Article ; Online: Cell Fractionation.
Methods in molecular biology (Clifton, N.J.)
2023 Volume 2715, Page(s) 65–71
Abstract: Protein function is generally dependent on its subcellular localization. In gram-negative bacteria such as Escherichia coli, a protein can be targeted to five different compartments: the cytoplasm, the inner membrane, the periplasm, the outer membrane, ... ...
| Abstract | Protein function is generally dependent on its subcellular localization. In gram-negative bacteria such as Escherichia coli, a protein can be targeted to five different compartments: the cytoplasm, the inner membrane, the periplasm, the outer membrane, and the extracellular medium. Different approaches can be used to determine the protein localization within cell such as in silico identification of protein signal sequences and motifs, electron microscopy and immunogold labeling, optical fluorescence microscopy, and biochemical technics. In this chapter, we describe a simple and efficient method to isolate the different compartments of Escherichia coli by a fractionation method and to determine the presence of the protein of interest. For inner membrane proteins, we propose a method to discriminate between integral and peripheral membrane proteins. |
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| MeSH term(s) | Cell Fractionation ; Chemical Fractionation ; Cytoplasm ; Escherichia coli ; Membrane Proteins |
| Chemical Substances | Membrane Proteins |
| Language | English |
| Publishing date | 2023-11-06 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ISSN | 1940-6029 |
| ISSN (online) | 1940-6029 |
| DOI | 10.1007/978-1-0716-3445-5_3 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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