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Article: Biochemical Properties and Immunogenic Epitopes of

Khazaei, Sasan / Dalimi, Abdolhossein / Pirestani, Majid / Ghafarifar, Fatemeh

2024 Volume 19, Issue 1, Page(s) 61–74

Abstract: Background: The current : Methods: Several web tools were employed to predict physico-chemical properties, antigenicity, allergenicity, solubility, post-translational modification (PTM) sites, subcellular localization, signal peptide, transmembrane ... ...

Abstract	Background: The current Methods: Several web tools were employed to predict physico-chemical properties, antigenicity, allergenicity, solubility, post-translational modification (PTM) sites, subcellular localization, signal peptide, transmembrane domain, secondary and tertiary structure followed by refinement and validations. In addition, B-cell epitopes were predicted and were screened using various web servers, while MHC-binding and CTL epitopes were predicted using IEDB and NetCTL servers, respectively. Results: The protein had 219 residues with a molecular weight of 25.55 kDa and alkaline isoelectric pH (7.5). This protein was stable, thermo-tolerant (aliphatic index: 78.04) and hydrophilic (GRAVY: -0.440). The predicted antigenicity scores were low and the protein was nonallergenic in nature. There were no transmembrane domain and signal peptide in the sequence. Moreover, several B-cell, MHC-binding and CTL epitopes were found in the EgGST protein, which could be further used in multi-epitope vaccines. Conclusion: Further studies are needed on the development of vaccines in vivo using EgGST alone or in combination with other antigens in the future.
Language	English
Publishing date	2024-04-23
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2388453-8
ISSN	2008-238X ; 1735-7020
ISSN (online)	2008-238X
ISSN	1735-7020
DOI	10.18502/ijpa.v19i1.15205
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Article: Microscopic and Molecular Detection of

Dalir Ghaffari, Ali / Dalimi, Abdolhossein / Pirestani, Majid

Iranian journal of parasitology

2023 Volume 17, Issue 4, Page(s) 535–542

Abstract: Background: Sarcocystis: Methods: Totally, 100 bovine heart samples were collected from the main slaughterhouse of Shahriar, Meysam slaughterhouse, west of Tehran in 2016. At first, heart samples were completely examined for the presence of ... ...

Abstract	Background: Sarcocystis Methods: Totally, 100 bovine heart samples were collected from the main slaughterhouse of Shahriar, Meysam slaughterhouse, west of Tehran in 2016. At first, heart samples were completely examined for the presence of sarcocystic macrocysts. Then, for microscopic examination, 50 g of each heart was digested in sterile condition using pepsin acid digestion method. Then, the species of the parasite were detected by PCR-RFLP technique and sequencing. Results: Overall, 97 of 100 of the heart muscle samples were infected with Conclusion: S. cruzi
Language	English
Publishing date	2023-01-24
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2388453-8
ISSN	2008-238X ; 1735-7020
ISSN (online)	2008-238X
ISSN	1735-7020
DOI	10.18502/ijpa.v17i4.11281
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Article: The Role of Some Free-Ranging Animals in the Transmission of Multi-Host Species of

Mirzaghavami, Mehran / Sadraei, Javid / Pirestani, Majid / Bahadory, Saeed

Iranian journal of parasitology

2023 Volume 18, Issue 3, Page(s) 313–323

Abstract: Background: We aimed to characterize : Methods: Fifty-five animal origin : Results: By the molecular method, : Conclusion: Free-ranging animals are infected by species/subtype ... ...

Abstract	Background: We aimed to characterize Methods: Fifty-five animal origin Results: By the molecular method, Conclusion: Free-ranging animals are infected by species/subtype of
Language	English
Publishing date	2023-10-23
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2388453-8
ISSN	2008-238X ; 1735-7020
ISSN (online)	2008-238X
ISSN	1735-7020
DOI	10.18502/ijpa.v18i3.13754
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Article ; Online: Assessment of tissue levels of miR-146a and proinflammatory cytokines in experimental cerebral toxoplasmosis following atovaquone and clindamycin treatment: An in vivo study.

Zouei, Nima / Dalimi, Abdolhossein / Pirestani, Majid / Ghaffarifar, Fatemeh

Microbial pathogenesis

2023 Volume 184, Page(s) 106340

Abstract: Background: Despite recent advances for treating cerebral toxoplasmosis (CT), monitoring the parasite burden and treatment response is still challenging. miRNAs are small non-coding RNAs with regulatory functions that can be used in diagnosis and ... ...

Abstract	Background: Despite recent advances for treating cerebral toxoplasmosis (CT), monitoring the parasite burden and treatment response is still challenging. miRNAs are small non-coding RNAs with regulatory functions that can be used in diagnosis and treatment monitoring. We investigated the changes in miR-146a, BAG-1 gene, IL-6, and IL-10 tissue levels in the brain of BALB/c mice with chronic CT caused by the PRU strain of T. gondii following anti-parasitic and antibiotic treatment. Method: Fifty-three 6-to 8-week-old BALB/c mice were infected using intraperitoneal inoculation of cerebral cysts of T. gondii PRU strain and then divided into five groups as follows: group 1 included mice treated with 100 mg/kg/d Atovaquone (AT), group 2 included mice treated with 400 mg/kg/d clindamycin (CL), group 3 included mice treated with combination therapy (AT + CL), group 4 included infected untreated mice as a positive control (PC), and; group 5 included uninfected untreated mice as negative control (NC). After the completion of the treatment course, tissue level of mir-146a, miR-155, BAG-1 gene, IL-6, and IL-10 was investigated with real-time polymerase chain reaction. The IL-6/IL-10 ratio was calculated as an indicator of immune response. Moreover, brain cyst numbers were counted on autopsy samples. Results: miR-146a, IL-6, IL-10, and BAG-1 genes were expressed in PC, but not in the NC group; miR-146a, IL-6, IL-10, and BAG-1 gene expression were significantly lower in AT, CL, and AT + CL compared with PC. MiR-146a and BAG-1 levels in AT and CL were not different statistically, however, they both had lower levels compared to AT + CL (P < 0.01). There was no difference in the expression of IL-6 and IL-10 between treatment groups. BAG-1 expression was significantly lower in AT, than in CL and AT + CL (P < 0.0089 and < 0.002, respectively). The PC group showed a higher ratio of IL-6/IL-10, although this increase was not statistically significant. It is noteworthy that the treatment with AT reduced this ratio; in the inter-group comparison, this ratio showed a decrease in the AT and AT + CL compared to the PC. The number of brain tissue cysts was significantly lower in AT, CL, and AT + CL, than in PC (p < 0.0001). AT had significantly lower brain cysts than CL and AT + CL (P < 0.0001). Conclusion: It seems that the factors studied in the current research (microRNA and cytokines) are a suitable index for evaluating the response to antiparasitic and antibiotic treatment. However, more studies should be conducted in the future to confirm our findings.
MeSH term(s)	Animals ; Mice ; Toxoplasmosis, Cerebral/drug therapy ; Atovaquone/pharmacology ; Atovaquone/therapeutic use ; Cytokines/metabolism ; Clindamycin/pharmacology ; Clindamycin/therapeutic use ; Interleukin-10/genetics ; Interleukin-6 ; Toxoplasma/metabolism ; MicroRNAs/genetics ; Cysts ; Anti-Bacterial Agents
Chemical Substances	Atovaquone (Y883P1Z2LT) ; Cytokines ; Clindamycin (3U02EL437C) ; Interleukin-10 (130068-27-8) ; Interleukin-6 ; MicroRNAs ; Anti-Bacterial Agents
Language	English
Publishing date	2023-09-06
Publishing country	England
Document type	Journal Article
ZDB-ID	632772-2
ISSN	1096-1208 ; 0882-4010
ISSN (online)	1096-1208
ISSN	0882-4010
DOI	10.1016/j.micpath.2023.106340
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Article ; Online: Molecular characterization and genotyping of Toxoplasma gondii in free-living animals in Iran: Effect of One Health

Esmaeilifallah, Mahsa / Sadraei, Javid / Pirestani, Majid / Kalantari, Reza

Veterinary Parasitology: Regional Studies and Reports. 2022 Nov., v. 36, p. 100808

2022 , Page(s) 100808

Abstract: To understand the transmission of Toxoplasma gondii, this parasite's genetic diversity distribution in free-living hosts is essential. This research's objective is the molecular genotyping of T. gondii isolates from the brain and muscles of Columbidae, ... ...

Abstract	To understand the transmission of Toxoplasma gondii, this parasite's genetic diversity distribution in free-living hosts is essential. This research's objective is the molecular genotyping of T. gondii isolates from the brain and muscles of Columbidae, Corvidae, Rattus, and Felidae of Mianeh County, East-Azerbaijan Province, Northwest Iran. Three hundred fifty samples were taken. For the genotyping of T. gondii, the GRA6 gene was amplified and digested by the Tru1I (MseI) enzyme. Results of RFLP were confirmed by sequencing and phylogenetic analysis. In total, 52%, 34%, 24%, and 50% of Columbidae, Corvidae, Rattus, and Felidae were positive for T. gondii DNA, respectively. All isolated Columbidae were identified as genotype III (100%). Also, 94.1% and 5.9% of Corvidae isolates, 84.4% and 15.6% of the Rattus isolates, and 51.7% and 48.3% of the Felidae isolates belonged to genotypes III and II, respectively. This study is the first to evaluate genetic similarity and phylogenetic analysis between many definitive and intermediated hosts in northwestern Iran. The finding indicates that the T. gondii cycle is maintained among these hosts. As a result, their presence in the environment can be a risk factor for transmitting the infection to humans. Due to demographic and geographic differences in various regions, further studies are required to determine the genetic population structure.
Keywords	Columbidae ; Corvidae ; DNA ; Felidae ; Rattus ; Toxoplasma gondii ; brain ; enzymes ; genes ; genetic similarity ; genetic variation ; genotype ; genotyping ; parasites ; phylogeny ; population structure ; risk factors ; veterinary parasitology ; Iran ; One Health ; PCR-RFLP ; Toxoplasmosis
Language	English
Dates of publication	2022-11
Size	p. 100808
Publishing place	Elsevier B.V.
Document type	Article ; Online
ISSN	2405-9390
DOI	10.1016/j.vprsr.2022.100808
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Article: The Frequency of Intestinal Parasitic Infections in COVID-19 Patients: A Case-Control Study in Tehran, Capital of Iran.

Taghipour, Ali / Pirestani, Majid / Hamidi Farahani, Ramin / Barati, Mohammad

Journal of tropical medicine

2023 Volume 2023, Page(s) 5359823

Abstract: The present study was done to evaluate the prevalence of intestinal parasitic infections (IPIs) in patients with COVID-19 in health care centers (Imam Reza and Golestan hospitals), Tehran, capital of Iran. By designing a matched case-control study, 200 ... ...

Abstract	The present study was done to evaluate the prevalence of intestinal parasitic infections (IPIs) in patients with COVID-19 in health care centers (Imam Reza and Golestan hospitals), Tehran, capital of Iran. By designing a matched case-control study, 200 fecal samples were collected for each of the COVID-19 patients and healthy individuals. Nasopharyngeal/oropharyngeal swab samples were collected from all participants for the diagnosis of COVID-19. RNA extraction was performed, and then real time reverse-transcription polymerase chain reaction (rRT-PCR) assay was applied to detect viral RNA. Considering the lung complications, 25%> lung complications was detected in 49 patients, 25-49% in 42 patients, and 50%≤ in 109 patients. Fecal samples were examined using different parasitological techniques. After nested-PCR, sequencing was applied to identify
Language	English
Publishing date	2023-09-08
Publishing country	Egypt
Document type	Journal Article
ZDB-ID	2546526-0
ISSN	1687-9694 ; 1687-9686
ISSN (online)	1687-9694
ISSN	1687-9686
DOI	10.1155/2023/5359823
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Article: Molecular Detection of

Azarm, Amrollah / Dalimi, Abdolhossein / Pirestani, Majid / Mohammadiha, Anita

Iranian journal of parasitology

2022 Volume 17, Issue 2, Page(s) 159–166

Abstract: Background: Fleas (Insecta: Siphonaptera) are considered as highly specialized bloodsucking on mammals such as humans, livestock, dog, cat, rabbit, squirrels, rats, and mice. The desire for blood feeding from warm-blooded animals has led to becoming an ... ...

Abstract	Background: Fleas (Insecta: Siphonaptera) are considered as highly specialized bloodsucking on mammals such as humans, livestock, dog, cat, rabbit, squirrels, rats, and mice. The desire for blood feeding from warm-blooded animals has led to becoming an intermediate host for some tapeworms like Methods: Fleas were collected from 42 dogs using brushing the hair in Meshkinshahr for one year (2014-2015). After the morphological study, fleas were preserved in 90% ethanol for molecular identification. After DNA extraction, the 28S ribosomal RNA gene (∼670 bp) of Results: Overs, 974 fleas were collected from the dogs. In the morphological study, three species Conclusion: Ct. canis
Language	English
Publishing date	2022-08-10
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2388453-8
ISSN	2008-238X ; 1735-7020
ISSN (online)	2008-238X
ISSN	1735-7020
DOI	10.18502/ijpa.v17i2.9531
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Article ; Online: Molecular characterization and genotyping of Toxoplasma gondii in free-living animals in Iran: Effect of One Health.

Esmaeilifallah, Mahsa / Sadraei, Javid / Pirestani, Majid / Kalantari, Reza

Veterinary parasitology, regional studies and reports

2022 Volume 36, Page(s) 100808

Abstract	To understand the transmission of Toxoplasma gondii, this parasite's genetic diversity distribution in free-living hosts is essential. This research's objective is the molecular genotyping of T. gondii isolates from the brain and muscles of Columbidae, Corvidae, Rattus, and Felidae of Mianeh County, East-Azerbaijan Province, Northwest Iran. Three hundred fifty samples were taken. For the genotyping of T. gondii, the GRA6 gene was amplified and digested by the Tru1I (MseI) enzyme. Results of RFLP were confirmed by sequencing and phylogenetic analysis. In total, 52%, 34%, 24%, and 50% of Columbidae, Corvidae, Rattus, and Felidae were positive for T. gondii DNA, respectively. All isolated Columbidae were identified as genotype III (100%). Also, 94.1% and 5.9% of Corvidae isolates, 84.4% and 15.6% of the Rattus isolates, and 51.7% and 48.3% of the Felidae isolates belonged to genotypes III and II, respectively. This study is the first to evaluate genetic similarity and phylogenetic analysis between many definitive and intermediated hosts in northwestern Iran. The finding indicates that the T. gondii cycle is maintained among these hosts. As a result, their presence in the environment can be a risk factor for transmitting the infection to humans. Due to demographic and geographic differences in various regions, further studies are required to determine the genetic population structure.
MeSH term(s)	Humans ; Animals ; Rats ; One Health ; Toxoplasma/genetics ; Genotype ; Iran/epidemiology ; Phylogeny ; Columbidae ; Felidae
Language	English
Publishing date	2022-11-11
Publishing country	Netherlands
Document type	Case Reports
ISSN	2405-9390
ISSN (online)	2405-9390
DOI	10.1016/j.vprsr.2022.100808
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Article: Multi-epitope vaccine expressed in Leishmania tarentolae confers protective immunity to Toxoplasma gondii in BALB/c mice

Majidiani, Hamidreza / Dalimi, Abdolhossein / Ghaffarifar, Fatemeh / Pirestani, Majid

Microbial pathogenesis. 2021 June, v. 155

2021

Abstract: Current study deals with a novel multi-epitope vaccine designed in silico and its confirmation experiments for potential efficacy in BALB/c mice. Major histocompatibility complex (MHC)-binding and B-cell binding epitopes of five Toxoplasma antigens (SAG1, ...

Abstract	Current study deals with a novel multi-epitope vaccine designed in silico and its confirmation experiments for potential efficacy in BALB/c mice. Major histocompatibility complex (MHC)-binding and B-cell binding epitopes of five Toxoplasma antigens (SAG1, ROP16, GRA12, MIC4 and M2AP) were predicted. Selected epitopes were fused together using SAPGTP linker, and antigenicity, allergenicity, physico-chemical features, secondary and tertiary structures and validations were all performed via bioinformatics servers. Then, vaccine construct was cloned into pLEXSY-neo 2.1 vector. After Leishmania tarentolae transfection, live recombinant and wild parasites were subcutaneously injected into 6–8 week female BALB/c mice and immune responses were measured. Results showed that the peptide possessed 282 amino acid residues with average molecular weight of 28.06 kDa. About 90% of the peptide residues were incorporated in favored and allowed regions of the Ramachandran plot. Vaccinated mice showed remarkably elevated levels of specific antibodies (P < 0.05) with predominance of IgG2a production. Also, a Th₁ immune response with production of IFN-γ and relatively increased survival rate against intraperitoneal challenge with RH strain was demonstrated in immunized mice than control groups (P < 0.05). Also, very low levels of IL-4 were demonstrated, which showed statistically significant association with controls (P < 0.05). The findings clarified that multi-epitope vaccine expressed in Leishmania tarentolae induced significant immune responses against acute toxoplasmosis.
Keywords	B-lymphocytes ; Leishmania tarentolae ; Toxoplasma gondii ; allergenicity ; amino acids ; bioinformatics ; computer simulation ; epitopes ; females ; immune response ; immunoglobulin G ; interleukin-4 ; major histocompatibility complex ; molecular weight ; pathogenesis ; survival rate ; toxoplasmosis ; transfection ; vaccines
Language	English
Dates of publication	2021-06
Publishing place	Elsevier Ltd
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	632772-2
ISSN	1096-1208 ; 0882-4010
ISSN (online)	1096-1208
ISSN	0882-4010
DOI	10.1016/j.micpath.2021.104925
Database	NAL-Catalogue (AGRICOLA)

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Article: In vitro

Bahadory, Saeed / Sadraei, Javid / Zibaei, Mohammad / Pirestani, Majid / Dalimi, Abdolhossein

Frontiers in pharmacology

2022 Volume 13, Page(s) 878724

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2022-09-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2022.878724
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