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  1. Article ; Online: Palm Fungi and Their Key Role in Biodiversity Surveys: A Review.

    Pereira, Diana S / Phillips, Alan J L

    Journal of fungi (Basel, Switzerland)

    2023  Volume 9, Issue 11

    Abstract: Over the past three decades, a wealth of studies has shown that palm trees ( ...

    Abstract Over the past three decades, a wealth of studies has shown that palm trees (
    Language English
    Publishing date 2023-11-19
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof9111121
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Phytophthora alticola and Phytophthora cinnamomi on Eucalyptus globulus in Portugal

    Diogo, Eugénio / Machado, Helena / Reis, Ana / Valente, Carlos / Phillips, Alan J.L. / Bragança, Helena

    Eur J Plant Pathol. 2023 Feb., v. 165, no. 2 p.255-269

    2023  

    Abstract: Eucalypt, mostly Eucalyptus globulus, is the exotic tree species occupying the largest area of Portuguese planted forest. Eucalypts were introduced in the country more than one hundred years ago, but it was only in the second half of the twentieth ... ...

    Abstract Eucalypt, mostly Eucalyptus globulus, is the exotic tree species occupying the largest area of Portuguese planted forest. Eucalypts were introduced in the country more than one hundred years ago, but it was only in the second half of the twentieth century that the area of plantations grew with the onset of their use in the paper and pulp industry. As an exotic species, it was free from pests and diseases but gradually the number of insects and pathogens affecting these plants increased. In recent year, a new disease causing root rot and dieback has been detected with increasing frequency. In this work, two Phytophthora species, P. cinnamomi and P. alticola, were identified associated with these symptoms. Koch’s postulates were fulfilled, proving that these two species are pathogenic to E. globulus. This is the first report of P. alticola outside South Africa. Although presently unknown, the possible factors associated with the increased occurrence of this disease are discussed.
    Keywords Eucalyptus globulus ; Phytophthora cinnamomi ; dieback ; forests ; introduced species ; pulp and paper industry ; root rot ; trees ; Portugal ; South Africa
    Language English
    Dates of publication 2023-02
    Size p. 255-269.
    Publishing place Springer Netherlands
    Document type Article ; Online
    ZDB-ID 1196520-4
    ISSN 0929-1873
    ISSN 0929-1873
    DOI 10.1007/s10658-022-02604-9
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Diaporthe

    Pereira, Diana S / Hilário, Sandra / Gonçalves, Micael F M / Phillips, Alan J L

    Microorganisms

    2023  Volume 11, Issue 11

    Abstract: Due to cryptic diversification, phenotypic plasticity and host associations, multilocus phylogenetic analyses have become the most important tool in accurately identifying and circumscribing species in ... ...

    Abstract Due to cryptic diversification, phenotypic plasticity and host associations, multilocus phylogenetic analyses have become the most important tool in accurately identifying and circumscribing species in the
    Language English
    Publishing date 2023-11-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11112717
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Two new

    Sun, Jing-E / Meng, Chao-Rong / Phillips, Alan J L / Wang, Yong

    MycoKeys

    2022  Volume 94, Page(s) 1–16

    Abstract: Five ascomycetous strains were isolated from dead branches and leaves ... ...

    Abstract Five ascomycetous strains were isolated from dead branches and leaves of
    Language English
    Publishing date 2022-11-11
    Publishing country Bulgaria
    Document type Journal Article
    ZDB-ID 2630648-7
    ISSN 1314-4049 ; 1314-4049
    ISSN (online) 1314-4049
    ISSN 1314-4049
    DOI 10.3897/mycokeys.94.91340
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Two new Morinia species from palms (Arecaceae) in Portugal

    Pereira, Diana Santos / Phillips, Alan J. L.

    Mycol Progress. 2021 Feb., v. 20, no. 2 p.83-94

    2021  

    Abstract: During the course of a study of palm fungi in Portugal, several Bartilinia-like taxa were isolated. Morphological characters and phylogenetic data derived from ITS and LSU sequences supported the establishment of two new Morinia species introduced here ... ...

    Abstract During the course of a study of palm fungi in Portugal, several Bartilinia-like taxa were isolated. Morphological characters and phylogenetic data derived from ITS and LSU sequences supported the establishment of two new Morinia species introduced here as M. trachycarpi and M. phoenicicola spp. nov. isolated from foliar diseases of ornamental palm trees in Lisbon, Portugal. The morphological concept of Morinia is broadened to include enclosed pycnidial conidiomata and conidia with wedge-shaped apical cells, new distinctive morphological characteristics observed in M. trachycarpi. Bartalinia robillardoides was also isolated and is presented here as a new host and geographical record on foliar lesions of Phoenix reclinata from Lisbon, Portugal. Comparative morphological data on species of Morinia, as well as a key to species in Morinia, are provided and discussed.
    Keywords Phoenix reclinata ; conidia ; phylogeny ; pycnidia ; taxonomic keys ; Portugal
    Language English
    Dates of publication 2021-02
    Size p. 83-94.
    Publishing place Springer Berlin Heidelberg
    Document type Article ; Online
    ZDB-ID 2226747-5
    ISSN 1861-8952 ; 1617-416X
    ISSN (online) 1861-8952
    ISSN 1617-416X
    DOI 10.1007/s11557-020-01650-z
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Two new Botryosphaeria (Botryosphaeriales, Botryosphaeriaceae) species in China

    Sun, Jing-E / Meng, Chao-Rong / Phillips, Alan J. L. / Wang, Yong

    MycoKeys. 2022 Nov. 11, v. 94 p.1-16

    2022  

    Abstract: AbstractFive ascomycetous strains were isolated from dead branches and leaves of Salix (Salicaceae) and Osmanthus fragrans (Oleaceae), respectively. BLAST searches with ITS sequences in GenBank suggested a high degree of similarity to Botryosphaeria ... ...

    Abstract AbstractFive ascomycetous strains were isolated from dead branches and leaves of Salix (Salicaceae) and Osmanthus fragrans (Oleaceae), respectively. BLAST searches with ITS sequences in GenBank suggested a high degree of similarity to Botryosphaeria dothidea . To accurately identify these strains, we further analysed their morphological characteristics of asci, ascospores, all conidiophore cells and conidia. Phylogenetic relationships, based on ITS, rpb2, tef1 and tub2 gene sequences, confirmed our strains represented two novel species, which are introduced here as B. salicicola and B. osmanthuse spp. nov.
    Keywords Botryosphaeria dothidea ; Osmanthus fragrans ; Salix ; asci ; ascospores ; conidia ; genes ; phylogeny ; China ; Ascomycetes ; molecular analyses ; morphology ; new species ; new woody host
    Language English
    Dates of publication 2022-1111
    Size p. 1-16.
    Publishing place Pensoft Publishers
    Document type Article ; Online
    ZDB-ID 2630648-7
    ISSN 1314-4049
    ISSN 1314-4049
    DOI 10.3897/mycokeys.94.91340
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: Caveats of the internal transcribed spacer region as a barcode to resolve species boundaries in Diaporthe

    Hilário, Sandra / Santos, Liliana / Phillips, Alan J.L. / Alves, Artur

    British Mycological Society Fungal biology. 2022 Jan., v. 126, no. 1

    2022  

    Abstract: Species in Diaporthe are largely reported as important plant pathogens. Identification of species in this genus has been complemented by morphological and molecular features. However, one important factor delaying this process is the struggle to ... ...

    Abstract Species in Diaporthe are largely reported as important plant pathogens. Identification of species in this genus has been complemented by morphological and molecular features. However, one important factor delaying this process is the struggle to formulate robust species concepts to create adequate international phytosanitary measures. Regardless of the wide use of the internal transcribed spacer (ITS) rDNA region, established as the primary DNA barcode for fungi, the tendency for intraspecific variation has been reported, misleading interpretation of phylogenetic analyses. Therefore, the present study aimed to illustrate, using specific examples, how the ITS region may be problematic for species delimitation. We showed that the ITS region is highly variable, with strains of Diaporthe malorum and Diaporthe novem falling into more than one clade, which if analyzed on their own, would be likely recognized as distinct taxa. Divergent ITS paralogs were also proven to coexist within the genome of D. novem. We also suggest that ITS may have escaped from concerted evolution or has undergone a duplication event. Furthermore, this study reports for the first time the existence of a putative hybrid in the genus Diaporthe. Our findings offer new clues towards the intraspecific and intragenomic variation in the ITS region, raising questions about its value for barcoding, i.e., identifying species in the genus Diaporthe. Therefore, we recommend that the ITS region be analyzed cautiously and always compared for congruence prior to description of novel taxa.
    Keywords DNA barcoding ; Diaporthe ; concerted evolution ; genome ; hybrids ; internal transcribed spacers ; intraspecific variation ; mycology ; phylogeny
    Language English
    Dates of publication 2022-01
    Size p. 54-74.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 2532164-X
    ISSN 1878-6162 ; 1878-6146
    ISSN (online) 1878-6162
    ISSN 1878-6146
    DOI 10.1016/j.funbio.2021.10.005
    Database NAL-Catalogue (AGRICOLA)

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  8. Article: Caveats of the internal transcribed spacer region as a barcode to resolve species boundaries in Diaporthe.

    Hilário, Sandra / Santos, Liliana / Phillips, Alan J L / Alves, Artur

    Fungal biology

    2021  Volume 126, Issue 1, Page(s) 54–74

    Abstract: Species in Diaporthe are largely reported as important plant pathogens. Identification of species in this genus has been complemented by morphological and molecular features. However, one important factor delaying this process is the struggle to ... ...

    Abstract Species in Diaporthe are largely reported as important plant pathogens. Identification of species in this genus has been complemented by morphological and molecular features. However, one important factor delaying this process is the struggle to formulate robust species concepts to create adequate international phytosanitary measures. Regardless of the wide use of the internal transcribed spacer (ITS) rDNA region, established as the primary DNA barcode for fungi, the tendency for intraspecific variation has been reported, misleading interpretation of phylogenetic analyses. Therefore, the present study aimed to illustrate, using specific examples, how the ITS region may be problematic for species delimitation. We showed that the ITS region is highly variable, with strains of Diaporthe malorum and Diaporthe novem falling into more than one clade, which if analyzed on their own, would be likely recognized as distinct taxa. Divergent ITS paralogs were also proven to coexist within the genome of D. novem. We also suggest that ITS may have escaped from concerted evolution or has undergone a duplication event. Furthermore, this study reports for the first time the existence of a putative hybrid in the genus Diaporthe. Our findings offer new clues towards the intraspecific and intragenomic variation in the ITS region, raising questions about its value for barcoding, i.e., identifying species in the genus Diaporthe. Therefore, we recommend that the ITS region be analyzed cautiously and always compared for congruence prior to description of novel taxa.
    MeSH term(s) DNA, Ribosomal ; Phylogeny ; Plants ; Saccharomycetales
    Chemical Substances DNA, Ribosomal
    Language English
    Publishing date 2021-10-28
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2532164-X
    ISSN 1878-6162 ; 1878-6146
    ISSN (online) 1878-6162
    ISSN 1878-6146
    DOI 10.1016/j.funbio.2021.10.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Taxonomy and Multigene Phylogeny of

    Wang, Si-Yao / McKenzie, Eric H C / Phillips, Alan J L / Li, Yan / Wang, Yong

    Journal of fungi (Basel, Switzerland)

    2022  Volume 8, Issue 12

    Abstract: In a study of fungi isolated from plant material in Guizhou Province, China, we identified 23 strains ... ...

    Abstract In a study of fungi isolated from plant material in Guizhou Province, China, we identified 23 strains of
    Language English
    Publishing date 2022-12-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof8121301
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Identification and Characterization of Colletotrichum Species Associated with Cherry Leaf Spot Disease in China

    Zhou, Yueyan / Zhang, Wei / Li, Yameng / Ji, Shuxian / Li, Xinghong / Hyde, Kevin D. / Zhang, Kaichun / Phillips, Alan J. L. / Manawasinghe, Ishara S. / Yan, Jiye

    Plant Disease. 2023 Feb. 01, v. 107, no. 2 p.500-513

    2023  

    Abstract: Leaf spot is a common and serious disease of sweet cherry worldwide and has become a major concern in China. From 2018 to 2020, disease investigations were carried out in Beijing City, Sichuan, Shandong, and Liaoning Provinces in China, and 105 ... ...

    Abstract Leaf spot is a common and serious disease of sweet cherry worldwide and has become a major concern in China. From 2018 to 2020, disease investigations were carried out in Beijing City, Sichuan, Shandong, and Liaoning Provinces in China, and 105 Colletotrichum isolates were obtained from diseased samples. Isolates were identified by morphological characterization coupled with multigene phylogenetic analyses based on six loci (internal transcribed spacer region, glyceraldehyde 3-phosphate dehydrogenase, calmodulin, actin, chitin synthase, and β-tubulin). A total of 13 Colletotrichum species were identified, namely Colletotrichum aenigma, C. gloeosporioides, C. fructicola, C. siamense, C. temperatum, C. conoides, C. hebeiense, C. sojae, C. plurivorum, C. karsti, C. truncatum, C. incanum, and C. dematium. Among these, C. aenigma (25.7%) was the most prominent species isolated from diseased leaves, followed by C. gloeosporioides (19.0%) and C. fructicola (12.4%). Pathogenicity was tested on detached leaves of cv. ‘Tieton’ and ‘Summit’ and young seedlings of cv. ‘Brooks’ under greenhouse conditions. All 13 species were pathogenic to cherry leaves, and C. aenigma, C. conoides, and C. dematium showed high levels of virulence. Seedlings inoculated with the isolates developed similar symptoms to those seen in the orchards. This study provides the first reports for 11 of the 13 Colletotrichum species on sweet cherry in the world, excluding C. aenigma and C. fructicola. This is the first comprehensive study of Colletotrichum species associated with cherry leaf spot in China, and the results will provide basic knowledge to develop sustainable control measures for cherry leaf spot.
    Keywords Colletotrichum ; Prunus avium ; actin ; calmodulin ; cherries ; chitin synthase ; glyceraldehyde-3-phosphate dehydrogenase ; greenhouses ; internal transcribed spacers ; leaf spot ; phylogeny ; virulence ; China ; morphological characteristics ; new host record ; pathogenicity ; phylogenetic analysis
    Language English
    Dates of publication 2023-0201
    Size p. 500-513.
    Publishing place The American Phytopathological Society
    Document type Article ; Online
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-11-21-2538-RE
    Database NAL-Catalogue (AGRICOLA)

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