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  1. Article ; Online: Morphological alterations in the prostate stroma of rats submitted to chronic nicotine treatment.

    Carvalho, Cesar Alexandre Fabrega / Pissolato, Mariana / Candido, Eduardo Marcelo / Liberti, Edson Aparecido / Cagnon, Valéria Helena Alves

    Microscopy research and technique

    2012  Volume 75, Issue 1, Page(s) 46–53

    Abstract: The stroma plays a fundamental role in the function of different glandular systems. In the prostate, the stroma is responsible for the development and maintenance of the differentiated state of the epithelium. Nicotine induces tobacco dependence and ... ...

    Abstract The stroma plays a fundamental role in the function of different glandular systems. In the prostate, the stroma is responsible for the development and maintenance of the differentiated state of the epithelium. Nicotine induces tobacco dependence and promotes morphological alterations in the epithelial compartment. However, its effects on the prostate stroma are unclear. The objective of this study was to evaluate the morphology of the stromal microenvironment in the ventral prostate lobe of rats submitted to chronic nicotine administration. Twenty rats (Rattus norvegicus) were divided into two groups: 10 animals received subcutaneous nicotine and 10 animals received physiological saline by the same route. After treatment, samples were collected from the ventral prostate lobe, processed and submitted to histology, histochemistry, and ultrastructural analysis by transmission and scanning electron microscopy. The level of circulating testosterone was also analyzed. The results showed a significant increase in the density of type I collagen (56.3% to 85.9%, P < 0.01) and a decrease in the density of type III collagen (43.7% to 14.1%, P < 0.01). In addition, there was a qualitative increase in elastic fibers and in the number of smooth muscle cells with a secretory phenotype. Circulating testosterone levels were significantly reduced (898.3 to 363.1 ng/mL, P < 0.01). The results showed that nicotine modifies different components of the prostate stroma, suggesting that this drug is a risk factor for morphofunctional alterations in the prostate gland.
    MeSH term(s) Animals ; Collagen/metabolism ; Humans ; Male ; Microscopy, Electron, Scanning ; Nicotine/administration & dosage ; Prostate/cytology ; Prostate/drug effects ; Prostate/metabolism ; Prostate/ultrastructure ; Rats ; Rats, Wistar ; Smoking/metabolism ; Stromal Cells/cytology ; Stromal Cells/drug effects ; Stromal Cells/metabolism ; Stromal Cells/ultrastructure ; Testosterone/metabolism
    Chemical Substances Testosterone (3XMK78S47O) ; Nicotine (6M3C89ZY6R) ; Collagen (9007-34-5)
    Language English
    Publishing date 2012-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1099714-3
    ISSN 1097-0029 ; 1059-910X
    ISSN (online) 1097-0029
    ISSN 1059-910X
    DOI 10.1002/jemt.21023
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  2. Article ; Online: Technical performance of a lateral flow immunoassay for detection of anti-SARS-CoV-2 IgG in the outpatient follow-up of non-severe cases and at different times after vaccination: comparison with enzyme and chemiluminescent immunoassays.

    Barreira, Gabriel Acca / Santos, Emilly Henrique Dos / Pereira, Maria Fernanda Bádue / Rodrigues, Karen Alessandra / Rocha, Mussya Cisotto / Kanunfre, Kelly Aparecida / Marques, Heloisa Helena de Sousa / Okay, Thelma Suely / Eisencraft, Adriana Pasmanik / Rossi Junior, Alfio / Fante, Alice Lima / Cora, Aline Pivetta / Costa Reis, Amelia Gorete A de / Ferrer, Ana Paula Scoleze / Andrade, Anarella Penha Meirelles de / Watanabe, Andreia / Gonçalves, Angelina Maria Freire / Waetge, Aurora Rosaria Pagliara / Silva, Camila Altenfelder /
    Ceneviva, Carina / Lazari, Carolina Dos Santos / Abellan, Deipara Monteiro / Sabino, Ester Cerdeira / Bianchini, Fabíola Roberta Marim / Alcantara, Flávio Ferraz de Paes / Ramos, Gabriel Frizzo / Leal, Gabriela Nunes / Rodriguez, Isadora Souza / Pinho, João Renato Rebello / Carneiro, Jorge David Avaizoglou / Paz, Jose Albino / Ferreira, Juliana Carvalho / Ferranti, Juliana Ferreira / Ferreira, Juliana de Oliveira Achili / Framil, Juliana Valéria de Souza / Silva, Katia Regina da / Bastos, Karina Lucio de Medeiros / Galleti, Karine Vusberg / Cristofani, Lilian Maria / Suzuki, Lisa / Campos, Lucia Maria Arruda / Perondi, Maria Beatriz de Moliterno / Diniz, Maria de Fatima Rodrigues / Fonseca, Maria Fernanda Mota / Cordon, Mariana Nutti de Almeida / Pissolato, Mariana / Peres, Marina Silva / Garanito, Marlene Pereira / Imamura, Marta / Dorna, Mayra de Barros / Luglio, Michele / Aikawa, Nadia Emi / Degaspare, Natalia Viu / Sakita, Neusa Keico / Udsen, Nicole Lee / Scudeller, Paula Gobi / Gaiolla, Paula Vieira de Vincenzi / Severini, Rafael da Silva Giannasi / Rodrigues, Regina Maria / Toma, Ricardo Katsuya / Paula, Ricardo Iunis Citrangulo de / Palmeira, Patricia / Forsait, Silvana / Farhat, Sylvia Costa Lima / Sakano, Tânia Miyuki Shimoda / Koch, Vera Hermina Kalika / Cobello Junior, Vilson

    Revista do Instituto de Medicina Tropical de Sao Paulo

    2022  Volume 64, Page(s) e49

    Abstract: This study assessed the technical performance of a rapid lateral flow immunochromatographic assay (LFIA) for the detection of anti-SARS-CoV-2 IgG and compared LFIA results with chemiluminescent immunoassay (CLIA) results and an in-house enzyme ... ...

    Abstract This study assessed the technical performance of a rapid lateral flow immunochromatographic assay (LFIA) for the detection of anti-SARS-CoV-2 IgG and compared LFIA results with chemiluminescent immunoassay (CLIA) results and an in-house enzyme immunoassay (EIA). To this end, a total of 216 whole blood or serum samples from three groups were analyzed: the first group was composed of 68 true negative cases corresponding to blood bank donors, healthy young volunteers, and eight pediatric patients diagnosed with other coronavirus infections. The serum samples from these participants were obtained and stored in a pre-COVID-19 period, thus they were not expected to have COVID-19. In the second group of true positive cases, we chose to replace natural cases of COVID-19 by 96 participants who were expected to have produced anti-SARS-CoV-2 IgG antibodies 30-60 days after the vaccine booster dose. The serum samples were collected on the same day that LFIA were tested either by EIA or CLIA. The third study group was composed of 52 participants (12 adults and 40 children) who did or did not have anti-SARS-CoV-2 IgG antibodies due to specific clinical scenarios. The 12 adults had been vaccinated more than seven months before LFIA testing, and the 40 children had non-severe COVID-19 diagnosed using RT-PCR during the acute phase of infection. They were referred for outpatient follow-up and during this period the serum samples were collected and tested by CLIA and LFIA. All tests were performed by the same healthcare operator and there was no variation of LFIA results when tests were performed on finger prick whole blood or serum samples, so that results were grouped for analysis. LFIA's sensitivity in detecting anti-SARS-CoV-2 IgG antibodies was 90%, specificity 97.6%, efficiency 93%, PPV 98.3%, NPV 86.6%, and likelihood ratio for a positive or a negative result were 37.5 and 0.01 respectively. There was a good agreement (Kappa index of 0.677) between LFIA results and serological (EIA or CLIA) results. In conclusion, LFIA analyzed in this study showed a good technical performance and agreement with reference serological assays (EIA or CLIA), therefore it can be recommended for use in the outpatient follow-up of non-severe cases of COVID-19 and to assess anti-SARS-CoV-2 IgG antibody production induced by vaccination and the antibodies decrease over time. However, LFIAs should be confirmed by using reference serological assays whenever possible.
    MeSH term(s) Adult ; Antibodies, Viral ; COVID-19/diagnosis ; COVID-19/prevention & control ; Child ; Follow-Up Studies ; Humans ; Immunoassay/methods ; Immunoglobulin G ; Immunoglobulin M ; Outpatients ; Sensitivity and Specificity ; Vaccination
    Chemical Substances Antibodies, Viral ; Immunoglobulin G ; Immunoglobulin M
    Language English
    Publishing date 2022-07-13
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 128928-7
    ISSN 1678-9946 ; 0036-4665
    ISSN (online) 1678-9946
    ISSN 0036-4665
    DOI 10.1590/S1678-9946202264049
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 292: Show issues Location:
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  3. Article ; Online: Differences in children and adolescents with SARS-CoV-2 infection: a cohort study in a Brazilian tertiary referral hospital.

    Marques, Heloisa Helena de Sousa / Pereira, Maria Fernanda Badue / Santos, Angélica Carreira Dos / Fink, Thais Toledo / Paula, Camila Sanson Yoshino de / Litvinov, Nadia / Schvartsman, Claudio / Delgado, Artur Figueiredo / Gibelli, Maria Augusta Bento Cicaroni / Carvalho, Werther Brunow de / Odone Filho, Vicente / Tannuri, Uenis / Carneiro-Sampaio, Magda / Grisi, Sandra / Duarte, Alberto José da Silva / Antonangelo, Leila / Francisco, Rossana Pucineli Vieira / Okay, Thelma Suely / Batisttella, Linamara Rizzo /
    Carvalho, Carlos Roberto Ribeiro de / Brentani, Alexandra Valéria Maria / Silva, Clovis Artur / Eisencraft, Adriana Pasmanik / Rossi Junior, Alfio / Fante, Alice Lima / Cora, Aline Pivetta / Reis, Amelia Gorete A de Costa / Ferrer, Ana Paula Scoleze / Andrade, Anarella Penha Meirelles de / Watanabe, Andreia / Gonçalves, Angelina Maria Freire / Waetge, Aurora Rosaria Pagliara / Silva, Camila Altenfelder / Ceneviva, Carina / Lazari, Carolina Dos Santos / Abellan, Deipara Monteiro / Santos, Emilly Henrique Dos / Sabino, Ester Cerdeira / Bianchini, Fabíola Roberta Marim / Alcantara, Flávio Ferraz de Paes / Ramos, Gabriel Frizzo / Leal, Gabriela Nunes / Rodriguez, Isadora Souza / Pinho, João Renato Rebello / Carneiro, Jorge David Avaizoglou / Paz, Jose Albino / Ferreira, Juliana Carvalho / Ferranti, Juliana Ferreira / Ferreira, Juliana de Oliveira Achili / Framil, Juliana Valéria de Souza / Silva, Katia Regina da / Kanunfre, Kelly Aparecida / Bastos, Karina Lucio de Medeiros / Galleti, Karine Vusberg / Cristofani, Lilian Maria / Suzuki, Lisa / Campos, Lucia Maria Arruda / Perondi, Maria Beatriz de Moliterno / Diniz, Maria de Fatima Rodrigues / Fonseca, Maria Fernanda Mota / Cordon, Mariana Nutti de Almeida / Pissolato, Mariana / Peres, Marina Silva / Garanito, Marlene Pereira / Imamura, Marta / Dorna, Mayra de Barros / Luglio, Michele / Rocha, Mussya Cisotto / Aikawa, Nadia Emi / Degaspare, Natalia Viu / Sakita, Neusa Keico / Udsen, Nicole Lee / Scudeller, Paula Gobi / Gaiolla, Paula Vieira de Vincenzi / Severini, Rafael da Silva Giannasi / Rodrigues, Regina Maria / Toma, Ricardo Katsuya / Paula, Ricardo Iunis Citrangulo de / Palmeira, Patricia / Forsait, Silvana / Farhat, Sylvia Costa Lima / Sakano, Tânia Miyuki Shimoda / Koch, Vera Hermina Kalika / Cobello Junior, Vilson

    Clinics (Sao Paulo, Brazil)

    2021  Volume 76, Page(s) e3488

    Abstract: Objectives: To compare demographic/clinical/laboratory/treatments and outcomes among children and adolescents with laboratory-confirmed coronavirus disease 2019 (COVID-19).: Methods: This was a cross-sectional study that included patients diagnosed ... ...

    Abstract Objectives: To compare demographic/clinical/laboratory/treatments and outcomes among children and adolescents with laboratory-confirmed coronavirus disease 2019 (COVID-19).
    Methods: This was a cross-sectional study that included patients diagnosed with pediatric COVID-19 (aged <18 years) between April 11, 2020 and April 22, 2021. During this period, 102/5,951 (1.7%) of all admissions occurred in neonates, children, and adolescents. Furthermore, 3,962 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection samples were processed in patients aged <18 years, and laboratory-confirmed COVID-19 occurred in 155 (4%) inpatients and outpatients. Six/155 pediatric patients were excluded from the study. Therefore, the final group included 149 children and adolescents (n=97 inpatients and 52 outpatients) with positive SARS-CoV-2 results.
    Results: The frequencies of sore throat, anosmia, dysgeusia, headache, myalgia, nausea, lymphopenia, pre-existing chronic conditions, immunosuppressive conditions, and autoimmune diseases were significantly reduced in children and adolescents (p<0.05). Likewise, the frequencies of enoxaparin use (p=0.037), current immunosuppressant use (p=0.008), vasoactive agents (p=0.045), arterial hypotension (p<0.001), and shock (p=0.024) were significantly lower in children than in adolescents. Logistic regression analysis showed that adolescents with laboratory-confirmed COVID-19 had increased odds ratios (ORs) for sore throat (OR 13.054; 95% confidence interval [CI] 2.750-61.977; p=0.001), nausea (OR 8.875; 95% CI 1.660-47.446; p=0.011), and lymphopenia (OR 3.575; 95% CI 1.355-9.430; p=0.010), but also had less hospitalizations (OR 0.355; 95% CI 0.138-0.916; p=0.032). The additional logistic regression analysis on patients with preexisting chronic conditions (n=108) showed that death as an outcome was significantly associated with pediatric severe acute respiratory syndrome (SARS) (OR 22.300; 95% CI 2.341-212.421; p=0.007) and multisystem inflammatory syndrome in children (MIS-C) (OR 11.261; 95% CI 1.189-106. 581; p=0.035).
    Conclusions: Half of the laboratory-confirmed COVID-19 cases occurred in adolescents. Individuals belonging to this age group had an acute systemic involvement of SARS-CoV-2 infection. Pediatric SARS and MIS-C were the most important factors associated with the mortality rate in pediatric chronic conditions with COVID-19.
    MeSH term(s) Adolescent ; COVID-19/complications ; Child ; Cohort Studies ; Cross-Sectional Studies ; Humans ; Infant, Newborn ; SARS-CoV-2 ; Systemic Inflammatory Response Syndrome ; Tertiary Care Centers
    Language English
    Publishing date 2021-11-26
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2182801-5
    ISSN 1980-5322 ; 1807-5932
    ISSN (online) 1980-5322
    ISSN 1807-5932
    DOI 10.6061/clinics/2021/e3488
    Database MEDical Literature Analysis and Retrieval System OnLINE

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