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  1. Book ; Thesis: Untersuchungen zur Dynamik des Vorkommens und zur Persistenz Cephalosporin- oder Fluorchinolon-resistenter Escherichia coli-Stämme in der Mastschweineproduktion

    Pfeifer, Nicola

    (Édition scientifique)

    2023  

    Institution Justus-Liebig-Universität Gießen
    Author's details eingereicht von Nicola Maria Pfeifer
    Series title Édition scientifique
    Language German
    Size VI, 259 Seiten, Illustrationen, Diagramme, 21.5 cm x 15 cm, 350 g
    Edition 1. Auflage
    Publisher VVB LAUFERSWEILER Verlag
    Publishing place Gießen
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Dissertation, Justus-Liebig-Universität Gießen, 2023
    HBZ-ID HT030009205
    ISBN 978-3-8359-7094-6 ; 3-8359-7094-1
    Database Catalogue ZB MED Nutrition, Environment, Agriculture

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    In stock of ZB MED Bonn / Germany

    Shelf markLoan statusLocation
    228/93 available for loan (reading room) Freihandmagazin (U1)

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  2. Book: Untersuchungen zur Dynamik des Vorkommens und zur Persistenz Cephalosporin- oder Fluorchinolon-resistenter Escherichia coli-Stämme in der Mastschweineproduktion

    Pfeifer, Nicola Maria

    (Edition Scientifique)

    2023  

    Abstract: Ziel dieser Studie war die Bestimmung der Prävalenz Cephalosporin- bzw. Fluorchinolon-resistenter Gammaproteobakterien auf vier thüringischen Betrieben über einen längeren Zeitraum. Dabei wurden Informationen zu den beprobten Tiergruppen und zu ... ...

    Series title Edition Scientifique
    Abstract Ziel dieser Studie war die Bestimmung der Prävalenz Cephalosporin- bzw. Fluorchinolon-resistenter Gammaproteobakterien auf vier thüringischen Betrieben über einen längeren Zeitraum. Dabei wurden Informationen zu den beprobten Tiergruppen und zu antibiotischen Therapien gesammelt sowie als Indikatororganismen dienende Cephalosporin- bzw. Fluorchinolon-resistente E. coli-Stämme isoliert und charakterisiert. Aus den gewonnenen Daten sollte die mögliche Persistenz einzelner Stämme nachgewiesen u...
    Keywords E-Coli ; Ecoli ; Escherichia ; Schwein ; Mast
    Language German
    Size 276 p.
    Publisher VVB Laufersweiler Verlag
    Document type Book
    Note Ernährungs-, Umwelt- und Agrarwissenschaften 250418
    Format 150 x 215
    ISBN 9783835970946 ; 3835970941
    Database PDA

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  3. Book ; Audio / Video ; Online: Identifying persistent quinolone and cephalosporin resistant Escherichia coli strains along the pork production chain in Thuringian pigsties

    Pfeifer, Nicola / Weber, Michael / Barth, Stefanie / Berens, Christian / Menge, Christian

    2023  

    Keywords Text ; ddc:630
    Language English
    Publishing date 2023-05-23
    Publishing country de
    Document type Book ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Audio / Video ; Online: Antimicrobial resistance – development and persistence of resistomes and coding genetic elements in pig farming

    Pfeifer, Nicola* / Wiegand, Elisabeth

    2019  

    Keywords Text ; abstract_or_summary ; ddc:570
    Language English
    Publishing date 2019-09-27
    Publishing country de
    Document type Article ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Book ; Audio / Video ; Online: Dynamics of quinolone- and cephalosporin-resistant Escherichia coli carriage along the production chain in Thuringian pigsties

    Pfeifer, Nicola / Barth, Stefanie / Berens, Christian / Menge, Christian

    2021  

    Keywords Text ; ddc:570
    Language English
    Publishing date 2021-06-15
    Publishing country de
    Document type Book ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Audio / Video ; Online: Antibiotikaresistenzen - Entwicklung und Persistenz von Resistomen und kodierenden genetischen Elementen in der Schweinehaltung ; Antimicrobial resistance – development and persistence of resistomes and coding genetic elements in pig farming

    Pfeifer, Nicola* / Wiegand, Elisabeth / Berens, Christian / Menge, Christian

    2020  

    Keywords Text ; abstract_or_summary ; ddc:570
    Language English
    Publishing date 2020-02-13
    Publishing country de
    Document type Article ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Audio / Video ; Online: Dynamics of quinolone- and cephalosporin-resistant Escherichia coli carriage along the production chain in Thuringian pigsties

    Pfeifer, Nicola* / Barth, Stefanie / Higgins, Owen / Chueiri, Alexandra / O’Connor, Louise / Smith, Terry / Berens, Christian / Menge, Christian

    2021  

    Keywords Text ; abstract_or_summary ; ddc:570
    Language English
    Publishing date 2021-06-10
    Publishing country de
    Document type Article ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Portable Differential Detection of CTX-M ESBL Gene Variants,

    Higgins, Owen / Chueiri, Alexandra / O'Connor, Louise / Lahiff, Sinéad / Burke, Liam / Morris, Dearbhaile / Pfeifer, Nicola Maria / Santamarina, Belén González / Berens, Christian / Menge, Christian / Caniça, Manuela / Manageiro, Vera / Kisand, Veljo / Hassan, Marwa M / Gardner, Brian / van Vliet, Arnoud H M / La Ragione, Roberto M / Gonzalez-Zorn, Bruno / Smith, Terry J

    Microbiology spectrum

    2022  Volume 11, Issue 1, Page(s) e0331622

    Abstract: Cefotaximase-Munich (CTX-M) extended-spectrum beta-lactamase (ESBL) enzymes produced ... ...

    Abstract Cefotaximase-Munich (CTX-M) extended-spectrum beta-lactamase (ESBL) enzymes produced by
    MeSH term(s) Humans ; Animals ; Swine ; Escherichia coli ; Escherichia coli Infections/diagnosis ; Escherichia coli Infections/veterinary ; Escherichia coli Infections/epidemiology ; beta-Lactamases/genetics ; Anti-Bacterial Agents ; Enterobacteriaceae/genetics ; DNA
    Chemical Substances beta-lactamase TEM-3 (EC 3.5.2.-) ; beta-lactamase CTX-2 (EC 3.5.2.-) ; beta-Lactamases (EC 3.5.2.6) ; Anti-Bacterial Agents ; DNA (9007-49-2)
    Language English
    Publishing date 2022-12-13
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.03316-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Audio / Video ; Online: Rapid detection and discrimination of closely related Enterobacteriaceae CTX-M group 1 variants, blaCTX-M-1 and blaCTX-M-15, using an internally controlled multiplex loop-primer endonuclease cleavage loop-mediated isothermal amplification (LEC-LAMP) assay

    Higgins, Owen* / Chueiri, Alexandra / O'Connor, Louise / Burke, Liam / Morris, Dearbhaile / Hassan, Marwa M. / van Vliet, Arnoud H. M. / La Ragione, Roberto M. / Pfeifer, Nicola Maria / Santamarina, Belén González / Berens, Christian / Menge, Christian / Smith, Terry

    2021  

    Keywords Text ; abstract_or_summary ; ddc:570
    Language English
    Publishing date 2021-04-28
    Publishing country de
    Document type Article ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Portable Differential Detection of CTX-M ESBL Gene Variants, bla CTX-M-1 and bla CTX-M-15 , from Escherichia coli Isolates and Animal Fecal Samples Using Loop-Primer Endonuclease Cleavage Loop-Mediated Isothermal Amplification

    Higgins, Owen / Chueiri, Alexandra / O'Connor, Louise / Lahiff, Sinéad / Burke, Liam / Morris, Dearbhaile / Pfeifer, Nicola Maria / Santamarina, Belén González / Berens, Christian / Menge, Christian / Caniça, Manuela / Manageiro, Vera / Kisand, Veljo / Hassan, Marwa M. / Gardner, Brian / van Vliet, Arnoud H. M. / La Ragione, Roberto M. / Gonzalez-Zorn, Bruno / Smith, Terry J.

    2022  

    Abstract: Cefotaximase-Munich (CTX-M) extended-spectrum beta-lactamase (ESBL) enzymes produced by Enterobacteriaceae confer resistance to clinically relevant third-generation cephalosporins. CTX-M group 1 variants, CTX-M-1 and CTX-M-15, are the leading ESBL- ... ...

    Abstract Cefotaximase-Munich (CTX-M) extended-spectrum beta-lactamase (ESBL) enzymes produced by Enterobacteriaceae confer resistance to clinically relevant third-generation cephalosporins. CTX-M group 1 variants, CTX-M-1 and CTX-M-15, are the leading ESBL-producing Enterobacteriaceae associated with animal and human infection, respectively, and are an increasing antimicrobial resistance (AMR) global health concern. The blaCTX-M-1 and blaCTX-M-15 genes encoding these variants have an approximate nucleotide sequence similarity of 98.7%, making effective differential diagnostic monitoring difficult. Loop-primer endonuclease cleavage loop-mediated isothermal amplification (LEC-LAMP) enables rapid real-time multiplex pathogen detection with single-base specificity and portable on-site testing. We have developed an internally controlled multiplex CTX-M-1/15 LEC-LAMP assay for the differential detection of blaCTX-M-1 and blaCTX-M-15. Assay analytical specificity was established using a panel of human, animal, and environmental Escherichia coli isolates positive for blaCTX-M-1 (n = 18), blaCTX-M-15 (n = 35), and other closely related blaCTX-Ms (n = 38) from Ireland, Germany, and Portugal, with analytical sensitivity determined using probit regression analysis. Animal fecal sample testing using the CTX-M-1/15 LEC-LAMP assay in combination with a rapid DNA extraction protocol was carried out on porcine fecal samples previously confirmed to be PCR-positive for E. coli blaCTX-M. Portable instrumentation was used to further analyze each fecal sample and demonstrate the on-site testing capabilities of the LEC-LAMP assay with the rapid DNA extraction protocol. The CTX-M-1/15 LEC-LAMP assay demonstrated complete analytical specificity for the differential detection of both variants with sensitive low-level detection of 8.5 and 9.8 copies per reaction for blaCTX-M-1 and blaCTX-M-15, respectively, and E. coli blaCTX-M-1 was identified in all blaCTX-M positive porcine fecal samples tested. IMPORTANCE CTX-M ESBL-producing E. coli is an ...
    Keywords Text ; ddc:570 ; AMR -- CTX-M -- ESBL -- Enterobacteriaceae -- LAMP
    Subject code 630
    Language English
    Publishing date 2022-12-13
    Publisher American Society for Microbiology
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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