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  1. Article ; Online: Industrial Production of Proteins with

    Barone, Giovanni Davide / Emmerstorfer-Augustin, Anita / Biundo, Antonino / Pisano, Isabella / Coccetti, Paola / Mapelli, Valeria / Camattari, Andrea

    Biomolecules

    2023  Volume 13, Issue 3

    Abstract: Since the mid-1960s, methylotrophic ... ...

    Abstract Since the mid-1960s, methylotrophic yeast
    MeSH term(s) Humans ; Pichia/genetics ; Pichia/metabolism ; Saccharomycetales ; Yeasts ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism
    Chemical Substances Recombinant Proteins
    Language English
    Publishing date 2023-02-26
    Publishing country Switzerland
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom13030441
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: In Vitro

    De Leonardis, Francesco / Barile, Simona Nicole / Cianci, Claudia / Pisano, Isabella / Merla, Giuseppe / Pappalettera, Giovanni / Casavola, Caterina / Pappalettere, Carmine

    Journal of Cancer

    2023  Volume 14, Issue 7, Page(s) 1088–1106

    Abstract: The study of the biological effects of low-energy ultrasound and its applications is a rapidly expanding research area. Low-energy ultrasound could be used as anti-tumoral therapy with or without the pharmacological combination even if the second ... ...

    Abstract The study of the biological effects of low-energy ultrasound and its applications is a rapidly expanding research area. Low-energy ultrasound could be used as anti-tumoral therapy with or without the pharmacological combination even if the second situation has been scarcely investigated up to now. Very little information is available about the ultrasound effects on healthy red blood cells, CD3, and mainly CD8 subset lymphocytes which is the main subset cell having cytotoxic function towards cancer cells. In this study, we investigated
    Language English
    Publishing date 2023-04-24
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 2573318-7
    ISSN 1837-9664
    ISSN 1837-9664
    DOI 10.7150/jca.83050
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: RTG Signaling Sustains Mitochondrial Respiratory Capacity in HOG1-Dependent Osmoadaptation

    Guaragnella, Nicoletta / Agrimi, Gennaro / Scarcia, Pasquale / Suriano, Clelia / Pisano, Isabella / Bobba, Antonella / Mazzoni, Cristina / Palmieri, Luigi / Giannattasio, Sergio

    Microorganisms. 2021 Sept. 06, v. 9, no. 9

    2021  

    Abstract: Mitochondrial RTG-dependent retrograde signaling, whose regulators have been characterized in Saccharomyces cerevisiae, plays a recognized role under various environmental stresses. Of special significance, the activity of the transcriptional complex ... ...

    Abstract Mitochondrial RTG-dependent retrograde signaling, whose regulators have been characterized in Saccharomyces cerevisiae, plays a recognized role under various environmental stresses. Of special significance, the activity of the transcriptional complex Rtg1/3 has been shown to be modulated by Hog1, the master regulator of the high osmolarity glycerol pathway, in response to osmotic stress. The present work focuses on the role of RTG signaling in salt-induced osmotic stress and its interaction with HOG1. Wild-type and mutant cells, lacking HOG1 and/or RTG genes, are compared with respect to cell growth features, retrograde signaling activation and mitochondrial function in the presence and in the absence of high osmostress. We show that RTG2, the main upstream regulator of the RTG pathway, contributes to osmoadaptation in an HOG1-dependent manner and that, with RTG3, it is notably involved in a late phase of growth. Our data demonstrate that impairment of RTG signaling causes a decrease in mitochondrial respiratory capacity exclusively under osmostress. Overall, these results suggest that HOG1 and the RTG pathway may interact sequentially in the stress signaling cascade and that the RTG pathway may play a role in inter-organellar metabolic communication for osmoadaptation.
    Keywords Saccharomyces cerevisiae ; cell growth ; glycerol ; mitochondria ; mutants ; osmolarity ; osmotic stress ; osmotolerance ; transcription (genetics)
    Language English
    Dates of publication 2021-0906
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9091894
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: A comparative screening of laccase-mediator systems by white-rot fungi laccases for biocatalytic benzyl alcohol oxidation.

    Marino, Ivana / Pignataro, Eugenia / Danzi, Donatella / Cellini, Francesco / Cardellicchio, Cosimo / Biundo, Antonino / Pisano, Isabella / Capozzi, Maria Annunziata M

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 21602

    Abstract: Production of value-added compounds from waste materials is of utmost importance for the development of a sustainable society especially regarding their use as catalysts in industrially relevant synthetic reactions. Herein, we show the production of ... ...

    Abstract Production of value-added compounds from waste materials is of utmost importance for the development of a sustainable society especially regarding their use as catalysts in industrially relevant synthetic reactions. Herein, we show the production of laccases from four white-rot fungi, which were grown on agricultural residues, specifically Trametes versicolor 11269, Pleurotus ostreatus 1020, Panus tigrinus 707 and Lentinula edodes SC-495. The produced laccases were tested on a laccase-mediator system (LMS) for the biocatalytic oxidation of the model substrate benzyl alcohol into benzaldehyde. The LMS was carried out in the presence both of tetrahydrofuran as co-solvent and of the mediator 2,2,6,6-tetramethyl-1-piperidinyloxyl (TEMPO) due to its high redox potential and its ability to perform the oxidation. Tolerance studies showed that the dialyzed solutions were able to tolerate 1% (99:1 v/v) of co-solvent, whereas a concentration of 10% v/v had a detrimental activity. Performances in the biocatalytic oxidation of laccase solutions from different purification steps were compared. Similar conversion was observed for laccase in dialysis (raw) and gel filtration (GF) product versus commercial T. versicolor laccase. The latter oxidized almost 99% of substrate while the other laccase solutions were able to reach a conversion from 91% for the laccase solution from P. tigrinus 707 after dialysis, to 50% for the laccase solution from P. ostreatus 1020 after gel filtration. This work highlights the potential of unpurified laccase solutions to be used as catalysts in synthetic reactions.
    MeSH term(s) Laccase/metabolism ; Trametes/metabolism ; Benzyl Alcohol ; Renal Dialysis ; Oxidation-Reduction ; Solvents
    Chemical Substances Laccase (EC 1.10.3.2) ; Benzyl Alcohol (LKG8494WBH) ; Solvents
    Language English
    Publishing date 2022-12-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-24839-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Mitochondrial Role in Intrinsic Apoptosis Induced by a New Synthesized Chalcone in Hepatocellular Carcinoma Cells.

    Santarsiero, Anna / Pappalardo, Ilaria / Rosa, Gabriella Margherita / Pisano, Isabella / Superchi, Stefano / Convertini, Paolo / Todisco, Simona / Scafato, Patrizia / Infantino, Vittoria

    Biomedicines

    2022  Volume 10, Issue 12

    Abstract: Hepatocellular carcinoma (HCC) is the most common type of liver cancer and the fourth cause of cancer-related deaths worldwide. Presently, a few drugs are available for HCC treatment and prevention, including both natural and synthetic compounds. In this ...

    Abstract Hepatocellular carcinoma (HCC) is the most common type of liver cancer and the fourth cause of cancer-related deaths worldwide. Presently, a few drugs are available for HCC treatment and prevention, including both natural and synthetic compounds. In this study, a new chalcone, (
    Language English
    Publishing date 2022-12-02
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720867-9
    ISSN 2227-9059
    ISSN 2227-9059
    DOI 10.3390/biomedicines10123120
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Inactivation of

    Di Noia, Maria Antonietta / Scarcia, Pasquale / Agrimi, Gennaro / Ocheja, Ohiemi Benjamin / Wahid, Ehtisham / Pisano, Isabella / Paradies, Eleonora / Palmieri, Luigi / Guaragnella, Cataldo / Guaragnella, Nicoletta

    International journal of molecular sciences

    2023  Volume 24, Issue 6

    Abstract: ... ...

    Abstract Mitochondrial
    MeSH term(s) Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism ; Citric Acid Cycle/genetics ; Citrate (si)-Synthase/metabolism ; Signal Transduction ; Gene Expression Regulation, Fungal
    Chemical Substances Saccharomyces cerevisiae Proteins ; Citrate (si)-Synthase (EC 2.3.3.1)
    Language English
    Publishing date 2023-03-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24065320
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Genetic inactivation of the Carnitine/Acetyl-Carnitine mitochondrial carrier of Yarrowia lipolytica leads to enhanced odd-chain fatty acid production.

    Messina, Eugenia / de Souza, Camilla Pires / Cappella, Claudia / Barile, Simona Nicole / Scarcia, Pasquale / Pisano, Isabella / Palmieri, Luigi / Nicaud, Jean-Marc / Agrimi, Gennaro

    Microbial cell factories

    2023  Volume 22, Issue 1, Page(s) 128

    Abstract: Background: Mitochondrial carriers (MCs) can deeply affect the intracellular flux distribution of metabolic pathways. The manipulation of their expression level, to redirect the flux toward the production of a molecule of interest, is an attractive ... ...

    Abstract Background: Mitochondrial carriers (MCs) can deeply affect the intracellular flux distribution of metabolic pathways. The manipulation of their expression level, to redirect the flux toward the production of a molecule of interest, is an attractive target for the metabolic engineering of eukaryotic microorganisms. The non-conventional yeast Yarrowia lipolytica is able to use a wide range of substrates. As oleaginous yeast, it directs most of the acetyl-CoA therefrom generated towards the synthesis of lipids, which occurs in the cytoplasm. Among them, the odd-chain fatty acids (OCFAs) are promising microbial-based compounds with several applications in the medical, cosmetic, chemical and agricultural industries.
    Results: In this study, we have identified the MC involved in the Carnitine/Acetyl-Carnitine shuttle in Y. lipolytica, YlCrc1. The Y. lipolytica Ylcrc1 knock-out strain failed to grow on ethanol, acetate and oleic acid, demonstrating the fundamental role of this MC in the transport of acetyl-CoA from peroxisomes and cytoplasm into mitochondria. A metabolic engineering strategy involving the deletion of YlCRC1, and the recombinant expression of propionyl-CoA transferase from Ralstonia eutropha (RePCT), improved propionate utilization and its conversion into OCFAs. These genetic modifications and a lipogenic medium supplemented with glucose and propionate as the sole carbon sources, led to enhanced accumulation of OCFAs in Y. lipolytica.
    Conclusions: The Carnitine/Acetyl-Carnitine shuttle of Y. lipolytica involving YlCrc1, is the sole pathway for transporting peroxisomal or cytosolic acetyl-CoA to mitochondria. Manipulation of this carrier can be a promising target for metabolic engineering approaches involving cytosolic acetyl-CoA, as demonstrated by the effect of YlCRC1 deletion on OCFAs synthesis.
    MeSH term(s) Acetyl Coenzyme A/metabolism ; Carnitine/metabolism ; Acetylcarnitine/metabolism ; Yarrowia/genetics ; Yarrowia/metabolism ; Fatty Acids/metabolism ; Propionates/metabolism ; Mitochondria/metabolism ; Metabolic Engineering
    Chemical Substances Acetyl Coenzyme A (72-89-9) ; Carnitine (S7UI8SM58A) ; Acetylcarnitine (6DH1W9VH8Q) ; Fatty Acids ; Propionates
    Language English
    Publishing date 2023-07-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 2091377-1
    ISSN 1475-2859 ; 1475-2859
    ISSN (online) 1475-2859
    ISSN 1475-2859
    DOI 10.1186/s12934-023-02137-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: RTG

    Guaragnella, Nicoletta / Agrimi, Gennaro / Scarcia, Pasquale / Suriano, Clelia / Pisano, Isabella / Bobba, Antonella / Mazzoni, Cristina / Palmieri, Luigi / Giannattasio, Sergio

    Microorganisms

    2021  Volume 9, Issue 9

    Abstract: ... ...

    Abstract Mitochondrial
    Language English
    Publishing date 2021-09-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9091894
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Corrigendum: Bioisosteric Modification of To042: Synthesis and Evaluation of Promising Use-Dependent Inhibitors of Voltage-Gated Sodium Channels.

    Milani, Gualtiero / Cavalluzzi, Maria Maddalena / Altamura, Concetta / Santoro, Antonella / Perrone, Mariagrazia / Muraglia, Marilena / Colabufo, Nicola Antonio / Corbo, Filomena / Casalino, Elisabetta / Franchini, Carlo / Pisano, Isabella / Desaphy, Jean-François / Carrieri, Antonio / Carocci, Alessia / Lentini, Giovanni

    ChemMedChem

    2022  Volume 17, Issue 7, Page(s) e202200131

    Language English
    Publishing date 2022-03-21
    Publishing country Germany
    Document type Published Erratum
    ZDB-ID 2218496-X
    ISSN 1860-7187 ; 1860-7179
    ISSN (online) 1860-7187
    ISSN 1860-7179
    DOI 10.1002/cmdc.202200131
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 6280: Show issues Location:
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  10. Article ; Online: Regio- and stereoselective biocatalytic hydration of fatty acids from waste cooking oils en route to hydroxy fatty acids and bio-based polyesters.

    Biundo, Antonino / Stamm, Arne / Gorgoglione, Ruggiero / Syrén, Per-Olof / Curia, Silvio / Hauer, Bernhard / Capriati, Vito / Vitale, Paola / Perna, Filippo / Agrimi, Gennaro / Pisano, Isabella

    Enzyme and microbial technology

    2022  Volume 163, Page(s) 110164

    Abstract: The development of biorefinery approaches is of great relevance for the sustainable production of valuable compounds. In accordance with circular economy principles, waste cooking oils (WCOs) are renewable resources and biorefinery feedstocks, which ... ...

    Abstract The development of biorefinery approaches is of great relevance for the sustainable production of valuable compounds. In accordance with circular economy principles, waste cooking oils (WCOs) are renewable resources and biorefinery feedstocks, which contribute to a reduced impact on the environment. Frequently, this waste is wrongly disposed of into municipal sewage systems, thereby creating problems for the environment and increasing treatment costs in wastewater treatment plants. In this study, regenerated WCOs, which were intended for the production of biofuels, were transformed through a chemo-enzymatic approach to produce hydroxy fatty acids, which were further used in polycondensation reaction for polyester production. Escherichia coli whole cell biocatalyst containing the recombinantly produced Elizabethkingia meningoseptica Oleate hydratase (Em_OhyA) was used for the biocatalytic hydration of crude WCOs-derived unsaturated free fatty acids for the production of hydroxy fatty acids. Further hydrogenation reaction and methylation of the crude mixture allowed the production of (R)- 10-hydroxystearic acid methyl ester that was further purified with a high purity (> 90%), at gram scale. The purified (R)- 10-hydroxystearic acid methyl ester was polymerized through a polycondensation reaction to produce the corresponding polyester. This work highlights the potential of waste products to obtain bio-based hydroxy fatty acids and polyesters through a biorefinery approach.
    Language English
    Publishing date 2022-11-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 423729-8
    ISSN 1879-0909 ; 0141-0229
    ISSN (online) 1879-0909
    ISSN 0141-0229
    DOI 10.1016/j.enzmictec.2022.110164
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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