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  1. AU="Perez Prados, Graciela"
  2. AU="Maghraby, Yasmin R"

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  1. Article ; Online: Vitamin D deficiency among patients with pulmonary hypertension.

    Atamañuk, Andrés N / Litewka, Diego F / Baratta, Sergio J / Seropian, Ignacio M / Perez Prados, Graciela / Payaslian, Miguel O / Ortiz Fragola, Juan P / Escribano Subias, Pilar

    BMC pulmonary medicine

    2019  Volume 19, Issue 1, Page(s) 258

    Abstract: Background: There is little information about vitamin D (Vit D) deficiency in patients with pulmonary hypertension (PH). The objective of this study was: 1) compare Vit D levels between patients with PH, left ventricular failure (LVF) and healthy ... ...

    Abstract Background: There is little information about vitamin D (Vit D) deficiency in patients with pulmonary hypertension (PH). The objective of this study was: 1) compare Vit D levels between patients with PH, left ventricular failure (LVF) and healthy subjects (HS); 2) correlate, in patients with PH, Vit D levels with prognosis-related variables, such as the 6-min walk test (6MWT).
    Methods: Vitamin D levels were measured in a cross-sectional study in 126 patients from one of three groups: patients with PH (n = 53), patients with LVF (n = 42) and healthy subjects (n = 31). In all groups, 8-h fasting blood samples were obtained in the morning. In the PH and the LVF group, functional class (WHO criteria), metres covered in the 6MWT and echocardiographic parameters were analysed. In the PH group, plasma N terminal pro B type natriuretic peptide (NT-proBNP) level was analysed and a complete haemodynamic evaluation by right heart catheterisation was made.
    Results: Mean Vit D levels were lower in PH than in both other groups (ng/ml, mean ± SD): PH 19.25 ± 10, LVF 25.68 ± 12, HS 28.8 ± 12 (PH vs LVF p = 0.017, PH vs HS p = 0.001 and HS vs LVF p = 0.46). Vit D deficiency prevalence was higher in PH as compared to the other groups (PH 53.8%, LVF 45.2%, HS 25%, p = 0.01). Patients with PH in functional class (FC; WHO criteria) III-IV had higher Vit D deficiency prevalence than those in FC I-II (86.7% vs 40.5%, p = 0.003). There was a significant linear correlation between the 6MWT and Vit D levels in PH (p < 0.01), but not in LVF (p = 0.69).
    Conclusions: Vit D levels were lower in patients with PH as compared to patients with LVF and HS and correlated directly with 6-min walk distance.
    MeSH term(s) Adult ; Aged ; Case-Control Studies ; Cross-Sectional Studies ; Echocardiography ; Female ; Heart Failure/epidemiology ; Humans ; Hypertension, Pulmonary/blood ; Hypertension, Pulmonary/epidemiology ; Hypertension, Pulmonary/physiopathology ; Male ; Middle Aged ; Natriuretic Peptide, Brain/blood ; Peptide Fragments/blood ; Prevalence ; Prognosis ; Ventricular Dysfunction, Left/epidemiology ; Vitamin D/analogs & derivatives ; Vitamin D/blood ; Vitamin D Deficiency/blood ; Vitamin D Deficiency/epidemiology ; Walk Test ; Young Adult
    Chemical Substances Peptide Fragments ; pro-brain natriuretic peptide (1-76) ; Natriuretic Peptide, Brain (114471-18-0) ; Vitamin D (1406-16-2) ; 25-hydroxyvitamin D (A288AR3C9H)
    Language English
    Publishing date 2019-12-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 2059871-3
    ISSN 1471-2466 ; 1471-2466
    ISSN (online) 1471-2466
    ISSN 1471-2466
    DOI 10.1186/s12890-019-1011-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Cytokine production but lack of proliferation in peripheral blood mononuclear cells from chronic Chagas' disease cardiomyopathy patients in response to T. cruzi ribosomal P proteins.

    Longhi, Silvia A / Atienza, Augusto / Perez Prados, Graciela / Buying, Alcinette / Balouz, Virginia / Buscaglia, Carlos A / Santos, Radleigh / Tasso, Laura M / Bonato, Ricardo / Chiale, Pablo / Pinilla, Clemencia / Judkowski, Valeria A / Gómez, Karina A

    PLoS neglected tropical diseases

    2014  Volume 8, Issue 6, Page(s) e2906

    Abstract: Background: Trypanosoma cruzi ribosomal P proteins, P2β and P0, induce high levels of antibodies in patients with chronic Chagas' disease Cardiomyopathy (CCC). It is well known that these antibodies alter the beating rate of cardiomyocytes and provoke ... ...

    Abstract Background: Trypanosoma cruzi ribosomal P proteins, P2β and P0, induce high levels of antibodies in patients with chronic Chagas' disease Cardiomyopathy (CCC). It is well known that these antibodies alter the beating rate of cardiomyocytes and provoke apoptosis by their interaction with β1-adrenergic and M2-muscarinic cardiac receptors. Based on these findings, we decided to study the cellular immune response to these proteins in CCC patients compared to non-infected individuals.
    Methodology/principal findings: We evaluated proliferation, presence of surface activation markers and cytokine production in peripheral blood mononuclear cells (PBMC) stimulated with P2β, the C-terminal portion of P0 (CP0) proteins and T. cruzi lysate from CCC patients predominantly infected with TcVI lineage. PBMC from CCC patients cultured with P2β or CP0 proteins, failed to proliferate and express CD25 and HLA-DR on T cell populations. However, multiplex cytokine assays showed that these antigens triggered higher secretion of IL-10, TNF-α and GM-CSF by PBMC as well as both CD4+ and CD8+ T cells subsets of CCC subjects. Upon T. cruzi lysate stimulation, PBMC from CCC patients not only proliferated but also became activated within the context of Th1 response. Interestingly, T. cruzi lysate was also able to induce the secretion of GM-CSF by CD4+ or CD8+ T cells.
    Conclusions/significance: Our results showed that although the lack of PBMC proliferation in CCC patients in response to ribosomal P proteins, the detection of IL-10, TNF-α and GM-CSF suggests that specific T cells could have both immunoregulatory and pro-inflammatory potential, which might modulate the immune response in Chagas' disease. Furthermore, it was possible to demonstrate for the first time that GM-CSF was produced by PBMC of CCC patients in response not only to recombinant ribosomal P proteins but also to parasite lysate, suggesting the value of this cytokine to evaluate T cells responses in T. cruzi infection.
    MeSH term(s) Adult ; Aged ; Cell Proliferation ; Cells, Cultured ; Chagas Cardiomyopathy/pathology ; Cytokines/metabolism ; Female ; Humans ; Leukocytes, Mononuclear/immunology ; Lymphocyte Activation ; Lymphocyte Subsets/immunology ; Male ; Middle Aged ; Phosphoproteins/immunology ; Protozoan Proteins/immunology ; Ribosomal Proteins/immunology ; Trypanosoma cruzi/immunology
    Chemical Substances Cytokines ; Phosphoproteins ; Protozoan Proteins ; Ribosomal Proteins ; phosphoprotein P2, ribosomal ; ribosomal PO protein, Trypanosoma cruzi
    Language English
    Publishing date 2014-06-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2429704-5
    ISSN 1935-2735 ; 1935-2727
    ISSN (online) 1935-2735
    ISSN 1935-2727
    DOI 10.1371/journal.pntd.0002906
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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