LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 52

Search options

  1. Article: The Endangered giant nuthatch Sitta magna: population size, habitat availability and the implications for its conservation in Thailand

    Khamcha, Daphawan / Angkaew, Rongrong / Pierce, Andrew J. / Gale, George A.

    Oryx. 2022 Mar., v. 56, no. 2

    2022  

    Abstract: Available habitat and hence the global population of the Endangered giant nuthatch Sitta magna, restricted to lower montane habitats of south-western China, eastern Myanmar and northern Thailand, remains poorly quantified. Thailand is the only portion of ...

    Abstract Available habitat and hence the global population of the Endangered giant nuthatch Sitta magna, restricted to lower montane habitats of south-western China, eastern Myanmar and northern Thailand, remains poorly quantified. Thailand is the only portion of the species’ range for which there is a population estimate. To obtain a more precise estimate of the Thai population and clarify the extent and characteristics of suitable habitat remaining, we conducted 335 point-count surveys at 67 points across eight localities during November 2019–February 2020. We estimated abundance and identified preferred habitat characteristics using N-mixture models, and created suitable habitat maps based on data from surveys and remote sensing. Our estimate for Thailand was 578 (95% CI 391–854) individuals based on a density of 3.7 (95% CI 2.5–5.5) individuals/km² in 156 km² of suitable habitat. The giant nuthatch prefers dry forest with a large amount of mature native or planted pine Pinus kesiya and with a large tree basal area and an open canopy. Our estimate of suitable habitat remaining was less than previously reported and thus the population has probably decreased, although most of this habitat is within protected areas. Habitats for the species in Thailand have a stronger level of protection than in Myanmar and China, although habitat in China remains unquantified. We recommend further research in Myanmar and China, which may hold the majority of available habitat for the giant nuthatch. For long-term management, detailed study of the association of the giant nuthatch with pine plantations is required.
    Keywords Oryx ; Pinus kesiya ; Sitta ; canopy ; dry forests ; habitats ; population size ; Myanmar ; Thailand
    Language English
    Dates of publication 2022-03
    Size p. 195-201.
    Publishing place Cambridge University Press
    Document type Article
    ZDB-ID 417337-5
    ISSN 1365-3008 ; 0030-6053
    ISSN (online) 1365-3008
    ISSN 0030-6053
    DOI 10.1017/S0030605321000636
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

  2. Article: Determining the migration routes and wintering areas of Asian sparrowhawks through satellite telemetry

    Pierce, Andrew J. / Nualsri, Chukiat / Sutasha, Kaset / Round, Philip D.

    Global ecology and conservation. 2021 Nov., v. 31

    2021  

    Abstract: Understanding the movements and requirements of individual species within bird migration flyways is of crucial conservation importance, especially along the East Asian Flyway considering the immense human pressure on the environment and habitats. We ... ...

    Abstract Understanding the movements and requirements of individual species within bird migration flyways is of crucial conservation importance, especially along the East Asian Flyway considering the immense human pressure on the environment and habitats. We attached satellite transmitters to females of four Chinese Sparrowhawks Accipiter soloensis and four Japanese Sparrowhawks A. gularis mist-netted on their southward passage through Thailand. The Chinese Sparrowhawks wintered across a c. 3000 km-wide longitudinal span from Sumatra to Timor-Leste, spending 84–173 days on their wintering grounds before returning to breeding grounds in south and east China. Two were tracked for complete migration cycles of 14,688 and 9694 km, respectively. Three of four Japanese Sparrowhawks were tracked to wintering grounds in Sabah, Kalimantan, and the Bangka Belitung Islands where they spent 168–173 days before returning north. The Bangka Belitung winterer was tracked to presumed breeding grounds in Amurskiy Oblast, eastern Russia, traveling 7757 km in 53 days. Daily flights varied widely up to c. 800 and 382 km for Chinese and Japanese Sparrowhawk, respectively. With few individuals sampled, no significant differences were found within or between species, in the daily distances flown during southward or northward journeys. However, Japanese Sparrowhawks made fewer stopovers suggesting they traveled faster on northward migration than Chinese Sparrowhawks. Movements during the wintering and nesting periods were mostly confined to areas of less than 23 km², although one wintering Chinese Sparrowhawk used an area of over 600 km². Further work is needed to improve knowledge of the annual cycles of these and other migratory East Asian raptors and how they might differ among age- and sex-classes within species.
    Keywords Accipiter ; Borneo ; Russia ; humans ; migratory behavior ; satellites ; telemetry ; China ; East Timor ; Indonesia ; Thailand
    Language English
    Dates of publication 2021-11
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 2814786-8
    ISSN 2351-9894
    ISSN 2351-9894
    DOI 10.1016/j.gecco.2021.e01837
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

  3. Book ; Online: Collective identity, oppression, and the right to self-ascription

    Pierce, Andrew J

    2012  

    Abstract: Collective Identity, Oppression, and the Right to Self-Ascription argues that groups have an irreducibly collective right to determine the meaning of their shared group identity, and that such a right is especially important for historically oppressed ... ...

    Institution ebrary, Inc
    Author's details Andrew J. Pierce
    Abstract Collective Identity, Oppression, and the Right to Self-Ascription argues that groups have an irreducibly collective right to determine the meaning of their shared group identity, and that such a right is especially important for historically oppressed groups. It provides a novel approach to issues of identity politics, group rights, and racial identity, one which combines and develops the insights of contemporary critical theory and race theory, and will thus be of special interest to scholars in these fields
    Keywords Ethnicity ; Group identity ; Social groups
    Language English
    Size Online-Ressource (vii, 132 p)
    Publisher Lexington Books
    Publishing place Lanham, Md
    Document type Book ; Online
    Note Includes bibliographical references and index
    ISBN 9780739171905 ; 9780739171912 ; 0739171909 ; 0739171917
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

    More links

    Kategorien

  4. Article ; Online: The Gene Cluster Instability (GCI) Assay for Recombination.

    Killen, Michael W / Stults, Dawn M / Marco-Casanova, Paola / Pierce, Andrew J

    Methods in molecular biology (Clifton, N.J.)

    2020  Volume 2102, Page(s) 459–482

    Abstract: A newly developed method for quantitatively detecting genomic restructuring in cultured human cell lines as the result of recombination is presented: the "gene cluster instability" (GCI) assay. The assay is physiological in that it detects spontaneous ... ...

    Abstract A newly developed method for quantitatively detecting genomic restructuring in cultured human cell lines as the result of recombination is presented: the "gene cluster instability" (GCI) assay. The assay is physiological in that it detects spontaneous restructuring without the need for exogenous recombination-initiating treatments such as DNA damage. As an assay for genotoxicity, the GCI assay is complementary to well-established sister chromatid exchange (SCE) methods. Analysis of the U-2 OS osteosarcoma cell line is presented as an illustration of the method.
    MeSH term(s) Blotting, Southern ; Cell Line, Tumor ; DNA/drug effects ; DNA/genetics ; DNA/isolation & purification ; DNA Restriction Enzymes ; Electrophoresis, Agar Gel ; Genomic Instability ; Humans ; Multigene Family ; Mutagenicity Tests/methods ; Recombination, Genetic ; Workflow
    Chemical Substances DNA (9007-49-2) ; DNA Restriction Enzymes (EC 3.1.21.-)
    Language English
    Publishing date 2020-01-17
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-0223-2_26
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  5. Article ; Online: The Sister Chromatid Exchange (SCE) Assay.

    Stults, Dawn M / Killen, Michael W / Marco-Casanova, Paola / Pierce, Andrew J

    Methods in molecular biology (Clifton, N.J.)

    2020  Volume 2102, Page(s) 441–457

    Abstract: A fully optimized staining method for detecting sister chromatid exchanges in cultured cells is presented. The method gives reproducibly robust quantitative results. Sister chromatid exchange is a classic toxicology assay for genotoxicity and for ... ...

    Abstract A fully optimized staining method for detecting sister chromatid exchanges in cultured cells is presented. The method gives reproducibly robust quantitative results. Sister chromatid exchange is a classic toxicology assay for genotoxicity and for detecting alterations to the biochemistry underlying cellular homologous recombination. Growth of cells in the presence of 5'-bromo-deoxyuridine for two rounds of DNA replication followed by collecting metaphase spreads on glass slides, treatment with the UV-sensitive dye Hoechst 33258, long-wave UV light exposure, and Giemsa staining gives a permanent record of the exchanges.
    MeSH term(s) Azure Stains ; Biological Assay/methods ; Bisbenzimidazole ; Bromodeoxyuridine/metabolism ; Cells, Cultured ; Chromatids/drug effects ; Chromatids/metabolism ; Chromatids/radiation effects ; Chromosomes/drug effects ; Chromosomes/metabolism ; Chromosomes/radiation effects ; Homologous Recombination/drug effects ; Homologous Recombination/radiation effects ; Humans ; Metaphase/drug effects ; Metaphase/radiation effects ; Mutagenicity Tests/methods ; Sister Chromatid Exchange ; Workflow
    Chemical Substances Azure Stains ; Bromodeoxyuridine (G34N38R2N1) ; Bisbenzimidazole (LHQ7J5KV9B)
    Language English
    Publishing date 2020-01-17
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-0223-2_25
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  6. Article ; Online: Measuring recombination proficiency in mouse embryonic stem cells.

    Pierce, Andrew J / Jasin, Maria

    Methods in molecular biology (Clifton, N.J.)

    2014  Volume 1105, Page(s) 481–495

    Abstract: A method is presented to measure homologous recombination in mouse embryonic stem cells by both gene targeting and short-tract gene conversion of a double-strand break (DSB). A fluorescence-based reporter is first gene targeted to the Hprt locus in a ... ...

    Abstract A method is presented to measure homologous recombination in mouse embryonic stem cells by both gene targeting and short-tract gene conversion of a double-strand break (DSB). A fluorescence-based reporter is first gene targeted to the Hprt locus in a quantifiable way. A homing endonuclease expression vector is then introduced to generate a DSB, the repair of which is also quantifiable.
    MeSH term(s) Animals ; Blotting, Southern ; Cell Culture Techniques ; Cells, Cultured ; DNA/genetics ; DNA/isolation & purification ; DNA Breaks, Double-Stranded ; Embryonic Stem Cells/physiology ; Genes, Reporter ; Green Fluorescent Proteins/biosynthesis ; Green Fluorescent Proteins/genetics ; Hypoxanthine Phosphoribosyltransferase/biosynthesis ; Hypoxanthine Phosphoribosyltransferase/genetics ; Mice ; Plasmids/genetics ; Recombinational DNA Repair ; Sequence Analysis, DNA ; Transfection
    Chemical Substances Green Fluorescent Proteins (147336-22-9) ; DNA (9007-49-2) ; Hypoxanthine Phosphoribosyltransferase (EC 2.4.2.8)
    Language English
    Publishing date 2014
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-62703-739-6_34
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  7. Article ; Online: Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis.

    Marco-Casanova, Paola / Lukashchuk, Natalia / Lombardi, Benedetta / Munugalavadla, Veerendra / Frigault, Melanie M / Harrington, Elizabeth A / Barrett, J Carl / Pierce, Andrew J

    Journal of visualized experiments : JoVE

    2021  , Issue 169

    Abstract: Analysis of biomarkers in peripheral blood is becoming increasingly important in clinical trials to establish proof of mechanism to evaluate effects of treatment, and help guide dose and schedule setting of therapeutics. From a single blood draw, ... ...

    Abstract Analysis of biomarkers in peripheral blood is becoming increasingly important in clinical trials to establish proof of mechanism to evaluate effects of treatment, and help guide dose and schedule setting of therapeutics. From a single blood draw, peripheral blood mononuclear cells can be isolated and processed to analyze and quantify protein markers, and plasma samples can be used for the analysis of circulating tumor DNA, cytokines, and plasma metabolomics. Longitudinal samples from a treatment provide information on the evolution of a given protein marker, the mutational status and immunological landscape of the patient. This can only be achieved if the processing of the peripheral blood is carried out effectively in clinical sites and samples are properly preserved from the bedside to bench. Here, we present an optimized general-purpose protocol that can be implemented at clinical sites for obtaining PBMC pellets and plasma samples in multi-center clinical trials, that will enable clinical professionals in hospital laboratories to successfully provide high quality samples, regardless of their level of technical expertise. Alternative protocol variations are also presented that are optimized for more specific downstream analytical methods. We apply this protocol for studying protein biomarkers against DNA damage response (DDR) on X-ray irradiated blood to demonstrate the suitability of the approach in oncology settings where DDR drugs and/or radiotherapy have been practiced as well as in preclinical stages where mechanistic hypothesis testing is required.
    MeSH term(s) Biomarkers/blood ; Humans ; Leukocytes, Mononuclear/immunology ; Plasma/immunology
    Chemical Substances Biomarkers
    Language English
    Publishing date 2021-03-20
    Publishing country United States
    Document type Journal Article ; Video-Audio Media
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/60776
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  8. Article: Preparation of peripheral blood mononuclear cell pellets and plasma from a single blood draw at clinical trial sites for biomarker analysis

    Marco-Casanova, Paola / Lukashchuk, Natalia / Lombardi, Benedetta / Munugalavadla, Veerendra / Frigault, Melanie M. / Harrington, Elizabeth A. / Barrett, J. Carl / Pierce, Andrew J.

    Journal of visualized experiments. 2021 Mar. 20, , no. 169

    2021  

    Abstract: Analysis of biomarkers in peripheral blood is becoming increasingly important in clinical trials to establish proof of mechanism to evaluate effects of treatment, and help guide dose and schedule setting of therapeutics. From a single blood draw, ... ...

    Abstract Analysis of biomarkers in peripheral blood is becoming increasingly important in clinical trials to establish proof of mechanism to evaluate effects of treatment, and help guide dose and schedule setting of therapeutics. From a single blood draw, peripheral blood mononuclear cells can be isolated and processed to analyze and quantify protein markers, and plasma samples can be used for the analysis of circulating tumor DNA, cytokines, and plasma metabolomics. Longitudinal samples from a treatment provide information on the evolution of a given protein marker, the mutational status and immunological landscape of the patient. This can only be achieved if the processing of the peripheral blood is carried out effectively in clinical sites and samples are properly preserved from the bedside to bench. Here, we present an optimized general-purpose protocol that can be implemented at clinical sites for obtaining PBMC pellets and plasma samples in multi-center clinical trials, that will enable clinical professionals in hospital laboratories to successfully provide high quality samples, regardless of their level of technical expertise. Alternative protocol variations are also presented that are optimized for more specific downstream analytical methods. We apply this protocol for studying protein biomarkers against DNA damage response (DDR) on X-ray irradiated blood to demonstrate the suitability of the approach in oncology settings where DDR drugs and/or radiotherapy have been practiced as well as in preclinical stages where mechanistic hypothesis testing is required.
    Keywords DNA ; DNA damage ; X-radiation ; biomarkers ; clinical trials ; cytokines ; evolution ; hospitals ; landscapes ; metabolomics ; mononuclear leukocytes ; neoplasms ; patients ; radiotherapy
    Language English
    Dates of publication 2021-0320
    Size p. e60776.
    Publishing place Journal of Visualized Experiments
    Document type Article
    ZDB-ID 2259946-0
    ISSN 1940-087X
    ISSN 1940-087X
    DOI 10.3791/60776
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

  9. Article ; Online: CB307: A Dual Targeting Costimulatory Humabody VH Therapeutic for Treating PSMA-Positive Tumors.

    Archer, Sophie / Brailey, Phillip M / Song, Minjung / Bartlett, Phillip D / Figueiredo, Ines / Gurel, Bora / Guo, Christina / Brucklacher-Waldert, Verena / Thompson, H Lorraine / Akinwale, Jude / Boyle, Samantha E / Rossant, Christine / Birkett, Neil R / Pizzey, Julia / Maginn, Mark / Legg, James / Williams, Richard / Johnston, Colette M / Bland-Ward, Philip /
    de Bono, Johann S / Pierce, Andrew J

    Clinical cancer research : an official journal of the American Association for Cancer Research

    2024  Volume 30, Issue 8, Page(s) 1595–1606

    Abstract: Purpose: CD137 is a T- and NK-cell costimulatory receptor involved in consolidating immunologic responses. The potent CD137 agonist urelumab has shown clinical promise as a cancer immunotherapeutic but development has been hampered by on-target off- ... ...

    Abstract Purpose: CD137 is a T- and NK-cell costimulatory receptor involved in consolidating immunologic responses. The potent CD137 agonist urelumab has shown clinical promise as a cancer immunotherapeutic but development has been hampered by on-target off-tumor toxicities. A CD137 agonist targeted to the prostate-specific membrane antigen (PSMA), frequently and highly expressed on castration-resistant metastatic prostate cancer (mCRPC) tumor cells, could bring effective immunotherapy to this immunologically challenging to address disease.
    Experimental design: We designed and manufactured CB307, a novel half-life extended bispecific costimulatory Humabody VH therapeutic to elicit CD137 agonism exclusively in a PSMA-high tumor microenvironment (TME). The functional activity of CB307 was assessed in cell-based assays and in syngeneic mouse antitumor pharmacology studies. Nonclinical toxicology and toxicokinetic properties of CB307 were assessed in a good laboratory practice (GLP) compliant study in cynomolgus macaques.
    Results: CB307 provides effective CD137 agonism in a PSMA-dependent manner, with antitumor activity both in vitro and in vivo, and additional activity when combined with checkpoint inhibitors. A validated novel PSMA/CD137 IHC assay demonstrated a higher prevalence of CD137-positive cells in the PSMA-expressing human mCRPC TME with respect to primary lesions. CB307 did not show substantial toxicity in nonhuman primates and exhibited a plasma half-life supporting weekly clinical administration.
    Conclusions: CB307 is a first-in-class immunotherapeutic that triggers potent PSMA-dependent T-cell activation, thereby alleviating toxicologic concerns against unrestricted CD137 agonism.
    MeSH term(s) Male ; Humans ; Mice ; Animals ; Prostatic Neoplasms, Castration-Resistant/drug therapy ; Prostatic Neoplasms, Castration-Resistant/pathology ; Immunotherapy/methods ; Tumor Microenvironment
    Language English
    Publishing date 2024-04-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1225457-5
    ISSN 1557-3265 ; 1078-0432
    ISSN (online) 1557-3265
    ISSN 1078-0432
    DOI 10.1158/1078-0432.CCR-23-3052
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  10. Article ; Online: The sister chromatid exchange (SCE) assay.

    Stults, Dawn M / Killen, Michael W / Pierce, Andrew J

    Methods in molecular biology (Clifton, N.J.)

    2014  Volume 1105, Page(s) 439–455

    Abstract: A fully optimized staining method for detecting sister chromatid exchanges in cultured cells is presented. The method gives reproducibly robust quantitative results. Sister chromatid exchange is a classic toxicology assay for genotoxicity and for ... ...

    Abstract A fully optimized staining method for detecting sister chromatid exchanges in cultured cells is presented. The method gives reproducibly robust quantitative results. Sister chromatid exchange is a classic toxicology assay for genotoxicity and for detecting alterations to the biochemistry underlying cellular homologous recombination. Growth of cells in the presence of 5'-bromo-deoxyuridine for two rounds of DNA replication followed by collecting metaphase spreads on glass slides, treatment with the UV-sensitive dye Hoechst 33258, long-wave UV light exposure, and Giemsa staining gives a permanent record of the exchanges.
    MeSH term(s) Bromodeoxyuridine/metabolism ; DNA Replication ; HeLa Cells ; Humans ; K562 Cells ; Metaphase ; Recombinational DNA Repair ; Sister Chromatid Exchange ; Staining and Labeling
    Chemical Substances Bromodeoxyuridine (G34N38R2N1)
    Language English
    Publishing date 2014
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-62703-739-6_32
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

To top