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Article: Immunogenicity of the hydrophilic region of the MUC1 mucin protein core.

Price, M / Petrakou, E / Sekowski, M / Murray, A

2011 Volume 4, Issue 2, Page(s) 337–339

Abstract: This report is an analysis of data relating to the epitopes of 28 murine monoclonal antibodies reactive with the protein core of human carcinoma-associated MUC1 mucins. All anti-MUC1 antibodies define epitopes of linear sequences of 3, 4 or 5 amino acids ...

Abstract	This report is an analysis of data relating to the epitopes of 28 murine monoclonal antibodies reactive with the protein core of human carcinoma-associated MUC1 mucins. All anti-MUC1 antibodies define epitopes of linear sequences of 3, 4 or 5 amino acids within the hydrophilic domain, APDTRPAP, which is expressed multiple times in a highly conserved 20 amino acid repeat sequence of the MUC1 core. The R residue is present in the epitopes defined by all of the 28 anti-MUC1 monoclonal antibodies. Epitopes of antibodies originally prepared against immunogens containing human milk fat globule membranes include the motif DTR in over 90% of the examples studied.
Language	English
Publishing date	2011-05-13
Publishing country	Greece
Document type	Journal Article
ZDB-ID	1222484-4
ISSN	1791-2431 ; 1021-335X
ISSN (online)	1791-2431
ISSN	1021-335X
DOI	10.3892/or.4.2.337
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Zs.A 4213: Show issues

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Article ; Online: Quantiferon-Cytomegalovirus assay: A potentially useful tool in the evaluation of CMV-specific CD8+ T-cell reconstitution in pediatric hematopoietic stem cell transplant patients.

Paouri, Bilio / Soldatou, Alexandra / Petrakou, Eftihia / Theodosaki, Maria / Tsentidis, Charalampos / Kaisari, Katerina / Oikonomopoulou, Christina / Matsas, Minos / Goussetis, Eugenios

Pediatric transplantation

2018 Volume 22, Issue 5, Page(s) e13220

Abstract: Pediatric HSCT recipients are at high risk for CMV reactivation due to their immature immune system and therapy following transplantation. Reconstitution of CMV-specific T-cell immunity is associated with control and protection against CMV. The clinical ... ...

Abstract	Pediatric HSCT recipients are at high risk for CMV reactivation due to their immature immune system and therapy following transplantation. Reconstitution of CMV-specific T-cell immunity is associated with control and protection against CMV. The clinical utility of monitoring CMV-specific CMI to predict CMV viremia in pediatric HSCT patients using the Quantiferon-CMV (QIAGEN
MeSH term(s)	Adolescent ; CD8-Positive T-Lymphocytes/immunology ; Child ; Child, Preschool ; Cytomegalovirus/immunology ; Cytomegalovirus/isolation & purification ; Cytomegalovirus Infections/diagnosis ; Cytomegalovirus Infections/immunology ; Female ; Hematopoietic Stem Cell Transplantation/adverse effects ; Humans ; Immunity, Cellular ; Infant ; Longitudinal Studies ; Male ; Prospective Studies ; Real-Time Polymerase Chain Reaction ; Viremia/diagnosis ; Viremia/immunology
Language	English
Publishing date	2018-05-18
Publishing country	Denmark
Document type	Clinical Trial ; Journal Article
ZDB-ID	1390284-2
ISSN	1399-3046 ; 1397-3142
ISSN (online)	1399-3046
ISSN	1397-3142
DOI	10.1111/petr.13220
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Zs.A 4947: Show issues

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Article ; Online: HLA-matched sibling stem cell transplantation in children with β-thalassemia with anti-thymocyte globulin as part of the preparative regimen: the Greek experience.

Goussetis, E / Peristeri, I / Kitra, V / Vessalas, G / Paisiou, A / Theodosaki, M / Petrakou, E / Dimopoulou, M N / Graphakos, S

Bone marrow transplantation

2012 Volume 47, Issue 8, Page(s) 1061–1066

Abstract: BU combined with CY, the preferred preparatory regimen for thalassemic patients, is associated with a substantial incidence of graft rejection especially in patients with advanced disease stage. This study retrospectively analyzes the outcome of 75 ... ...

Abstract	BU combined with CY, the preferred preparatory regimen for thalassemic patients, is associated with a substantial incidence of graft rejection especially in patients with advanced disease stage. This study retrospectively analyzes the outcome of 75 consecutive pediatric patients with β-thalassemia who underwent HLA-matched sibling transplantation after anti-thymocyte globulin (ATG)-containing myeloablative conditioning regimens. With a median follow-up of 9 years (range 1-15 years), the overall survival (OS) and thalassemia free survival (TFS) rates were 96% and 92%, respectively. Both the estimated TRM and the cumulative incidence of rejection/failure were 4%. The cumulative incidences of acute GVHD grade II-III and grade III were 20% and 5.3%, respectively. No patient developed acute GVHD grade IV. Only two patients developed extensive chronic GVHD. The estimated OS and TFS for patients with Class 1 and 2 disease according to Pesaro criteria were 96.3% and 94.4%, whereas for patients with Class 3 disease they were 94.1% and 88.2%, respectively. In our series, the use of myeloablative conditioning regimens, which include ATG for the transplantation of thalassemic children from matched sibling donors, resulted in excellent outcomes with very low incidences of TRM and rejection.
MeSH term(s)	Adolescent ; Antilymphocyte Serum/administration & dosage ; Child ; Child, Preschool ; Disease-Free Survival ; Female ; Follow-Up Studies ; Graft Rejection/etiology ; Graft Rejection/mortality ; Graft Rejection/therapy ; Graft vs Host Disease/etiology ; Graft vs Host Disease/mortality ; Graft vs Host Disease/therapy ; Greece/epidemiology ; Histocompatibility Testing ; Humans ; Immunosuppressive Agents/administration & dosage ; Infant ; Living Donors ; Male ; Retrospective Studies ; Siblings ; Stem Cell Transplantation ; Survival Rate ; Transplantation Conditioning/methods ; Transplantation, Homologous ; beta-Thalassemia/mortality ; beta-Thalassemia/therapy
Chemical Substances	Antilymphocyte Serum ; Immunosuppressive Agents
Language	English
Publishing date	2012-08
Publishing country	England
Document type	Journal Article
ZDB-ID	632854-4
ISSN	1476-5365 ; 0268-3369 ; 0951-3078
ISSN (online)	1476-5365
ISSN	0268-3369 ; 0951-3078
DOI	10.1038/bmt.2011.219
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Zs.A 2168: Show issues

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Article: Effects of air pollution and smoking on DNA damage of human lymphocytes.

Piperakis, S M / Petrakou, E / Tsilimigaki, S

Environmental and molecular mutagenesis

2000 Volume 36, Issue 3, Page(s) 243–249

Abstract: The comet assay is a useful technique for the study of genetic damage in humans exposed to environmental mutagens and carcinogens. In this study the effects of hydrogen peroxide (H(2)O(2)) and ultraviolet (UV) irradiation on 80 healthy individuals living ...

Abstract	The comet assay is a useful technique for the study of genetic damage in humans exposed to environmental mutagens and carcinogens. In this study the effects of hydrogen peroxide (H(2)O(2)) and ultraviolet (UV) irradiation on 80 healthy individuals living in urban and rural areas with different smoking habits were investigated. Endonuclease III (endo III) treatment was also used to reveal the level of oxidized pyrimidine formation in these groups. The extent of damage and subsequent repair appear to be influenced by the living conditions (urban or rural areas). Smoking, however, was shown to have the most significant effect on DNA damage on all groups studied.
MeSH term(s)	Adult ; Air Pollution ; Cells, Cultured ; Comet Assay ; Cryopreservation ; DNA Damage ; DNA Repair ; Greece ; Humans ; Hydrogen Peroxide/toxicity ; Lymphocytes/cytology ; Lymphocytes/drug effects ; Lymphocytes/radiation effects ; Male ; Middle Aged ; Rural Population ; Smoking ; Ultraviolet Rays ; Urban Population
Chemical Substances	Hydrogen Peroxide (BBX060AN9V)
Language	English
Publishing date	2000
Publishing country	United States
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639145-x
ISSN	1098-2280 ; 0893-6692
ISSN (online)	1098-2280
ISSN	0893-6692
DOI	10.1002/1098-2280(2000)36:3<243::aid-em8>3.0.co;2-9
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Zs.B 2404: Show issues

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Article: Interleukin-8 and monocyte chemotactic protein-1 mRNA expression in perinatally infected and asphyxiated preterm neonates.

Petrakou, E / Mouchtouri, A / Levi, E / Lipsou, N / Xanthou, M / Fotopoulos, S

Neonatology

2007 Volume 91, Issue 2, Page(s) 107–113

Abstract: Background: Inflammation due to perinatal infection (PI) and perinatal asphyxia (PA) may cause damage to various tissues and very often to the immature brain of the fetus and the newborn. Previously, we have shown that the neonatal immune system has the ...

Abstract	Background: Inflammation due to perinatal infection (PI) and perinatal asphyxia (PA) may cause damage to various tissues and very often to the immature brain of the fetus and the newborn. Previously, we have shown that the neonatal immune system has the ability to produce increased chemokine protein levels in the serum during the inflammatory response caused by PI and PA. Aim: The aim of our present study was to investigate mRNA levels of the proinflammatory chemokines interleukin-8 (IL-8) and monocyte chemotactic protein-1 (MCP-1) in peripheral blood leukocytes from infected and asphyxiated neonates. Methods: Forty-two premature neonates were studied; 11 with PI, 16 with PA and 15 without PA and PI, were used as controls. IL-8 and MCP-1 mRNA levels were investigated in whole blood and in phytohemagglutinin-activated lymphocytes using semi-quantitative polymerase chain reaction and real-time polymerase chain reaction, respectively. Results: IL-8 mRNA levels were significantly increased in whole blood both during PA and PI, while MCP-1 mRNA levels were not. In vitro activated lymphocytes expressed significantly increased IL-8 mRNA levels during PI, whereas no increase was observed during PA. MCP-1 mRNA levels were significantly increased in activated lymphocytes during PA, while no increase was observed during PI. Conclusions: Our data show that chemokine mRNA levels expressed by activated lymphocytes during inflammation caused by PIs are different to those expressed during PAs. These findings might have important implications during the administration of specific chemokine antagonists in order to prevent or reduce tissue damage caused by inflammation.
MeSH term(s)	Asphyxia Neonatorum/blood ; Chemokine CCL2/biosynthesis ; Chemokine CCL2/blood ; Chemokine CCL2/genetics ; Cross Infection/blood ; Gene Expression ; Humans ; Infant, Newborn ; Interleukin-8/biosynthesis ; Interleukin-8/blood ; Interleukin-8/genetics ; Leukocytes, Mononuclear/drug effects ; Leukocytes, Mononuclear/metabolism ; Lymphocyte Activation ; Mitogens/pharmacology ; Phytohemagglutinins/pharmacology ; RNA, Messenger/metabolism
Chemical Substances	Chemokine CCL2 ; Interleukin-8 ; Mitogens ; Phytohemagglutinins ; RNA, Messenger
Language	English
Publishing date	2007
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2266911-5
ISSN	1661-7819 ; 1661-7800
ISSN (online)	1661-7819
ISSN	1661-7800
DOI	10.1159/000097127
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Zs.A 391: Show issues

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Article: Epitope mapping of anti-MUC1 mucin protein core monoclonal antibodies.

Petrakou, E / Murray, A / Price, M R

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

1996 Volume 19 Suppl 1, Page(s) 21–29

Abstract: A panel of 56 murine monoclonal antibodies against the MUC1 mucin was analysed for reactivity against purified MUC1 mucin and synthetic MUC1 mucin protein core-related peptides. In an ELISA assay, with purified normal urinary mucin as the target antigen, ...

Abstract	A panel of 56 murine monoclonal antibodies against the MUC1 mucin was analysed for reactivity against purified MUC1 mucin and synthetic MUC1 mucin protein core-related peptides. In an ELISA assay, with purified normal urinary mucin as the target antigen, 48/56 (86%) of antibodies showed positive reactivity. A smaller proportion of antibodies, 31/56 (55%), reacted with a bovine serum albumin conjugate containing the synthetic peptide, APDTRPAPG. This peptide represents the hydrophilic region of the tandem repeat sequence of the MUC1 mucin core. These 31 anti-mucin core antibodies were then evaluated for reactivity with a set of overlapping heptamers with sequences based upon that of the 20-amino acid tandem repeat of the MUC1 protein core. For this purpose, the 'Pepscan' procedure was employed to detect antibody binding to peptides tethered at their carboxy termini to the polypropylene pins. Synthetic peptides binding to these antibodies were identified for 29 of the samples provided and epitopes or 'minimum binding units' were deduced for these. Most antibodies bound to epitopes of 4 amino acid residues with the sequence RPAP occurring most frequently. The results confirm that the short hydrophilic region PDTRPAP in the MUC1 mucin core is immunodominant in the induction of antibodies to the MUC1 mucin since half of the antibodies submitted reacted with this peptide.
MeSH term(s)	Amino Acid Sequence ; Animals ; Antibodies, Monoclonal/analysis ; Antibody Specificity/immunology ; Cattle ; Enzyme-Linked Immunosorbent Assay ; Epitope Mapping ; Humans ; Immunodominant Epitopes/immunology ; Mice ; Molecular Sequence Data ; Mucin-1/immunology ; Peptide Fragments/immunology
Chemical Substances	Antibodies, Monoclonal ; Immunodominant Epitopes ; Mucin-1 ; Peptide Fragments
Language	English
Publishing date	1996-04-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605825-5
ISSN	1423-0380 ; 1010-4283 ; 0289-5447
ISSN (online)	1423-0380
ISSN	1010-4283 ; 0289-5447
DOI	10.1159/000056501
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Zs.A 1598: Show issues

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Article ; Online: Mesenchymal derivatives of genetically unstable human embryonic stem cells are maintained unstable but undergo senescence in culture as do bone marrow-derived mesenchymal stem cells.

Karagiannidou, Angeliki / Varela, Ioanna / Giannikou, Krinio / Tzetis, Maria / Spyropoulos, Antonia / Paterakis, George / Petrakou, Eftichia / Theodosaki, Maria / Goussetis, Evgenios / Kanavakis, Emmanuel

Cellular reprogramming

2014 Volume 16, Issue 1, Page(s) 1–8

Abstract: Recurrent chromosomal alterations have been repeatedly reported in cultured human embryonic stem cells (hESCs). The effects of these alterations on the capability of pluripotent cells to differentiate and on growth potential of their specific ... ...

Abstract	Recurrent chromosomal alterations have been repeatedly reported in cultured human embryonic stem cells (hESCs). The effects of these alterations on the capability of pluripotent cells to differentiate and on growth potential of their specific differentiated derivatives remain unclear. Here, we report that the hESC lines HUES-7 and -9 carrying multiple chromosomal alterations produce in vitro mesenchymal stem cells (MSCs) that show progressive growth arrest and enter senescence after 15 and 16 passages, respectively. There was no difference in their proliferative potential when compared with bone marrow-derived MSCs. Array comparative genomic hybridization analysis (aCGH) of hESCs and their mesenchymal derivatives revealed no significant differences in chromosomal alterations, suggesting that genetically altered hESCs are not selected out during differentiation. Our findings indicate that genetically unstable hESCs maintain their capacity to differentiate in vitro into MSCs, which exhibit an in vitro growth pattern of normal MSCs and not that of transformed cells.
MeSH term(s)	Bone Marrow Cells/cytology ; Bone Marrow Cells/metabolism ; Cell Line ; Cellular Senescence/genetics ; Child ; Comparative Genomic Hybridization ; Embryonic Stem Cells/cytology ; Embryonic Stem Cells/metabolism ; Female ; Genomic Instability ; Humans ; Male ; Mesenchymal Stromal Cells/cytology ; Mesenchymal Stromal Cells/metabolism
Language	English
Publishing date	2014-02
Publishing country	United States
Document type	Clinical Trial ; Journal Article
ZDB-ID	2542436-1
ISSN	2152-4998 ; 1557-7457 ; 2152-4971
ISSN (online)	2152-4998 ; 1557-7457
ISSN	2152-4971
DOI	10.1089/cell.2013.0040
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Zs.A 5452: Show issues

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Article ; Online: Myofibroblasts generated in culture from sclerotic skin lesions of a patient with extensive chronic graft-versus-host disease after allogeneic hematopoietic stem cell transplantation are of recipient origin.

Goussetis, Evgenios / Spiropoulos, Antonia / Theodosaki, Maria / Stefanaki, Kalliopi / Petrakou, Eftichia / Graphakos, Stelios

Stem cells and development

2010 Volume 19, Issue 9, Page(s) 1285–1287

Abstract: Abstract The origin (recipient/donor) of the myofibroblasts mediating fibrosis in sclerodermatous chronic graft-versus-host disease (cGvHD) was investigated. Sclerodermatous specimens obtained from a patient with extensive cGvHD after an HLA-identical ... ...

Abstract	Abstract The origin (recipient/donor) of the myofibroblasts mediating fibrosis in sclerodermatous chronic graft-versus-host disease (cGvHD) was investigated. Sclerodermatous specimens obtained from a patient with extensive cGvHD after an HLA-identical sibling bone marrow transplantation were cultured in order to derive tissue myofibroblasts. All proliferating a-SMA+ fibroblastoid cells revealed recipient origin as examined by variable number tandem repeat (VNTR)-PCR. This case report shows that fibrosis in sclerodermatous lesions results from the activation and proliferation of locally-derived recipient fibroblasts rather than from donor-derived fibroblasts or circulating fibrocytes.
MeSH term(s)	Cell Culture Techniques ; Cell Proliferation ; Cells, Cultured ; Chronic Disease ; Graft vs Host Disease/complications ; Graft vs Host Disease/etiology ; Graft vs Host Disease/immunology ; Graft vs Host Disease/pathology ; Hematopoietic Stem Cell Transplantation/adverse effects ; Humans ; Myofibroblasts/pathology ; Myofibroblasts/physiology ; Sclerosis ; Siblings ; Skin Diseases/etiology ; Skin Diseases/immunology ; Skin Diseases/pathology ; Tissue Donors ; Transplantation, Homologous
Language	English
Publishing date	2010-09
Publishing country	United States
Document type	Case Reports ; Letter
ZDB-ID	2142214-X
ISSN	1557-8534 ; 1547-3287
ISSN (online)	1557-8534
ISSN	1547-3287
DOI	10.1089/scd.2009.0401
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Zs.A 3616: Show issues

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Article: Nicotinamide "protects" resting lymphocytes exposed to hydrogen peroxide from necrosis but not from apoptosis.

Tronov, V A / Konstantinov, E M / Petrakou, E / Tsilimigaki, S / Piperakis, S M

Cell biology and toxicology

2002 Volume 18, Issue 6, Page(s) 359–367

Abstract: The aim of this work was to investigate the relationship between mechanisms of DNA repair and apoptosis induced by oxidative stress (H2O2) in human lymphocytes. Using the comet assay, fluorescent microscopy, and DNA electrophoresis, we studied the DNA ... ...

Abstract	The aim of this work was to investigate the relationship between mechanisms of DNA repair and apoptosis induced by oxidative stress (H2O2) in human lymphocytes. Using the comet assay, fluorescent microscopy, and DNA electrophoresis, we studied the DNA damage induced by hydrogen peroxide (H2O2) treatment, the time and the amount of repair of strand breaks, the type of cell death, and the influence of inhibitors of repair (nicotinamide). When lymphocytes were treated with H2O2, we observed an increased in necrosis compared to apoptosis. However, when nicotinamide (which inhibits DNA repair) was added, the mode of death reversed to increased apoptosis. These results indicate that nicotinamide "protects" resting lymphocytes exposed to H2O2 from necrosis but not from apoptosis.
MeSH term(s)	Apoptosis/drug effects ; Cell Survival/drug effects ; Comet Assay ; DNA Damage/drug effects ; DNA Fragmentation/drug effects ; DNA Repair/drug effects ; Electrophoresis, Agar Gel ; Humans ; Hydrogen Peroxide/toxicity ; Lymphocytes/drug effects ; Lymphocytes/pathology ; Necrosis ; Niacinamide/pharmacology
Chemical Substances	Niacinamide (25X51I8RD4) ; Hydrogen Peroxide (BBX060AN9V)
Language	English
Publishing date	2002-12-13
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	48824-0
ISSN	1573-6822 ; 0742-2091
ISSN (online)	1573-6822
ISSN	0742-2091
DOI	10.1023/a:1020859405262
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Zs.B 3024: Show issues

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Article ; Online: Activin-A exerts a crucial anti-inflammatory role in neonatal infections.

Petrakou, Eutichia / Fotopoulos, Spyros / Anagnostakou, Marina / Anatolitou, Fani / Samitas, Konstantinos / Semitekolou, Maria / Xanthou, Georgina / Xanthou, Marietta

Pediatric research

2013 Volume 74, Issue 6, Page(s) 675–681

Abstract: Background: Activin-A is a cytokine with a critical role in infections and associated inflammation in experimental models and humans. Still, the effects of activin-A on neonatal infections remain elusive. Here, we investigated the expression of activin- ... ...

Abstract	Background: Activin-A is a cytokine with a critical role in infections and associated inflammation in experimental models and humans. Still, the effects of activin-A on neonatal infections remain elusive. Here, we investigated the expression of activin-A in the serum of septicemic preterm and term neonates and in peripheral blood leukocytes stimulated with inflammatory agents in vitro. The role of activin-A in the regulation of inflammatory responses by neonatal leukocytes was delineated. Methods: Peripheral blood was obtained from 37 septicemic neonates between the first and fifth days postinfection and from 35 healthy controls. Isolated monocytes and lymphocytes were stimulated with lipopolysaccharide (LPS) or phytohemagglutinin (PHA) in vitro in the presence of activin-A. Cell proliferation, cytokine, and chemokine release were investigated. Results: Activin-A was significantly increased in the serum of preterm septicemic neonates. Neonatal leukocytes secreted copious amounts of activin-A following stimulation, pointing to these cells as an essential source of activin-A in the circulation. Of note, treatment of neonatal leukocytes with activin-A during PHA and LPS stimulation resulted in significantly decreased interleukin (IL)-1β, IL-6, and CXCL8 production, concomitant with a striking increase in the anti-inflammatory mediator, IL-10. Conclusion: Our findings uncover activin-A as a novel immunomodulatory agent critical for the control of inflammatory responses in septicemic neonates.
MeSH term(s)	Activins/physiology ; Case-Control Studies ; Chemokines/secretion ; Cytokines/secretion ; Humans ; Infant, Newborn ; Infant, Newborn, Diseases/physiopathology ; Infant, Premature ; Infection/physiopathology ; Inflammation/prevention & control ; Monocytes/immunology ; Monocytes/secretion
Chemical Substances	Chemokines ; Cytokines ; activin A ; Activins (104625-48-1)
Language	English
Publishing date	2013-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	4411-8
ISSN	1530-0447 ; 0031-3998
ISSN (online)	1530-0447
ISSN	0031-3998
DOI	10.1038/pr.2013.159
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Zs.A 564: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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