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Article: Characterization of the Bacterial Communities Inhabiting Tropical Propolis of Puerto Rico.

Pérez Matos, Ana E / Bacci, Giovanni / Borruso, Luigimaria / Landolfi, Maria / Petrocchi, Dominique / Renzi, Sonia / Perito, Brunella

Microorganisms

2023 Volume 11, Issue 5

Abstract: Propolis is a resinous material produced by honeybees from different plant sources and used in the hive as a building material and to protect the colony from parasites and pathogens. Despite its antimicrobial properties, recent studies showed that ... ...

Abstract	Propolis is a resinous material produced by honeybees from different plant sources and used in the hive as a building material and to protect the colony from parasites and pathogens. Despite its antimicrobial properties, recent studies showed that propolis hosts diverse microbial strains, some with great antimicrobial potential. In this study, the first description of the bacterial community of propolis produced by the gentle Africanized honeybee was reported. Propolis was sampled from hives of two different geographic areas of Puerto Rico (PR, USA), and the associated microbiota investigated by both cultivation and metataxonomic approaches. Metabarcoding analysis showed appreciable bacterial diversity in both areas and statistically significant dissimilarity in the taxa composition of the two areas, probably due to the different climatic conditions. Both metabarcoding and cultivation data revealed the presence of taxa already detected in other hive components and compatible with the bee's foraging environment. Isolated bacteria and propolis extracts showed antimicrobial activity against Gram-positive and Gram-negative bacterial tester strains. These results support the hypothesis that the propolis microbiota could contribute to propolis' antimicrobial properties.
Language	English
Publishing date	2023-04-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms11051130
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Article ; Online: ECG-only explainable deep learning algorithm predicts the risk for malignant ventricular arrhythmia in phospholamban cardiomyopathy.

van de Leur, Rutger R / de Brouwer, Remco / Bleijendaal, Hidde / Verstraelen, Tom E / Mahmoud, Belend / Perez-Matos, Ana / Dickhoff, Cathelijne / Schoonderwoerd, Bas A / Germans, Tjeerd / Houweling, Arjan / van der Zwaag, Paul A / Cox, Moniek G P J / Peter van Tintelen, J / Te Riele, Anneline S J M / van den Berg, Maarten P / Wilde, Arthur A M / Doevendans, Pieter A / de Boer, Rudolf A / van Es, René

Heart rhythm

2024

Abstract: Background: Phospholamban (PLN) p.(Arg14del) variant carriers are at risk for development of malignant ventricular arrhythmia (MVA). Accurate risk stratification allows timely implantation of intracardiac defibrillators and is currently performed with a ...

Abstract	Background: Phospholamban (PLN) p.(Arg14del) variant carriers are at risk for development of malignant ventricular arrhythmia (MVA). Accurate risk stratification allows timely implantation of intracardiac defibrillators and is currently performed with a multimodality prediction model. Objective: This study aimed to investigate whether an explainable deep learning-based approach allows risk prediction with only electrocardiogram (ECG) data. Methods: A total of 679 PLN p.(Arg14del) carriers without MVA at baseline were identified. A deep learning-based variational auto-encoder, trained on 1.1 million ECGs, was used to convert the 12-lead baseline ECG into its FactorECG, a compressed version of the ECG that summarizes it into 32 explainable factors. Prediction models were developed by Cox regression. Results: The deep learning-based ECG-only approach was able to predict MVA with a C statistic of 0.79 (95% CI, 0.76-0.83), comparable to the current prediction model (C statistic, 0.83 [95% CI, 0.79-0.88]; P = .054) and outperforming a model based on conventional ECG parameters (low-voltage ECG and negative T waves; C statistic, 0.65 [95% CI, 0.58-0.73]; P < .001). Clinical simulations showed that a 2-step approach, with ECG-only screening followed by a full workup, resulted in 60% less additional diagnostics while outperforming the multimodal prediction model in all patients. A visualization tool was created to provide interactive visualizations (https://pln.ecgx.ai). Conclusion: Our deep learning-based algorithm based on ECG data only accurately predicts the occurrence of MVA in PLN p.(Arg14del) carriers, enabling more efficient stratification of patients who need additional diagnostic testing and follow-up.
Language	English
Publishing date	2024-02-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	2229357-7
ISSN	1556-3871 ; 1547-5271
ISSN (online)	1556-3871
ISSN	1547-5271
DOI	10.1016/j.hrthm.2024.02.038
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Article: Bacterial diversity associated with the Caribbean tunicate Ecteinascidia turbinata.

Pérez-Matos, Ana E / Rosado, William / Govind, Nadathur S

Antonie van Leeuwenhoek

2007 Volume 92, Issue 2, Page(s) 155–164

Abstract: The Caribbean tunicate, Ecteinascidia turbinata produces the anti-cancer agent ET-743 that could well be a metabolite of an associated bacterial strain. This current study aims at the analysis of bacteria that are persistently and specifically associated ...

Abstract	The Caribbean tunicate, Ecteinascidia turbinata produces the anti-cancer agent ET-743 that could well be a metabolite of an associated bacterial strain. This current study aims at the analysis of bacteria that are persistently and specifically associated with this invertebrate. Utilizing techniques such as denaturing gradient gel electrophoresis, DNA sequencing and phylogenetic analysis of bacteria from E. turbinata collected from different locations in the Caribbean Sea, we report here the identification of five possible persistently associated bacteria. Of these, only one organism, Candidatus Endoecteinascidia frumentensis, was found specifically associated to E. turbinata from the Caribbean and has also been found to be associated with E. turbinata from the Mediterranean. These experiments suggest that assessment of bacterial diversity associated with invertebrates from different geographical sites might be an effective way of identifying persistently and specifically associated bacteria.
MeSH term(s)	Animals ; Bacteria/genetics ; Bacteria/isolation & purification ; Caribbean Region ; DNA, Bacterial/genetics ; DNA, Ribosomal/genetics ; Genetic Variation ; Molecular Sequence Data ; Phylogeny ; Urochordata/microbiology
Chemical Substances	DNA, Bacterial ; DNA, Ribosomal
Language	English
Publishing date	2007-08
Publishing country	Netherlands
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	214861-4
ISSN	1572-9699 ; 0003-6072
ISSN (online)	1572-9699
ISSN	0003-6072
DOI	10.1007/s10482-007-9143-9
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Article ; Online: Embolization of a left atrial appendage closure device unmasked by intermittent left bundle branch block.

Pérez Matos, Ana J / Swaans, Martin J / Rensing, Benno J W M / Heijmen, Robin H / Mast, E Gijs / Boersma, Lucas V A / Post, Martijn C

JACC. Cardiovascular interventions

2014 Volume 7, Issue 9, Page(s) e115–7

MeSH term(s)	Aged, 80 and over ; Atrial Appendage/physiopathology ; Atrial Fibrillation/diagnosis ; Atrial Fibrillation/physiopathology ; Atrial Fibrillation/therapy ; Bundle-Branch Block/diagnosis ; Bundle-Branch Block/etiology ; Bundle-Branch Block/physiopathology ; Bundle-Branch Block/therapy ; Cardiac Catheterization/adverse effects ; Cardiac Catheterization/instrumentation ; Device Removal ; Echocardiography, Three-Dimensional ; Echocardiography, Transesophageal ; Electrocardiography ; Embolism/diagnosis ; Embolism/etiology ; Embolism/physiopathology ; Embolism/therapy ; Equipment Design ; Foreign-Body Migration/diagnosis ; Foreign-Body Migration/etiology ; Foreign-Body Migration/physiopathology ; Foreign-Body Migration/therapy ; Humans ; Male ; Treatment Outcome
Language	English
Publishing date	2014-09
Publishing country	United States
Document type	Case Reports ; Journal Article ; Video-Audio Media
ZDB-ID	2452157-7
ISSN	1876-7605 ; 1936-8798
ISSN (online)	1876-7605
ISSN	1936-8798
DOI	10.1016/j.jcin.2014.01.177
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Article: Bacterial diversity associated with the Caribbean tunicate Ecteinascidia turbinata

Pérez-Matos, Ana E / Rosado, William / Govind, Nadathur S

Antonie van Leeuwenhoek. 2007 Aug., v. 92, no. 2

2007

Abstract	The Caribbean tunicate, Ecteinascidia turbinata produces the anti-cancer agent ET-743 that could well be a metabolite of an associated bacterial strain. This current study aims at the analysis of bacteria that are persistently and specifically associated with this invertebrate. Utilizing techniques such as denaturing gradient gel electrophoresis, DNA sequencing and phylogenetic analysis of bacteria from E. turbinata collected from different locations in the Caribbean Sea, we report here the identification of five possible persistently associated bacteria. Of these, only one organism, Candidatus Endoecteinascidia frumentensis, was found specifically associated to E. turbinata from the Caribbean and has also been found to be associated with E. turbinata from the Mediterranean. These experiments suggest that assessment of bacterial diversity associated with invertebrates from different geographical sites might be an effective way of identifying persistently and specifically associated bacteria.
Keywords	bacteria ; denaturing gradient gel electrophoresis ; invertebrates ; phylogeny ; sequence analysis ; Caribbean ; Caribbean Sea
Language	English
Dates of publication	2007-08
Size	p. 155-164.
Publisher	Kluwer Academic Publishers
Publishing place	Dordrecht
Document type	Article
ZDB-ID	214861-4
ISSN	1572-9699 ; 0003-6072
ISSN (online)	1572-9699
ISSN	0003-6072
DOI	10.1007/s10482-007-9143-9
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Article ; Online: Clinical Characteristics of Patients Undergoing Right Heart Catheterizations in Community Hospitals.

Jansen, Samara M A / Huis In 't Veld, Anna E / Tolen, Peter Hans C G / Jacobs, Wouter / Willemsen, H M / Grotjohan, Hans P / Waskowsky, Marc / van der Maten, Jan / van der Weerdt, Arno / Hoekstra, Romke / Pérez Matos, Ana J / Overbeek, Maria J / Mollema, Sjoerd A / El Bouazzaoui, Lahssan H Hassan / Vriend, Joris W J / Roorda, J Milena M / de Nooijer, Ramon / van der Lee, Ivo / Voogel, A J /

Post, Johannes C / Macken, Thomas / Aerts, Jacqueline M / van de Ven, Marjo J T / Bergman, Heidi / Bakker-de Boo, Mirjam / de Boer, Roline C / Vonk Noordegraaf, Anton / de Man, Frances S / Bogaard, Harm Jan

Journal of the American Heart Association

2022 Volume 11, Issue 17, Page(s) e025143

Abstract: Background Recognition of precapillary pulmonary hypertension (PH) has significant implications for patient management. However, the low a priori chance to find this rare condition in community hospitals may create a barrier against performing a right ... ...

Abstract	Background Recognition of precapillary pulmonary hypertension (PH) has significant implications for patient management. However, the low a priori chance to find this rare condition in community hospitals may create a barrier against performing a right heart catheterization (RHC). This could result in misclassification of PH and delayed diagnosis/treatment of precapillary PH. Therefore, we investigated patient characteristics and echocardiographic parameters associated with the decision whether to perform an RHC in patients with incident PH in 12 Dutch community hospitals. Methods and Results In total, 275 patients were included from the OPTICS (Optimizing PH Diagnostic Network in Community Hospitals) registry, a prospective cohort study with patients with incident PH; 157 patients were diagnosed with RHC (34 chronic thromboembolic PH, 38 pulmonary arterial hypertension, 81 postcapillary PH, 4 miscellaneous PH), while 118 patients were labeled as probable postcapillary PH without hemodynamic confirmation. Multivariable analysis showed that older age (>60 years), left ventricular diastolic dysfunction grade 2-3, left atrial dilatation were independently associated with the decision to not perform an RHC, while presence of prior venous thromboembolic events or pulmonary arterial hypertension-associated conditions, right atrial dilatation, and tricuspid regurgitation velocity ≥3.7 m/s favor an RHC performance. Conclusions Older age and echocardiographic parameters of left heart disease were independently associated with the decision to not perform an RHC, while presence of prior venous thromboembolic events or pulmonary arterial hypertension-associated conditions, right atrial dilation, and severe PH on echocardiography favored an RHC performance. As such, especially elderly patients may be at an increased risk of diagnostic delays and missed diagnoses of treatable precapillary PH, which could lead to a worse prognosis.
MeSH term(s)	Aged ; Cardiac Catheterization/adverse effects ; Familial Primary Pulmonary Hypertension ; Hospitals, Community ; Humans ; Hypertension, Pulmonary/diagnosis ; Hypertension, Pulmonary/epidemiology ; Hypertension, Pulmonary/etiology ; Prospective Studies ; Pulmonary Arterial Hypertension
Language	English
Publishing date	2022-09-05
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2653953-6
ISSN	2047-9980 ; 2047-9980
ISSN (online)	2047-9980
ISSN	2047-9980
DOI	10.1161/JAHA.121.025143
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Article: Bacteria associated with toxic clonal cultures of the dinoflagellate Ostreopsis lenticularis.

Pérez-Guzmán, Lumarie / Pérez-Matos, Ana E / Rosado, William / Tosteson, Thomas R / Govind, Nadathur S

Marine biotechnology (New York, N.Y.)

2008 Volume 10, Issue 5, Page(s) 492–496

Abstract: The marine toxic dinoflagellate Ostreopsis lenticularis has been implicated as the major vector in ciguatera seafood poisoning on the southwest coast of Puerto Rico. Studies have demonstrated that associated bacteria play a role in the ciguatoxin ... ...

Abstract	The marine toxic dinoflagellate Ostreopsis lenticularis has been implicated as the major vector in ciguatera seafood poisoning on the southwest coast of Puerto Rico. Studies have demonstrated that associated bacteria play a role in the ciguatoxin production and that different clonal cultures of O. lenticularis harbor different culturable bacteria. In this study, more than 125 associated bacteria from two toxic clonal cultures of O. lenticularis (no. 302 and no. 303) were analyzed utilizing polymerase chain reaction amplification of the partial small subunit ribosomal DNA (rRNA), denaturing gradient gel electrophoresis, and DNA sequencing. Approximately 50% of total bacteria identified in both cultures were a single species belonging to the Cytophaga-Flavobacter-Bacteroides complex. This bacterium was also found in six new O. lenticularis clonal cultures established 10 years after the original cultures used in this study and absent from a clonal culture of a different dinoflagellate species. The data presented here indicate a persistent and apparently specific association of this bacterium with O. lenticularis, which makes it a candidate involved in ciguatoxin production.
MeSH term(s)	Animals ; Bacteria/classification ; Bacteria/genetics ; Bacteria/growth & development ; Bacteria/isolation & purification ; Clone Cells ; Dinoflagellida/microbiology ; Marine Toxins ; Phylogeny ; RNA, Ribosomal, 16S/genetics
Chemical Substances	Marine Toxins ; RNA, Ribosomal, 16S
Language	English
Publishing date	2008-03-26
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	1479877-3
ISSN	1436-2236 ; 1436-2228
ISSN (online)	1436-2236
ISSN	1436-2228
DOI	10.1007/s10126-008-9088-7
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Article: Impairment of cobalt-induced riboflavin biosynthesis in a Debaryomyces hansenii mutant.

Seda-Miró, Jasmine M / Arroyo-González, Nancy / Pérez-Matos, Ana / Govind, Nadathur S

Canadian journal of microbiology

2007 Volume 53, Issue 11, Page(s) 1272–1277

Abstract: Flavinogenic yeasts such as Debaryomyces hansenii overproduce riboflavin (RF) in the presence of heavy metals. Growth and RF production were compared between wild-type D. hansenii and a RF production-impaired metal-tolerant ura3 mutant in the presence of ...

Abstract	Flavinogenic yeasts such as Debaryomyces hansenii overproduce riboflavin (RF) in the presence of heavy metals. Growth and RF production were compared between wild-type D. hansenii and a RF production-impaired metal-tolerant ura3 mutant in the presence of sublethal cobalt(II) concentrations. Debaryomyces hansenii (wild type) exhibits an extended lag phase with an increase in RF synthesis. Supplementation of exogenous uracil shortened the lag phase at the highest concentration of cobalt(II) used, suggesting that uracil has a possible role in metal acclimation. The D. hansenii ura3 mutant isolated by chemical mutagenesis exhibited a higher level of metal tolerance, no extended lag phase, and no marked increase in RF synthesis. Transformation of the mutant with the URA3 gene isolated from Saccharyomyces cerevisiae or D. hansenii did not restore wild-type characteristics, suggesting a second mutation that impairs RF oversynthesis. Our results demonstrate that growth, metal sensitivity, and RF biosynthesis are linked.
MeSH term(s)	Cobalt/pharmacology ; Gene Expression Regulation, Fungal ; Mutation/genetics ; Riboflavin/biosynthesis ; Saccharomycetales/drug effects ; Saccharomycetales/genetics ; Saccharomycetales/growth & development ; Saccharomycetales/metabolism
Chemical Substances	Cobalt (3G0H8C9362) ; Riboflavin (TLM2976OFR)
Language	English
Publishing date	2007-11
Publishing country	Canada
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	280534-0
ISSN	1480-3275 ; 0008-4166
ISSN (online)	1480-3275
ISSN	0008-4166
DOI	10.1139/W07-098
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Article: Bacteria Associated with Toxic Clonal Cultures of the Dinoflagellate Ostreopsis lenticularis

Pérez-Guzmán, Lumarie / Pérez-Matos, Ana E / Rosado, William / Tosteson, Thomas R / Govind, Nadathur S

Marine biotechnology. 2008 Sept., v. 10, no. 5

2008

Abstract	The marine toxic dinoflagellate Ostreopsis lenticularis has been implicated as the major vector in ciguatera seafood poisoning on the southwest coast of Puerto Rico. Studies have demonstrated that associated bacteria play a role in the ciguatoxin production and that different clonal cultures of O. lenticularis harbor different culturable bacteria. In this study, more than 125 associated bacteria from two toxic clonal cultures of O. lenticularis (no. 302 and no. 303) were analyzed utilizing polymerase chain reaction amplification of the partial small subunit ribosomal DNA (rRNA), denaturing gradient gel electrophoresis, and DNA sequencing. Approximately 50% of total bacteria identified in both cultures were a single species belonging to the Cytophaga-Flavobacter-Bacteroides complex. This bacterium was also found in six new O. lenticularis clonal cultures established 10 years after the original cultures used in this study and absent from a clonal culture of a different dinoflagellate species. The data presented here indicate a persistent and apparently specific association of this bacterium with O. lenticularis, which makes it a candidate involved in ciguatoxin production.
Keywords	Dinophyceae ; bacteria ; ciguatoxin ; denaturing gradient gel electrophoresis ; polymerase chain reaction ; ribosomal DNA ; ribosomal RNA ; sequence analysis ; toxicity ; Puerto Rico
Language	English
Dates of publication	2008-09
Size	p. 492-496.
Publisher	Springer-Verlag
Publishing place	New York
Document type	Article
ZDB-ID	1479877-3
ISSN	1436-2236 ; 1436-2228
ISSN (online)	1436-2236
ISSN	1436-2228
DOI	10.1007/s10126-008-9088-7
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