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  1. Article ; Online: Attenuation of Lipopolysaccharide-Induced Inflammatory Responses through Inhibition of the NF-κB Pathway and the Increased NRF2 Level by a Flavonol-Enriched

    Notarte, Kin Israel R / Quimque, Mark Tristan J / Macaranas, Imee T / Khan, Abbas / Pastrana, Adriel M / Villaflores, Oliver B / Arturo, Hans Christian P / Pilapil Iv, Delfin Yñigo H / Tan, Sophia Morgan M / Wei, Dong-Qing / Wenzel-Storjohann, Arlette / Tasdemir, Deniz / Yen, Chia-Hung / Ji, Seon Yeong / Kim, Gi-Young / Choi, Yung Hyun / Macabeo, Allan Patrick G

    ACS omega

    2023  Volume 8, Issue 6, Page(s) 5377–5392

    Abstract: Pathologic hyperreactive inflammatory responses occur when there is excessive activation of a proinflammatory NF-κB pathway and a reduced cytoprotective NRF2 cascade. The noncytotoxic, highly selective COX-2 inhibitory flavonol-enriched butanol fraction ( ...

    Abstract Pathologic hyperreactive inflammatory responses occur when there is excessive activation of a proinflammatory NF-κB pathway and a reduced cytoprotective NRF2 cascade. The noncytotoxic, highly selective COX-2 inhibitory flavonol-enriched butanol fraction (UaB) from
    Language English
    Publishing date 2023-01-31
    Publishing country United States
    Document type Journal Article
    ISSN 2470-1343
    ISSN (online) 2470-1343
    DOI 10.1021/acsomega.2c06451
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Attenuation of Lipopolysaccharide-Induced Inflammatory Responses through Inhibition of the NF-κB Pathway and the Increased NRF2 Level by a Flavonol-Enriched n-Butanol Fraction from Uvaria alba

    Notarte, Kin Israel R. / Quimque, Mark Tristan J. / Macaranas, Imee T. / Khan, Abbas / Pastrana, Adriel M. / Villaflores, Oliver B. / Arturo, Hans Christian P. / Pilapil IV, Delfin Yñigo H. / Tan, Sophia Morgan M. / Wei, Dong-Qing / Wenzel-Storjohann, Arlette / Tasdemir, Deniz / Yen, Chia-Hung / Ji, Seon Yeong / Kim, Gi-Young / Choi, Yung Hyun / Macabeo, Allan Patrick G.

    2023  

    Abstract: Pathologic hyperreactive inflammatory responses occur when there is excessive activation of a proinflammatory NF-kappa B pathway and a reduced cytoprotective NRF2 cascade. The noncytotoxic, highly selective COX-2 inhibitory flavonol-enriched butanol ... ...

    Abstract Pathologic hyperreactive inflammatory responses occur when there is excessive activation of a proinflammatory NF-kappa B pathway and a reduced cytoprotective NRF2 cascade. The noncytotoxic, highly selective COX-2 inhibitory flavonol-enriched butanol fraction (UaB) from Uvaria alba (U. alba) was investigated for its inflammatory modulating potential by targeting NF-kappa B activation and NRF2 activity. Enzyme-linked immunosorbent assay was initially performed to measure levels of proinflammatory mediators [nitric oxide (NO), prostaglandin E2, and reactive oxygen species (ROS)] and cytokines [tumor necrosis factor-alpha (TNF-alpha), IL-1 beta, and IL-6], followed by reverse transcription-polymerase chain reaction and western blotting to determine mRNA and protein expression, respectively. Using immunofluorescence staining combined with western blot analysis, the activation of NF-kappa B was further investigated. NRF2 activity was also measured using a luciferase reporter assay. UaB abrogated protein and mRNA expressions of inducible nitric oxide synthase (iNOS), COX-2, TNF-alpha, IL-1 beta, and IL-6 in RAW 264.7 macrophages, thereby suppressing the production of proinflammatory mediators and cytokines. This was further validated when a concentration-dependent decrease in NO and ROS production was observed in zebrafish (Danio rerio) larvae. UaB also increased NRF2 activity in HaCaT/ARE cell line and attenuated NF-kappa B activation by inhibiting the nuclear translocation of transcription factor p65 in RAW 264.7 macrophages. Nontargeted LC- MS analysis of UaB revealed the presence of the flavonols quercitrin (1), quercetin (2), rutin (3), kaempferol (4), and kaempferol 3O-rutinoside (5). Molecular docking indicates that major flavonol aglycones have high affinity toward COX-2 NSAID-binding sites, TNF-alpha, and TNF-alpha converting enzyme, while the glycosylated flavonoids showed strong binding toward iNOS and IKK-all possessing dynamic stability when performing molecular dynamics simulations at 140 ns. This is the ...
    Subject code 610
    Language English
    Publishing date 2023-01-31
    Publisher ACS (American Chemical Society)
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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