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Article ; Online: Efficacy and Safety of Edoxaban in Cancer-Associated Venous Thromboembolism: A Real World Retrospective Study.

Grifoni, Elisa / Baroncelli, Andrea / Pinto, Gabriele / Cosentino, Eleonora / Micheletti, Irene / Signorini, Ira / Panigada, Grazia / Landini, Giancarlo / Masotti, Luca

TH open : companion journal to thrombosis and haemostasis

2022 Volume 6, Issue 2, Page(s) e99–e106

Abstract: ... ...

Abstract	Introduction
Language	English
Publishing date	2022-03-01
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2901738-5
ISSN	2512-9465 ; 2567-3459
ISSN (online)	2512-9465
ISSN	2567-3459
DOI	10.1055/a-1783-9744
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Article ; Online: Galdieria sulphuraria

Ambrosino, Annalisa / Chianese, Annalisa / Zannella, Carla / Piccolella, Simona / Pacifico, Severina / Giugliano, Rosa / Franci, Gianluigi / De Natale, Antonino / Pollio, Antonino / Pinto, Gabriele / De Filippis, Anna / Galdiero, Massimiliano

Marine drugs

2023 Volume 21, Issue 7

Abstract: In the last decades, the interest in bioactive compounds derived from natural sources including bacteria, fungi, plants, and algae has significantly increased. It is well-known that aquatic or terrestrial organisms can produce, in special conditions, ... ...

Abstract	In the last decades, the interest in bioactive compounds derived from natural sources including bacteria, fungi, plants, and algae has significantly increased. It is well-known that aquatic or terrestrial organisms can produce, in special conditions, secondary metabolites with a wide range of biological properties, such as anticancer, antioxidant, anti-inflammatory, and antimicrobial activities. In this study, we focused on the extremophilic microalga
MeSH term(s)	Humans ; Antiviral Agents/chemistry ; Viruses ; Coronavirus Infections ; Rhodophyta/metabolism ; Plant Extracts/pharmacology
Chemical Substances	Antiviral Agents ; Plant Extracts
Language	English
Publishing date	2023-06-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2175190-0
ISSN	1660-3397 ; 1660-3397
ISSN (online)	1660-3397
ISSN	1660-3397
DOI	10.3390/md21070383
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Article: Correction to: Novel qPCR probe systems for the characterization of subaerial biofilms on stone monuments

del Mondo, Angelo / de Natale, Antonino / Pinto, Gabriele / Pollio, Antonino

Annals of microbiology. 2019 Oct., v. 69, no. 10

2019

Abstract: PURPOSE: A deep survey of biodeteriogen microorganisms reported on stone monuments in Europe has been performed based on the available literature dating back to over 30 years. The aim of the present study is to obtain accurate oligos for the ... ...

Abstract	PURPOSE: A deep survey of biodeteriogen microorganisms reported on stone monuments in Europe has been performed based on the available literature dating back to over 30 years. The aim of the present study is to obtain accurate oligos for the characterization of subaerial biofilms on the basis of the most comprehensive collection of reports and case studies regarding subaerial biofilms, with particular regard to phototrophic and non-phototrophic bacteria, eukaryotic algae and molds. METHODS: The obtained lists for eukaryotic algae, phototrophic and non-phototrophic bacteria and fungi were sorted by Genera and corresponding sequences in triplicate were downloaded by nucleotide database Genbank for a number of selected barcoding markers. On the basis of collected bibliometric diversity, multiple nucleotide alignments were produced and primers were designed for a qPCR assay. RESULT: Primers were designed on conserved regions flanking a variable region, specific for each of the studied groups of microorganisms. Standard curve for absolute quantification relative to each group were determined for four markers. Then, variable regions in the alignments were used to design fluorescent internal probes for qPCR aimed for a multiplex reaction in which relative abundance can be determined. CONCLUSION: The authors propose this kind of cost-effective approach in the study of biofilms for the estimation of algae, molds and bacteria both for direct in situ analysis and in vitro simulation.
Keywords	DNA barcoding ; algae ; bacteria ; biofilm ; case studies ; cost effectiveness ; fluorescence ; genetic databases ; molds (fungi) ; monuments ; quantitative polymerase chain reaction ; rocks ; surveys ; Europe
Language	English
Dates of publication	2019-10
Size	p. 1097-1106.
Publishing place	Springer International Publishing
Document type	Article
Note	Published Erratum
ZDB-ID	2143009-3
ISSN	1869-2044 ; 1590-4261
ISSN (online)	1869-2044
ISSN	1590-4261
DOI	10.1007/s13213-019-01480-9
Database	NAL-Catalogue (AGRICOLA)

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Article: Survey of relevant taxonomic groups for the design of qPCR primers and internal fluorescent probes for whole characterization of subaerial biofilm

Del Mondo, Angelo / De Natale, Antonino / Pinto, Gabriele / Pollio, Antonino

Annals of microbiology. 2019 Oct., v. 69, no. 10

2019

Abstract: PURPOSE: A deep survey of biodeteriogen microorganisms reported on stone monuments in Europe has been performed based on the available literature dating back to over 30 years. METHODS: The obtained lists for eukaryotic algae, phototrophic and non- ... ...

Abstract	PURPOSE: A deep survey of biodeteriogen microorganisms reported on stone monuments in Europe has been performed based on the available literature dating back to over 30 years. METHODS: The obtained lists for eukaryotic algae, phototrophic and non-phototrophic bacteria, and fungi were sorted by Genera, and corresponding sequences in triplicate were downloaded by nucleotide database Genbank for a number of selected barcoding markers. On the basis of collected bibliometric diversity, multiple nucleotide alignments were produced and primers were designed for a qPCR assay. The aim of the present study was to obtain accurate oligos for the characterization of subaerial biofilms on the basis of the most comprehensive collection of reports and case studies regarding subaerial biofilms, with particular regard to phototrophic and non-phototrophic bacteria, eukaryotic algae, and molds. RESULT: Primers were designed on conserved regions flanking a variable region, specific for each of the studied groups of microorganisms. Standard curve for absolute quantification relative to each group were determined for four markers. Then, variable regions in the alignments were used to design fluorescent internal probes for qPCR aimed for a multiplex reaction in which relative abundance can be determined. CONCLUSION: The authors propose this kind of cost-effective approach in the study of biofilms for the estimation of algae, molds, and bacteria both for direct in situ analysis and in vitro simulation.
Keywords	DNA barcoding ; algae ; bacteria ; biofilm ; case studies ; cost effectiveness ; fluorescence ; fluorescent dyes ; genetic databases ; molds (fungi) ; monuments ; quantitative polymerase chain reaction ; rocks ; surveys ; Europe
Language	English
Dates of publication	2019-10
Size	p. 1087-1096.
Publishing place	Springer International Publishing
Document type	Article
ZDB-ID	2143009-3
ISSN	1869-2044 ; 1590-4261
ISSN (online)	1869-2044
ISSN	1590-4261
DOI	10.1007/s13213-019-01461-y
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Article ; Online: Post-Stroke Detection of Subclinical Paroxysmal Atrial Fibrillation in Patients With Embolic Stroke of Undetermined Source in the Real World Practice: The Empoli ESUS Atrial Fibrillation (E 2 AF) Study.

Grifoni, Elisa / Baldini, Giulia / Baldini, Mariella / Pinto, Gabriele / Micheletti, Irene / Madonia, Elisa M / Cosentino, Eleonora / Bartolozzi, Maria L / Bertini, Elisabetta / Dei, Alessandro / Signorini, Ira / Giannoni, Sara / Del Rosso, Attilio / Prisco, Domenico / Guidi, Leonello / Masotti, Luca

The neurologist

2023 Volume 28, Issue 1, Page(s) 25–31

Abstract: Background: Subclinical paroxysmal atrial fibrillation (AF) is one of the main occult causative mechanisms of embolic stroke of undetermined source (ESUS). Aim of this study was to identify AF predictors, and to develop a score to predict the ... ...

Abstract	Background: Subclinical paroxysmal atrial fibrillation (AF) is one of the main occult causative mechanisms of embolic stroke of undetermined source (ESUS). Aim of this study was to identify AF predictors, and to develop a score to predict the probability of AF detection in ESUS. Methods: We retrospectively analyzed ESUS patients undergoing 2-week external electrocardiographic monitoring. Patients with and without AF detection were compared. On the basis of multivariate analysis, predictors of AF were identified and used to develop a predictive score, which was then compared with other existing literature scores. Results: Eighty-two patients, 48 females, mean age±SD 72±10 years, were included. In 36 patients (43.9%) AF was detected. The frequency of age 75 years or above and arterial hypertension, and the median CHA 2 DS 2 -VASc score were significantly higher in patients with AF compared with those without. National Institutes of Health Stroke Scale (NIHSS) score ≥8 was the only independent variable associated with AF detection. We derived the Empoli ESUS-AF (E 2 AF) score (NIHSS ≥8 5 points, arterial hypertension 3 points, age 75 years or above 2 points, age 65 to 74 years 1 point, history of coronary/peripheral artery disease 1 point, left atrial enlargement 1 point, posterior lesion 1 point, cortical or cortical-subcortical lesion 1 point), whose predictive power in detecting AF was good (area under the curve: 0.746, 95% confidence interval: 0.638-0.836) and higher than that of CHA 2 DS 2 -VASc and other scores. Conclusions: In our study NIHSS score ≥8 was the only independent predictor of post-ESUS-AF detection. The E 2 AF score appears to have a good predictive power for detecting AF. External validations are required.
MeSH term(s)	Aged ; Female ; Humans ; Atrial Fibrillation/complications ; Atrial Fibrillation/diagnosis ; Embolic Stroke/complications ; Hypertension/complications ; Hypertension/diagnosis ; Retrospective Studies ; Risk Factors ; Stroke/complications ; Stroke/diagnosis ; Male
Language	English
Publishing date	2023-01-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	1361380-7
ISSN	2331-2637 ; 1074-7931
ISSN (online)	2331-2637
ISSN	1074-7931
DOI	10.1097/NRL.0000000000000440
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Article: Efficacy and Safety of Edoxaban in Cancer-Associated Venous Thromboembolism: A Real World Retrospective Study

Grifoni, Elisa / Baroncelli, Andrea / Pinto, Gabriele / Cosentino, Eleonora / Micheletti, Irene / Signorini, Ira / Panigada, Grazia / Landini, Giancarlo / Masotti, Luca

TH Open

2022 Volume 06, Issue 02, Page(s) e99–e106

Abstract: Introduction: Few data exist on the use of edoxaban in cancer-associated venous thromboembolism (VTE) outside of clinical trials. Aim of this study was to evaluate the characteristics and outcomes of these patients in a real world clinical setting.: ... ...

Abstract	Introduction: Few data exist on the use of edoxaban in cancer-associated venous thromboembolism (VTE) outside of clinical trials. Aim of this study was to evaluate the characteristics and outcomes of these patients in a real world clinical setting. Methods: We retrospectively analyzed the characteristics of patients with cancer-associated VTE who were prescribed edoxaban. Follow-up at 3, 6, and 12 months was performed: VTE recurrences, bleedings, mortality, cancer progression and treatment, edoxaban interruption and its reason were assessed. Results: Fifty-four patients, 38 females (70.4%), mean age 71 ± 14 years, were enrolled. In 38 patients (70.4%), the episode of VTE was the first one, in 28 (51.8%) it was an isolated deep vein thrombosis (DVT), in 13 (24.1%) a pulmonary embolism (PE) associated with DVT, in 13 (24.1%) an isolated PE. Median time between cancer and VTE diagnosis was 6 (interquartile range [IQR] 2–47) months. Median time between VTE diagnosis and edoxaban prescription was 36 (IQR 7–117) days. At 3, 6, and 12 months the incidence of all-cause mortality was 16.6, 22.2, and 38.8%, that of VTE recurrence 1.8, 1.8, and 3.7%, and that of major bleeding 7.4, 9.2, and 12.9%, respectively. No bleeding was fatal. Of the 33 patients alive at 12 months, 32 (96.9%) were still on edoxaban therapy, in seven (21.2%) cancer was in progression. Conclusion: Our study, conducted on a real world population of patients with cancer-associated VTE, confirms the results of randomized controlled clinical trials, and supports the use of edoxaban as effective and safe treatment in this context.
Keywords	cancer ; venous thromboembolism ; bleeding ; edoxaban ; low molecular weight heparin
Language	English
Publishing date	2022-03-01
Publisher	Georg Thieme Verlag KG
Publishing place	Stuttgart ; New York
Document type	Article
ZDB-ID	2901738-5
ISSN	2512-9465 ; 2567-3459
ISSN (online)	2512-9465
ISSN	2567-3459
DOI	10.1055/a-1783-9744
Database	Thieme publisher's database

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Article: Nutrient removal efficiency of green algal strains at high phosphate concentrations.

Moreno Osorio, Jairo Hernan / Del Mondo, Angelo / Pinto, Gabriele / Pollio, Antonino / Frunzo, Luigi / Lens, Piet Nicolaas Luc / Esposito, Giovanni

Water science and technology : a journal of the International Association on Water Pollution Research

2020 Volume 80, Issue 10, Page(s) 1832–1843

Abstract: The effects of autotrophic and mixotrophic conditions on microalgae growth and nutrient removal efficiency from synthetic wastewater by different microalgae were investigated. Although several studies have demonstrated the suitability of microalgae ... ...

Abstract	The effects of autotrophic and mixotrophic conditions on microalgae growth and nutrient removal efficiency from synthetic wastewater by different microalgae were investigated. Although several studies have demonstrated the suitability of microalgae technologies for ammonia-rich wastewater treatment, only a few have been used for treatment of phosphate-rich industrial wastewaters. In this work, six microalgae were cultivated in batch mode in a growth medium with a high phosphate concentration (0.74 Mm PO
MeSH term(s)	Biomass ; Chlorella vulgaris ; Microalgae ; Nitrogen ; Nutrients ; Phosphates ; Waste Water
Chemical Substances	Phosphates ; Waste Water ; Nitrogen (N762921K75)
Language	English
Publishing date	2020-03-06
Publishing country	England
Document type	Journal Article
ZDB-ID	764273-8
ISSN	1996-9732 ; 0273-1223
ISSN (online)	1996-9732
ISSN	0273-1223
DOI	10.2166/wst.2019.431
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Article ; Online: Biofilm architecture on different substrates of an Oculatella subterranea (Cyanobacteria) strain isolated from Pompeii archaeological site (Italy).

Del Mondo, Angelo / Pinto, Gabriele / Carbone, Dora Allegra / Pollio, Antonino / De Natale, Antonino

Environmental science and pollution research international

2018 Volume 25, Issue 26, Page(s) 26079–26089

Abstract: The Cyanobacterium Oculatella subterranea Zammit, Billi, Albertano inhabits hypogea and stone caves and is a pioneer of different stone substrata. In this study, a strain isolated from the House of Marco Castricio (Archaeological Park of Pompeii, Italy) ... ...

Abstract	The Cyanobacterium Oculatella subterranea Zammit, Billi, Albertano inhabits hypogea and stone caves and is a pioneer of different stone substrata. In this study, a strain isolated from the House of Marco Castricio (Archaeological Park of Pompeii, Italy) was identified by a polyphasic approach and used for an in vitro colonization test to verify the influence of the substrate on the biofilm architecture. Fine structure of O. subterranea microbial mats was revealed as well as filaments orientation toward light source. This aim has been achieved through confocal laser scanner microscope microscopy and computer image analysis. Moreover, bioreceptivity of five different substrates, commonly retrieved in archaeological sites of Campania, was assessed for O. subterranea. Our results show that the three-dimensional structure of O. subterranea microbial mats is poorly affected by physical and geochemical features of substrates: in fact, the porous architecture of its biofilm was preserved, independently of the materials. On the other hand, the area/perimeter ratio relative to the O. subterranea growth on tuff, brick, and porphyry showed significant differences, indicating dissimilar levels of bioreceptivity of the three substrates.
MeSH term(s)	Biofilms/growth & development ; Cyanobacteria/growth & development ; Cyanobacteria/isolation & purification ; Italy ; Light ; Microscopy, Confocal
Language	English
Publishing date	2018-07-03
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-018-2643-5
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Article ; Online: Weathering of a Roman Mosaic-A Biological and Quantitative Study on In Vitro Colonization of Calcareous Tesserae by Phototrophic Microorganisms.

Marasco, Addolorata / Nocerino, Simona / Pinto, Gabriele / Pollio, Antonino / Trojsi, Giorgio / Natale, Antonino De

PloS one

2016 Volume 11, Issue 10, Page(s) e0164487

Abstract: The potential impact of cyanobacteria and microalgae on the weathering of calcareous tesserae from a Roman mosaic of the II Century CE has been followed through in vitro experiments. Laboratory tests were carried out by inoculating mosaic tiles with ... ...

Abstract	The potential impact of cyanobacteria and microalgae on the weathering of calcareous tesserae from a Roman mosaic of the II Century CE has been followed through in vitro experiments. Laboratory tests were carried out by inoculating mosaic tiles with single strains of Cyanobacteria or Chlorophyta to evaluate the roles of pioneer phototrophic microrganism on the resulting architecture of biofilms. The interaction between tesserae and strains was assessed at the whole substratum and micrometer scales, by image analysis and Confocal Laser Scanning (CLS) microscopy, respectively. The biofilm surface coverage on each tessera varied from 19% (Fischerella ambigua) to 97% (Microcoleus autumnalis). Cyanobacteria showed a better growth on calcareous tesserae, whereas the only green alga attaining a superficial coverage higher than 50% was Coelastrella rubescens. CLS microscopy evidenced two different types of spatial arrangement of the phototrophic organisms on the tesserae, that were defined as compact or porous, respectively. In the first one was measured a reduced number of empty spaces between cells or filaments, whereas in the second type, a reticulate texture allowed the presence of numerous empty volumes. The colonization processes observed are an intrinsic characteristic of each strain. We have proposed a colonization index IC as a sensible tool to describe, in a quantitative way, the pioneering attitude of each photosynthetic microorganism to colonize lithic substrates under laboratory conditions.
MeSH term(s)	Biofilms ; Cyanobacteria/growth & development ; Cyanobacteria/physiology ; Microalgae/growth & development ; Microalgae/physiology
Language	English
Publishing date	2016
Publishing country	United States
Document type	Journal Article
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0164487
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Article ; Online: Cryptic dispersal of Cyanidiophytina (Rhodophyta) in non-acidic environments from Turkey.

Iovinella, Manuela / Eren, Ayla / Pinto, Gabriele / Pollio, Antonino / Davis, Seth J / Cennamo, Paola / Ciniglia, Claudia

Extremophiles : life under extreme conditions

2018 Volume 22, Issue 5, Page(s) 713–723

Abstract: Cyanidiophytina are a group of polyextremophilic red algae with a worldwide, but discontinuous colonization. They are restricted to widely dispersed hot springs, geothermal habitats, and also some human-altered environments. Cyanidiophytina are ... ...

Abstract	Cyanidiophytina are a group of polyextremophilic red algae with a worldwide, but discontinuous colonization. They are restricted to widely dispersed hot springs, geothermal habitats, and also some human-altered environments. Cyanidiophytina are predominant where pH is prohibitive for the majority of eukaryotes (pH 0.5-3). Turkey is characterized by areas rich in volcanic activity separated by non-volcanic areas. Here we show that Cyanidiophycean populations are present in thermal baths located around Turkey on neutral/alkaline soils. All known genera and species within Cyanidiophytina were detected in Turkey, including Galdieria phlegrea, recorded up to now only in Italian Phlegrean Fields. By phylogenetic analyses, Turkish G. sulphuraria strains are monophyletic with Italian and Icelandic strains, and with Russian G. daedala strains. G. maxima from Turkey clustered with Icelandic, Kamchatka, and Japanese populations. The discovery of Cyanidiophytina in non-acidic Turkish soils raises new questions about the ecological boundaries of these extremophilic algae. This aids in the understanding of the dispersal abilities and distribution patterns of this ecologically and evolutionarily interesting group of algae.
MeSH term(s)	Acids/analysis ; Biodiversity ; Hydrothermal Vents/chemistry ; Hydrothermal Vents/microbiology ; Phylogeny ; Rhodophyta/classification ; Rhodophyta/genetics ; Rhodophyta/physiology ; Turkey
Chemical Substances	Acids
Language	English
Publishing date	2018-05-19
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1481278-2
ISSN	1433-4909 ; 1431-0651
ISSN (online)	1433-4909
ISSN	1431-0651
DOI	10.1007/s00792-018-1031-x
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