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Article ; Online: Performance of a flow cytometry-based immunoassay for detection of antibodies binding to SARS-CoV-2 spike protein.

Valdivia, Arantxa / Tarín, Fabián / Alcaraz, María Jesús / Piñero, Paula / Torres, Ignacio / Marco, Francisco / Albert, Eliseo / Navarro, David

Scientific reports

2022 Volume 12, Issue 1, Page(s) 586

Abstract: The performance of a laboratory-developed IgG/IgA flow cytometry-based immunoassay (FCI) using Jurkat T cells stably expressing full-length native S protein was compared against Elecsys electrochemiluminiscent (ECLIA) Anti-SARS-CoV-2 S (Roche Diagnostics, ...

Abstract	The performance of a laboratory-developed IgG/IgA flow cytometry-based immunoassay (FCI) using Jurkat T cells stably expressing full-length native S protein was compared against Elecsys electrochemiluminiscent (ECLIA) Anti-SARS-CoV-2 S (Roche Diagnostics, Pleasanton, CA, USA), and Liaison SARS-CoV-2 TrimericS IgG chemiluminiscent assay (CLIA) (Diasorin S.p.a, Saluggia, IT) for detection of SARS-CoV-2-specific antibodies. A total of 225 serum/plasma specimens from 120 acute or convalescent COVID-19 individuals were included. Overall, IgG/IgA-FCI yielded the highest number of positives (n = 179), followed by IgA-FCI (n = 177), Roche ECLIA (n = 175), IgG-FCI (n = 172) and Diasorin CLIA (n = 154). For sera collected early after the onset of symptoms (within 15 days) IgG/IgA-FCI also returned the highest number of positive results (52/72; 72.2%). Positive percent agreement between FCI and compared immunoassays was highest for Roche ECLIA, ranging from 96.1 (IgG/IgA-FCI) to 97.7% (IgG-FCI), whereas negative percent agreement was higher between FCI and Diasosin CLIA, regardless of antibody isotype. The data suggest that FCI may outperform Roche ECLIA and Diasorin CLIA in terms of clinical sensitivity for serological diagnosis of SARS-CoV-2 infection.
MeSH term(s)	Antibodies, Viral/blood ; Antibodies, Viral/immunology ; COVID-19 Serological Testing ; Flow Cytometry/methods ; Humans ; Immunoassay/methods ; Jurkat Cells ; Retrospective Studies ; SARS-CoV-2/immunology ; Sensitivity and Specificity ; Spike Glycoprotein, Coronavirus/immunology
Chemical Substances	Antibodies, Viral ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
Language	English
Publishing date	2022-01-12
Publishing country	England
Document type	Evaluation Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-04565-1
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Article ; Online: Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection.

Piñero, Paula / Marco De La Calle, Francisco M / Horndler, Lydia / Alarcón, Balbino / Uribe Barrientos, Marisol / Sarmiento, Héctor / Tarín, Fabián

Scientific reports

2021 Volume 11, Issue 1, Page(s) 10716

Abstract: SARS-CoV-2 is the virus that causes the disease called COVID-19, which has caused the worst pandemic of the century. Both, to know the immunological status of general population and to evaluate the efficacy of the vaccination process that is taking place ...

Abstract	SARS-CoV-2 is the virus that causes the disease called COVID-19, which has caused the worst pandemic of the century. Both, to know the immunological status of general population and to evaluate the efficacy of the vaccination process that is taking place around the world, serological tests represent a key tool. Classic serological tests, based on colorimetric techniques, such as ELISA or CLIA, continue to be the most widely used option. However, a real improvement in results is still needed. We developed a highly sensitive and specific FCM assay that allows the detection of IgG and IgA antibodies, directed against the native and functional S-protein of SARS-CoV-2 exposed on the membrane of a transfected cell line, up to 8 months after infection.
MeSH term(s)	Adult ; Aged ; Antibodies, Viral/immunology ; COVID-19/immunology ; Female ; Flow Cytometry ; Humans ; Immunoglobulin A/immunology ; Immunoglobulin G/immunology ; Jurkat Cells ; Male ; Middle Aged ; SARS-CoV-2/immunology ; Spike Glycoprotein, Coronavirus/immunology
Chemical Substances	Antibodies, Viral ; Immunoglobulin A ; Immunoglobulin G ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
Language	English
Publishing date	2021-05-21
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-90054-4
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Article: Identification of Leukemia-Associated Immunophenotypes by Databaseguided Flow Cytometry Provides a Highly Sensitive and Reproducible Strategy for the Study of Measurable Residual Disease in Acute Myeloblastic Leukemia.

Piñero, Paula / Morillas, Marina / Gutierrez, Natalia / Barragán, Eva / Such, Esperanza / Breña, Joaquin / García-Hernández, María C / Gil, Cristina / Botella, Carmen / González-Navajas, José M / Zapater, Pedro / Montesinos, Pau / Sempere, Amparo / Tarín, Fabian

Cancers

2022 Volume 14, Issue 16

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2022-08-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527080-1
ISSN	2072-6694
ISSN	2072-6694
DOI	10.3390/cancers14164010
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Article ; Online: Performance Comparison of a Flow Cytometry-based and Two Commercial Chemiluminescent Immunoassays for Detection and Quantification of Antibodies Binding to SARS-CoV-2 Spike Protein

Valdivia, Arantxa / Tarín, Fabián / Alcaraz, María Jesús / Piñero, Paula / Torres, Ignacio / Marco, Francisco / Albert, Eliseo / Navarro, David

medRxiv

Abstract: The performance of a laboratory-developed quantitative IgG/IgA flow cytometry-based immunoassay (FCI) using Jurkat T cells stably expressing full-length native S protein was compared against Elecsys® electrochemiluminiscent (ECLIA) Anti-SARS-CoV-2 S ( ... ...

Abstract	The performance of a laboratory-developed quantitative IgG/IgA flow cytometry-based immunoassay (FCI) using Jurkat T cells stably expressing full-length native S protein was compared against Elecsys® electrochemiluminiscent (ECLIA) Anti-SARS-CoV-2 S (Roche Diagnostics, Pleasanton, CA, USA), and LIAISON® SARS-CoV-2 TrimericS IgG chemiluminiscent assay (CLIA) (Diasorin S.p.a, Saluggia, IT) for detection and quantitation of SARS-CoV-2-specific antibodies. A total of 225 serum/plasma specimens from 120 acute or convalescent COVID-19 individuals were included. Overall, IgG/IgA-FCI yielded the highest number of positives (n=179), followed by IgA-FCI (n=177), Roche ECLIA (n=175), IgG-FCI (n=172) and Diasorin CLIA (n=154). Positive percent agreement between FCI and compared immunoassays was highest for Roche ECLIA, ranging from 96.1% (IgG/IgA-FCI) to 97.7% (IgG-FCI), whereas negative percent agreement was higher between FCI and Diasosin CLIA, regardless of antibody isotype. A strong correlation (Rho:0.6-0.8) was found between IgG-FCI or IgA-FCI levels and antibodies quantified by Roche ECLIA and Diasorin CLIA. The trajectory of antibody levels delineated by the different immunoassays in 22 of patients with sequential specimens (>=3) was frequently discordant, with the exception of IgG and IgA determined by FCI assay and to a lesser extent antibodies quantified by Roche ECLIA and Diasorin CLIA. The data suggest that FCI may outperform Roche ECLIA and Diasorin CLIA in terms of clinical sensitivity for serological diagnosis of SARS-CoV-2 infection.
Keywords	covid19
Language	English
Publishing date	2021-04-09
Publisher	Cold Spring Harbor Laboratory Press
Document type	Article ; Online
DOI	10.1101/2021.04.06.21254995
Database	COVID19

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Article ; Online: Liver Sinusoidal Endothelial Cells Contribute to Hepatic Antigen-Presenting Cell Function and Th17 Expansion in Cirrhosis.

Caparrós, Esther / Juanola, Oriol / Gómez-Hurtado, Isabel / Puig-Kroger, Amaya / Piñero, Paula / Zapater, Pedro / Linares, Raquel / Tarín, Fabián / Martínez-López, Sebastián / Gracia-Sancho, Jordi / González-Navajas, José M / Francés, Rubén

Cells

2020 Volume 9, Issue 5

Abstract: Hepatic immune function is compromised during cirrhosis. This study investigated the immune features of liver sinusoidal endothelial cells (LSECs) in two experimental models of cirrhosis. Dendritic cells, hepatic macrophages, and LSECs were isolated from ...

Abstract	Hepatic immune function is compromised during cirrhosis. This study investigated the immune features of liver sinusoidal endothelial cells (LSECs) in two experimental models of cirrhosis. Dendritic cells, hepatic macrophages, and LSECs were isolated from carbon tetrachloride and bile duct-ligated rats. Gene expression of innate receptors, bacterial internalization, co-stimulatory molecules induction, and CD4+ T cell activation and differentiation were evaluated. Induced bacterial peritonitis and norfloxacin protocols on cirrhotic rats were also carried out. LSECs demonstrated an active immunosurveillance profile, as shown by transcriptional modulation of different scavenger and cell-adhesion genes, and their contribution to bacterial internalization. LSECs significantly increased their expression of CD40 and CD80 and stimulated CD4+ T cell activation marker CD71 in both models. The pro-inflammatory Th17 subset was expanded in CCl
MeSH term(s)	Adaptive Immunity/drug effects ; Animals ; Antigen-Presenting Cells/drug effects ; Antigen-Presenting Cells/pathology ; Biomarkers/metabolism ; Cell Differentiation/drug effects ; Cell Membrane/drug effects ; Cell Membrane/metabolism ; Cell Proliferation/drug effects ; Endocytosis/drug effects ; Endothelial Cells/drug effects ; Endothelial Cells/metabolism ; Endothelial Cells/pathology ; Escherichia coli/metabolism ; Immunity, Innate/drug effects ; Liver/pathology ; Liver Cirrhosis/immunology ; Liver Cirrhosis/pathology ; Lymphocyte Activation/drug effects ; Male ; Microbial Viability/drug effects ; Monitoring, Immunologic ; Norfloxacin/pharmacology ; Rats, Sprague-Dawley ; Receptors, Immunologic/metabolism ; T-Lymphocytes, Regulatory/drug effects ; T-Lymphocytes, Regulatory/immunology ; Th17 Cells/immunology ; Transcription, Genetic/drug effects
Chemical Substances	Biomarkers ; Receptors, Immunologic ; Norfloxacin (N0F8P22L1P)
Language	English
Publishing date	2020-05-15
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2661518-6
ISSN	2073-4409 ; 2073-4409
ISSN (online)	2073-4409
ISSN	2073-4409
DOI	10.3390/cells9051227
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Article ; Online: Toll-like receptor polymorphisms compromise the inflammatory response against bacterial antigen translocation in cirrhosis.

Piñero, Paula / Juanola, Oriol / Caparrós, Esther / Zapater, Pedro / Giménez, Paula / González-Navajas, José M / Such, José / Francés, Rubén

Scientific reports

2017 Volume 7, Page(s) 46425

Abstract: Bacterial translocation is associated with clinically relevant complications in cirrhosis. We evaluated the effect of toll-like receptor polymorphisms in the soluble response against these episodes. Consecutive patients with cirrhosis and ascitic fluid ... ...

Abstract	Bacterial translocation is associated with clinically relevant complications in cirrhosis. We evaluated the effect of toll-like receptor polymorphisms in the soluble response against these episodes. Consecutive patients with cirrhosis and ascitic fluid were distributed by TLR2 rs4696480, TLR4 rs4986790, and TLR9 rs187084 single-nucleotide polymorphisms. Lipoteichoic acid, lipopolyssaccharide, bacterial-DNA, pro-inflammatory cytokines and nitric oxide levels were quantified in serum samples. In vitro response against specific ligands in variant TLR genotypes was evaluated. One hundred and fourteen patients were included. Variant TLR-2, TLR-4 and TLR-9 SNP genotypes were associated with significantly increased serum levels of LTA, LPS and bacterial-DNA. TNF-α, IL-6 and nitric oxide serum levels were significantly decreased in all variant TLR genotyped patients. Cytokine levels were significantly less upregulated in response to specific TLR-ligands in patients with all variant vs wildtype TLR genotypes. Although in vitro gene expression levels of all wildtype and variant TLRs were similar, MyD88 and NFkB were significantly downregulated in cells from TLR-variant genotyped patients in response to their ligands. Variant TLR genotypes are associated with an increased circulating antigen burden and a decreased proinflammatory response in cirrhosis. This immunodeficiency may facilitate bacteria-related complications in cirrhosis and enhance TLR targeting for its management.
MeSH term(s)	Aged ; Bacterial Infections/genetics ; Bacterial Infections/immunology ; Cytokines/blood ; DNA, Bacterial/immunology ; Female ; Humans ; Lipopolysaccharides/blood ; Liver Cirrhosis/genetics ; Liver Cirrhosis/immunology ; Liver Cirrhosis/microbiology ; Male ; Middle Aged ; Nitric Oxide/blood ; Polymorphism, Single Nucleotide ; Prospective Studies ; Teichoic Acids/blood ; Toll-Like Receptor 2/genetics ; Toll-Like Receptor 4/genetics ; Toll-Like Receptor 9/genetics
Chemical Substances	Cytokines ; DNA, Bacterial ; Lipopolysaccharides ; TLR2 protein, human ; TLR4 protein, human ; TLR9 protein, human ; Teichoic Acids ; Toll-Like Receptor 2 ; Toll-Like Receptor 4 ; Toll-Like Receptor 9 ; Nitric Oxide (31C4KY9ESH) ; lipoteichoic acid (56411-57-5)
Language	English
Publishing date	2017-04-18
Publishing country	England
Document type	Journal Article ; Observational Study ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/srep46425
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Article ; Online: Regulatory T Cells Restrict Permeability to Bacterial Antigen Translocation and Preserve Short-Chain Fatty Acids in Experimental Cirrhosis.

Juanola, Oriol / Piñero, Paula / Gómez-Hurtado, Isabel / Caparrós, Esther / García-Villalba, Rocío / Marín, Alicia / Zapater, Pedro / Tarín, Fabián / González-Navajas, José M / Tomás-Barberán, Francisco A / Francés, Rubén

Hepatology communications

2018 Volume 2, Issue 12, Page(s) 1610–1623

Abstract: Intestinal permeability to translocation of bacterial products is increased in cirrhosis. Regulatory T cells (Tregs) remain central to the interplay between the host and microbial milieu. We propose that Tregs are involved in promoting gut barrier ... ...

Abstract	Intestinal permeability to translocation of bacterial products is increased in cirrhosis. Regulatory T cells (Tregs) remain central to the interplay between the host and microbial milieu. We propose that Tregs are involved in promoting gut barrier integrity and a balanced interaction with gut microbiota-derived short-chain fatty acids (SCFAs). Carbon tetrachloride cirrhosis was induced in wild-type and recombination activating gene 1 (
Language	English
Publishing date	2018-10-22
Publishing country	United States
Document type	Journal Article
ISSN	2471-254X
ISSN (online)	2471-254X
DOI	10.1002/hep4.1268
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Article ; Online: IL26 modulates cytokine response and anti-TNF consumption in Crohn's disease patients with bacterial DNA.

Piñero, Paula / Juanola, Oriol / Gutiérrez, Ana / Zapater, Pedro / Giménez, Paula / Steinert, Anna / Sempere, Laura / González-Navajas, José M / Niess, Jan H / Francés, Rubén

Journal of molecular medicine (Berlin, Germany)

2017 Volume 95, Issue 11, Page(s) 1227–1236

Abstract: Interleukin IL26 supports killing of microbes and the innate sensing of bacterial-derived DNA (bactDNA). We evaluated the relationship between IL26 serum levels and bactDNA translocation in Crohn's disease (CD). We ran a prospective study on CD patients ... ...

Abstract	Interleukin IL26 supports killing of microbes and the innate sensing of bacterial-derived DNA (bactDNA). We evaluated the relationship between IL26 serum levels and bactDNA translocation in Crohn's disease (CD). We ran a prospective study on CD patients in remission. IL26 common polymorphisms, serum cytokines and complement protein, amplified-bactDNA, and anti-TNF-α were evaluated. In vitro PBMC analysis was performed. Three hundred and thirteen patients were included (mean CDAI: 83.6 ± 32.8; mean fecal calprotectin: 55.4 ± 35.3 μg/g). A total of 106 patients (33.8%) showed bactDNA and 223 patients (71%) had a varIL26 genotype. BactDNA significantly correlated with increased IL26 levels compared with bactDNA-negative patients. PBMCs from varIL26 patients significantly reduced E. coli killing capacity compared with wtIL26-genotyped patients. The stimulation with a recombinant IL26 protein reduced pro-inflammatory cytokines in response to E. coli in the varIL26 cell supernatants. Serum anti-TNF-α levels in varIL26 vs wtIL26-genotyped patients on biologics were significantly lower in the presence of bactDNA. Cells from varIL26 vs wtIL26-genotyped patients cultured with E. coli DNA and infliximab showed a significant decrease in free anti-TNF-α concentration. A varIL26 genotype was associated with the initiation of anti-TNF-α in CD patients during the 6-month follow-up. IL26 polymorphisms may prevent bactDNA clearance and identify CD patients with a worse inflammatory evolution and response to therapy. Key messages: BactDNA translocation in CD is associated with an increased risk of relapse. IL26 is sensitive to bactDNA and modulates the inflammatory response in CD patients. The varIL26 genotype is associated with reduced PMN capacity to kill bacteria. A varIL26 genotype is associated with decreased levels of anti-TNF-α in CD patients. IL26 may help explain the role of bactDNA as a risk factor of flare in CD patients.
MeSH term(s)	Adult ; Anti-Inflammatory Agents/therapeutic use ; Antibodies, Monoclonal/therapeutic use ; Crohn Disease/diagnosis ; Crohn Disease/drug therapy ; Crohn Disease/genetics ; Crohn Disease/immunology ; Cytokines/metabolism ; DNA, Bacterial/immunology ; Female ; Genotype ; Humans ; Interleukins/blood ; Interleukins/genetics ; Leukocytes/immunology ; Leukocytes/metabolism ; Male ; Middle Aged ; Polymorphism, Single Nucleotide ; Tumor Necrosis Factor-alpha/antagonists & inhibitors
Chemical Substances	Anti-Inflammatory Agents ; Antibodies, Monoclonal ; Cytokines ; DNA, Bacterial ; IL26 protein, human ; Interleukins ; Tumor Necrosis Factor-alpha
Language	English
Publishing date	2017-09-06
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1223802-8
ISSN	1432-1440 ; 0946-2716
ISSN (online)	1432-1440
ISSN	0946-2716
DOI	10.1007/s00109-017-1585-6
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Article ; Online: Selective intestinal decontamination with norfloxacin enhances a regulatory T cell-mediated inflammatory control mechanism in cirrhosis.

Juanola, Oriol / Gómez-Hurtado, Isabel / Zapater, Pedro / Moratalla, Alba / Caparrós, Esther / Piñero, Paula / González-Navajas, José M / Giménez, Paula / Such, José / Francés, Rubén

Liver international : official journal of the International Association for the Study of the Liver

2016 Volume 36, Issue 12, Page(s) 1811–1820

Abstract: Background & aims: Norfloxacin exerts immunomodulatory effects in cirrhosis beyond its bactericidal activity. We aimed at identifying the role of regulatory T (Treg) cells in the norfloxacin mechanism that compensates the inflammatory environment in ... ...

Abstract	Background & aims: Norfloxacin exerts immunomodulatory effects in cirrhosis beyond its bactericidal activity. We aimed at identifying the role of regulatory T (Treg) cells in the norfloxacin mechanism that compensates the inflammatory environment in cirrhosis. Patients & methods: Consecutively admitted patients with cirrhosis and ascitic fluid (AF) with: spontaneous bacterial peritonitis (SBP), non-infected AF, and norfloxacin as secondary SBP prophylaxis (SID group). Tregs were defined by flow-cytometry as CD4 Results: Eighty-four patients were included. Treg percentage was significantly increased in SID patients compared with SBP or non-infected AF patients. A positive correlation was observed between Tregs and serum norfloxacin and IL-10 levels. DCs from SID patients showed a significantly decreased expression of CD80 and CD86 compared with SBP and non-infected AF patients and correlated with norfloxacin levels. Modulation of co-stimulatory signalling by norfloxacin was not detected in Rag1-deficient mice and Rag1-deficient mice reconstituted with naïve T-cells. However, reconstitution with naïve T-cells and Tregs was associated with significantly downregulated CD80 and CD86 expression in the presence of norfloxacin. Norfloxacin immunomodulatory effect on IL-2 and IFN-gamma reduction and on the increase of IL-10 was significantly achieved only when the Tregs were restored in Rag1-deficient mice. Conclusions: These results provide a plausible mechanism for the immunomodulatory effects of norfloxacin in cirrhosis beyond its bactericidal effect.
MeSH term(s)	Adoptive Transfer ; Aged ; Animals ; Anti-Bacterial Agents/therapeutic use ; B7-1 Antigen/metabolism ; B7-2 Antigen/metabolism ; Bacterial Infections/drug therapy ; Bacterial Translocation/drug effects ; Dendritic Cells/drug effects ; Female ; Forkhead Transcription Factors/metabolism ; Humans ; Interleukin-10/blood ; Interleukin-2/blood ; Liver Cirrhosis/complications ; Liver Cirrhosis/microbiology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Middle Aged ; Norfloxacin/therapeutic use ; Peritonitis/drug therapy ; Peritonitis/microbiology ; T-Lymphocytes, Regulatory/drug effects ; T-Lymphocytes, Regulatory/immunology
Chemical Substances	Anti-Bacterial Agents ; B7-1 Antigen ; B7-2 Antigen ; Forkhead Transcription Factors ; IL10 protein, human ; IL2 protein, human ; Interleukin-2 ; Interleukin-10 (130068-27-8) ; Norfloxacin (N0F8P22L1P)
Language	English
Publishing date	2016-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	2102783-3
ISSN	1478-3231 ; 1478-3223
ISSN (online)	1478-3231
ISSN	1478-3223
DOI	10.1111/liv.13172
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Article ; Online: Bifidobacterium pseudocatenulatum CECT7765 induces an M2 anti-inflammatory transition in macrophages from patients with cirrhosis.

Moratalla, Alba / Caparrós, Esther / Juanola, Oriol / Portune, Kevin / Puig-Kröger, Amaya / Estrada-Capetillo, Lizbeth / Bellot, Pablo / Gómez-Hurtado, Isabel / Piñero, Paula / Zapater, Pedro / González-Navajas, José M / Such, José / Sanz, Yolanda / Francés, Rubén

Journal of hepatology

2016 Volume 64, Issue 1, Page(s) 135–145

Abstract: Background & aims: Patients with cirrhosis show recurrent access of bacterial products into the bloodstream inducing a multi-altered immunological status leading to relevant complications. We aimed at evaluating Bifidobacterium pseudocatenulatum ... ...

Abstract	Background & aims: Patients with cirrhosis show recurrent access of bacterial products into the bloodstream inducing a multi-altered immunological status leading to relevant complications. We aimed at evaluating Bifidobacterium pseudocatenulatum CECT7765 effect on the host's macrophage function. Patients & methods: Patients with cirrhosis and ascites were included. Granulocyte-macrophage colony-stimulating factor (GM-CSF) monocyte-derived and ascitic fluid (AF) macrophages were cultured with M-CSF, lipopolysaccharide (LPS) and/or the bifidobacterial strain. Pellets and supernatants were evaluated for gene expression of M1 and M2-related genes and cytokine secretion. Cell surface expression molecules were evaluated by flow cytometry. Kupffer cells from bile duct ligated and CCl4 rats were also evaluated. Results: Experiments were run on GM-CSF blood-derived and AF macrophages from 10 patients with cirrhosis and 10 healthy donors. Different macrophage morphology was observed by optical microscopy in cells stimulated with bifidobacteria vs. LPS. M2-like expression of CD206, CD163 and CD16 was significantly increased in macrophages after stimulation with the bifidobacterial strain vs. LPS. B. pseudocatenulatum CECT7765 was able to significantly change the cytokine secretion pattern of blood-derived and AF macrophages and Kupffer cells from bile duct ligated and CCl4 cirrhotic rats compared to that induced by LPS. B. pseudocatenulatum CECT7765 was also effective in inducing a phenotype transition and a functional change from an M1- to an M2-related gene expression and cytokine secretion pattern in AF macrophages even after LPS-pretreatment. B. pseudocatenulatum CECT7765 did not reduce AF macrophage bacterial killing capacity. Conclusion: B. pseudocatenulatum CECT7765 induces a morphologic, phenotypic and functional transition towards an anti-inflammatory profile in GM-CSF monocyte-derived and AF macrophages from patients with cirrhosis that may help in controlling sustained inflammation in decompensated cirrhosis.
MeSH term(s)	Aged ; Anti-Inflammatory Agents/pharmacology ; Bifidobacterium ; Cell Polarity ; Cytokines/biosynthesis ; Female ; Humans ; Kupffer Cells/immunology ; Lipopolysaccharides/pharmacology ; Liver Cirrhosis/immunology ; Macrophages/immunology ; Male ; Middle Aged ; Prospective Studies
Chemical Substances	Anti-Inflammatory Agents ; Cytokines ; Lipopolysaccharides
Language	English
Publishing date	2016-01
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605953-3
ISSN	1600-0641 ; 0168-8278
ISSN (online)	1600-0641
ISSN	0168-8278
DOI	10.1016/j.jhep.2015.08.020
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