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  1. Article: Gold Nanoparticles Inhibit Steroid-Insensitive Asthma in Mice Preserving Histone Deacetylase 2 and NRF2 Pathways.

    Serra, Magda F / Cotias, Amanda C / Pimentel, Andreza S / Arantes, Ana Carolina S de / Pires, Ana Lucia A / Lanzetti, Manuella / Hickmann, Jandir M / Barreto, Emiliano / Carvalho, Vinicius F / Silva, Patrícia M R E / Cordeiro, Renato S B / Martins, Marco Aurélio

    Antioxidants (Basel, Switzerland)

    2022  Volume 11, Issue 9

    Abstract: Background: Gold nanoparticles (AuNPs) can inhibit pivotal pathological changes in experimental asthma, but their effect on steroid-insensitive asthma is unclear. The current study assessed the effectiveness of nebulized AuNPs in a murine model of ... ...

    Abstract Background: Gold nanoparticles (AuNPs) can inhibit pivotal pathological changes in experimental asthma, but their effect on steroid-insensitive asthma is unclear. The current study assessed the effectiveness of nebulized AuNPs in a murine model of glucocorticoid (GC)-resistant asthma.
    Methods: A/J mice were sensitized and subjected to intranasal instillations of ovalbumin (OVA) once a week for nine weeks. Two weeks after starting allergen stimulations, mice were subjected to Budesonide or AuNP nebulization 1 h before stimuli. Analyses were carried out 24 h after the last provocation.
    Results: We found that mice challenged with OVA had airway hyperreactivity, eosinophil, and neutrophil infiltrates in the lung, concomitantly with peribronchiolar fibrosis, mucus production, and pro-inflammatory cytokine generation compared to sham-challenged mice. These changes were inhibited in mice treated with AuNPs, but not Budesonide. In the GC-resistant asthmatic mice, oxidative stress was established, marked by a reduction in nuclear factor erythroid 2-related factor 2 (NRF2) levels and catalase activity, accompanied by elevated values of thiobarbituric acid reactive substances (TBARS), phosphoinositide 3-kinases δ (PI3Kδ) expression, as well as a reduction in the nuclear expression of histone deacetylase 2 (HDAC2) in the lung tissue, all of which sensitive to AuNPs but not Budesonide treatment.
    Conclusion: These findings suggest that AuNPs can improve GC-insensitive asthma by preserving HDAC2 and NRF2.
    Language English
    Publishing date 2022-08-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704216-9
    ISSN 2076-3921
    ISSN 2076-3921
    DOI 10.3390/antiox11091659
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Gold Nanoparticles Inhibit Steroid-Insensitive Asthma in Mice Preserving Histone Deacetylase 2 and NRF2 Pathways

    Serra, Magda F. / Cotias, Amanda C. / Pimentel, Andreza S. / Arantes, Ana Carolina S. de / Pires, Ana Lucia A. / Lanzetti, Manuella / Hickmann, Jandir M. / Barreto, Emiliano / Carvalho, Vinicius F. / Silva, Patrícia M. R. e / Cordeiro, Renato S. B. / Martins, Marco Aurélio

    Antioxidants. 2022 Aug. 26, v. 11, no. 9

    2022  

    Abstract: Background: Gold nanoparticles (AuNPs) can inhibit pivotal pathological changes in experimental asthma, but their effect on steroid-insensitive asthma is unclear. The current study assessed the effectiveness of nebulized AuNPs in a murine model of ... ...

    Abstract Background: Gold nanoparticles (AuNPs) can inhibit pivotal pathological changes in experimental asthma, but their effect on steroid-insensitive asthma is unclear. The current study assessed the effectiveness of nebulized AuNPs in a murine model of glucocorticoid (GC)-resistant asthma. Methods: A/J mice were sensitized and subjected to intranasal instillations of ovalbumin (OVA) once a week for nine weeks. Two weeks after starting allergen stimulations, mice were subjected to Budesonide or AuNP nebulization 1 h before stimuli. Analyses were carried out 24 h after the last provocation. Results: We found that mice challenged with OVA had airway hyperreactivity, eosinophil, and neutrophil infiltrates in the lung, concomitantly with peribronchiolar fibrosis, mucus production, and pro-inflammatory cytokine generation compared to sham-challenged mice. These changes were inhibited in mice treated with AuNPs, but not Budesonide. In the GC-resistant asthmatic mice, oxidative stress was established, marked by a reduction in nuclear factor erythroid 2-related factor 2 (NRF2) levels and catalase activity, accompanied by elevated values of thiobarbituric acid reactive substances (TBARS), phosphoinositide 3-kinases δ (PI3Kδ) expression, as well as a reduction in the nuclear expression of histone deacetylase 2 (HDAC2) in the lung tissue, all of which sensitive to AuNPs but not Budesonide treatment. Conclusion: These findings suggest that AuNPs can improve GC-insensitive asthma by preserving HDAC2 and NRF2.
    Keywords allergens ; animal models ; asthma ; atomization ; catalase ; cytokines ; fibrosis ; glucocorticoids ; histone deacetylase ; lungs ; mucus ; nanogold ; neutrophils ; ovalbumin ; oxidative stress
    Language English
    Dates of publication 2022-0826
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2704216-9
    ISSN 2076-3921
    ISSN 2076-3921
    DOI 10.3390/antiox11091659
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: 15-Deoxy-Delta-12,14-Prostaglandin J

    Coutinho, Diego S / Anjos-Valotta, Edna A / do Nascimento, Caio V M F / Pires, Ana Lucia A / Napimoga, Marcelo H / Carvalho, Vinícius F / Torres, Rafael C / E Silva, Patrícia M R / Martins, Marco A

    Frontiers in immunology

    2017  Volume 8, Page(s) 740

    Abstract: 15-deoxy-Δ-12,14-prostaglandin ... ...

    Abstract 15-deoxy-Δ-12,14-prostaglandin J
    Language English
    Publishing date 2017
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606827-8
    ISSN 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2017.00740
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Effect of selective phosphodiesterase inhibitors on the rat eosinophil chemotactic response in vitro

    Alves Alessandra C / Pires Ana Lucia A / Lagente Vincent / Cordeiro Renato SB / Martins Marco A / Silva Patricia MR e

    Memórias do Instituto Oswaldo Cruz., Vol 92, Iss suppl.2, Pp 201-

    1997  Volume 204

    Abstract: In the present study, we have performed a comparative analysis of the effect of selective inhibitors of phosphodiesterase (PDE) type III, IV and V on eosinophil chemotaxis triggered by platelet activating factor (PAF) and leukotriene B4 (LTB4) in vitro. ... ...

    Abstract In the present study, we have performed a comparative analysis of the effect of selective inhibitors of phosphodiesterase (PDE) type III, IV and V on eosinophil chemotaxis triggered by platelet activating factor (PAF) and leukotriene B4 (LTB4) in vitro. The effect of the analogues N6-2'-O-dibutyryladenosine 3':5' cyclic monophosphate (Bt2 cyclic AMP) and N2-2'-O- dibutyrylguanosine 3':5' cyclic monophosphate (Bt2 cyclic GMP) has also been determined. The eosinophils were obtained from the peritoneal cavity of naive Wistar rats and purified in discontinuous Percoll gradients to 85-95% purity. We observed that pre-incubation of eosinophils with the PDE type IV inhibitor rolipram suppressed the chemotactic response triggered by PAF and LTB4, in association with an increase in the intracellular levels of cyclic AMP. In contrast, neither zaprinast (type V inhibitor) nor type III inhibitors milrinone and SK&F 94836 affected the eosinophil migration. Only at the highest concentration tested did the analogue Bt2 cyclic AMP suppress the eosinophil chemotaxis, under conditions where Bt2 cyclic GMP was ineffective. We have concluded that inhibition of PDE IV, but not PDE III or V, was able to block the eosinophil chemotaxis in vitro, suggesting that the suppressive activity of selective PDE IV inhibitors on tissue eosinophil accumulation may, at least, be partially dependent on their ability to directly inhibit the eosinophil migration.
    Keywords eosinophil ; migration ; phosphodiesterase inhibitors ; Arctic medicine. Tropical medicine ; RC955-962 ; Microbiology ; QR1-502
    Subject code 500
    Language English
    Publishing date 1997-01-01T00:00:00Z
    Publisher Instituto Oswaldo Cruz, Ministério da Saúde
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: Contribution of mast cells and snake venom metalloproteinases to the hyperalgesia induced by Bothrops jararaca venom in rats.

    Bonavita, André Gustavo C / da Costa, Aline S / Pires, Ana Lucia A / Neves-Ferreira, Ana G C / Perales, Jonas / Cordeiro, Renato S B / Martins, Marco A / e Silva, Patrícia M R

    Toxicon : official journal of the International Society on Toxinology

    2006  Volume 47, Issue 8, Page(s) 885–893

    Abstract: Bothrops jararaca venom (Bjv) is known to induce local inflammation and severe pain. Since, mast cells are able to secrete mediators involved in algesic processes, in this study we examined the putative role of these cells in the hyperalgesia triggered ... ...

    Abstract Bothrops jararaca venom (Bjv) is known to induce local inflammation and severe pain. Since, mast cells are able to secrete mediators involved in algesic processes, in this study we examined the putative role of these cells in the hyperalgesia triggered by Bjv in the rat paw. We noted that treatment with mast cell stabilizer sodium cromoglicate as well as with histamine and 5-hydroxytriptamine receptor antagonists meclizine and methysergide, respectively, inhibited the Bjv-induced hyperalgesia. In addition, we showed that stimulation of isolated rat peritoneal mast cells with Bjv in vitro resulted in the release of stored and neo-generated inflammatory mediators such as histamine and leukotriene C(4), respectively. Bjv-induced histamine secretion was clearly sensitive to treatment with sodium cromoglicate and sodium nedocromil. We further observed that metalloproteinase inhibitors 1,10-phenantroline and DM43 inhibited mast cell degranulation in vitro, under conditions where inhibitors of phospholipase A(2) as well as of serine- and cysteine-proteinases were inactive. Altogether, our findings indicate that mast cells seem to contribute to the hyperalgesia caused by Bjv in the rat paw, and also provide evidence that this response might be dependent on the ability of the Bjv to activate directly mast cells.
    MeSH term(s) Animals ; Bothrops ; Crotalid Venoms/toxicity ; Female ; Hyperalgesia/chemically induced ; Male ; Mast Cells/drug effects ; Mast Cells/physiology ; Metalloproteases/toxicity ; Rats ; Rats, Wistar ; Time Factors
    Chemical Substances Crotalid Venoms ; Metalloproteases (EC 3.4.-)
    Language English
    Publishing date 2006-06-15
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 204479-1
    ISSN 1879-3150 ; 0041-0101
    ISSN (online) 1879-3150
    ISSN 0041-0101
    DOI 10.1016/j.toxicon.2006.02.017
    Database MEDical Literature Analysis and Retrieval System OnLINE

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