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Article ; Online: Sex moderates the association between quantitative sensory testing and acute and chronic pain after total knee/hip arthroplasty.

Paredes, Ana C / Arendt-Nielsen, Lars / Almeida, Armando / Pinto, Patrícia R

2024 Volume 24, Issue 1

Abstract: Objectives: Acute postsurgical pain (APSP) may persist over time and become chronic. Research on predictors for APSP and chronic postsurgical pain (CPSP) has produced inconsistent results. This observational study aimed to analyze psychological and ... ...

Abstract	Objectives: Acute postsurgical pain (APSP) may persist over time and become chronic. Research on predictors for APSP and chronic postsurgical pain (CPSP) has produced inconsistent results. This observational study aimed to analyze psychological and psychophysical variables associated with APSP and CPSP after total knee or hip arthroplasty, and to explore the role of sex. Methods: Assessments were conducted before surgery, 48 h, and 3 months postsurgery, including questionnaires (sociodemographic, pain related, and psychological) and quantitative sensory testing (QST). Hierarchical linear regression models analyzed potential predictors of APSP and CPSP, and moderation analyses evaluated the role of sex. Results: The study included 63 participants undergoing total knee (34, 54%) or hip (29, 46%) arthroplasty. Thirty-one (49.2%) were female and 32 (50.8%) were male. APSP (48 h) was associated with impaired conditioned pain modulation (CPM) ( Conclusions: Specific QST parameters could identify patients at risk for high-intensity APSP and CPSP, with sex as a moderator. This has important clinical implications for patient care, paving the way for developing tailored preventive pain management strategies.
MeSH term(s)	Humans ; Male ; Female ; Pain, Postoperative/psychology ; Pain, Postoperative/diagnosis ; Chronic Pain/psychology ; Arthroplasty, Replacement, Knee/adverse effects ; Arthroplasty, Replacement, Hip/adverse effects ; Aged ; Middle Aged ; Sex Factors ; Acute Pain/psychology ; Pain Measurement/methods
Language	English
Publishing date	2024-04-29
Publishing country	Germany
Document type	Journal Article ; Observational Study ; Research Support, Non-U.S. Gov't
ZDB-ID	2515451-5
ISSN	1877-8879 ; 1877-8860
ISSN (online)	1877-8879
ISSN	1877-8860
DOI	10.1515/sjpain-2024-0004
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Article ; Online: Uniparental Disomy as a Mechanism for Combined Oxidative Phosphorylation Deficiency Associated with MRPS34 Gene.

Soares, Marta P / Travessa, André M / Custódio, Sónia / Pereira, Carla / Pinto, Patrícia / Sousa, Ana Berta

Endocrine, metabolic & immune disorders drug targets

2024

Abstract: Introduction: Mitochondrial oxidative phosphorylation (OXPHOS) is a cellular process that generates most of the cellular energy required by the body. Disorders affecting OXPHOS are multisystem diseases caused by pathogenic variants in more than 50 genes. ...

Abstract	Introduction: Mitochondrial oxidative phosphorylation (OXPHOS) is a cellular process that generates most of the cellular energy required by the body. Disorders affecting OXPHOS are multisystem diseases caused by pathogenic variants in more than 50 genes. In 2017, biallelic variants in the MRPS34 gene were shown to cause combined oxidative phosphorylation deficiency type 32 (COPD32) (OMIM#617664); however, only 7 patients have been reported in the literature up to this moment. COPD32 is characterized mainly by a severe Leigh-like syndrome. Methods: Whole-exome sequencing identified a homozygous pathogenic variant in the MRPS34 gene, c.322-10G>A. Only the mother was heterozygous for this variant. SNP-array analysis was performed, which revealed a region of absence of heterozygosity in variant 16q with 9.8Mb, compatible with maternal uniparental disomy. Results/case report: We report the case of an 18-year-old female with unremarkable family history. The pregnancy was complicated by oligohydramnios, and the neonatal period was unremarkable. She evolved with low weight, mild-moderate developmental delay/intellectual disability, and hypogonadotropic hypogonadism. On examination, she had slender habitus, joint laxity, and kyphoscoliosis. The cardiac evaluation was normal, and the head MRI showed bilateral olivary nucleus degeneration that was not confirmed subsequently. Extensive metabolic studies documented only mild lactate and pyruvate elevation, and the chromosomal microarray was normal. Conclusion: We have reported the case of the first patient with COPD32 due to partial maternal uniparental disomy of chromosome 16, being first in Portugal and seventh in the literature. Contrarily to previous patients, who died in the first months of life or survived with severe DD/ID, and had a Leigh-like syndrome, this case is significantly milder, contributing to a better characterization of the phenotypic spectrum. Recurrence risk is unexpectedly low in this instance. This case illustrates the importance of segregation analysis in patients with homozygous recessive mutations.
Language	English
Publishing date	2024-01-12
Publishing country	United Arab Emirates
Document type	Journal Article
ZDB-ID	2228325-0
ISSN	2212-3873 ; 1871-5303
ISSN (online)	2212-3873
ISSN	1871-5303
DOI	10.2174/0118715303283767231120113921
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Article ; Online: Diagnostic value of p16 and Ki-67 expression in cervical glandular intraepithelial disease: A review.

Pereira Pinto, Patrícia / Zanine, Rita Maira

Annals of diagnostic pathology

2022 , Page(s) 152054

Abstract: Objectives: Cervical adenocarcinoma has been increasing in frequency, particularly among young women. Its diagnosis still presents many challenges. In addition to the difficulty in accessing the lesion, the lack of well-established criterias for ... ...

Abstract	Objectives: Cervical adenocarcinoma has been increasing in frequency, particularly among young women. Its diagnosis still presents many challenges. In addition to the difficulty in accessing the lesion, the lack of well-established criterias for cytological and histological diagnosis, and colposcopic images that are often not very clear, there are many benign situations that mimic glandular diseases. Immunohistochemistry has been used to aid this diagnosis. The purpose of this review is to evaluate the data available in the literature related to the use of p16 and Ki-67 as an auxiliary tool in the diagnosis of glandular disease. Methods: Original articles were searched in the PUBMED/MEDLINE, EMBASE, SCOPUS and BVS using different combinations of keywords and descriptors. Results: Of the 83 initial articles, 10 were selected. The criterias used for immunohistochemical interpretation showed many differences. The studies compared benign pathologies to malignant pathologies and some used panels with different immunomarkers while others used only p16. P16 showed an intense staining pattern in malignant pathologies, despite some studies have shown a negative pattern in adenocarcinoma. This can be explained by the histological subtype, not always related to HPV. Some studies have shown immunopositivity for p16 in normal tissues, tubal metaplasia and cervical endometriosis, but the staining pattern was never intense, as in malignant tissues. Ki-67 showed a more intense staining in AIS and adenocarcinoma tissues and was also present in normal tissues, mainly in tubal metaplasia and cervical endometriosis. Conclusions: Biomarkers have proved to be important tools in aiding the diagnosis of glandular lesions, either alone or in panels. Standardization in the interpretation of immunochemistry is required.
Language	English
Publishing date	2022-10-14
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	1440011-x
ISSN	1532-8198 ; 1092-9134
ISSN (online)	1532-8198
ISSN	1092-9134
DOI	10.1016/j.anndiagpath.2022.152054
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Article: Prediction of Surgical Outcome in Advanced Ovarian Cancer by Imaging and Laparoscopy: A Narrative Review.

Pinto, Patrícia / Burgetova, Andrea / Cibula, David / Haldorsen, Ingfrid S / Indrielle-Kelly, Tereza / Fischerova, Daniela

Cancers

2023 Volume 15, Issue 6

Abstract: Maximal-effort upfront or interval debulking surgery is the recommended approach for advanced-stage ovarian cancer. The role of diagnostic imaging is to provide a systematic and structured report on tumour dissemination with emphasis on key sites for ... ...

Abstract	Maximal-effort upfront or interval debulking surgery is the recommended approach for advanced-stage ovarian cancer. The role of diagnostic imaging is to provide a systematic and structured report on tumour dissemination with emphasis on key sites for resectability. Imaging methods, such as pelvic and abdominal ultrasound, contrast-enhanced computed tomography, whole-body diffusion-weighted magnetic resonance imaging and positron emission tomography, yield high diagnostic performance for diagnosing bulky disease, but they are less accurate for depicting small-volume carcinomatosis, which may lead to unnecessary explorative laparotomies. Diagnostic laparoscopy, on the other hand, may directly visualize intraperitoneal involvement but has limitations in detecting tumours beyond the gastrosplenic ligament, in the lesser sac, mesenteric root or in the retroperitoneum. Laparoscopy has its place in combination with imaging in cases where ima-ging results regarding resectability are unclear. Different imaging models predicting tumour resectability have been developed as an adjunctional objective tool. Incorporating results from tumour quantitative analyses (e.g., radiomics), preoperative biopsies and biomarkers into predictive models may allow for more precise selection of patients eligible for extensive surgery. This review will discuss the ability of imaging and laparoscopy to predict non-resectable disease in patients with advanced ovarian cancer.
Language	English
Publishing date	2023-03-22
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2527080-1
ISSN	2072-6694
ISSN	2072-6694
DOI	10.3390/cancers15061904
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Article ; Online: Hydroxychloroquine induced melanosis.

Samões, Beatriz / Abelha-Aleixo, Joana / Pinto, Patrícia / Vieira, Romana

Reumatologia clinica

2022 Volume 18, Issue 6, Page(s) 377–378

MeSH term(s)	Humans ; Hydroxychloroquine/adverse effects ; Melanosis/chemically induced
Chemical Substances	Hydroxychloroquine (4QWG6N8QKH)
Language	English
Publishing date	2022-06-06
Publishing country	Spain
Document type	Journal Article
ISSN	2173-5743
ISSN (online)	2173-5743
DOI	10.1016/j.reumae.2021.09.007
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Article ; Online: Evolutionary history and functional characterization of duplicated G protein-coupled estrogen receptors in European sea bass.

Zapater, Cinta / Moreira, Catarina / Knigge, Thomas / Monsinjon, Tiphaine / Gómez, Ana / Pinto, Patrícia I S

The Journal of steroid biochemistry and molecular biology

2023 Volume 236, Page(s) 106423

Abstract: Across vertebrates, the numerous estrogenic functions are mainly mediated by nuclear and membrane receptors, including the G protein-coupled estrogen receptor (GPER) that has been mostly associated with rapid non-genomic responses. Although Gper-mediated ...

Abstract	Across vertebrates, the numerous estrogenic functions are mainly mediated by nuclear and membrane receptors, including the G protein-coupled estrogen receptor (GPER) that has been mostly associated with rapid non-genomic responses. Although Gper-mediated signalling has been characterized in only few fish species, Gpers in fish appear to present more mechanistic functionalities as those of mammals due to additional gene duplicates. In this study, we ran a thorough investigation of the fish Gper evolutionary history in light of available genomes, we carried out the functional characterization of the two gper gene duplicates of European sea bass (Dicentrarchus labrax) using luciferase reporter gene transactivation assays, validated it with natural and synthetic estrogen agonists/antagonists and applied it to other chemicals of aquaculture and ecotoxicological interest. Phylogenetic and synteny analyses of fish gper1 and gper1-like genes suggest their duplication may have not resulted from the teleost-specific whole genome duplication. We confirmed that both sbsGper isoforms activate the cAMP signalling pathway and respond differentially to distinct estrogenic compounds. Therefore, as observed for nuclear estrogen receptors, both sbsGpers duplicates retain estrogenic activity although they differ in their specificity and potency (Gper1 being more potent and more specific than Gper1-like), suggesting a more conserved role for Gper1 than for Gper1-like. In addition, Gpers were able to respond to estrogenic environmental pollutants known to interfere with estrogen signalling, such as the phytoestrogen genistein and the anti-depressant fluoxetine, a point that can be taken into account in aquatic environment pollution screenings and chemical risk assessment, complementing previous assays for sea bass nuclear estrogen receptors.
MeSH term(s)	Animals ; Bass/genetics ; Bass/metabolism ; Phylogeny ; Estrogens/metabolism ; Receptors, Estrogen/genetics ; Receptors, Estrogen/metabolism ; Receptors, G-Protein-Coupled/genetics ; Receptors, G-Protein-Coupled/metabolism ; GTP-Binding Proteins/genetics ; GTP-Binding Proteins/metabolism ; Mammals/metabolism
Chemical Substances	Estrogens ; Receptors, Estrogen ; Receptors, G-Protein-Coupled ; GTP-Binding Proteins (EC 3.6.1.-)
Language	English
Publishing date	2023-11-07
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1049188-0
ISSN	1879-1220 ; 0960-0760
ISSN (online)	1879-1220
ISSN	0960-0760
DOI	10.1016/j.jsbmb.2023.106423
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Zs.A 708: Show issues

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Article: Understanding attitudes towards the adoption of nature-based solutions and policy priorities shaped by stakeholders’ awareness of climate change

Ferreira, Vera / Barreira, Ana Paula / Pinto, Patrícia / Panagopoulos, Thomas

Environmental science & policy. 2022 May, v. 131

2022

Abstract: Climate change is affecting cities worldwide. Accordingly, cities are required to find sustainable solutions to tackle climate change’s effects, designing bottom-up policies to enhance their success. The involvement of stakeholders plays a central role ... ...

Abstract	Climate change is affecting cities worldwide. Accordingly, cities are required to find sustainable solutions to tackle climate change’s effects, designing bottom-up policies to enhance their success. The involvement of stakeholders plays a central role in the definition of appropriate policies to tackle the challenges posed to cities by climate change. Nature-based solutions (NBS) are increasingly proposed to adapt to and mitigate the adverse effects of climate change. This study aims to assess the coherence of the policies emerging from stakeholders’ perceptions of urban climate challenges and their preferred NBS to tackle them. Indeed, it considers whether departing from different urban climate challenges, stakeholders’ choices present a coherent articulation between priority interventions, proposed solutions and expected benefits. Using a survey applied to two Portuguese cities and the chi-squared automatic interaction detector (CHAID) algorithm, we analyse the answers provided by stakeholders. The stakeholders’ perceptions and preferences were not significantly influenced by their hierarchical position in their institutions, their city’s location and socioeconomic setting. Heatwaves and temperature rise together with drought and water scarcity are identified as future urban challenges in Portugal. Priority interventions, as well as preferred NBS and their expected benefits, are addressed. The results show that stakeholders may make decisions that form a coherent policy, in which acknowledgment of climate change’s effects interconnects with related priority interventions, suitable NBS and their benefits.
Keywords	algorithms ; climate ; climate change ; drought ; environmental science ; issues and policy ; stakeholders ; surveys ; temperature ; water shortages ; Portugal
Language	English
Dates of publication	2022-05
Size	p. 149-159.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1454687-5
ISSN	1462-9011
ISSN	1462-9011
DOI	10.1016/j.envsci.2022.02.007
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Predictive value of quantitative sensory testing for acute and chronic postsurgical pain after total joint arthroplasty: a systematic review.

Paredes, Ana Cristina / Pinto, Joana Milhazes / Almeida, Armando / Pinto, Patrícia R

Pain

2021 Volume 163, Issue 3, Page(s) e385–e400

Abstract: Abstract: Quantitative sensory testing (QST) can be useful to identify high-risk patients for the development of chronic postsurgical pain. This systematic review aims to assess if presurgical sensory sensitivity measured using QST is associated with ... ...

Abstract	Abstract: Quantitative sensory testing (QST) can be useful to identify high-risk patients for the development of chronic postsurgical pain. This systematic review aims to assess if presurgical sensory sensitivity measured using QST is associated with acute and chronic postsurgical pain after total joint arthroplasty. A systematic search was performed in September 2020 in PubMed, EMBASE, Web of Science, and Scopus, using terms related to total joint arthroplasty and QST. Prospective studies were included if they reported an association between presurgical QST and postsurgical pain in adults with osteoarthritis undergoing primary unilateral total joint arthroplasty. From 2994 identified studies, 18 met the inclusion criteria (1869 patients). Total knee arthroplasty was the most common surgery (16 studies), and pressure pain threshold was the most common test (11 studies), followed by dynamic measures (9 studies). Postsurgical pain was assessed at acute (5 studies), subacute (2 studies), and chronic (13 studies) time points. Risk of bias was assessed using the Quality in Prognosis Studies tool and evaluated as low to moderate in most domains. Fourteen studies reported at least one statistically significant association between QST and pain (acute: 4 studies, subacute: 1 study, and chronic: 9 studies). Pressure pain threshold was associated with postsurgical pain in 6 studies (of 11, 55%), heat pain threshold in 2 studies (of 6, 33%), conditioned pain modulation in 1 study (of 6, 17%), and temporal summation of pain in 5 studies (of 8, 63%). The predictive role of presurgical QST for postarthroplasty pain remains unclear, mainly because of heterogeneous methodologies and inconsistent results.
MeSH term(s)	Adult ; Arthroplasty, Replacement, Knee/adverse effects ; Humans ; Pain Measurement ; Pain Threshold ; Pain, Postoperative/diagnosis ; Pain, Postoperative/etiology ; Prospective Studies
Language	English
Publishing date	2021-06-09
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Systematic Review
ZDB-ID	193153-2
ISSN	1872-6623 ; 0304-3959
ISSN (online)	1872-6623
ISSN	0304-3959
DOI	10.1097/j.pain.0000000000002385
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Zs.A 1158: Show issues

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Article ; Online: Prevalence and Interference of Chronic Pain Among People With Hemophilia: A Systematic Review and Meta-Analysis.

Paredes, Ana Cristina / Teixeira, Pedro / Almeida, Armando / Pinto, Patrícia Ribeiro

The journal of pain

2021 Volume 22, Issue 10, Page(s) 1134–1145

Abstract: Chronic pain is a common condition among people with hemophilia (PWH), associated with joint deterioration due to repeated joint bleeds. This systematic review and meta-analysis aimed to determine the prevalence of chronic pain due to haemophilia and to ... ...

Abstract	Chronic pain is a common condition among people with hemophilia (PWH), associated with joint deterioration due to repeated joint bleeds. This systematic review and meta-analysis aimed to determine the prevalence of chronic pain due to haemophilia and to analyze its interference in the lives of patients. A systematic search was performed in May and June 2019 and updated in February 2021, using PubMed, EMBASE, Web of Science and SciElo. The search included terms related to hemophilia, pain, pain prevalence and pain interference. Studies were included if they reported data referring to hemophilia-related chronic pain among adult males (age ≥18). From 3,258 identified studies, 11 met the inclusion criteria. Three studies used a proposed definition for hemophilia-related chronic pain and 8 used direct questions developed by the authors. For the global samples, prevalence ranged from 17% to 84%. The random-effects meta-analysis including all studies demonstrated a pooled prevalence of chronic pain of 46% (95% Confidence Interval, CI = 34%-58%). Subgroup analysis of samples including all disease severities or including only severe patients revealed a pooled prevalence of 48% (95% CI = 29%-67%) and 53% (95% CI = 38%-69%), respectively. High heterogeneity between studies was observed in all models. Information concerning chronic pain interference was retrieved from 1 study, reporting a mean interference of 3.7 (0-10 numerical rating scale from the Brief Pain Inventory). This systematic review revealed a wide prevalence range of hemophilia-related chronic pain across studies, varying methodologies and sample characteristics. Research in the hemophilia field should clearly distinguish between acute and chronic pain and provide complete characterization of study samples. PERSPECTIVE: Pain is a central issue in the lives of people with hemophilia, posing a significant challenge for healthcare providers. A clear picture of chronic pain due to hemophilia is precluded by high heterogeneity among studies and various definitions used to investigate its prevalence.
MeSH term(s)	Chronic Pain/epidemiology ; Chronic Pain/etiology ; Chronic Pain/physiopathology ; Chronic Pain/psychology ; Hemophilia A/complications ; Hemophilia A/epidemiology ; Humans ; Prevalence
Language	English
Publishing date	2021-05-01
Publishing country	United States
Document type	Journal Article ; Meta-Analysis ; Systematic Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2018789-0
ISSN	1528-8447 ; 1526-5900
ISSN (online)	1528-8447
ISSN	1526-5900
DOI	10.1016/j.jpain.2021.03.157
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Zs.A 5237: Show issues

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Article ; Online: Quantifying dominant bacterial genera detected in metagenomic data from fish eggs and larvae using genus-specific primers.

Najafpour, Babak / Pinto, Patricia I S / Canario, A V M / Power, Deborah M

MicrobiologyOpen

2022 Volume 11, Issue 3, Page(s) e1274

Abstract: The goal of this study was to design genus-specific primers for rapid evaluation of the most abundant bacterial genera identified using amplicon-based sequencing of the 16S rRNA gene in fish-related samples and surrounding water. Efficient genus-specific ...

Abstract	The goal of this study was to design genus-specific primers for rapid evaluation of the most abundant bacterial genera identified using amplicon-based sequencing of the 16S rRNA gene in fish-related samples and surrounding water. Efficient genus-specific primers were designed for 11 bacterial genera including Alkalimarinus, Colwellia, Enterovibrio, Marinomonas, Massilia, Oleispira, Phaeobacter, Photobacterium, Polarbacerium, Pseudomonas, and Psychrobium. The specificity of the primers was confirmed by the phylogeny of the sequenced polymerase chain reaction (PCR) amplicons that indicated primers were genus-specific except in the case of Colwellia and Phaeobacter. Copy number of the 16S rRNA gene obtained by quantitative PCR using genus-specific primers and the relative abundance obtained by 16S rRNA gene sequencing using universal primers were well correlated for the five analyzed abundant bacterial genera. Low correlations between quantitative PCR and 16S rRNA gene sequencing for Pseudomonas were explained by the higher coverage of known Pseudomonas species by the designed genus-specific primers than the universal primers used in 16S rRNA gene sequencing. The designed genus-specific primers are proposed as rapid and cost-effective tools to evaluate the most abundant bacterial genera in fish-related or potentially other metagenomics samples.
MeSH term(s)	Animals ; DNA Primers/genetics ; Fishes ; Larva ; Metagenome ; Metagenomics ; Pseudomonas/genetics ; RNA, Ribosomal, 16S/genetics ; Rhodobacteraceae
Chemical Substances	DNA Primers ; RNA, Ribosomal, 16S
Language	English
Publishing date	2022-06-28
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2661368-2
ISSN	2045-8827 ; 2045-8827
ISSN (online)	2045-8827
ISSN	2045-8827
DOI	10.1002/mbo3.1274
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