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  1. Article ; Online: MicroRNA-29a Manifests Multifaceted Features to Intensify Radiosensitivity, Escalate Apoptosis, and Revoke Cell Migration for Palliating Radioresistance-Enhanced Cervical Cancer Progression

    Pei-Chin Chuang / Ping-Tsung Chen / Chih-Chi Wang / Wen-Hong Su / Yen-Hao Chen / Eng-Yen Huang

    International Journal of Molecular Sciences, Vol 23, Iss 5524, p

    2022  Volume 5524

    Abstract: Radioresistance remains a major clinical challenge in cervical cancer therapy and results in tumor relapse and metastasis. Nevertheless, the detailed mechanisms are still largely enigmatic. This study was conducted to elucidate the prospective impacts of ...

    Abstract Radioresistance remains a major clinical challenge in cervical cancer therapy and results in tumor relapse and metastasis. Nevertheless, the detailed mechanisms are still largely enigmatic. This study was conducted to elucidate the prospective impacts of microRNA-29a ( miR-29a ) on the modulation of radioresistance-associated cervical cancer progression. Herein, we established two pairs of parental wild-type (WT) and radioresistant (RR) cervical cancer cells (CaSki and C33A), and we found that constant suppressed miR-29a, but not miR-29b/c, was exhibited in RR-clones that underwent a dose of 6-Gy radiation treatment. Remarkably, radioresistant clones displayed low radiosensitivity, and the reduced apoptosis rate resulted in augmented surviving fractions, measured by the clonogenic survival curve assay and the Annexin V/Propidium Iodide apoptosis assay, respectively. Overexpression of miR-29a effectively intensified the radiosensitivity and triggered the cell apoptosis in RR-clones. In contrast, suppressed miR-29a modestly abridged the radiosensitivity and abolished the cell apoptosis in WT-clones. Hence, ectopically introduced miR-29a into RR-clones notably attenuated the wound-healing rate and cell migration, whereas reduced miR-29a aggravated cell mobilities of WT-clones estimated via the in vitro wound-healing assay and time-lapse recording assay. Notably, we further established the in vivo short-term lung locomotion metastasis model in BALB/c nude mice, and we found that increased lung localization was shown after tail-vein injection of RR-CaSki cells compared to those of WT-CaSki cells. Amplified miR-29a significantly eliminated the radioresistance-enhanced lung locomotion. Our data provide evidence suggesting that miR-29a is a promising microRNA signature in radioresistance of cervical cancer cells and displays multifaceted innovative roles involved in anti-radioresistance, escalated apoptosis, and anti-cell migration/metastasis. Amalgamation of a nucleoid-based strategy ( miR-29a ) together with ...
    Keywords microRNA-29a ; radioresistance ; apoptosis ; cell migration ; cervical cancer progression ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 610
    Language English
    Publishing date 2022-05-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Predictive Value of the Pretreatment Neutrophil-to-Lymphocyte Ratio in Head and Neck Squamous Cell Carcinoma

    Miao-Fen Chen / Ming-Shao Tsai / Wen-Cheng Chen / Ping-Tsung Chen

    Journal of Clinical Medicine, Vol 7, Iss 10, p

    2018  Volume 294

    Abstract: This study assessed the significance of the neutrophil-to-lymphocyte ratio (NLR) in head and neck squamous cell carcinoma (HNSCC), and the relationships of the NLR with the aldehyde dehydrogenase 1 (ALDH1) level in tumors and the proportion of myeloid- ... ...

    Abstract This study assessed the significance of the neutrophil-to-lymphocyte ratio (NLR) in head and neck squamous cell carcinoma (HNSCC), and the relationships of the NLR with the aldehyde dehydrogenase 1 (ALDH1) level in tumors and the proportion of myeloid-derived suppressor cells (MDSCs) in the peripheral circulation. In total, 227 HNSCC patients who had received curative treatment at our hospital were enrolled into the present study. The NLR of each HNSCC patient before treatment was calculated. The associations of NLR with various clinicopathological parameters and prognoses were then examined. In addition, correlations between the proportion of MDSCs and level of ALDH1 with the NLR were assessed. Our data revealed that an elevated NLR was significantly correlated with the risk of developing locoregional recurrence and with a reduced overall survival in HNSCC patients. Multivariate analyses revealed that the NLR pretreatment and surgical resection were significantly correlated with the rate of treatment failure and the overall survival rate in HNSCC patients. Furthermore, the levels of ALDH1 in tumors and MDSCs in the peripheral circulation were significantly correlated with the prognosis of HNSCC, and the NLR was positively correlated with MDSC levels in the circulation and ALDH1 staining intensity in tumor specimens. In conclusion, the NLR has power in predicting the expression of ALDH1 in tumors, the circulating level of MDSCs, and the prognosis in HNSCC. We suggest that the NLR is an important biomarker that can assist the clinician and patient in making informed decisions regarding treatment options for HNSCC patients.
    Keywords head and neck squamous cell carcinoma (HNSCC) ; neutrophil-to-lymphocyte ratio (NLR) ; myeloid-derived suppressor cells (MDSC) ; aldehyde dehydrogenase 1 (ALDH1) ; prognosis ; Medicine ; R
    Subject code 616
    Language English
    Publishing date 2018-09-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Intravenous Ondansetron plus Intravenous Dexamethasone with Different Ondansetron Dosing Schedules during Multiple Cycles of Cisplatin-based Chemotherapy

    Chuang-Chi Liaw / Ping-Tsung Chen

    Biomedical Journal , Vol 31, Iss 02, Pp 167-

    2008  Volume 174

    Abstract: Background: This study examined whether different ondansetron dosing schedules plusdexamethasone influenced antiemetic efficacy during multiple cycles of cisplatin-based chemotherapy (CT). Antiemetic activities between previous CTand subsequent cycles ... ...

    Abstract Background: This study examined whether different ondansetron dosing schedules plusdexamethasone influenced antiemetic efficacy during multiple cycles of cisplatin-based chemotherapy (CT). Antiemetic activities between previous CTand subsequent cycles were compared.Methods: The cross-over study involved 424 patients. Arm A, three doses of 8 mgondansetron given intravenously (IV) at 4-hourly intervals plus dexamethasone20 mg IV at the start of CT, followed by dexamethasone 5 mg IV every12 hours. Arm B, as arm A but the three doses of 8 mg ondansetron weregiven at 24-hourly intervals. For those with complete protection from emesisin both arms, a single dose of 8 mg ondansetron (arm C) was tried during thefollowing CT. Once complete protection of emesis could not be maintained,arm A regimens were administered in the subsequent cycles of CT.Results: There were 384, 377 and 147 patients in arm A, arm B and arm C, respectively.Complete control of acute and delayed nausea/vomiting obtained inarm A were 91.4%/94.8% and 59.6%/70.1%, and in arm B were90.4%/92.3% and 61.3% 72.7%. There was no significant difference inantiemetic efficacy between both arms. Decreased incidence of and delayedonset of nausea on day 2 were observed in arm B (p = 0.002). The emeticseverity during previous CT correlated significantly with those of the subsequentCT. The complete control of nausea/vomiting was maintained in81.6%/72.1% of arm C patients during the following 3rd-6th cycles of CT.Conclusion: No difference in antiemetic efficacy was shown when a triple 8 mg dose ofondansetron was given at 4-hourly intervals or at 24-hourly intervals.However, the latter improved nausea on day 2. A single 8 mg dose ofondansetron can maintain antiemetic efficacy in the majority of completeresponders in arm A and arm B.
    Keywords chemotherapy ; cisplatin ; emesis ; 5-HT3 antagonist ; dexamethasone ; ondansetron ; Medicine (General) ; R5-920 ; Medicine ; R ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
    Subject code 610
    Language English
    Publishing date 2008-04-01T00:00:00Z
    Publisher Chang Gung University
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Author Correction

    Jrhau Lung / Ming‑Szu Hung / Yu‑Ching Lin / Yuan Yuan Jiang / Yu‑Hung Fang / Ming‑Shian Lu / Ching‑Chuan Hsieh / Chia‑Siu Wang / Feng‑Che Kuan / Chang‑Hsien Lu / Ping‑Tsung Chen / Chieh‑Mo Lin / Yen‑Li Chou / Chin‑Kuo Lin / Tsung‑Ming Yang / Fen Fen Chen / Paul Yann Lin / Meng‑Jer Hsieh / Ying Huang Tsai

    Scientific Reports, Vol 11, Iss 1, Pp 1-

    A highly sensitive and specific real-time quantitative PCR for BRAF V600E/K mutation screening

    2021  Volume 1

    Abstract: An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    Abstract An amendment to this paper has been published and can be accessed via a link at the top of the paper.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2021-06-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Impacts of demographic and laboratory parameters on key hematological indices in an adult population of southern Taiwan

    Ming-Chung Wang / Cih-En Huang / Meng-Hung Lin / Yao-Hsu Yang / Chang-Hsien Lu / Ping-Tsung Chen / Yu-Ying Wu / Hsing-Yi Tsou / Chia-Chen Hsu / Chih-Cheng Chen

    PLoS ONE, Vol 13, Iss 8, p e

    A cohort study.

    2018  Volume 0201708

    Abstract: Studies in Caucasians have shown that values of hematological indices could be affected by a wide variety of factors. However, parallel work in other ethnical populations, particularly from the Asia-Pacific region, is lacking. Therefore, we designed this ...

    Abstract Studies in Caucasians have shown that values of hematological indices could be affected by a wide variety of factors. However, parallel work in other ethnical populations, particularly from the Asia-Pacific region, is lacking. Therefore, we designed this study to explore the association between clinical/laboratory parameters and hemogram levels. Adult individuals who came to our hospital for health exams were screened. Information on demographics and laboratory profiles was obtained. We analyzed the impacts of these parameters on the variation of hemogram. Overall, 26,497 adults were included in the current analysis after excluding those with abnormal hemogram. Multivariate regression analysis showed increasing age and male gender negatively affected the number of platelets, whereas a higher serum apolipoprotein B level was associated with an elevated platelet count. Gender and serum albumin level were the major determinants of variation in hemoglobin level. A modestly increased white cell count was seen in men as well as individuals with elevated apolipoprotein B levels, but it was inversely correlated with changes in age and serum albumin levels. Conversely, some variables, although statistically significantly associated with the hematological indices, only provided a trivial explanation for the heterogeneity observed. We further established predictive models for the approximate estimation of hematological indices in healthy adults. Our data indicate that age, gender, and serum levels of apolipoprotein B and albumin affect hematological indices in various ways. We also demonstrate that variation in hemogram could be successfully predicted by a number of clinical and laboratory parameters.
    Keywords Medicine ; R ; Science ; Q
    Subject code 630
    Language English
    Publishing date 2018-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: A highly sensitive and specific real-time quantitative PCR for BRAF V600E/K mutation screening

    Jrhau Lung / Ming-Szu Hung / Yu-Ching Lin / Yuan Yuan Jiang / Yu-Hung Fang / Ming-Shian Lu / Ching-Chuan Hsieh / Chia-Siu Wang / Feng-Che Kuan / Chang-Hsien Lu / Ping-Tsung Chen / Chieh-Mo Lin / Yen-Li Chou / Chin-Kuo Lin / Tsung-Ming Yang / Fen Fen Chen / Paul Yann Lin / Meng-Jer Hsieh / Ying Huang Tsai

    Scientific Reports, Vol 10, Iss 1, Pp 1-

    2020  Volume 9

    Abstract: Abstract Mutations that lead to constitutive activation of key regulators in cellular processes are one of the most important drivers behind vigorous growth of cancer cells, and are thus prime targets in cancer treatment. BRAF V600E mutation transduces ... ...

    Abstract Abstract Mutations that lead to constitutive activation of key regulators in cellular processes are one of the most important drivers behind vigorous growth of cancer cells, and are thus prime targets in cancer treatment. BRAF V600E mutation transduces strong growth and survival signals for cancer cells, and is widely present in various types of cancers including lung cancer. A combination of BRAF inhibitor (dabrafenib) and MEK inhibitor (trametinib) has recently been approved and significantly improved the survival of patients with advanced NSCLC harboring BRAF V600E/K mutation. To improve the detection of BRAF V600E/K mutation and investigate the incidence and clinicopathological features of the mutation in lung cancer patients of southern Taiwan, a highly sensitive and specific real-time quantitative PCR (RT-qPCR) method, able to detect single-digit copies of mutant DNA, was established and compared with BRAF V600E-specific immunohistochemistry. Results showed that the BRAF V600E mutation was present at low frequency (0.65%, 2/306) in the studied patient group, and the detection sensitivity and specificity of the new RT-qPCR and V600E-specific immunohistochemistry both reached 100% and 97.6%, respectively. Screening the BRAF V600E/K mutation with the RT-qPCR and V600E-specific immunohistochemistry simultaneously could help improve detection accuracy.
    Keywords Medicine ; R ; Science ; Q
    Subject code 610
    Language English
    Publishing date 2020-10-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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