Article: Protection of Penaeus monodon against white spot syndrome by continuous oral administration of a low concentration of Bacillus subtilis spores expressing the VP28 antigen
Letters in applied microbiology. 2017 Mar., v. 64, no. 3
2017
Abstract: In this study, Bacillus subtilis spores expressing a chimeric protein, CotBâVP28, were used as a probiotic vaccine to protect black tiger shrimps (Penaeus monodon) against white spot syndrome virus (WSSV) infection. Oral administration of pellets ... ...
| Abstract | In this study, Bacillus subtilis spores expressing a chimeric protein, CotBâVP28, were used as a probiotic vaccine to protect black tiger shrimps (Penaeus monodon) against white spot syndrome virus (WSSV) infection. Oral administration of pellets coated with CotBâVP28 spores (at â¥1 Ã 10â¹Â CFU per g pellet) to shrimps induced immuneârelating phenoloxydase activity (PO) in shrimps after 14 days of feeding (prior challenge) and at day 3 post challenge (1·26 and 1·70 fold increase respectively). A 75% protection rate was obtained by continuous feeding of the sporeâcoated pellets at â¥1 Ã 10â¹Â CFU per g for 14 days prior to WSSV challenge and during all the postchallenge period. Even when the amount of CotBâVP28 spores in feed pellets was reduced down to â¥5 Ã 10â·Â CFU per g and â¥1 Ã 10â¶Â CFU per g, relatively high protection rates of 70 and 67·5%, respectively, were still obtained. By contrast, feeding pellets without spores (untreated group) and with naked spores (PY79 group) at â¥1 Ã 10â¹Â CFU per g could not protect shrimps against WSSV. These data suggest that supplementation of CotBâVP28 spores at low dose of â¥1 Ã 10â¶Â CFU per g could be effective as a prophylactic treatment of WSS for black tiger shrimps. SIGNIFICANCE AND IMPACT OF THE STUDY: This study reports the protective efficacy of Bacillus subtilis CotBâVP28 spores on black tiger shrimps (Penaeus monodon) against white spot syndrome virus infection. Oral administration of pellets coated with CotBâVP28 spores (â¥1 Ã 10â¹Â CFU per g) conferred 75% protection after white spot syndrome virus challenge. Even after reducing CotBâVP28 spores in feed pellets to â¥1 Ã 10â¶Â CFU per g, 67·5% protections was still obtained. These data indicate that supplementation of CotBâVP28 spores at a low dose of â¥1 Ã 10â¶Â CFU per g could be effective in prophylaxis against white spot syndrome in black tiger shrimps. |
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| Keywords | Bacillus subtilis ; Penaeus monodon ; White spot syndrome virus ; antigens ; disease control ; oral administration ; pelleted feeds ; pellets ; probiotics ; recombinant fusion proteins ; shrimp ; spores ; vaccines ; white spot syndrome | |||||
| Language | English | |||||
| Dates of publication | 2017-03 | |||||
| Size | p. 184-191. | |||||
| Publishing place | John Wiley & Sons, Ltd | |||||
| Document type | Article | |||||
| Note | JOURNAL ARTICLE | |||||
| ZDB-ID | 632584-1 | |||||
| ISSN | 1472-765X ; 0266-8254 | |||||
| ISSN (online) | 1472-765X | |||||
| ISSN | 0266-8254 | |||||
| DOI | 10.1111/lam.12708 | |||||
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| Database | NAL-Catalogue (AGRICOLA) |
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