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  1. Article ; Online: Conjugation-Mediated Plasmid Transfer Enables Genetic Modification of Diverse

    Phillips, Elise K / Cannon, Jordan A / Zhou, Yue / Bonifer, Kyle S / Reynolds, Todd B

    Microbiology spectrum

    2023  , Page(s) e0370022

    Abstract: Performing genetic manipulations ... ...

    Abstract Performing genetic manipulations in
    Language English
    Publishing date 2023-03-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.03700-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Genome sequences of four agarolytic bacteria from the Bacteroidia and Gammaproteobacteria.

    Phillips, Elise K / Shaffer, Jacob M C / Henson, Michael W / Coelho, Jordan T / Martin, Mark O / Thrash, J Cameron

    Microbiology resource announcements

    2023  Volume 12, Issue 11, Page(s) e0066723

    Abstract: Here we present the genomes of four marine agarolytic bacteria belonging to the Bacteroidota and Proteobacteria. Two genomes are closed and two are in draft form, but all are at least 99% complete and offer new opportunities to study agar-degradation in ... ...

    Abstract Here we present the genomes of four marine agarolytic bacteria belonging to the Bacteroidota and Proteobacteria. Two genomes are closed and two are in draft form, but all are at least 99% complete and offer new opportunities to study agar-degradation in marine bacteria.
    Language English
    Publishing date 2023-10-09
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/MRA.00667-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: The small molecule CBR-5884 inhibits the

    Zhou, Yue / Phelps, Gregory A / Mangrum, Mikayla M / McLeish, Jemma / Phillips, Elise K / Lou, Jinchao / Ancajas, Christelle F / Rybak, Jeffrey M / Oelkers, Peter M / Lee, Richard E / Best, Michael D / Reynolds, Todd B

    mBio

    2024  Volume 15, Issue 5, Page(s) e0063324

    Abstract: Systemic infections by : Importance: Fungal phosphatidylserine synthase (Cho1) is a logical antifungal target due to its crucial role in the virulence and viability of various fungal pathogens, and since it is absent in humans, drugs targeted at Cho1 ... ...

    Abstract Systemic infections by
    Importance: Fungal phosphatidylserine synthase (Cho1) is a logical antifungal target due to its crucial role in the virulence and viability of various fungal pathogens, and since it is absent in humans, drugs targeted at Cho1 are less likely to cause toxicity in patients. Using fungal Cho1 as a model, there have been two unsuccessful attempts to discover inhibitors for Cho1 homologs in whole-cell screens prior to this study. The compounds identified in these attempts do not act directly on the protein, resulting in the absence of known Cho1 inhibitors. The significance of our research is that we developed a high-throughput target-based assay and identified the first Cho1 inhibitor, CBR-5884, which acts both on the purified protein and its function in the cell. This molecule acts as a competitive inhibitor with a
    MeSH term(s) Candida albicans/drug effects ; Candida albicans/enzymology ; Candida albicans/genetics ; Antifungal Agents/pharmacology ; Antifungal Agents/chemistry ; CDPdiacylglycerol-Serine O-Phosphatidyltransferase/genetics ; CDPdiacylglycerol-Serine O-Phosphatidyltransferase/metabolism ; CDPdiacylglycerol-Serine O-Phosphatidyltransferase/chemistry ; Enzyme Inhibitors/pharmacology ; Enzyme Inhibitors/chemistry ; High-Throughput Screening Assays ; Small Molecule Libraries/pharmacology ; Small Molecule Libraries/chemistry ; Microbial Sensitivity Tests ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Fungal Proteins/antagonists & inhibitors ; Fungal Proteins/chemistry ; Phosphatidylserines/metabolism ; Furans ; Thiophenes
    Chemical Substances Antifungal Agents ; CDPdiacylglycerol-Serine O-Phosphatidyltransferase (EC 2.7.8.8) ; Enzyme Inhibitors ; Small Molecule Libraries ; Fungal Proteins ; Phosphatidylserines ; cbr-5884 ; Furans ; Thiophenes
    Language English
    Publishing date 2024-04-09
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2557172-2
    ISSN 2150-7511 ; 2161-2129
    ISSN (online) 2150-7511
    ISSN 2161-2129
    DOI 10.1128/mbio.00633-24
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Mucosal Infection with Unmasked Candida albicans Cells Impacts Disease Progression in a Host Niche-Specific Manner.

    Wagner, Andrew S / Vogel, Amanda K / Lumsdaine, Stephen W / Phillips, Elise K / Willems, Hubertine M E / Peters, Brian M / Reynolds, Todd B

    Infection and immunity

    2022  Volume 90, Issue 12, Page(s) e0034222

    Abstract: Shielding the immunogenic cell wall epitope β(1, 3)-glucan under an outer layer of mannosylated glycoproteins is an essential virulence factor deployed by Candida albicans during systemic infection. Accordingly, mutants with increased β(1, 3)-glucan ... ...

    Abstract Shielding the immunogenic cell wall epitope β(1, 3)-glucan under an outer layer of mannosylated glycoproteins is an essential virulence factor deployed by Candida albicans during systemic infection. Accordingly, mutants with increased β(1, 3)-glucan exposure (unmasking) display increased immunostimulatory capabilities
    MeSH term(s) Animals ; Female ; Male ; Mice ; Candida albicans ; Candidiasis/microbiology ; Candidiasis, Oral ; Candidiasis, Vulvovaginal/microbiology ; Disease Progression ; Glucans
    Chemical Substances Glucans
    Language English
    Publishing date 2022-11-14
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    DOI 10.1128/iai.00342-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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    Jg. 1995 - 2021: Lesesall (1.OG)
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  5. Article ; Online: Activation of Cph1 causes ß(1,3)-glucan unmasking in Candida albicans and attenuates virulence in mice in a neutrophil-dependent manner.

    Wagner, Andrew S / Hancock, Trevor J / Lumsdaine, Stephen W / Kauffman, Sarah J / Mangrum, Mikayla M / Phillips, Elise K / Sparer, Timothy E / Reynolds, Todd B

    PLoS pathogens

    2021  Volume 17, Issue 8, Page(s) e1009839

    Abstract: Masking the immunogenic cell wall epitope ß(1,3)-glucan under an outer layer of mannosylated glycoproteins is an important virulence factor deployed by Candida albicans during infection. Consequently, increased ß(1,3)-glucan exposure (unmasking) reveals ... ...

    Abstract Masking the immunogenic cell wall epitope ß(1,3)-glucan under an outer layer of mannosylated glycoproteins is an important virulence factor deployed by Candida albicans during infection. Consequently, increased ß(1,3)-glucan exposure (unmasking) reveals C. albicans to the host's immune system and attenuates its virulence. We have previously shown that activation of the Cek1 MAPK pathway via expression of a hyperactive allele of an upstream kinase (STE11ΔN467) induced unmasking. It also increased survival of mice in a murine disseminated candidiasis model and attenuated kidney fungal burden by ≥33 fold. In this communication, we utilized cyclophosphamide-induced immunosuppression to test if the clearance of the unmasked STE11ΔN467 mutant was dependent on the host immune system. Suppression of the immune response by cyclophosphamide reduced the attenuation in fungal burden caused by the STE11ΔN467 allele. Moreover, specific depletion of neutrophils via 1A8 antibody treatment also reduced STE11ΔN467-dependent fungal burden attenuation, but to a lesser extent than cyclophosphamide, demonstrating an important role for neutrophils in mediating fungal clearance of unmasked STE11ΔN467 cells. In an effort to understand the mechanism by which Ste11ΔN467 causes unmasking, transcriptomics were used to reveal that several components in the Cek1 MAPK pathway were upregulated, including the transcription factor CPH1 and the cell wall sensor DFI1. In this report we show that a cph1ΔΔ mutation restored ß(1,3)-glucan exposure to wild-type levels in the STE11ΔN467 strain, confirming that Cph1 is the transcription factor mediating Ste11ΔN467-induced unmasking. Furthermore, Cph1 is shown to induce a positive feedback loop that increases Cek1 activation. In addition, full unmasking by STE11ΔN467 is dependent on the upstream cell wall sensor DFI1. However, while deletion of DFI1 significantly reduced Ste11ΔN467-induced unmasking, it did not impact activation of the downstream kinase Cek1. Thus, it appears that once stimulated by Ste11ΔN467, Dfi1 activates a parallel signaling pathway that is involved in Ste11ΔN467-induced unmasking.
    MeSH term(s) Animals ; Candida albicans/immunology ; Candidiasis/immunology ; Candidiasis/microbiology ; Candidiasis/prevention & control ; Cell Wall ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Gene Expression Regulation, Fungal ; Mice ; Mice, Inbred ICR ; Neutrophils/immunology ; Neutrophils/microbiology ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Virulence ; beta-Glucans/immunology
    Chemical Substances CPH1 protein, Candida albicans ; Fungal Proteins ; Transcription Factors ; beta-Glucans
    Language English
    Publishing date 2021-08-25
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7374
    ISSN (online) 1553-7374
    ISSN 1553-7374
    DOI 10.1371/journal.ppat.1009839
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Bacillus pumilus

    Bonifer, Kyle S / Wen, Xianfang / Hasim, Sahar / Phillips, Elise K / Dunlap, Rachel N / Gann, Eric R / DeBruyn, Jennifer M / Reynolds, Todd B

    Frontiers in microbiology

    2019  Volume 10, Page(s) 2548

    Abstract: Poly-lactic acid (PLA) is increasingly used as a biodegradable alternative to traditional petroleum-based plastics. In this study, we identify a novel agricultural soil isolate ... ...

    Abstract Poly-lactic acid (PLA) is increasingly used as a biodegradable alternative to traditional petroleum-based plastics. In this study, we identify a novel agricultural soil isolate of
    Language English
    Publishing date 2019-11-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2019.02548
    Database MEDical Literature Analysis and Retrieval System OnLINE

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