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Article ; Online: To Explore the Stem Cells Homing to GBM

Sergey Tsibulnikov / Natalya M. Drefs / Peter S. Timashev / Ilya V. Ulasov

Biomedicines, Vol 10, Iss 986, p

The Rise to the Occasion

2022 Volume 986

Abstract: Multiple efforts are currently underway to develop targeted therapeutic deliveries to the site of glioblastoma progression. The use of carriers represents advancement in the delivery of various therapeutic agents as a new approach in neuro-oncology. ... ...

Abstract	Multiple efforts are currently underway to develop targeted therapeutic deliveries to the site of glioblastoma progression. The use of carriers represents advancement in the delivery of various therapeutic agents as a new approach in neuro-oncology. Mesenchymal stem cells (MSCs) and neural stem cells (NSCs) are used because of their capability in migrating and delivering therapeutic payloads to tumors. Two of the main properties that carrier cells should possess are their ability to specifically migrate from the bloodstream and low immunogenicity. In this article, we also compared the morphological and molecular features of each type of stem cell that underlie their migration capacity to glioblastoma. Thus, the major focus of the current review is on proteins and lipid molecules that are released by GBM to attract stem cells.
Keywords	stem cells ; glioblastoma ; CXCR4 ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2022-04-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: 3D nanomechanical mapping of subcellular and sub-nuclear structures of living cells by multi-harmonic AFM with long-tip microcantilevers

Yuri M. Efremov / Daniel M. Suter / Peter S. Timashev / Arvind Raman

Scientific Reports, Vol 12, Iss 1, Pp 1-

2022 Volume 11

Abstract: Abstract Recent developments such as multi-harmonic Atomic Force Microscopy (AFM) techniques have enabled fast, quantitative mapping of nanomechanical properties of living cells. Due to their high spatiotemporal resolution, these methods provide new ... ...

Abstract	Abstract Recent developments such as multi-harmonic Atomic Force Microscopy (AFM) techniques have enabled fast, quantitative mapping of nanomechanical properties of living cells. Due to their high spatiotemporal resolution, these methods provide new insights into changes of mechanical properties of subcellular structures due to disease or drug response. Here, we propose three new improvements to significantly improve the resolution, identification, and mechanical property quantification of sub-cellular and sub-nuclear structures using multi-harmonic AFM on living cells. First, microcantilever tips are streamlined using long-carbon tips to minimize long-range hydrodynamic interactions with the cell surface, to enhance the spatial resolution of nanomechanical maps and minimize hydrodynamic artifacts. Second, simultaneous Spinning Disk Confocal Microscopy (SDC) with live-cell fluorescent markers enables the unambiguous correlation between observed heterogeneities in nanomechanical maps with subcellular structures. Third, computational approaches are then used to estimate the mechanical properties of sub-nuclear structures. Results are demonstrated on living NIH 3T3 fibroblasts and breast cancer MDA-MB-231 cells, where properties of nucleoli, a deep intracellular structure, were assessed. The integrated approach opens the door to study the mechanobiology of sub-cellular structures during disease or drug response.
Keywords	Medicine ; R ; Science ; Q
Subject code	610
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Aliphatic Polybenzimidazoles

Bato Ch. Kholkhoev / Zakhar A. Matveev / Kseniia N. Bardakova / Peter S. Timashev / Vitaliy F. Burdukovskii

Polymers, Vol 15, Iss 1399, p

Synthesis, Characterization and High-Temperature Shape-Memory Performance

2023 Volume 1399

Abstract: A series of aliphatic polybenzimidazoles (PBIs) with methylene groups of varying length were synthesized by the high-temperature polycondensation of 3,3′-diaminobenzidine (DAB) and the corresponding aliphatic dicarboxylic acid in Eaton’s reagent. The ... ...

Abstract	A series of aliphatic polybenzimidazoles (PBIs) with methylene groups of varying length were synthesized by the high-temperature polycondensation of 3,3′-diaminobenzidine (DAB) and the corresponding aliphatic dicarboxylic acid in Eaton’s reagent. The influence of the length of the methylene chain on PBIs’ properties was investigated by solution viscometry, thermogravimetric analysis, mechanical testing and dynamic mechanical analysis. All PBIs exhibited high mechanical strength (up to 129.3 ± 7.1 MPa), glass transition temperature (≥200 °C) and thermal decomposition temperature (≥460 °C). Moreover, all of the synthesized aliphatic PBIs possess a shape-memory effect, which is a result of the presence of soft aliphatic segments and rigid bis -benzimidazole groups in the macromolecules, as well as strong intermolecular hydrogen bonds that serve as non-covalent crosslinks. Among the studied polymers, the PBI based on DAB and dodecanedioic acid has high adequate mechanical and thermal properties and demonstrates the highest shape-fixity ratio and shape-recovery ratio of 99.6% and 95.6%, respectively. Because of these properties, aliphatic PBIs have great potential to be used as high-temperature materials for application in different high-tech fields, including the aerospace industry and structural component industries.
Keywords	polybenzimidazoles ; high-temperature shape-memory polymers ; mechanical properties ; thermally stable polymers ; physical crosslinking ; hydrogen bonding ; Organic chemistry ; QD241-441
Subject code	660
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Towards clinical translation of the cell sheet engineering

Irina M. Zurina / Victoria S. Presniakova / Denis V. Butnaru / Peter S. Timashev / Yury A. Rochev / Xing-Jie Liang

Smart Materials in Medicine, Vol 4, Iss , Pp 146-

Technological aspects

2023 Volume 159

Abstract: Cell sheet engineering is a rapidly growing field of tissue engineering and regenerative medicine. The ease of cell sheet obtainment techniques and the resulting unique characteristics and microenvironment of these multicellular structures give rise to ... ...

Abstract	Cell sheet engineering is a rapidly growing field of tissue engineering and regenerative medicine. The ease of cell sheet obtainment techniques and the resulting unique characteristics and microenvironment of these multicellular structures give rise to the wide range of their in vivo application. At the same time, there are also macroscale cell sheet properties such as thickness, shrinkage after detachment due to cytoskeleton relaxation, and resulting mechanical characteristics. The main topic of this review is the discussion of these properties and how they define the need to use special approaches to manipulating cell sheets during stacking several structures, transferring them to surgical sites, or cryopreserving them. We aimed to systematize the existing techniques of cell sheet transferring, and describe their principles, advantages, and drawbacks regarding cell sheet application during surgical procedures on various tissues and organs. Attention is also paid to such aspects and details as cell sheet positioning in vivo, their ability to spontaneous adhesion, and the requirement for additional fixation at particular surgical sites. Finally, the last section of this review covers the subject of cell sheet cryopreservation – the discussion of freezing and thawing protocols, the variety of cryoprotectants and their mixtures, as well as special requirements such as cryoprotectant loading systems, and cell sheet supporting systems that also stem from their unique macroscale characteristics. Altogether, this systematized review of existing technological approaches related to cell sheet application in vivo can be potentially helpful for the new and expert researchers in this area of tissue engineering.
Keywords	Cell sheets ; Regenerative medicine ; Macroscale characteristics ; Transferring techniques ; Cryopreservation ; Technology ; T
Subject code	670
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	KeAi Communications Co., Ltd.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Metabolome-Driven Regulation of Adenovirus-Induced Cell Death

Anastasia Laevskaya / Anton Borovjagin / Peter S. Timashev / Maciej S. Lesniak / Ilya Ulasov

International Journal of Molecular Sciences, Vol 22, Iss 464, p

2021 Volume 464

Abstract: A viral infection that involves virus invasion, protein synthesis, and virion assembly is typically accompanied by sharp fluctuations in the intracellular levels of metabolites. Under certain conditions, dramatic metabolic shifts can result in various ... ...

Abstract	A viral infection that involves virus invasion, protein synthesis, and virion assembly is typically accompanied by sharp fluctuations in the intracellular levels of metabolites. Under certain conditions, dramatic metabolic shifts can result in various types of cell death. Here, we review different types of adenovirus-induced cell death associated with changes in metabolic profiles of the infected cells. As evidenced by experimental data, in most cases changes in the metabolome precede cell death rather than represent its consequence. In our previous study, the induction of autophagic cell death was observed following adenovirus-mediated lactate production, acetyl-CoA accumulation, and ATP release, while apoptosis was demonstrated to be modulated by alterations in acetate and asparagine metabolism. On the other hand, adenovirus-induced ROS production and ATP depletion were demonstrated to play a significant role in the process of necrotic cell death. Interestingly, the accumulation of ceramide compounds was found to contribute to the induction of all the three types of cell death mentioned above. Eventually, the characterization of metabolite analysis could help in uncovering the molecular mechanism of adenovirus-mediated cell death induction and contribute to the development of efficacious oncolytic adenoviral vectors.
Keywords	autophagy ; viruses ; metabolites ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Structure and Dynamics of Inhomogeneities in Aqueous Solutions of Graft Copolymers of N-Isopropylacrylamide with Lactide (P(NIPAM- graft -PLA)) by Spin Probe EPR Spectroscopy

Ekaterina M. Zubanova / Tatiana A. Ivanova / Evgenii A. Ksendzov / Sergei V. Kostjuk / Peter S. Timashev / Mikhail Ya. Melnikov / Elena N. Golubeva

Polymers, Vol 14, Iss 4746, p

2022 Volume 4746

Abstract: Coil-to-globule transition and dynamics of inhomogeneities in aqueous solutions of graft copolymers of NIPAM with different content of oligolactide groups were studied using spin probe continuous wave EPR spectroscopy. The technique of the suppressing of ...

Abstract	Coil-to-globule transition and dynamics of inhomogeneities in aqueous solutions of graft copolymers of NIPAM with different content of oligolactide groups were studied using spin probe continuous wave EPR spectroscopy. The technique of the suppressing of TEMPO as spin probe by spin exchange with Cu 2+ ions was applied. This approach allowed us to detect individual EPR spectra of the probe in collapsed globules and estimate its magnetic and dynamic parameters reliably. The formation of inhomogeneities at temperatures lower than the volume phase transition temperature measured via transmission, and differential scanning calorimetry was fixed. An increase in oligolactide content in copolymers leads to the formation of looser globules, allowing for the exchange of the probe molecules between the globules and the external solution.
Keywords	thermoresponsive polymers ; electron paramagnetic resonance ; nitroxides ; inhomogeneities ; coil-to-globule ; spin probe ; Organic chemistry ; QD241-441
Language	English
Publishing date	2022-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Implantation of Various Cell-Free Matrixes Does Not Contribute to the Restoration of Hyaline Cartilage within Full-Thickness Focal Defects

Shabnam I. Ibragimova / Ekaterina V. Medvedeva / Irina A. Romanova / Leonid P. Istranov / Elena V. Istranova / Aleksey V. Lychagin / Andrey A. Nedorubov / Peter S. Timashev / Vladimir I. Telpukhov / Andrei S. Chagin

International Journal of Molecular Sciences, Vol 23, Iss 292, p

2022 Volume 292

Abstract: Articular cartilage is a highly organized tissue that has a limited ability to heal. Tissue engineering is actively exploited for joint tissue reconstruction in numerous cases of articular cartilage degeneration associated with trauma, arthrosis, ... ...

Abstract	Articular cartilage is a highly organized tissue that has a limited ability to heal. Tissue engineering is actively exploited for joint tissue reconstruction in numerous cases of articular cartilage degeneration associated with trauma, arthrosis, rheumatoid arthritis, and osteoarthritis. However, the optimal scaffolds for cartilage repair are not yet identified. Here we have directly compared five various scaffolds, namely collagen-I membrane, collagen-II membrane, decellularized cartilage, a cellulose-based implant, and commercially available Chondro-Gide ® (Geistlich Pharma AG, Wolhusen, Switzerland) collagen membrane. The scaffolds were implanted in osteochondral full-thickness defects, formed on adult Wistar rats using a hand-held cutter with a diameter of 2.0 mm and a depth of up to the subchondral bone. The congruence of the articular surface was almost fully restored by decellularized cartilage and collagen type II-based scaffold. The most vivid restoration was observed 4 months after the implantation. The formation of hyaline cartilage was not detected in any of the groups. Despite cellular infiltration into scaffolds being observed in each group except cellulose, neither chondrocytes nor chondro-progenitors were detected. We concluded that for restoration of hyaline cartilage, scaffolds have to be combined either with cellular therapy or morphogens promoting chondrogenic differentiation.
Keywords	articular cartilage ; full-thickness defect ; scaffold ; collagen membrane ; decellularized cartilage ; cellulose ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	616
Language	English
Publishing date	2022-12-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Electrospinning vs. Electro-Assisted Solution Blow Spinning for Fabrication of Fibrous Scaffolds for Tissue Engineering

Tatiana S. Demina / Evgeniy N. Bolbasov / Maria A. Peshkova / Yuri M. Efremov / Polina Y. Bikmulina / Aisylu V. Birdibekova / Tatiana N. Popyrina / Nastasia V. Kosheleva / Sergei I. Tverdokhlebov / Peter S. Timashev / Tatiana A. Akopova

Polymers, Vol 14, Iss 5254, p

2022 Volume 5254

Abstract: Biodegradable polymeric fibrous non-woven materials are widely used type of scaffolds for tissue engineering. Their morphology and properties could be controlled by composition and fabrication technology. This work is aimed at development of fibrous ... ...

Abstract	Biodegradable polymeric fibrous non-woven materials are widely used type of scaffolds for tissue engineering. Their morphology and properties could be controlled by composition and fabrication technology. This work is aimed at development of fibrous scaffolds from a multicomponent polymeric system containing biodegradable synthetic (polylactide, polycaprolactone) and natural (gelatin, chitosan) components using different methods of non-woven mats fabrication: electrospinning and electro-assisted solution blow spinning. The effect of the fabrication technique of the fibrous materials onto their morphology and properties, including the ability to support adhesion and growth of cells, was evaluated. The mats fabricated using electrospinning technology consist of randomly oriented monofilament fibers, while application of solution blow spinning gave a rise to chaotically arranged multifilament fibers. Cytocompatibility of all fabricated fibrous mats was confirmed using in vitro analysis of metabolic activity, proliferative capacity and morphology of NIH 3T3 cell line. Live/Dead assay revealed the formation of the highest number of cell–cell contacts in the case of multifilament sample formed by electro-assisted solution blow spinning technology.
Keywords	polylactide ; non-woven mats ; electrospinning ; tissue engineering ; solution blow spinning ; cell growth ; Organic chemistry ; QD241-441
Language	English
Publishing date	2022-12-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: The Duo of Osteogenic and Angiogenic Differentiation in ADSC-Derived Spheroids

Anastasiya A. Gorkun / Daria P. Revokatova / Irina M. Zurina / Denis A. Nikishin / Polina Y. Bikmulina / Peter S. Timashev / Anastasiya I. Shpichka / Nastasia V. Kosheleva / Tamara D. Kolokoltsova / Irina N. Saburina

Frontiers in Cell and Developmental Biology, Vol

2021 Volume 9

Abstract: Bone formation during embryogenesis is driven by interacting osteogenesis and angiogenesis with parallel endothelial differentiation. Thence, all in vitro bioengineering techniques are aimed at pre-vascularization of osteogenic bioequivalents to provide ... ...

Abstract	Bone formation during embryogenesis is driven by interacting osteogenesis and angiogenesis with parallel endothelial differentiation. Thence, all in vitro bioengineering techniques are aimed at pre-vascularization of osteogenic bioequivalents to provide better regeneration outcomes upon transplantation. Due to appearance of cell–cell and cell–matrix interactions, 3D cultures of adipose-derived stromal cells (ADSCs) provide a favorable spatial context for the induction of different morphogenesis processes, including vasculo-, angio-, and osteogenesis and, therefore, allow modeling their communication in vitro. However, simultaneous induction of multidirectional cell differentiation in spheroids from multipotent mesenchymal stromal cells (MMSCs) was not considered earlier. Here we show that arranging ADSCs into spheroids allows rapid and spontaneous acquiring of markers of both osteo- and angiogenesis compared with 2D culture. We further showed that this multidirectional differentiation persists in time, but is not influenced by classical protocols for osteo- or angio-differentiation. At the same time, ADSC-spheroids retain similar morphology and microarchitecture in different culture conditions. These findings can contribute to a better understanding of the fundamental aspects of autonomous regulation of differentiation processes and their cross-talks in artificially created self-organizing multicellular structures. This, in turn, can find a wide range of applications in the field of tissue engineering and regeneration.
Keywords	osteogenesis ; agiogenesis ; spheroid ; 3D culture ; tissue engineering ; adipose-derived stromal cells (ADSCs) ; Biology (General) ; QH301-705.5
Subject code	571
Language	English
Publishing date	2021-04-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effect of Chitosan on the Activity of Water-Soluble and Hydrophobic Porphyrin Photosensitizers Solubilized by Amphiphilic Polymers

Valeriya V. Kardumyan / Nadejda A. Aksenova / Victoria A. Timofeeva / Alexey V. Krivandin / Olga V. Shatalova / Alexander S. Dubovik / Irina G. Plashchina / Peter S. Timashev / Anna B. Solovieva

Polymers, Vol 13, Iss 1007, p

2021 Volume 1007

Abstract: In this work, we studied the photocatalytic activity of photosensitizers (PSs) of various natures solubilized with polyvinylpyrrolidone (PVP) and ternary block copolymer ethylene and propylene oxide Pluronic F127 (F127) in a model reaction of tryptophan ... ...

Abstract	In this work, we studied the photocatalytic activity of photosensitizers (PSs) of various natures solubilized with polyvinylpyrrolidone (PVP) and ternary block copolymer ethylene and propylene oxide Pluronic F127 (F127) in a model reaction of tryptophan photo-oxidation in water in the presence of chitosan (CT). Water-soluble compounds (dimegin and trisodium salt of chlorin e6 (Ce6)) and hydrophobic porphyrins (tetraphenylporphyrin (TPP) and its fluorine derivative (TPPF20)) were used as PSs. It was shown that the use of chitosan (Mw ~100 kDa) makes it possible to obtain a system whose activity is comparable to that of the photosensitizer-amphiphilic polymer systems. Thus, the previously observed drop in the photosensitizing activity of PS in the presence of a polysaccharide and amphiphilic polymers (AP) was absent in this case. At the same time, chitosan had practically no inhibitory effect on hydrophobic porphyrins solubilized by Pluronic F127.
Keywords	chitosan ; porphyrin ; Pluronic F127 ; polyvinylpyrrolidone ; tryptophan photo-oxidation ; Organic chemistry ; QD241-441
Subject code	540
Language	English
Publishing date	2021-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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