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  1. Article ; Online: Generation of a transparent killifish line through multiplex CRISPR/Cas9mediated gene inactivation.

    Krug, Johannes / Perner, Birgit / Albertz, Carolin / Mörl, Hanna / Hopfenmüller, Vera L / Englert, Christoph

    eLife

    2023  Volume 12

    Abstract: Body pigmentation is a limitation ... ...

    Abstract Body pigmentation is a limitation for
    MeSH term(s) Animals ; Fundulidae ; Zebrafish/genetics ; Clustered Regularly Interspaced Short Palindromic Repeats ; Aging/genetics ; Longevity/physiology ; Mammals/genetics
    Language English
    Publishing date 2023-02-23
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2687154-3
    ISSN 2050-084X ; 2050-084X
    ISSN (online) 2050-084X
    ISSN 2050-084X
    DOI 10.7554/eLife.81549
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Wt1 Positive dB4 Neurons in the Hindbrain Are Crucial for Respiration.

    Schnerwitzki, Danny / Hayn, Christian / Perner, Birgit / Englert, Christoph

    Frontiers in neuroscience

    2020  Volume 14, Page(s) 529487

    Abstract: Central pattern generator (CPG) networks coordinate the generation of rhythmic activity such as locomotion and respiration. Their development is driven by various transcription factors, one of which is the Wilms tumor protein (Wt1). It is present in dI6 ... ...

    Abstract Central pattern generator (CPG) networks coordinate the generation of rhythmic activity such as locomotion and respiration. Their development is driven by various transcription factors, one of which is the Wilms tumor protein (Wt1). It is present in dI6 neurons of the mouse spinal cord, and involved in the coordination of locomotion. Here we report about the presence of Wt1 in neurons of the caudoventral medulla oblongata and their impact on respiration. By employing immunohistofluorescence staining, we were able to characterize these Wt1 positive (+) cells as dB4 neurons. The temporal occurrence of Wt1 suggests a role for this transcription factor in the differentiation of dB4 neurons during embryonic and postnatal development. Conditional knockout of
    Language English
    Publishing date 2020-11-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2411902-7
    ISSN 1662-453X ; 1662-4548
    ISSN (online) 1662-453X
    ISSN 1662-4548
    DOI 10.3389/fnins.2020.529487
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: The Wilms Tumor Gene

    Hopfenmüller, Vera L / Perner, Birgit / Reuter, Hanna / Bates, Thomas J D / Große, Andreas / Englert, Christoph

    Frontiers in cell and developmental biology

    2022  Volume 9, Page(s) 809962

    Abstract: The Wilms tumor suppressor ... ...

    Abstract The Wilms tumor suppressor gene
    Language English
    Publishing date 2022-01-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2021.809962
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Zebrafish Wtx is a negative regulator of Wnt signaling but is dispensable for embryonic development and organ homeostasis.

    Große, Andreas / Perner, Birgit / Naumann, Uta / Englert, Christoph

    Developmental dynamics : an official publication of the American Association of Anatomists

    2019  Volume 248, Issue 9, Page(s) 866–881

    Abstract: Background: The X-chromosomally linked gene WTX is a human disease gene and a member of the AMER family. Mutations in WTX are found in Wilms tumor, a form of pediatric kidney cancer and in patients suffering from OSCS (Osteopathia striata with cranial ... ...

    Abstract Background: The X-chromosomally linked gene WTX is a human disease gene and a member of the AMER family. Mutations in WTX are found in Wilms tumor, a form of pediatric kidney cancer and in patients suffering from OSCS (Osteopathia striata with cranial sclerosis), a sclerosing bone disorder. Functional data suggest WTX to be an inhibitor of the Wnt/β-catenin signaling pathway. Deletion of Wtx in mouse leads to perinatal death, impeding the analysis of its physiological role.
    Results: To gain insights into the function of Wtx in development and homeostasis we have used zebrafish as a model and performed both knockdown and knockout studies using morpholinos and transcription activator-like effector nucleases (TALENs), respectively. Wtx knockdown led to increased Wnt activity and embryonic dorsalization. Also, wtx mutants showed a transient upregulation of Wnt target genes in the context of caudal fin regeneration. Surprisingly, however, wtx as well as wtx/amer2/amer3 triple mutants developed normally, were fertile and did not show any anomalies in organ maintenance.
    Conclusions: Our data show that members of the zebrafish wtx/amer gene family, while sharing a partially overlapping expression pattern do not compensate for each other. This observation demonstrates a remarkable robustness during development and regeneration in zebrafish.
    MeSH term(s) Adaptor Proteins, Signal Transducing/genetics ; Adaptor Proteins, Signal Transducing/physiology ; Animals ; Embryo, Nonmammalian ; Embryonic Development ; Homeostasis ; Membrane Proteins/genetics ; Membrane Proteins/physiology ; Mutant Proteins/pharmacology ; Nuclear Proteins/genetics ; Nuclear Proteins/physiology ; Regeneration ; Wnt Signaling Pathway/drug effects ; Zebrafish ; Zebrafish Proteins/genetics ; Zebrafish Proteins/physiology
    Chemical Substances Adaptor Proteins, Signal Transducing ; Membrane Proteins ; Mutant Proteins ; Nuclear Proteins ; Zebrafish Proteins ; amer1 protein, zebrafish
    Language English
    Publishing date 2019-07-27
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1102541-4
    ISSN 1097-0177 ; 1058-8388
    ISSN (online) 1097-0177
    ISSN 1058-8388
    DOI 10.1002/dvdy.84
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ub I Zs.60: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 64: Show issues

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  5. Article ; Online: Immunofluorescence Staining of Wt1 on Sections of Zebrafish Embryos and Larvae.

    Perner, Birgit / Englert, Christoph

    Methods in molecular biology (Clifton, N.J.)

    2016  Volume 1467, Page(s) 129–132

    Abstract: Immunohistochemistry is one of the most powerful tools for direct visualization of distribution and localization of gene products. The presented protocol provides an opportunity to determine the localization patterns of Wt1 in zebrafish via antibody ... ...

    Abstract Immunohistochemistry is one of the most powerful tools for direct visualization of distribution and localization of gene products. The presented protocol provides an opportunity to determine the localization patterns of Wt1 in zebrafish via antibody staining.
    Language English
    Publishing date 2016
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-4023-3_11
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Aging Activates the Immune System and Alters the Regenerative Capacity in the Zebrafish Heart.

    Reuter, Hanna / Perner, Birgit / Wahl, Florian / Rohde, Luise / Koch, Philipp / Groth, Marco / Buder, Katrin / Englert, Christoph

    Cells

    2022  Volume 11, Issue 3

    Abstract: Age-associated organ failure and degenerative diseases have a major impact on human health. Cardiovascular dysfunction has an increasing prevalence with age and is one of the leading causes of death. In contrast to humans, zebrafish have extraordinary ... ...

    Abstract Age-associated organ failure and degenerative diseases have a major impact on human health. Cardiovascular dysfunction has an increasing prevalence with age and is one of the leading causes of death. In contrast to humans, zebrafish have extraordinary regeneration capacities of complex organs including the heart. In addition, zebrafish has recently become a model organism in research on aging. Here, we have compared the ventricular transcriptome as well as the regenerative capacity after cryoinjury of old and young zebrafish hearts. We identified the immune system as activated in old ventricles and found muscle organization to deteriorate upon aging. Our data show an accumulation of immune cells, mostly macrophages, in the old zebrafish ventricle. Those immune cells not only increased in numbers but also showed morphological and behavioral changes with age. Our data further suggest that the regenerative response to cardiac injury is generally impaired and much more variable in old fish. Collagen in the wound area was already significantly enriched in old fish at 7 days post injury. Taken together, these data indicate an 'inflammaging'-like process in the zebrafish heart and suggest a change in regenerative response in the old.
    MeSH term(s) Aging ; Animals ; Cell Proliferation ; Heart/physiology ; Macrophages ; Zebrafish/physiology
    Language English
    Publishing date 2022-01-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells11030345
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Book ; Online: Simulation of heart infarction by laser microbeams and induction of arrhythmias by optical tweezers

    Perner, Birgit

    2003  

    Institution Institut für Molekulare Biotechnologie / Dept. Single Cell and Single Molecule Techniques
    Author's details Dept. Single Cell and Single Molecule Techniques, Institute of Molecular Biotechnology. Birgit Perner
    Language English
    Size Online-Ressource (10 S., 0,98 MB), Ill., graph. Darst.
    Publisher Technische Informationsbibliothek u. Universitätsbibliothek
    Publishing place Hannover ; Jena
    Document type Book ; Online
    Note Förderkennzeichen BMBF 13N7506 ; IMD-Felder maschinell generiert ; Unterschiede zwischen dem gedruckten Dokument und der elektronische Ressource können nicht ausgeschlossen werden
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  8. Article: Analysis of zebrafish kidney development with time-lapse imaging using a dissecting microscope equipped for optical sectioning

    Perner, Birgit / Schnerwitzki, Danny / Graf, Michael / Englert, Christoph

    Journal of visualized experiments. 2016 Apr. 07, , no. 110

    2016  

    Abstract: In order to understand organogenesis, the spatial and temporal alterations that occur during development of tissues need to be recorded. The method described here allows time-lapse analysis of normal and impaired kidney development in zebrafish embryos ... ...

    Abstract In order to understand organogenesis, the spatial and temporal alterations that occur during development of tissues need to be recorded. The method described here allows time-lapse analysis of normal and impaired kidney development in zebrafish embryos by using a fluorescence dissecting microscope equipped for structured illumination and z-stack acquisition. To visualize nephrogenesis, transgenic zebrafish (Tg(wt1b:GFP)) with fluorescently labeled kidney structures were used. Renal defects were triggered by injection of an antisense morpholino oligonucleotide against the Wilms tumor gene wt1a, a factor known to be crucial for kidney development. The advantage of the experimental setup is the combination of a zoom microscope with simple strategies for re-adjusting movements in x, y or z direction without additional equipment. To circumvent focal drift that is induced by temperature variations and mechanical vibrations, an autofocus strategy was applied instead of utilizing a usually required environmental chamber. In order to re-adjust the positional changes due to a xy-drift, imaging chambers with imprinted relocation grids were employed. In comparison to more complex setups for time-lapse recording with optical sectioning such as confocal laser scanning or light sheet microscopes, a zoom microscope is easy to handle. Besides, it offers dissecting microscope-specific benefits such as high depth of field and an extended working distance. The method to study organogenesis presented here can also be used with fluorescence stereo microscopes not capable of optical sectioning. Although limited for high-throughput, this technique offers an alternative to more complex equipment that is normally used for time-lapse recording of developing tissues and organ dynamics.
    Keywords Danio rerio ; embryo (animal) ; fluorescence ; genes ; genetically modified organisms ; image analysis ; kidneys ; lighting ; microscopes ; neoplasms ; oligonucleotides ; organogenesis ; temperature ; tissues
    Language English
    Dates of publication 2016-0407
    Size p. e53921.
    Publishing place Journal of Visualized Experiments
    Document type Article
    ZDB-ID 2259946-0
    ISSN 1940-087X
    ISSN 1940-087X
    DOI 10.3791/53921
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning.

    Perner, Birgit / Schnerwitzki, Danny / Graf, Michael / Englert, Christoph

    Journal of visualized experiments : JoVE

    2016  , Issue 110, Page(s) e53921

    Abstract: In order to understand organogenesis, the spatial and temporal alterations that occur during development of tissues need to be recorded. The method described here allows time-lapse analysis of normal and impaired kidney development in zebrafish embryos ... ...

    Abstract In order to understand organogenesis, the spatial and temporal alterations that occur during development of tissues need to be recorded. The method described here allows time-lapse analysis of normal and impaired kidney development in zebrafish embryos by using a fluorescence dissecting microscope equipped for structured illumination and z-stack acquisition. To visualize nephrogenesis, transgenic zebrafish (Tg(wt1b:GFP)) with fluorescently labeled kidney structures were used. Renal defects were triggered by injection of an antisense morpholino oligonucleotide against the Wilms tumor gene wt1a, a factor known to be crucial for kidney development. The advantage of the experimental setup is the combination of a zoom microscope with simple strategies for re-adjusting movements in x, y or z direction without additional equipment. To circumvent focal drift that is induced by temperature variations and mechanical vibrations, an autofocus strategy was applied instead of utilizing a usually required environmental chamber. In order to re-adjust the positional changes due to a xy-drift, imaging chambers with imprinted relocation grids were employed. In comparison to more complex setups for time-lapse recording with optical sectioning such as confocal laser scanning or light sheet microscopes, a zoom microscope is easy to handle. Besides, it offers dissecting microscope-specific benefits such as high depth of field and an extended working distance. The method to study organogenesis presented here can also be used with fluorescence stereo microscopes not capable of optical sectioning. Although limited for high-throughput, this technique offers an alternative to more complex equipment that is normally used for time-lapse recording of developing tissues and organ dynamics.
    MeSH term(s) Animals ; Animals, Genetically Modified ; Developmental Biology ; Embryo, Nonmammalian/embryology ; Histocytological Preparation Techniques ; Kidney/embryology ; Lasers ; Microscopy, Confocal/methods ; Morpholinos/genetics ; Oligonucleotides, Antisense ; Organogenesis ; Time-Lapse Imaging ; WT1 Proteins/genetics ; Zebrafish/embryology ; Zebrafish Proteins/genetics
    Chemical Substances Morpholinos ; Oligonucleotides, Antisense ; WT1 Proteins ; Wt1a protein, zebrafish ; Zebrafish Proteins
    Language English
    Publishing date 2016-04-07
    Publishing country United States
    Document type Journal Article ; Video-Audio Media
    ISSN 1940-087X
    ISSN (online) 1940-087X
    DOI 10.3791/53921
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Function and Regulation of the Wilms' Tumor Suppressor 1 (WT1) Gene in Fish.

    Perner, Birgit / Bates, Thomas J D / Naumann, Uta / Englert, Christoph

    Methods in molecular biology (Clifton, N.J.)

    2016  Volume 1467, Page(s) 119–128

    Abstract: The Wilms' tumor suppressor gene Wt1 is highly conserved among vertebrates. In contrast to mammals, most fish species possess two wt1 paralogs that have been named wt1a and wt1b. Concerning wt1 in fish, most work so far has been done using zebrafish, ... ...

    Abstract The Wilms' tumor suppressor gene Wt1 is highly conserved among vertebrates. In contrast to mammals, most fish species possess two wt1 paralogs that have been named wt1a and wt1b. Concerning wt1 in fish, most work so far has been done using zebrafish, focusing on the embryonic kidney, the pronephros. In this chapter we will describe the structure and development of the pronephros as well as the role that the wt1 genes play in the embryonic zebrafish kidney. We also discuss Wt1 target genes and describe the potential function of the Wt1 proteins in the adult kidney. Finally we will summarize data on the role of Wt1 outside of the kidney.
    Language English
    Publishing date 2016
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-4023-3_10
    Database MEDical Literature Analysis and Retrieval System OnLINE

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