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Article: Spontaneous nerve activity and sensitivity in catfish ampullary electroreceptor organs after tetanus toxin application.

Struik, M L / Bretschneider, F / Peters, R C

Pflugers Archiv : European journal of physiology

2002 Volume 443, Issue 5-6, Page(s) 903–907

Abstract: The functioning of electroreceptor organs of Ictalurus sp. was investigated by inhibiting synaptic transmission by the administration of tetanus toxin in vitro. A piece of Ictalurus skin of about 20 mm diameter was mounted in an Ussing-type chamber. ... ...

Abstract	The functioning of electroreceptor organs of Ictalurus sp. was investigated by inhibiting synaptic transmission by the administration of tetanus toxin in vitro. A piece of Ictalurus skin of about 20 mm diameter was mounted in an Ussing-type chamber. After establishing the normal functioning of the organ, tetanus toxin (TeTx) was applied basolaterally for 150 min in 66.7 pM and 400 pM concentrations, while the single unit nerve activity was recorded extracellularly. Spontaneous spike activity and the sensitivity of the electroreceptor organs were measured. The results show that TeTx reduces sensitivity to less then 20% of its original value, whereas the spontaneous activity is unaffected by the treatment. This indicates that the afferent nerve is capable of generating impulses independent of receptor cell neurotransmitter release. In the discussion we suggest two alternative mechanisms for the emergence of the spontaneous spike activity.
MeSH term(s)	Action Potentials/drug effects ; Action Potentials/physiology ; Animals ; Catfishes ; Electric Organ/drug effects ; Electric Organ/physiology ; Electrophysiology ; In Vitro Techniques ; Reaction Time/drug effects ; Reaction Time/physiology ; Synaptic Transmission/drug effects ; Synaptic Transmission/physiology ; Tetanus Toxin/pharmacology
Chemical Substances	Tetanus Toxin
Language	English
Publishing date	2002-03
Publishing country	Germany
Document type	Journal Article
ZDB-ID	6380-0
ISSN	1432-2013 ; 0031-6768
ISSN (online)	1432-2013
ISSN	0031-6768
DOI	10.1007/s00424-001-0778-5
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Article: The electroreceptor organ of the catfish, Ictalurus melas, as a model for cisplatin-induced ototoxicity.

Peters, R C / Mommersteeg, P M / Heijmen, P S

Neuroscience

1999 Volume 91, Issue 2, Page(s) 745–751

Abstract: The ototoxic side-effects of the anti-cancer drug cisplatin (cis-diaminedichloroplatinum) have been widely investigated. However, the exact site of action remains unclear. In this study, the electroreceptor organ of the freshwater catfish Ictalurus melas ...

Abstract	The ototoxic side-effects of the anti-cancer drug cisplatin (cis-diaminedichloroplatinum) have been widely investigated. However, the exact site of action remains unclear. In this study, the electroreceptor organ of the freshwater catfish Ictalurus melas is used as a model for examining the acute effects of cisplatin. The sensory cells in the electroreceptor organ are homologous to the inner hair cells in the cochlea of mammals. The effects of cisplatin administration can be investigated by in vivo recording of the spike trains from the electroreceptor organ primary afferents. Exposure of electroreceptor organs to 330 microM cisplatin for 1 h causes the spontaneous activity to drop, the overall sensitivity to diminish and the shape of the frequency characteristics to change. These effects persist in the week after administration. Control levels have returned at day 22. These results demonstrate an acute and, with considerable hysteresis, reversible cisplatin effect on the electroreceptor organs, which is to a large extent consistent with the cisplatin-induced effects in isolated hair cells in mammals. The time-course of the effect supports the hypotheses that ion channels are blocked immediately by cisplatin administration, and that cisplatin metabolites disturb enzymatic cellular processes.
MeSH term(s)	Afferent Pathways/drug effects ; Afferent Pathways/physiology ; Analysis of Variance ; Animals ; Antineoplastic Agents/toxicity ; Cisplatin/toxicity ; Electric Organ/drug effects ; Electric Organ/physiology ; Female ; Hair Cells, Auditory, Inner/drug effects ; Ictaluridae ; Male ; Mammals ; Skin/innervation ; Time Factors
Chemical Substances	Antineoplastic Agents ; Cisplatin (Q20Q21Q62J)
Language	English
Publishing date	1999
Publishing country	United States
Document type	Journal Article
ZDB-ID	196739-3
ISSN	1873-7544 ; 0306-4522
ISSN (online)	1873-7544
ISSN	0306-4522
DOI	10.1016/s0306-4522(98)00658-7
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Article ; Online: Effects of dietary fats on red blood cell membrane insulin receptor in normo- and hypercholesterolemic miniature swine.

Bhathena, S J. / Berlin, E / McClure, D / Peters, R C.

The Journal of nutritional biochemistry

2002 Volume 12, Issue 9, Page(s) 529–535

Abstract: It has been demonstrated that the type of dietary fat affects insulin receptors in various tissues in normal humans and animals by altering membrane fluidity. This study compares the effects of n-3 fatty acids from fish oil and n-6 fatty acids from corn ... ...

Abstract	It has been demonstrated that the type of dietary fat affects insulin receptors in various tissues in normal humans and animals by altering membrane fluidity. This study compares the effects of n-3 fatty acids from fish oil and n-6 fatty acids from corn oil on red blood cell membrane insulin receptors in normal and hypercholesterolemic minipigs. A group of minipigs were made hypercholesterolemic by feeding cholesterol and lard for 2 months; the other group served as controls and was fed stock diet. Both groups were then fed experimental diets containing either corn oil or menhaden oil or a mixture of the two for 23 additional weeks. Blood was collected at 0, 2, 12 and 23 weeks after the start of the experimental diets and membranes were prepared from the red blood cells. Insulin binding to red blood cell membranes was measured by radioreceptor assay. Plasma insulin was measured by radioimmunoassay. Insulin binding to red blood cell membrane was compared with the fluidity of the membrane measured and reported earlier. There was no significant effect of cholesterol feeding on plasma insulin concentrations. After 23 weeks on experimental diet plasma insulin was significantly higher in minipigs fed menhaden oil compared to those fed corn oil. No such effect was observed in hypercholesterolemic minipigs. No significant effect of either hypercholesterolemia or fish oil was observed on red blood cell insulin binding. A significant negative relationship was observed between insulin binding and anisotropy at 4°C for all probes but at 37°C significant negative relationship was observed only with polar probes. The data suggest that n-3 fatty acids from fish oil significantly increases plasma insulin in minipigs compared to n-6 fatty acids from corn oil. However, the unsaturation has no significant effect on insulin receptors on erythrocytes. Similarly, prior hypercholesterolemic state also has no effect on plasma insulin levels or the insulin binding to red blood cell membranes.
Language	English
Publishing date	2002-01-17
Publishing country	United States
Document type	Journal Article
ZDB-ID	1014929-6
ISSN	1873-4847 ; 0955-2863
ISSN (online)	1873-4847
ISSN	0955-2863
DOI	10.1016/s0955-2863(01)00171-1
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Article: Electrophysiological demonstration of N-methyl-D-aspartate receptors at the afferent synapse of catfish electroreceptor organs.

Andrianov, G N / Bretschneider, F / Peters, R C

Neuroscience

1997 Volume 79, Issue 4, Page(s) 1231–1237

Abstract: An excitatory amino acid, most probably L-glutamate, acts as a neurotransmitter at the receptor cell--afferent fibre synapses in the ampullary electroreceptor organs of the freshwater catfish Ictalurus nebulosus. In the present study, we have used an ... ...

Abstract	An excitatory amino acid, most probably L-glutamate, acts as a neurotransmitter at the receptor cell--afferent fibre synapses in the ampullary electroreceptor organs of the freshwater catfish Ictalurus nebulosus. In the present study, we have used an electrophysiological approach to investigate the presence of N-methyl-D-aspartate receptors at this level. N-Methyl-D-aspartate, dissolved in an Mg(2+)-containing (normal) solution, had no effect on afferent activity, not even at 5 mM. However, addition of 5 mM N-methyl-D-aspartate to an Mg(2+)-free solution evoked an enduring increase in firing rate. The application of N-methyl-D-aspartate combined with electrical sine wave stimulation produced a firing increase in the primary afferents, even in the presence of Mg2+ (1.5 mM). Glycine (0.01-0.001 mM) significantly potentiated the N-methyl-D-aspartate responses. Addition of antagonists of the actions of N-methyl-D-aspartate, 7-chlorokynurenic acid, DL-2-amino-5-phosphonovaleric acid and ketamine in concentrations of 0.5-2.0 mM led to a decrease in resting and stimulus-evoked activity. 7-Chlorokynurenic acid also blocked the responses to application of N-methyl-D-aspartate. The glycine agonist D-serine (0.01 mM) prevented the 7-chlorokynurenic inhibitory effect. These results suggest the involvement of N-methyl-D-aspartate receptors in mediating the actions of L-glutamate at the afferent synapses of the electroreceptor organs of the catfish.
MeSH term(s)	Afferent Pathways/physiology ; Animals ; Electric Organ/physiology ; Electric Stimulation ; Ictaluridae ; Membrane Potentials/drug effects ; N-Methylaspartate/pharmacology ; Receptors, N-Methyl-D-Aspartate/drug effects ; Receptors, N-Methyl-D-Aspartate/physiology ; Synapses/physiology
Chemical Substances	Receptors, N-Methyl-D-Aspartate ; N-Methylaspartate (6384-92-5)
Language	English
Publishing date	1997-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	196739-3
ISSN	1873-7544 ; 0306-4522
ISSN (online)	1873-7544
ISSN	0306-4522
DOI	10.1016/s0306-4522(97)00036-5
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Article: Vincristine disturbs spontaneous firing of the afferent nerve fibre in ampullary electroreceptor organs.

Heijmen, P S / Kalmeijer, D / Peters, R C

Neuroscience

1996 Volume 72, Issue 2, Page(s) 585–591

Abstract: Ampullary electroreceptor organs of the catfish were apically exposed to 0.3 mM vincristine in order to investigate the part played by the microtubular system in stimulus transduction. The main effects were repetitive firing of the afferent fibre, a ... ...

Abstract	Ampullary electroreceptor organs of the catfish were apically exposed to 0.3 mM vincristine in order to investigate the part played by the microtubular system in stimulus transduction. The main effects were repetitive firing of the afferent fibre, a reduction of the mean spontaneous activity and a reduction of the spike amplitude two to four days after exposure to vincristine. The mean sensitivity was less susceptible to vincristine than the spontaneous activity. Since the shape of the frequency curves remained unchanged and similar effects as described above were also observed after denervation, we conclude that vincristine most likely does not affect electroreceptor cell functioning, but causes degeneration of the afferent fibre.
MeSH term(s)	Animals ; Antineoplastic Agents, Phytogenic/pharmacology ; Electrophysiology ; Ictaluridae/physiology ; Mechanoreceptors/drug effects ; Mechanoreceptors/physiology ; Nerve Degeneration ; Nerve Fibers/drug effects ; Nerve Fibers/physiology ; Neurons, Afferent/drug effects ; Neurons, Afferent/physiology ; Signal Processing, Computer-Assisted ; Signal Transduction/physiology ; Vincristine/pharmacology
Chemical Substances	Antineoplastic Agents, Phytogenic ; Vincristine (5J49Q6B70F)
Language	English
Publishing date	1996-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	196739-3
ISSN	1873-7544 ; 0306-4522
ISSN (online)	1873-7544
ISSN	0306-4522
DOI	10.1016/0306-4522(95)00555-2
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Article: The effect of hyperosmotic treatment on the functioning of ampullary electroreceptor organs.

Heijmen, P S / Boele, A / Peters, R C

Neuroscience

1996 Volume 72, Issue 4, Page(s) 1107–1115

Abstract: The condition of the microvilli of the apical membrane in ampullary electroreceptor organs of the freshwater catfish, Ictalurus nebulosus, was manipulated by administration of hyperosmotic solutions (500 mM) in order to investigate to what extent the ... ...

Abstract	The condition of the microvilli of the apical membrane in ampullary electroreceptor organs of the freshwater catfish, Ictalurus nebulosus, was manipulated by administration of hyperosmotic solutions (500 mM) in order to investigate to what extent the apical surface area determines the sensitivity and the shape of the frequency curves. Electrophysiological recordings demonstrated that hyperosmotic sucrose and glucose solutions reduce the sensitivity by 80%, and alter the shape of the frequency curves. The synaptic delay and the average spontaneous activity remained almost unchanged. Calculations with an equivalent electric circuit of an electroreceptor cell indicate that a reduction in apical surface area can account for these effects. Apparently, the sensitivity and the frequency curves in ampullary organs in freshwater catfish depend on the apical surface area. A hyperosmotic urea solution proved to be less suitable to investigate the effect of manipulations of the apical surface area. The almost total loss in sensitivity caused by administration of urea is caused by both shunting of the stimulus by leaky tight junctions and a reduced synaptic efficacy. The repetitive activity observed after exposure to glucose or sucrose must be attributed to malfunctioning of postsynaptic parts of the receptor organ.
MeSH term(s)	Animals ; Behavior, Animal/physiology ; Electric Impedance ; Electric Organ/cytology ; Electric Organ/drug effects ; Electric Organ/physiology ; Electrophysiology ; Ictaluridae ; Osmotic Pressure ; Sensitivity and Specificity ; Sensory Receptor Cells/cytology ; Sensory Receptor Cells/drug effects ; Sensory Receptor Cells/physiology ; Skin Physiological Phenomena ; Synapses/physiology ; Urea/metabolism
Chemical Substances	Urea (8W8T17847W)
Language	English
Publishing date	1996-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	196739-3
ISSN	1873-7544 ; 0306-4522
ISSN (online)	1873-7544
ISSN	0306-4522
DOI	10.1016/0306-4522(96)00015-2
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Article: Effects of dietary fats on red blood cell membrane insulin receptor in normo- and hypercholesterolemic miniature swine

Bhathena, S.J / Berlin, E / McClure, D / Peters, R.C

Journal of nutritional biochemistry. Sept 2001. v. 12 (9)

2001

Abstract	It has been demonstrated that the type of dietary fat affects insulin receptors in various tissues in normal humans and animals by altering membrane fluidity. This study compares the effects of n-3 fatty acids from fish oil and n-6 fatty acids from corn oil on red blood cell membrane insulin receptors in normal and hypercholesterolemic minipigs. A group of minipigs were made hypercholesterolemic by feeding cholesterol and lard for 2 months; the other group served as controls and was fed stock diet. Both groups were then fed experimental diets containing either corn oil or menhaden oil or a mixture of the two for 23 additional weeks. Blood was collected at 0, 2, 12 and 23 weeks after the start of the experimental diets and membranes were prepared from the red blood cells. Insulin binding to red blood cell membranes was measured by radioreceptor assay. Plasma insulin was measured by radioimmunoassay. Insulin binding to red blood cell membrane was compared with the fluidity of the membrane measured and reported earlier. There was no significant effect of cholesterol feeding on plasma insulin concentrations. After 23 weeks on experimental diet plasma insulin was significantly higher in minipigs fed menhaden oil compared to those fed corn oil. No such effect was observed in hypercholesterolemic minipigs. No significant effect of either hypercholesterolemia or fish oil was observed on red blood cell insulin binding. A significant negative relationship was observed between insulin binding and anisotropy at 4 degrees C for all probes but at 37 degrees C significant negative relationship was observed only with polar probes. The data suggest that n-3 fatty acids from fish oil significantly increases plasma insulin in minipigs compared to n-6 fatty acids from corn oil. However, the unsaturation has no significant effect on insulin receptors on erythrocytes. Similarly, prior hypercholesterolemic state also has no effect on plasma insulin levels or the insulin binding to red blood cell membranes.
Keywords	dietary fat ; eicosapentaenoic acid ; docosahexaenoic acid ; experimental diets ; menhaden oil ; corn oil ; erythrocytes ; cell membranes ; insulin ; hormone receptors ; cholesterol ; hypercholesterolemia ; miniature swine ; animal models ; omega-3 fatty acids ; omega-6 fatty acids ; binding sites
Language	English
Dates of publication	2001-09
Size	p. 529-535.
Document type	Article
ZDB-ID	1014929-6
ISSN	1873-4847 ; 0955-2863
ISSN (online)	1873-4847
ISSN	0955-2863
Database	NAL-Catalogue (AGRICOLA)

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Article: Simultaneous measurements of calcium mobilization and afferent nerve activity in electroreceptor organs of anesthetized Kryptopterus bicirrhis.

Struik, M L / Steenbergen, H G / Koster, A S / Bretschneider, F / Peters, R C

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

2003 Volume 130, Issue 3, Page(s) 607–613

Abstract: The transduction pathway of ampullary electroreceptor organs involves ionic currents. It has been shown that calcium, as well as sodium and potassium play important parts in this process. In this study we examine the stimulus-evoked changes in the Fura-2 ...

Abstract	The transduction pathway of ampullary electroreceptor organs involves ionic currents. It has been shown that calcium, as well as sodium and potassium play important parts in this process. In this study we examine the stimulus-evoked changes in the Fura-2 ratio in electroreceptor cells. Furthermore, we recorded stimulus-evoked Fura-2 ratio changes while Na+ and K+ channels were blocked by amiloride and TEA. Simultaneously, extracellular recordings of the afferent spike activity were made. The results show the presence of stimulus evoked fluctuations in the Fura-2 ratio. These fluctuations can be abolished by the application of Cd2+, TEA, and amiloride. The stimulus-evoked activity of the afferent nerve was decreased due to application of these drugs. We conclude that the transduction current is carried by Na+, K+, and probably Ca2+. This fits the existing model on transduction in electroreceptors.
MeSH term(s)	Anesthesia ; Animals ; Calcium/metabolism ; Catfishes ; Electric Organ/cytology ; Electric Organ/innervation ; Electric Organ/metabolism ; Electric Stimulation ; Neurons, Afferent/physiology
Chemical Substances	Calcium (SY7Q814VUP)
Language	English
Publishing date	2003-02-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	121246-1
ISSN	1531-4332 ; 1095-6433 ; 0300-9629
ISSN (online)	1531-4332
ISSN	1095-6433 ; 0300-9629
DOI	10.1016/s1095-6433(01)00436-6
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Article: Identification of AMPA receptors in catfish electroreceptor organs.

Andrianov, G N / Peters, R C / Bretschneider, F

Neuroreport

1994 Volume 5, Issue 9, Page(s) 1056–1058

Abstract: Single afferent unit recording in microampullae of the catfish revealed that bath-applied AMPA increases both resting discharge frequency and electrically evoked responses. The potency of AMPA is of the order of 10 microM. DNQX strongly inhibits the ... ...

Abstract	Single afferent unit recording in microampullae of the catfish revealed that bath-applied AMPA increases both resting discharge frequency and electrically evoked responses. The potency of AMPA is of the order of 10 microM. DNQX strongly inhibits the excitatory effects of AMPA. The results suggest the presence of AMPA receptors at the synaptic membrane of ampullary electroreceptor organs in the catfish.
MeSH term(s)	Action Potentials/physiology ; Animals ; Electric Stimulation ; Ictaluridae/metabolism ; Mechanoreceptors/drug effects ; Mechanoreceptors/physiology ; Nerve Fibers/drug effects ; Nerve Fibers/physiology ; Neurons, Afferent/drug effects ; Neurons, Afferent/physiology ; Quinoxalines/pharmacology ; Receptors, AMPA/drug effects ; Receptors, AMPA/metabolism ; Receptors, Glutamate/drug effects ; Receptors, Glutamate/physiology ; Synapses/physiology ; Synaptic Membranes/drug effects ; Synaptic Membranes/physiology ; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/pharmacology
Chemical Substances	Quinoxalines ; Receptors, AMPA ; Receptors, Glutamate ; FG 9041 (62T278S1MX) ; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (77521-29-0)
Language	English
Publishing date	1994-05-09
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1049746-8
ISSN	1473-558X ; 0959-4965
ISSN (online)	1473-558X
ISSN	0959-4965
DOI	10.1097/00001756-199405000-00010
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Article: Collagen gels populated with rat neonatal heart cells can be used for optical recording of rhythmic contractions which also show ECG-like potentials.

Souren, J E / Peters, R C / Van Wijk, R

Experientia

1994 Volume 50, Issue 8, Page(s) 712–716

Abstract: Neonatal heart cells populated collagen gels show rhythmic contractions which can be recorded optically and electrically. Optical recordings revealed two populations of rhythmically contracting gels: 1) highly coherent contracting collagen gels with ... ...

Abstract	Neonatal heart cells populated collagen gels show rhythmic contractions which can be recorded optically and electrically. Optical recordings revealed two populations of rhythmically contracting gels: 1) highly coherent contracting collagen gels with normally distributed contraction interval times and contraction amplitudes, and 2) irregularly contracting gels with a multi-modal distribution of contraction interval times and amplitudes. The irregularly contracting gels were shown to be 'semi regular', which means that a short contraction interval was preferentially followed by another short interval. The volume of the collagen gel during the contraction decreased, and our calculations indicate that the myocytes expel 3-10 times their own volume from the gel. Changes in electrical potential were observed depending on the location of the electrodes. These electrical, ECG-like changes in potential were maximal when one electrode was placed in the centre and the other at the edge of the gel. The results of this study indicate that myocyte-populated collagen gels are a very promising system for studies of electrophysiology and coherent contractions.
MeSH term(s)	Animals ; Animals, Newborn ; Cells, Cultured ; Collagen ; Densitometry/instrumentation ; Densitometry/methods ; Electrocardiography ; Gels ; Heart/physiology ; Microscopy, Phase-Contrast/instrumentation ; Microscopy, Phase-Contrast/methods ; Myocardial Contraction ; Rats ; Time Factors
Chemical Substances	Gels ; Collagen (9007-34-5)
Language	English
Publishing date	1994-08-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1972-0
ISSN	0014-4754
ISSN	0014-4754
DOI	10.1007/bf01919368
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