thus providing a window into cellular identity and developmental ... ...

Abstract	DNA methylation is a fundamental epigenetic mark that governs gene expression and chromatin organization, thus providing a window into cellular identity and developmental processes
MeSH term(s)	Humans ; Cell Line ; Cells/classification ; Cells/metabolism ; Chromatin/genetics ; Chromatin/metabolism ; CpG Islands/genetics ; DNA/genetics ; DNA/metabolism ; DNA Methylation ; Embryonic Development ; Enhancer Elements, Genetic ; Epigenesis, Genetic ; Epigenome ; Organ Specificity ; Polycomb-Group Proteins/metabolism ; Whole Genome Sequencing
Chemical Substances	Chromatin ; CTCF protein, human ; DNA (9007-49-2) ; hydrogen sulfite (OJ9787WBLU) ; Polycomb-Group Proteins
Language	English
Publishing date	2023-01-04
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	120714-3
ISSN	1476-4687 ; 0028-0836
ISSN (online)	1476-4687
ISSN	0028-0836
DOI	10.1038/s41586-022-05580-6
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