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  1. Article ; Online: Applied autecology on the model of cationic proteins of neutrophil granulocytes of carnivores by adverse environmental effects

    Pimenov Nikolay / Permyakova Christina / Ivannikova Regina / Marzanova Saida

    E3S Web of Conferences, Vol 390, p

    2023  Volume 07006

    Abstract: The article presents studies related to the evaluation of the reaction of cationic proteins of neutrophil granulocytes as markers of environmental impact on the animal body on the example of carnivores (dogs). As is known, neutrophils are one of the ... ...

    Abstract The article presents studies related to the evaluation of the reaction of cationic proteins of neutrophil granulocytes as markers of environmental impact on the animal body on the example of carnivores (dogs). As is known, neutrophils are one of the first lines of defense of the macroorganism against many impacts, such as environmental pollution by various products of anthropogenic activity, bacterial and viral infections, etc. Therefore, taking into account the reaction of neutrophil cationic proteins to adverse environmental effects may be of interest for the early detection of immunological changes. We found that the level of neutrophil cationic proteins in stray dogs was significantly higher than in domestic dogs. This fact may indicate a longer adaptation process of the body of homeless animals in response to changes in the environmental situation and requires further more detailed study.
    Keywords Environmental sciences ; GE1-350
    Language English
    Publishing date 2023-01-01T00:00:00Z
    Publisher EDP Sciences
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Prognosis of septic complications and the outcome of the disease according to the saps score scale in dogs with leptospirosis

    Laptev Sergey / Pimenov Nikolay / Marzanova Saida / Permyakova Kristina / Ivannikova Regina

    BIO Web of Conferences, Vol 71, p

    2023  Volume 01077

    Abstract: The article presents the results of the analysis of clinical and laboratory data of 24 dogs in order to create a convenient model for the formation of the prognosis of septic complications and the outcome of the disease. The use of the SAPS scale ... ...

    Abstract The article presents the results of the analysis of clinical and laboratory data of 24 dogs in order to create a convenient model for the formation of the prognosis of septic complications and the outcome of the disease. The use of the SAPS scale simplifies the assessment of physiological disorders caused by the clinical manifestation of SOURCE, MODS and CARS and allows you to make a reliable forecast of possible complications in dogs with leptospirosis. So, with complications, there is a slight decrease in the number of red blood cells and hemoglobin, a slight increase in ESR. The level of AST, ALT has been increased. An increase in the level of bilirubin, urea and creatinine was revealed. The level of rod-shaped neutrophils is increased, and segmented neutrophils are lowered. The prognosis of septic complications in dogs with leptospirosis on the SAPS scale allows us to assess the degree of danger of physiological disorders caused by the clinical manifestations of SIRS, MODS and CARS in the shortest possible time. should leave 8 mm of space above the abstract and 10 mm after the abstract. The heading Abstract should be typed in bold 9-point Arial. The body of the abstract should be typed in normal 9-point Times in a single paragraph, immediately following the heading. The text should be set to 1 line spacing.
    Keywords Microbiology ; QR1-502 ; Physiology ; QP1-981 ; Zoology ; QL1-991
    Subject code 630
    Language English
    Publishing date 2023-01-01T00:00:00Z
    Publisher EDP Sciences
    Document type Article ; Online
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  3. Article: Salinity-induced succession of phototrophic communities in a southwestern Siberian soda lake during the solar activity cycle.

    Samylina, Olga S / Kosyakova, Anastasia I / Krylov, Artem A / Sorokin, Dimitry Yu / Pimenov, Nikolay V

    Heliyon

    2024  Volume 10, Issue 4, Page(s) e26120

    Abstract: A variety of lakes located in the dry steppe area of southwestern Siberia are exposed to rapid climatic changes, including intra-century cycles with alternating dry and wet phases driven by solar activity. As a result, the salt lakes of that region ... ...

    Abstract A variety of lakes located in the dry steppe area of southwestern Siberia are exposed to rapid climatic changes, including intra-century cycles with alternating dry and wet phases driven by solar activity. As a result, the salt lakes of that region experience significant fluctuations in water level and salinity, which have an essential impact on the indigenous microbial communities. But there are few microbiological studies that have analyzed this impact, despite its importance for understanding the functioning of regional water ecosystems. This work is a retrospective study aimed at analyzing how solar activity-related changes in hydrological regime affect phototrophic microbial communities using the example of the shallow soda lake Tanatar VI, located in the Kulunda steppe (Altai Region, Russia, southwestern Siberia). The main approach used in this study was the comparison of hydrochemical and microscopic data obtained during annual field work with satellite and solar activity data for the 12-year observation period (2011-2022). The occurrence of 33 morphotypes of cyanobacteria, two key morphotypes of chlorophytes, and four morphotypes of anoxygenic phototrophic bacteria was analyzed due to their easily recognizable morphology. During the study period, the lake surface changed threefold and the salinity changed by more than an order of magnitude, which strongly correlated with the phases of the solar activity cycles. The periods of high (2011-2014; 100-250 g/L), medium (2015-2016; 60 g/L), extremely low (2017-2020; 13-16 g/L), and low (2021-2022; 23-34 g/L) salinity with unique biodiversity of phototrophic communities were distinguished. This study shows that solar activity cycles determine the dynamics of the total salinity of a southwestern Siberian soda lake, which in turn determines the communities and microorganisms that will occur in the lake, ultimately leading to cyclical changes in alternative states of the ecosystem (dynamic stability).
    Language English
    Publishing date 2024-02-17
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2024.e26120
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  4. Article: Halanaerobium polyolivorans

    Boltyanskaya, Yulia / Zhilina, Tatjana / Grouzdev, Denis / Detkova, Ekaterina / Pimenov, Nikolay / Kevbrin, Vadim

    Microorganisms

    2023  Volume 11, Issue 9

    Abstract: A search for the microorganisms responsible for the anaerobic degradation of osmoprotectants in soda lakes resulted in the isolation of a novel halophilic and alkalitolerant strain, designated Z- ... ...

    Abstract A search for the microorganisms responsible for the anaerobic degradation of osmoprotectants in soda lakes resulted in the isolation of a novel halophilic and alkalitolerant strain, designated Z-7514
    Language English
    Publishing date 2023-09-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11092325
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  5. Article ; Online: Urbanization as an ecological phenomenon

    Lomskov Mikhail A. / Pimenov Nikolay V. / Ivannikova Regina F. / Konovalov Alexander M. / Tishchenko Alexander S.

    E3S Web of Conferences, Vol 390, p

    2023  Volume 07002

    Abstract: In this article authors discuss the main ecological aspects of the urbanization process. In particular, such phenomena as anthropogenic transformation of the environment, which is the basis of all further transformations forming urban areas, are analyzed. ...

    Abstract In this article authors discuss the main ecological aspects of the urbanization process. In particular, such phenomena as anthropogenic transformation of the environment, which is the basis of all further transformations forming urban areas, are analyzed. In addition, the processes of anthropogenic change of organisms, for example, cultivation and domestication, are considered. Unfortunately, in modern science, the process of urbanization is usually considered from economic, social, and cultural positions, often without affecting the environmental component. This fact is a significant omission, especially in the context of an increase in the number of natural landscapes transformed by human.
    Keywords Environmental sciences ; GE1-350
    Language English
    Publishing date 2023-01-01T00:00:00Z
    Publisher EDP Sciences
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: A Novel View of the Diversity of Anoxygenic Phototrophic Bacteria Inhabiting the Chemocline of Meromictic Karst Lakes.

    Gorlenko, Vladimir / Savvichev, Alexander / Kadnikov, Vitaly / Rusanov, Igor / Beletsky, Alexey / Zakharova, Elena / Kostrikina, Nadezhda / Sigalevich, Pavel / Veslopolova, Elena / Pimenov, Nikolay

    Microorganisms

    2023  Volume 12, Issue 1

    Abstract: The rates of oxygenic and anoxygenic photosynthesis, the microorganisms responsible for these processes, and the hydrochemical characteristics of the sulfide-containing karst lakes, Black Kichier and Big Kichier (Mari El Republic), were investigated. In ... ...

    Abstract The rates of oxygenic and anoxygenic photosynthesis, the microorganisms responsible for these processes, and the hydrochemical characteristics of the sulfide-containing karst lakes, Black Kichier and Big Kichier (Mari El Republic), were investigated. In these lakes, a plate of anoxygenic phototrophic bacteria (APB) is formed at the upper boundary of sulfide occurrence in the water. The phototrophic community of the chemocline zone was analyzed using a combination of high-throughput sequencing of the 16S rRNA gene fragments and light and electron microscopic techniques. Green-colored
    Language English
    Publishing date 2023-12-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms12010013
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  7. Article: The Baltic Sea methane pockmark microbiome: The new insights into the patterns of relative abundance and ANME niche separation

    Iasakov, Timur R. / Kanapatskiy, Timur A. / Toshchakov, Stepan V. / Korzhenkov, Aleksei A. / Ulyanova, Marina O. / Pimenov, Nikolay V.

    Marine environmental research. 2022 Jan., v. 173

    2022  

    Abstract: Pockmarks are important “pumps”, which are believed to play a significant role in the global methane cycling and harboring a unique assemblage of very diverse prokaryotes. This study reports the results of massive sequencing of the 16S rRNA gene V4 ... ...

    Abstract Pockmarks are important “pumps”, which are believed to play a significant role in the global methane cycling and harboring a unique assemblage of very diverse prokaryotes. This study reports the results of massive sequencing of the 16S rRNA gene V4 hypervariable regions for the samples from thirteen pockmark horizons (the Baltic Sea) collected at depths from 0 to 280 cm below seafloor (cmbsf) and the rates of microbially mediated anaerobic oxidation of methane (AOM) and sulfate reduction (SR). Altogether, 76 bacterial and 12 archaeal phyla were identified, 23 of which were candidate divisions. Of the total obtained in the pockmark sequences, 84.3% of them were classified as Bacteria and 12.4% as Archaea; 3.3% of the sequences were assigned to unknown operational taxonomic units (OTUs). Members of the phyla Planctomycetota, Chloroflexota, Desulfobacterota, Caldatribacteriota, Acidobacteriota and Proteobacteria predominated across all horizons, comprising 58.5% of the total prokaryotic community. These phyla showed different types of patterns of relative abundance. Analysis of AOM-SR-mediated prokaryotes abundance and biogeochemical measurements revealed that ANME-2a-2b subcluster was predominant in sulfate-rich upper horizons (including sulfate-methane transition zone (SMTZ)) and together with sulfate-reducing bacterial group SEEP-SRB1 had a primary role in AOM coupled to SR. At deeper sulfate-depleted horizons ANME-2a-2b shifted to ANME-1a and ANME-1b which alone mediated AOM or switch to methanogenic metabolism. Shifting of the ANME subclusters depending on depth reflect a tendency for niche separation in these groups. It was shown that the abundance of Caldatribacteriota and organohalide-respiring Dehalococcoidia (Chloroflexota) exhibited a strong correlation with AOM rates. This is the first detailed study of depth profiles of prokaryotic diversity, patterns of relative abundance, and ANME niche separation in the Baltic Sea pockmark microbiomes sheds light on assembly of prokaryotes in a pockmark.
    Keywords Archaea ; Dehalococcoidia ; Proteobacteria ; ecological differentiation ; genes ; metabolism ; methane ; methanogens ; microbiome ; oxidation ; prokaryotic cells ; research ; sulfates ; Baltic Sea
    Language English
    Dates of publication 2022-01
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1502505-6
    ISSN 1879-0291 ; 0141-1136
    ISSN (online) 1879-0291
    ISSN 0141-1136
    DOI 10.1016/j.marenvres.2021.105533
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  8. Article ; Online: Proteinivorax hydrogeniformans sp. nov., an anaerobic, haloalkaliphilic bacterium fermenting proteinaceous compounds with high hydrogen production.

    Boltyanskaya, Yulia / Detkova, Ekaterina / Pimenov, Nikolay / Kevbrin, Vadim

    Antonie van Leeuwenhoek

    2018  Volume 111, Issue 2, Page(s) 275–284

    Abstract: A search for the organisms responsible for the degradation of biomass of primary producers in Tanatar lakes resulted in the isolation of a novel anaerobic, haloalkaliphilic microorganism, strain Z- ... ...

    Abstract A search for the organisms responsible for the degradation of biomass of primary producers in Tanatar lakes resulted in the isolation of a novel anaerobic, haloalkaliphilic microorganism, strain Z-710
    MeSH term(s) Bacteria, Anaerobic/metabolism ; Clostridiales/chemistry ; Clostridiales/classification ; Clostridiales/metabolism ; Clostridiales/physiology ; Fermentation ; Hydrogen/metabolism ; Metabolomics/methods ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA
    Chemical Substances RNA, Ribosomal, 16S ; Hydrogen (7YNJ3PO35Z)
    Language English
    Publishing date 2018-02
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 214861-4
    ISSN 1572-9699 ; 0003-6072
    ISSN (online) 1572-9699
    ISSN 0003-6072
    DOI 10.1007/s10482-017-0949-9
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  9. Article: CRISPR Screening: Molecular Tools for Studying Virus–Host Interactions

    Chulanov, Vladimir / Kostyusheva, Anastasiya / Brezgin, Sergey / Ponomareva, Natalia / Gegechkori, Vladimir / Volchkova, Elena / Pimenov, Nikolay / Kostyushev, Dmitry

    Viruses. 2021 Nov. 11, v. 13, no. 11

    2021  

    Abstract: CRISPR/Cas is a powerful tool for studying the role of genes in viral infections. The invention of CRISPR screening technologies has made it possible to untangle complex interactions between the host and viral agents. Moreover, whole-genome and pathway- ... ...

    Abstract CRISPR/Cas is a powerful tool for studying the role of genes in viral infections. The invention of CRISPR screening technologies has made it possible to untangle complex interactions between the host and viral agents. Moreover, whole-genome and pathway-specific CRISPR screens have facilitated identification of novel drug candidates for treating viral infections. In this review, we highlight recent developments in the fields of CRISPR/Cas with a focus on the use of CRISPR screens for studying viral infections and identifying new candidate genes to aid development of antivirals.
    Keywords antiviral agents ; development aid ; genes ; host-pathogen relationships ; screening ; technology ; viruses
    Language English
    Dates of publication 2021-1111
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v13112258
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  10. Article: Treatment and monitoring of children and adolescents with hepatitis C in Russia: Results from a multi-centre survey on policy and practice.

    Malik, Farihah / Chulanov, Vladimir / Pimenov, Nikolay / Fomicheva, Anastasia / Lundin, Rebecca / Levina, Nataliia / Thorne, Claire / Turkova, Anna / Indolfi, Giuseppe

    Journal of virus eradication

    2022  Volume 8, Issue 1, Page(s) 100063

    Abstract: Background: The Russian Federation has the largest paediatric hepatitis C virus (HCV) disease burden in the World Health Organization European region with an estimated 118,000 children living with HCV viraemia. Direct-acting antivirals (DAAs) have been ... ...

    Abstract Background: The Russian Federation has the largest paediatric hepatitis C virus (HCV) disease burden in the World Health Organization European region with an estimated 118,000 children living with HCV viraemia. Direct-acting antivirals (DAAs) have been available for adults in Russia since 2015 and approved for treatment of adolescents aged ≥12 years since 2019. We evaluated DAA availability and uptake for HCV treatment of children and adolescents and clinical practices on diagnosis and management of paediatric HCV in Russia.
    Methods: A survey was distributed to regional ministries of health in 85 administrative regions during September 2020. The survey consisted of 22 items collecting data on: type of facility, aggregate patient characteristics, HCV testing practices for children and pregnant women and HCV management and treatment practices for children.
    Results: Survey responses were received from 37 of the 85 regions in Russia (response rate 44%). 2159 children and adolescents with chronic HCV were in follow-up; 1089 (50%) were female. Of 2080 children with available data on age-groups, 134 (6%) were <3 years, 336 (16%) 3-<6 years, 718 (35%) 6-<12 years and 892 (43%) 12-<18 years. 134 (15%) of 892 adolescents ≥12 years received DAAs, 96 (72%) glecaprevir/pibrentasvir, 26 (19%) sofosbuvir, 8 (6%) daclatasvir and 4 (3%) sofosbuvir/ledipasvir.
    Conclusions: This study provides a baseline of DAA uptake in early stages of rollout for children and adolescents. The use of DAAs for treatment of adolescents in Russia presents a unique opportunity for HCV micro-elimination in this population.
    Language English
    Publishing date 2022-02-03
    Publishing country England
    Document type Journal Article
    ZDB-ID 2868549-0
    ISSN 2055-6659 ; 2055-6640
    ISSN (online) 2055-6659
    ISSN 2055-6640
    DOI 10.1016/j.jve.2022.100063
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