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  1. Article ; Online: Lissencephaly With Cerebellar Hypoplasia Due To a New RELN Mutation.

    Igreja, Liliana / Menezes, Catarina / Pinto, Pedro S / Freixo, João Parente / Chorão, Rui

    Pediatric neurology

    2023  Volume 149, Page(s) 137–140

    Abstract: Lissencephaly with cerebellar hypoplasia (LCH) is a rare variant form of lissencephaly, its distinctive neuroradiological phenotype being an important investigation clue regarding the potential involved genes, including variants in RELN gene. We report ... ...

    Abstract Lissencephaly with cerebellar hypoplasia (LCH) is a rare variant form of lissencephaly, its distinctive neuroradiological phenotype being an important investigation clue regarding the potential involved genes, including variants in RELN gene. We report on a case of LCH whose clinical and neuroradiological features led to the identification of a homozygous pathogenic variant in RELN gene that has not been previously reported in the scientific literature.
    MeSH term(s) Humans ; Nervous System Malformations/diagnostic imaging ; Nervous System Malformations/genetics ; Lissencephaly/diagnostic imaging ; Lissencephaly/genetics ; Homozygote ; Mutation/genetics
    Language English
    Publishing date 2023-09-25
    Publishing country United States
    Document type Case Reports ; Journal Article
    ZDB-ID 639164-3
    ISSN 1873-5150 ; 0887-8994
    ISSN (online) 1873-5150
    ISSN 0887-8994
    DOI 10.1016/j.pediatrneurol.2023.09.012
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 2260: Show issues Location:
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  2. Article ; Online: First Report of Cladosporium tenuissimum Causing Spot Diseases on Leaves and Fruits of Cucurbits in Brazil

    Gomes, Izabelle R. F. / Silva, Jarlan L. S. / Costa, Talison E. / Pinto, Pedro S. L. / da Silva, Washington L. / Ambrósio, Márcia M. Q. / Holanda, Ioná S. A.

    Plant Disease. 2023 Aug. 01, v. 107, no. 8 p.2540-

    2023  

    Abstract: Cucurbitaceae crops are widely cultivated in the northeast region of Brazil, which is the largest producer of melons and watermelons in the country (Oliveira et al. 2020). Between November and December 2020, leaves of pumpkin (Cucurbita maxima L.) and ... ...

    Abstract Cucurbitaceae crops are widely cultivated in the northeast region of Brazil, which is the largest producer of melons and watermelons in the country (Oliveira et al. 2020). Between November and December 2020, leaves of pumpkin (Cucurbita maxima L.) and watermelon (Citrullus lanatus L.) plants and leaves and fruits of melon plants (Cucumis melo L.) with moderate to severe necrotic, irregular, and brown lesions were collected from farms in the state of Rio Grande do Norte, Brazil. Fragments of diseased tissues were cut into small pieces, surface disinfested in 70% ethanol for 30 s and then in 2% sodium hypochlorite for 1 min, and washed in sterile distilled water. The disinfested pieces were plated on potato dextrose agar and incubated for 7 days in the dark at 28 ± 2°C. A total of 12 fungal isolates (four from pumpkins, one from watermelons, and seven from melons) were isolated from symptomatic leaves and fruits. All isolates in this study shared similar morphological characteristics. The colonies were dark gray to olive green in color with a velvety texture and surrounded by gray-white hyphae. The conidiophores were erect, tall, dark, and irregularly branched at the apex, containing dark conidia, with 0 to 3 septa, variable in shape and size, forming chains that were often branched, globose or subglobose, and 3 to 4.5 μm in diameter. DNA from each isolate was extracted using the SDS method (Smith et al. 2001) and subjected to PCR amplification of the internal transcribed spacer (ITS) and translation elongation factor 1-α (TEF1-α) regions with the primers ITS1/ITS4 (White et al. 1990) and EF1-728F/EF1-986R (Carbone and Kohn 1999), respectively. The amplicons were sequenced and deposited in GenBank: OP493545 to OP493556 for ITS and OP536836 to OP536847 for TEF1-α. BLASTn analysis of the ITS and TEF1-α partial sequences revealed that all 12 isolates belong to the species Cladosporium tenuissimum, with 100% nucleotide similarity with sequences of many C. tenuissimum isolates deposited in GenBank. A phylogenetic tree was constructed using the maximum parsimony method, with the concatenated sequences (ITS–TEF1-α) on MEGA X software (version 11.0.8) (Tamura et al. 2021). All 12 isolates clustered in the same clade and were closely related to the isolates A2PP5, A3I1, and XCHK2 with the respective accession numbers KU605789.1, KU605790.1, and MG873071.1 from GenBank, with 99% bootstrap support. The pathogenicity of the 12 isolates was evaluated in pumpkin and melon plants in a greenhouse. Spore suspensions (10⁶ conidia/ml) were sprayed on the leaves of healthy seedlings until runoff, only water was sprayed on control plants as the mock treatment, and five seedlings of each crop (melon and pumpkin) were inoculated in each treatment. All plants were covered with plastic bags for 2 days. Spots similar to those observed on diseased plants in the field developed on the inoculated leaves (after 7 days from the inoculation; no symptoms were observed on plants from the mock treatment), and the fungal morphology was identical to that observed on the original diseased leaves, fulfilling Kochʼs postulates. The pathogenicity test was repeated and yielded the same results. The fact that all 12 isolates were pathogenic on pumpkin and melon leaves indicates that many cucurbits are susceptible to C. tenuissimum infection. Many growers in the region are reporting similar symptoms in their melon plantations, and thus it appears that the disease incidence is becoming more severe year after year. Therefore, more research needs to be conducted to determine the epidemiology and the extension of the economic impact caused by this pathogen on cucurbits to develop strategies for disease control. To the best of our knowledge, this is the first report of C. tenuissimum causing spot diseases in cucurbits in Brazil.
    Keywords Citrullus lanatus ; Cladosporium ; Cucumis melo ; Cucurbita ; DNA ; color ; computer software ; conidia ; conidiophores ; culture media ; disease control ; disease incidence ; economic impact ; ethanol ; fungal morphology ; fungi ; greenhouses ; hyphae ; internal transcribed spacers ; pathogenicity ; pathogens ; peptide elongation factors ; phylogeny ; pumpkins ; runoff ; sodium hypochlorite ; texture ; watermelons ; Brazil ; Cladosporium tenuissimum ; cucurbits ; melon ; spot diseases
    Language English
    Dates of publication 2023-0801
    Publishing place The American Phytopathological Society
    Document type Article ; Online
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-10-22-2388-PDN
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 2043: Show issues

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  3. Article: The CT Halo Sign.

    Pinto, Pedro S

    Radiology

    2004  Volume 230, Issue 1, Page(s) 109–110

    MeSH term(s) Aspergillosis/diagnostic imaging ; Humans ; Lung Diseases, Fungal/diagnostic imaging ; Tomography, X-Ray Computed
    Language English
    Publishing date 2004-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 80324-8
    ISSN 1527-1315 ; 0033-8419
    ISSN (online) 1527-1315
    ISSN 0033-8419
    DOI 10.1148/radiol.2301020649
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  4. Article ; Online: Fetal lipomyelomeningocele: A closed neural tube defect diagnosed at second-trimester ultrasound examination.

    Sarmento-Gonçalves, Inês / Cunha, Manuela / Loureiro, Teresa / Pinto, Pedro S / Ramalho, Carla

    Journal of clinical ultrasound : JCU

    2018  Volume 47, Issue 3, Page(s) 169–171

    Abstract: Fetal lipomyelomeningocele was suspected during the second-trimester ultrasound and confirmed by magnetic resonance imaging. The pregnancy took its course and a term neonate was delivered. At 2 years of age lipomyelomeningocele surgical removal was ... ...

    Abstract Fetal lipomyelomeningocele was suspected during the second-trimester ultrasound and confirmed by magnetic resonance imaging. The pregnancy took its course and a term neonate was delivered. At 2 years of age lipomyelomeningocele surgical removal was performed. The patient is now 4 years old and, despite neurogenic bladder, is a healthy boy with normal psychomotor development for his age. This case illustrates the favorable prognosis of this entity and the importance of prompt diagnosis and multidisciplinary counseling.
    MeSH term(s) Adult ; Female ; Gestational Age ; Humans ; Infant, Newborn ; Magnetic Resonance Imaging ; Male ; Meningomyelocele/diagnosis ; Meningomyelocele/surgery ; Neural Tube Defects/diagnostic imaging ; Pregnancy ; Pregnancy Trimester, Second ; Ultrasonography, Prenatal/methods
    Language English
    Publishing date 2018-11-08
    Publishing country United States
    Document type Case Reports
    ZDB-ID 189393-2
    ISSN 1097-0096 ; 0091-2751
    ISSN (online) 1097-0096
    ISSN 0091-2751
    DOI 10.1002/jcu.22662
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1186: Show issues Location:
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  5. Article ; Online: When Decrease Aβ1-42 in CSF May Not Mean Alzheimer's Disease: Insights From Two Case Reports With Early Onset Dementia.

    Ladeira, Filipa / Cação, Gonçalo / Correia, Ana P / Pinto, Pedro S / Cavaco, Sara / Melo-Pires, Manuel / Alonso, Isabel / Taipa, Ricardo

    Alzheimer disease and associated disorders

    2018  Volume 32, Issue 4, Page(s) 359–363

    MeSH term(s) Age of Onset ; Amyloid ; Biomarkers/cerebrospinal fluid ; Dementia/diagnosis ; Dementia/pathology ; Frontotemporal Dementia/diagnosis ; Frontotemporal Dementia/pathology ; Humans ; Magnetic Resonance Imaging/methods ; Male ; Middle Aged
    Chemical Substances Amyloid ; Biomarkers
    Language English
    Publishing date 2018-03-11
    Publishing country United States
    Document type Case Reports ; Editorial
    ZDB-ID 1002700-2
    ISSN 1546-4156 ; 0893-0341
    ISSN (online) 1546-4156
    ISSN 0893-0341
    DOI 10.1097/WAD.0000000000000248
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    Zs.A 2569: Show issues Location:
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  6. Article ; Online: Birth-related injury to the head and cervical spine in neonates.

    Tekes, Aylin / Pinto, Pedro S / Huisman, Thierry A G M

    Magnetic resonance imaging clinics of North America

    2011  Volume 19, Issue 4, Page(s) 777–90; viii

    Abstract: Birth-related injury is defined as any traumatic or ischemic event sustained during the process of delivery. Perinatally acquired disease processes secondary to birth-related injury can be traumatic or ischemic in nature. In this article, the authors ... ...

    Abstract Birth-related injury is defined as any traumatic or ischemic event sustained during the process of delivery. Perinatally acquired disease processes secondary to birth-related injury can be traumatic or ischemic in nature. In this article, the authors focus on traumatic/mechanical injuries. Other diseases of the perinatal time period, including germinal matrix hemorrhages and hypoxic-ischemic encephalopathy, are beyond the objective of this review.
    MeSH term(s) Birth Injuries/diagnosis ; Brain/pathology ; Craniocerebral Trauma/diagnosis ; Female ; Humans ; Image Enhancement/methods ; Infant, Newborn ; Magnetic Resonance Imaging/methods ; Male ; Neuroimaging/methods ; Postnatal Care/methods ; Spinal Injuries/diagnosis ; Spine/pathology
    Language English
    Publishing date 2011-11
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 1196851-5
    ISSN 1557-9786 ; 1064-9689
    ISSN (online) 1557-9786
    ISSN 1064-9689
    DOI 10.1016/j.mric.2011.08.004
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    Se 1375: Show issues Location:
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  7. Article ; Online: Adult-onset dystonia in Aicardi-Goutières syndrome.

    Taipa, Ricardo / Pinto, Pedro S / Magalhães, Marina

    Movement disorders : official journal of the Movement Disorder Society

    2010  Volume 25, Issue 6, Page(s) 791–793

    MeSH term(s) Abnormalities, Multiple ; Adult ; Autoimmune Diseases of the Nervous System/complications ; Autoimmune Diseases of the Nervous System/genetics ; Dystonic Disorders/diagnosis ; Dystonic Disorders/etiology ; Female ; Humans ; Magnetic Resonance Imaging ; Video Recording
    Language English
    Publishing date 2010-04-30
    Publishing country United States
    Document type Case Reports ; Letter
    ZDB-ID 607633-6
    ISSN 1531-8257 ; 0885-3185
    ISSN (online) 1531-8257
    ISSN 0885-3185
    DOI 10.1002/mds.23013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.MO 238: Show issues

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  8. Article ; Online: Non-invasive imaging of intracranial pediatric vascular lesions.

    Huisman, Thierry A G M / Singhi, Samata / Pinto, Pedro S

    Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery

    2010  Volume 26, Issue 10, Page(s) 1275–1295

    Abstract: Objectives: The goal of this review is to discuss the different non-invasive imaging techniques as well as the age-specific pediatric vascular pathologies and their imaging features.: Material and methods: Ultrasound, computed tomography, and ... ...

    Abstract Objectives: The goal of this review is to discuss the different non-invasive imaging techniques as well as the age-specific pediatric vascular pathologies and their imaging features.
    Material and methods: Ultrasound, computed tomography, and magnetic resonance imaging features of pediatric ischemic stroke, intracranial hemorrhage, aneurysms, cavernomas, developmental venous anomalies, and arteriovenous malformations are presented. In addition, multiple non-invasive angiographic techniques (CT and MR angiography) and functional MRI sequences (diffusion, perfusion, and susceptibility-weighted imaging) are discussed.
    Conclusion: Neurovascular imaging plays a central role in the early, sensitive, and specific diagnosis of pediatric intracranial vascular disorders. A detailed knowledge of the quality and exact angioarchitecture of pediatric vascular pathologies as well as their impact on the cerebral hemo-dynamics is essential to guide and monitor treatment options and to predict functional outcome.
    MeSH term(s) Brain Ischemia/diagnosis ; Central Nervous System Venous Angioma/diagnosis ; Cerebrovascular Disorders/diagnosis ; Cerebrovascular Disorders/diagnostic imaging ; Cerebrovascular Disorders/pathology ; Child ; Diagnostic Imaging/methods ; Diagnostic Imaging/trends ; Humans ; Intracranial Aneurysm/diagnosis ; Intracranial Arteriovenous Malformations/diagnosis ; Intracranial Hemorrhages/diagnosis ; Magnetic Resonance Angiography ; Magnetic Resonance Imaging ; Stroke/diagnosis ; Tomography, X-Ray Computed ; Ultrasonography
    Language English
    Publishing date 2010-07-02
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 605988-0
    ISSN 1433-0350 ; 0302-2803 ; 0256-7040
    ISSN (online) 1433-0350
    ISSN 0302-2803 ; 0256-7040
    DOI 10.1007/s00381-010-1203-1
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    Zs.A 2036: Show issues Location:
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  9. Book ; Conference proceedings: Earthquake geotechnical engineering

    Sêco e Pinto, Pedro S

    : proceedings of the Second International Conference on Earthquake Geotechnical Engineering, Lisboa, Portugal, 21 - 25 June 1999

    1999  

    Institution International Conference on Earthquake Geotechnical Engineering
    Event/congress International Conference on Earthquake Geotechnical Engineering (2, 1999.06.21-25, Lisboa) ; SICEGE (2, 1999.06.21-25, Lisboa)
    Author's details ed. by Pedro S. Seco e Pinto
    Language English
    Size XIV, 462 S, graph. Darst
    Publisher Balkema
    Publishing place Rotterdam u.a.
    Document type Book ; Conference proceedings
    ISBN 9058091171 ; 9789058091178
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  10. Book ; Conference proceedings: Earthquake geotechnical engineering

    Sêco e Pinto, Pedro S

    : proceedings of the Second International Conference on Earthquake Geotechnical Engineering, Lisboa, Portugal, 21 - 25 June 1999

    1999  

    Institution International Conference on Earthquake Geotechnical Engineering
    Event/congress International Conference on Earthquake Geotechnical Engineering (2, 1999.06.21-25, Lisboa) ; SICEGE (2, 1999.06.21-25, Lisboa)
    Author's details ed. by Pedro S. Seco e Pinto
    Language English
    Size VIII S., S. 465 - 792, graph. Darst
    Publisher Balkema
    Publishing place Rotterdam u.a.
    Document type Book ; Conference proceedings
    ISBN 905809118X ; 9789058091185
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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