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  1. Article ; Online: Detection and molecular characterization of Polymyxa betae, transmitting agent of sugar beet rhizomania disease, in Iran

    Fatemeh Hassanzadeh Davarani / Saeed Rezaee / Seyed B. Mahmoudi / Peyman Norouzi / Mohammad R. Safarnejad / Hossein Safarpour

    Spanish Journal of Agricultural Research, Vol 12, Iss 3, Pp 787-

    2014  Volume 794

    Abstract: The plasmodiophorid Polymyxa betae is considered as the only natural transmitting agent of Beet necrotic yellow vein virus (BNYVV), the most devastating agent of sugar beet fields throughout the world. To evaluate for the first time the genetic diversity ...

    Abstract The plasmodiophorid Polymyxa betae is considered as the only natural transmitting agent of Beet necrotic yellow vein virus (BNYVV), the most devastating agent of sugar beet fields throughout the world. To evaluate for the first time the genetic diversity of P. betae isolated from different autumn and spring sugar beet fields, and also to detect the presence of virus in these isolates, susceptible sugar beet plants (cv. Regina) were grown in soil samples collected from 10 different regions of Iran. P. betae detection was carried out using root microscopic observations, DAS-ELISA, and PCR-based methods. Results showed the presence of plasmodiophorids in all soil samples. Complementary assays also revealed the presence of viruses in soil samples collected from Khorasan Razavi, Fars, Hamadan and Kermanshah regions. The genetic diversity was evaluated through comparing glutathione-S-transferase nucleotide sequences amplified by PCR with the Internal Transcribed Spacers region. Results showed no significant differences in nucleotide sequences between virus-bearing and virus-free isolates of P. betae .
    Keywords Beta vulgaris ; cystosori ; ITS ; GST ; BNYVV ; virus-bearing ; virus-free ; Agriculture ; S
    Language English
    Publishing date 2014-07-01T00:00:00Z
    Publisher Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Screening of Sugar Beet Tissue Culture Clones for Resistance to Rhizomania Disease

    Vahid Salari / Peyman Norouzi / Mansour Omidi / Reza Amiri / Iman Zandieh

    Pakistan Journal of Biological Sciences, Vol 11, Iss 12, Pp 1610-

    2008  Volume 1614

    Abstract: In this study, sugar beet tissue culture clones were used to screen rhizomania resistant genotypes. At first, explants derived from shoot tips of sugar beet seedlings were transferred to shoot tip elongation media after surface sterilization. Then, the ... ...

    Abstract In this study, sugar beet tissue culture clones were used to screen rhizomania resistant genotypes. At first, explants derived from shoot tips of sugar beet seedlings were transferred to shoot tip elongation media after surface sterilization. Then, the grown shoots were transferred to media containing various hormonal combinations NAA, BA, IBA and GA 3 for multiplication, growth and rooting. Later, the clones were transferred to soil-peatmoss mixture were adapted to greenhouse conditions. For screening clones against rhizomania, the genotypes of adapted clones were selected and inoculated to rhizomania-infested soil. This experiment was in a randomized complete block design with three replicates (three inoculation times) in greenhouse. Adapted plants were transferred to the soil containing rhizomania virus. All infested soils were diluted 3 to 7 with sand. After two months, infested plants were examined by DAS-ELISA test also optical densities of the samples were analyzed by SAS program. Significant differences among genotypes and blocks were observed. Genotypes were classified to few groups (ranked from completely susceptible to completely resistant). The difference between blocks was because of difference of inoculation time temperature. Use of clones of each genotype caused an increase in selection accuracy of resistant genotypes. By use of this method, chance of escaping from inoculation factor decrease and researchers can determine to be resistance of plants with high level of confidence and apply in breeding programs.
    Keywords Sugar beet ; rhizomania ; tissue culture clone ; ELISA ; Biology (General) ; QH301-705.5 ; Science ; Q ; DOAJ:Biology ; DOAJ:Biology and Life Sciences
    Subject code 630
    Language English
    Publishing date 2008-01-01T00:00:00Z
    Publisher Asian Network for Scientific Information
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods

    Seyed Mohammad Amin Feghhi, Peyman Norouzi , Abbas Saidi, Katayoun Zamani and Reza Amiri

    Electronic Journal of Plant Breeding, Vol 3, Iss 1, Pp 598-

    2012  Volume 605

    Abstract: Rhizomania, caused by Beet necrotic yellow vein virus (BNYVV), is the most economically important diseases of sugar beet inIran. We have identified molecular markers associated with resistance gene(s) to this disease. A F2 population including of ... ...

    Abstract Rhizomania, caused by Beet necrotic yellow vein virus (BNYVV), is the most economically important diseases of sugar beet inIran. We have identified molecular markers associated with resistance gene(s) to this disease. A F2 population including of 106individuals developed at the Sugar Beet Seed Institute, Karaj, Iran, was used to identify molecular markers associated withrhizomania resistance gene from the Holly source. In this study, we used pair combinations of single RAPD primers in bulkedsegregant analysis (BSA) of two bulks (resistant and susceptible) and F2 population individuals. Accordingly 397 paircombinations of single RAPD primers were used. However, nine primer pairs showed polymorphism between DNA bulks. Thepolymorphic markers were tested among the individual plants of the two susceptible and resistant bulks and further were used toanalyze the F2 individuals. Finally, the markers distance from the resistance gene was estimated by using both the Map makerver.3.0 and the frequency of recombinant plants method. Using frequency of recombinant plants method to tag Rz1 gene, it wasfound that R1, R2 and R3 markers were 2.3, 8.3 and 16.6 cM apart in repulsion phase and C3, C4, C1, C2, C5, and C6 markers were20, 21.4, 27.5, 32.9, 43.7, and 51.9 cM apart in coupling phase, respectively. On the other hand, tagging Rz1 gene by Mapmaker ver.3.0 method showed that R1, R2 and R3 markers were 32.4, 44.5 and 60.1 cM apart in repulsion phase and C3, C2, C4and C1 markers were 25, 34.7, 46.5 and 57.9 cM apart in coupling phase, respectively. After sequencing the products amplifiedby MF1 and MF2 (Operon primers number have been replaced by these characters) primer pairs, new PCR primers were used togenerate the SCAR marker R1 (this primer sequence is under patent processing and will be shared once it gets patented) whichcan be readily used for marker assisted selection in breeding programmes.
    Keywords Sugar beet ; Rhizomania disease ; RAPD marker ; primer pairs ; Rz1 gene ; Agriculture (General) ; S1-972 ; Agriculture ; S ; DOAJ:Agriculture (General) ; DOAJ:Agriculture and Food Sciences ; Plant culture ; SB1-1110 ; DOAJ:Plant Sciences
    Subject code 630
    Language English
    Publishing date 2012-03-01T00:00:00Z
    Publisher Indian Society of Plant Breeders
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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