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  1. Article ; Online: Derivation of the MUSIe002-A human embryonic stem cell line

    Sujittra Khampang / Chuti Laowtammathron / Chanchao Lorthongpanich / Phatchanat Klaihmon / Pimjai Chingsuwanrote / Roungsin Choavaratana / Suphadtra Phornwilardsiri / Ketsara Sitthirit / Pimonwan Srisook / Yaowalak U-pratya / Surapol Issaragrisil

    Stem Cell Research, Vol 59, Iss , Pp 102660- (2022)

    2022  

    Abstract: The MUSIe002-A cell line was established from in vitro fertilization of human sperm and oocytes donated for research with informed consent. This cell line exhibited normal human embryonic stem cell (hESC) characteristics, including typical cell ... ...

    Abstract The MUSIe002-A cell line was established from in vitro fertilization of human sperm and oocytes donated for research with informed consent. This cell line exhibited normal human embryonic stem cell (hESC) characteristics, including typical cell morphology, expression of all pluripotent stem cell markers, and potential to differentiate into three germ layers. A karyotyping analysis revealed 46 XY chromosome and cells that did not have mycoplasma contamination. MUSIe002-A represents a valuable unlimited cell source and is of potential interest for human in vitro stem cell based-models, genetic modifications, and stem cell-based therapy of human disease.
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Generation of a serine/threonine-protein kinase LATS1 gene-edited iPSC MUSIi012-A-3

    Chanchao Lorthongpanich / Chuti Laowtammathron / Nittaya Jiamvoraphong / Pimonwan Srisook / Pimjai Chingsuwanrote / Phatchanat Klaihmon / Supaporn Waeteekul / Yaowalak U-pratya / Surapol Issaragrisil

    Stem Cell Research, Vol 48, Iss , Pp 101950- (2020)

    2020  

    Abstract: In mammals, there are a number of kinases, including serine/threonine-protein kinase LATS1, that act as a core kinase of the Hippo pathway and that negatively regulate the Hippo effector protein YAP and its paralog TAZ. Using CRISPR/Cas9 technology, we ... ...

    Abstract In mammals, there are a number of kinases, including serine/threonine-protein kinase LATS1, that act as a core kinase of the Hippo pathway and that negatively regulate the Hippo effector protein YAP and its paralog TAZ. Using CRISPR/Cas9 technology, we established a stable LATS1 knockdown (LATS1-KD) iPSC from the MUSIi012-A cell line. The LATS1-KD iPSC MUSIi012-A-3 that was developed maintained both the normal karyotype and the pluripotent phenotype, and retained the ability to differentiate into all three embryonic germ layers.
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2020-10-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Derivation of a MUSIi012-A iPSCs from mobilized peripheral blood stem cells

    Chuti Laowtammathron / Pimonwan Srisook / Pimjai Chingsuwanrote / Nittaya Jiamvoraphong / Supaporn Waeteekul / Papussorn Terbto / Yaowalak U-Pratya / Chanchao Lorthongpanich / Surapol Issaragrisil

    Stem Cell Research, Vol 41, Iss , Pp - (2019)

    2019  

    Abstract: CD34+ cells were isolated from mobilized peripheral blood of a healthy donor and reprogrammed by nucleofection with episomal plasmids carrying l-MYC, LIN28, OCT4, SOX2, KLF4, EBNA-1, and shRNA against p53. The obtained MUSIi012-A cell line maintained the ...

    Abstract CD34+ cells were isolated from mobilized peripheral blood of a healthy donor and reprogrammed by nucleofection with episomal plasmids carrying l-MYC, LIN28, OCT4, SOX2, KLF4, EBNA-1, and shRNA against p53. The obtained MUSIi012-A cell line maintained the pluripotent phenotype, the ability to differentiate into all three germ layers, and a normal karyotype.
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2019-12-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Derivation of human embryonic stem cell line MUSIe001-A from an embryo with homozygous α0-thalassemia (SEA deletion)

    Chuti Laowtammathron / Pimjai Chingsuwanrote / Roungsin Choavaratana / Suphadtra Phornwilardsiri / Ketsara Sitthirit / Chidchanok Kaewjunun / Orawan Makemaharn / Papussorn Terbto / Supaporn Waeteekul / Chanchao Lorthongpanich / Yaowalak U-pratya / Pimonwan Srisook / Pakpoom Kheolamai / Surapol Issaragrisil

    Stem Cell Research, Vol 43, Iss , Pp - (2020)

    2020  

    Abstract: MUSIe001-A cell line was derived from a Southeast Asian (SEA) type deletion α0-thalassemia embryo. The SEA deletion embryo was donated for research with informed consent. This cell line shows normal hESC morphology, expresses all pluripotent markers, and ...

    Abstract MUSIe001-A cell line was derived from a Southeast Asian (SEA) type deletion α0-thalassemia embryo. The SEA deletion embryo was donated for research with informed consent. This cell line shows normal hESC morphology, expresses all pluripotent markers, and has the potential to differentiate into all three germ layers in vitro and in vivo. The MUSIe001-A line has normal karyotype and is free from mycoplasma contamination. PCR analysis confirmed the MUSIe001-A cell line to be a SEA type deletion. MUSIe001-A is a valuable proof of principle model for gene therapy that will facilitate the development of new treatments for affected foetuses.
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2020-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: YAP-depleted iPSC MUSIi012-A-2 maintained all normal stem cell characteristics

    Chanchao Lorthongpanich / Chuti Laowtammathron / Nittaya Jiamvoraphong / Pimonwan Srisook / Pimjai Chingsuwanrote / Phatchanat Klaihmon / Nattaya Damkham / Papussorn Terbto / Supaporn Waeteekul / Yaowalak U-pratya / Surapol Issaragrisil

    Stem Cell Research, Vol 43, Iss , Pp - (2020)

    2020  

    Abstract: Yes-associated protein (YAP) is an important transcriptional coactivator in the Hippo signaling pathway. Using CRISPR/Cas9 technology, we established a stable YAP-knockdown (YAP-KD) induced pluripotent stem cell (iPSC) from the MUSIi012-A cell line. The ... ...

    Abstract Yes-associated protein (YAP) is an important transcriptional coactivator in the Hippo signaling pathway. Using CRISPR/Cas9 technology, we established a stable YAP-knockdown (YAP-KD) induced pluripotent stem cell (iPSC) from the MUSIi012-A cell line. The YAP-KD iPSC MUSIi012-A-2 maintained the pluripotent phenotype, the ability to differentiate into all three embryonic germ layers, and it maintained the normal karyotype. Keywords: YAP, Hippo signaling pathway, iPSC
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2020-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Generation of human induced pluripotent stem cell line carrying SCN5AC2204>T Brugada mutation (MUSli009-A-1) introduced by CRISPR/Cas9-mediated genome editing

    Paweorn Angsutararux / Sudjit Luanpitpong / Pimjai Chingsuwanrote / Kantpitchar Supraditaporn / Supaporn Waeteekul / Papussorn Terbto / Chanchao Lorthongpanich / Chuti Laowtammathron / Yaowalak U-Pratya / Surapol Issaragrisil

    Stem Cell Research, Vol 41, Iss , Pp - (2019)

    2019  

    Abstract: Human induced pluripotent stem cells (hiPSCs) derived from dermal fibroblasts having wild type (WT) SCN5A were engineered by CRISPR/Cas9-mediated genome editing to harbor a specific point mutation (C2204>T) in SCN5A, which results in a substitution of ... ...

    Abstract Human induced pluripotent stem cells (hiPSCs) derived from dermal fibroblasts having wild type (WT) SCN5A were engineered by CRISPR/Cas9-mediated genome editing to harbor a specific point mutation (C2204>T) in SCN5A, which results in a substitution of the WT alanine by valine at codon 735 (A735V). The established MUSli009-A-1 hiPSC line has a homozygous C2204>T mutation on exon 14 of SCN5A that was confirmed by DNA sequencing analysis. The cells exhibited normal karyotype, expressed pluripotent markers and retained its capability to differentiate into three germ layers. The cardiomyocytes derived from this line would be a useful model for investigating cardiac channelopathy.
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2019-12-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: High-efficiency derivation of human embryonic stem cell lines using a culture system with minimized trophoblast cell proliferation

    Chuti Laowtammathron / Pimjai Chingsuwanrote / Roungsin Choavaratana / Suphadtra Phornwilardsiri / Ketsara Sitthirit / Chidchanok Kaewjunun / Orawan Makemaharn / Papussorn Terbto / Supaporn Waeteekul / Chanchao Lorthongpanich / Yaowalak U-pratya / Pimonwan Srisook / Pakpoom Kheolamai / Surapol Issaragrisil

    Stem Cell Research & Therapy, Vol 9, Iss 1, Pp 1-

    2018  Volume 10

    Abstract: Abstract Background Due to their extensive self-renewal and multilineage differentiation capacity, human embryonic stem cells (hESCs) have great potential for studying developmental biology, disease modeling, and developing cell replacement therapy. The ... ...

    Abstract Abstract Background Due to their extensive self-renewal and multilineage differentiation capacity, human embryonic stem cells (hESCs) have great potential for studying developmental biology, disease modeling, and developing cell replacement therapy. The first hESC line was generated in 1998 by culturing inner cell mass (ICM) cells isolated from human blastocysts using an immunosurgery technique. Since then, many techniques including mechanical ICM isolation, laser dissection, and whole embryo culture have been used to derive hESC lines. However, the hESC derivation efficiency remains low, usually less than 50%, and it requires a large number of human embryos to derive a significant number of hESC lines. Due to a shortage of and restricted access to human embryos, a novel approach with better hESC derivation efficiency is badly needed to decrease the number of embryos used. Methods We hypothesized that the low hESC derivation efficiency might be due to extensive proliferation of trophoblast (TE) cells which could interfere with ICM proliferation. We therefore developed a methodology to minimize TE cell proliferation by culturing ICM in a feeder-free system for 3 days before transferring them onto feeder cells. Results This minimized trophoblast cell proliferation (MTP) technique could be successfully used to derive hESCs from normal, abnormal, and frozen–thawed embryos with better derivation efficiency of more than 50% (range 50–100%; median 70%). Conclusions We successfully developed a better hESC derivation methodology using the “MTP” culture system. This methodology can be effectively used to derive hESCs from both normal and abnormal embryos under feeder-free conditions with higher efficiency when compared with other methodologies. With this methodology, large-scale production of clinical-grade hESCs is feasible.
    Keywords Human embryonic stem cells ; Embryo ; Clinical grade ; Trophoblast cells ; Medicine (General) ; R5-920 ; Biochemistry ; QD415-436
    Language English
    Publishing date 2018-05-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article: Partial Substitution of Glucose with Xylitol Suppressed the Glycolysis and Selectively Inhibited the Proliferation of Oral Cancer Cells

    Trachootham, Dunyaporn / Pimjai Chingsuwanrote / Penkhair Yoosadiang / Duangsamon Mekkriangkrai / Tanapat Ratchawong / Nicha Buraphacheep / Siripa Kijanukul / Sumrit Saekhow / Orawan Pongpitchayadej / Kittituch Vongvachvasin / Paknisa Sittikornpaiboon / Siriporn Tuntipopipat

    Nutrition and cancer. 2017 Aug. 18, v. 69, no. 6

    2017  

    Abstract: Suitable diet for cancer survivors remains an unresolved challenge. Increased glucose utilization is a hallmark of various cancers. Therefore, alternative carbohydrate supplying normal tissue but retarding cancer growth is needed. This study investigated ...

    Abstract Suitable diet for cancer survivors remains an unresolved challenge. Increased glucose utilization is a hallmark of various cancers. Therefore, alternative carbohydrate supplying normal tissue but retarding cancer growth is needed. This study investigated the effect of sugar alcohols on the proliferation of oral cancer cells compared to nontransformed cells and explored the mechanism. Six oral squamous cell carcinoma (CAL-27, FaDu, SCC4, SCC9, SCC15, and SCC25) and one nontransformed oral keratinocyte (OKF6/TERT2) lines were cultured in media containing 1 mg/ml glucose and 5.8 mg/ml xylitol or sorbitol, yielding equal energy input to control group (4.5 mg/ml glucose). Partial substitution of glucose with sugar alcohols especially xylitol significantly suppressed proliferation of oral cancer but not nontransformed cells. Despite the addition of isocaloric quantities of the sugars, cancer cells exposed to low glucose plus xylitol had retarded ATP generation and decreased activity of phosphofructokinase (PFK), the rate-limiting enzyme in glycolysis. Furthermore, D-xylulose, its key metabolic intermediate, enhanced the anticancer effect of xylitol. These findings suggested a selective anticancer activity of xylitol and the potential mechanism involving inhibition of glucose utilization. Partial substitution of glucose with xylitol may be a proper nutrient for oral cancer survivors, deserving further investigation in animal and clinical settings.
    Keywords adenosine triphosphate ; animals ; antineoplastic activity ; diet ; energy ; glucose ; glycolysis ; keratinocytes ; mouth neoplasms ; neoplasm cells ; phosphofructokinases ; sorbitol ; squamous cell carcinoma ; xylitol
    Language English
    Dates of publication 2017-0818
    Size p. 862-872.
    Publishing place Taylor & Francis
    Document type Article
    ZDB-ID 2025822-7
    ISSN 1532-7914 ; 0163-5581
    ISSN (online) 1532-7914
    ISSN 0163-5581
    DOI 10.1080/01635581.2017.1339097
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: Stir-Fry Chicken with Green Curry Suppresses Inflammatory Gene Expression by Lipopolysaccharide-Induced Murine Macrophages

    Somsri Charoenkiatkul / Channarong Muangnoi / Pimjai Chingsuwanrote / Phawachaya Praengamthanachoti / Siriporn Tuntipopipat / Saovaros Svasti

    Food and Nutrition Sciences , Vol 02, Iss 07, Pp 770-

    2011  Volume 779

    Abstract: Inflammatory mediators produced during inflammatory response play an important role on pathological development of several chronic diseases. Although several dietary plants exhibited anti-inflammatory property, their impacts as a whole food has been ... ...

    Abstract Inflammatory mediators produced during inflammatory response play an important role on pathological development of several chronic diseases. Although several dietary plants exhibited anti-inflammatory property, their impacts as a whole food has been rarely reported. The aim of the present study is to assess anti-inflammatory activity of an ethanol extract from a whole food namely “ready to eat stir-fry chicken with green curry” consisting of green curry paste, big egg plant, pea egg plant, red chili, kaffer lime and sweet basil as plant-based ingredients. The food extract at 55 - 220 μg/ml was incubated with RAW264.7 murine macrophage cells prior to stimulation with lipopolysaccharide (LPS) for 24 h. Inflammatory mediators [(inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF-α) and interleukine-6 (IL-6)] mRNA and protein were determined by RT-PCR, immunoblot and ELISA respectively. The modulation mechanism by the food extract was observed by measuring the phosphorylated form of mitogen activated protein kinases (MAPKs) and inhibitor kappa B alpha (IκB-α). The ready to eat stir-fry chicken with green curry extract significantly suppressed LPS-induced iNOS, COX-2, IL-6 and TNF-α gene expression in a dose-depend- ent manner without cytotoxicity. The suppressive effect was modulated partly by inhibiting phosphorylation of MAPKs and IκB-α. These results indicate that spices and vegetables in a complex diet still possess strong anti-inflammatory activities which warrant confirming such activities to ameliorate the pathogenesis of inflammatory-associated chronic diseases in vivo.
    Keywords Stir-Fry Chicken with Green Curry ; Anti-Inflammation ; Lipopolysaccharide ; RAW264.7 Murine Macrophages ; Nutrition. Foods and food supply ; TX341-641 ; Home economics ; TX1-1110 ; Technology ; T ; DOAJ:Nutrition and Food Sciences ; DOAJ:Agriculture and Food Sciences
    Language English
    Publishing date 2011-09-01T00:00:00Z
    Publisher Scientific Research Publishing
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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