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Article ; Online: Irisin: A Possible Marker of Adipose Tissue Dysfunction in Obesity.

Tomasello, Laura / Pitrone, Maria / Guarnotta, Valentina / Giordano, Carla / Pizzolanti, Giuseppe

International journal of molecular sciences

2023 Volume 24, Issue 15

Abstract: Adipose tissue (AT) secretes pro- and anti-inflammatory cytokines involved in AT homeostasis, including tumor necrosis factor-α (TNFα) and irisin. The functionality of AT is based on a regulated equilibrium between adipogenesis and extracellular matrix ( ... ...

Abstract	Adipose tissue (AT) secretes pro- and anti-inflammatory cytokines involved in AT homeostasis, including tumor necrosis factor-α (TNFα) and irisin. The functionality of AT is based on a regulated equilibrium between adipogenesis and extracellular matrix (ECM) remodeling. We investigated the contributions of adipose progenitors (ASCs) and adipocytes (AMCs) to TNFα-induced ECM remodeling and a possible implication of irisin in AT impairment in obesity. ASCs and AMCs were exposed to TNFα treatment and nuclear factor-kappa (NF-kB) pathway was investigated: Tissue Inhibitor of Metalloproteinase (TIMP-1), Twist Family Transcription Factor 1 (TWIST-1), and peroxisome proliferator-activated receptor-γ (PPARγ) expression levels were analyzed. The proteolytic activity of matrix metalloproteinases (MMPs) -2 and -9 was analyzed by zymography, and the irisin protein content was measured by ELISA. In inflamed AMCs, a TIMP-1/TWIST-1 imbalance leads to a drop in PPARγ. Adipogenesis and lipid storage ability impairment come with local tissue remodeling due to MMP-9 overactivation. In vitro and ex vivo measurements confirm positive correlations among inflammation, adipose secreting irisin levels, and circulating irisin levels in patients with visceral obesity. Our findings identify the NF-kB downstream effectors as molecular initiators of AT dysfunction and suggest irisin as a possible AT damage and obesity predictive factor.
MeSH term(s)	Humans ; Adipose Tissue/metabolism ; Fibronectins/metabolism ; NF-kappa B/metabolism ; Obesity/metabolism ; PPAR gamma/genetics ; PPAR gamma/metabolism ; Tissue Inhibitor of Metalloproteinase-1/metabolism ; Tumor Necrosis Factor-alpha/metabolism
Chemical Substances	Fibronectins ; NF-kappa B ; PPAR gamma ; Tissue Inhibitor of Metalloproteinase-1 ; Tumor Necrosis Factor-alpha ; FNDC5 protein, human
Language	English
Publishing date	2023-07-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms241512082
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Circulating Irisin Levels as a Marker of Osteosarcopenic-Obesity in Cushing's Disease.

Guarnotta, Valentina / Prinzi, Antonio / Pitrone, Maria / Pizzolanti, Giuseppe / Giordano, Carla

Diabetes, metabolic syndrome and obesity : targets and therapy

2020 Volume 13, Page(s) 1565–1574

Abstract: Purpose: To evaluate circulating irisin levels in patients with active and controlled Cushing's disease (CD).: Design: Forty-four patients with CD evaluated during the active phase and after 12 months of biochemical remission and 40 controls were ... ...

Abstract	Purpose: To evaluate circulating irisin levels in patients with active and controlled Cushing's disease (CD). Design: Forty-four patients with CD evaluated during the active phase and after 12 months of biochemical remission and 40 controls were recruited. Methods: Phenotypic, anthropometric, hormonal and metabolic parameters, including insulin sensitivity estimation by homeostatic model of insulin resistance, Matsuda index and oral disposition index and circulating irisin levels were evaluated. Results: Patients with active CD showed lower irisin levels compared to controls (p<0.001) and controlled CD (p<0.001). The independent variables significantly associated with irisin were waist circumference (WC) (p=0.025), body fat percentage (BF%) (p=0.009), PTH (p=0.007) and chair rising test (CRT) (p<0.001) in active CD and WC (p=0.013), BF% (p=0.014), PTH (p=0.038), CRT (p=0.029) and urinary-free cortisol (p<0.001) in controlled CD. Conclusion: Circulating irisin levels tend to be lower in patients with active CD compared to those with controlled CD and controls. They are strongly associated with osteosarcopenia and central obesity in CD and therefore may be a possible marker of diagnosis.
Language	English
Publishing date	2020-05-11
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2494854-8
ISSN	1178-7007
ISSN	1178-7007
DOI	10.2147/DMSO.S249090
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Article ; Online: L'evoluzione dell'emergenza

Pitrone, Maria Concetta / Martire, Fabrizio / Pastore, Sara

2020

Abstract: Il saggio analizza l'evoluzione delle rappresentazioni e degli atteggiamenti di un campione di residenti in Italia durante il periodo di lockdown (marzo-aprile 2020) ...

Abstract	Il saggio analizza l'evoluzione delle rappresentazioni e degli atteggiamenti di un campione di residenti in Italia durante il periodo di lockdown (marzo-aprile 2020)
Keywords	survey ; atteggiamenti ; rappresentazioni ; lockdown ; covid19
Language	Italian
Publisher	FrancoAngeli
Publishing country	it
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Knockdown of NANOG Reduces Cell Proliferation and Induces G0/G1 Cell Cycle Arrest in Human Adipose Stem Cells.

Pitrone, Maria / Pizzolanti, Giuseppe / Coppola, Antonina / Tomasello, Laura / Martorana, Stefania / Pantuso, Gianni / Giordano, Carla

International journal of molecular sciences

2019 Volume 20, Issue 10

Abstract: The core components of regenerative medicine are stem cells with high self-renewal and tissue regeneration potentials. Adult stem cells can be obtained from many organs and tissues. ...

Abstract	The core components of regenerative medicine are stem cells with high self-renewal and tissue regeneration potentials. Adult stem cells can be obtained from many organs and tissues.
MeSH term(s)	Adult ; Cell Differentiation ; Cell Proliferation ; Cell Self Renewal ; Cells, Cultured ; Down-Regulation ; Female ; G1 Phase Cell Cycle Checkpoints ; Gene Knockdown Techniques ; Humans ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/metabolism ; Middle Aged ; Nanog Homeobox Protein/genetics
Chemical Substances	NANOG protein, human ; Nanog Homeobox Protein
Language	English
Publishing date	2019-05-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms20102580
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Article ; Online: PFN1 and integrin-β1/mTOR axis involvement in cornea differentiation of fibroblast limbal stem cells.

Tomasello, Laura / Coppola, Antonina / Pitrone, Maria / Failla, Valentina / Cillino, Salvatore / Pizzolanti, Giuseppe / Giordano, Carla

Journal of cellular and molecular medicine

2019 Volume 23, Issue 11, Page(s) 7210–7221

Abstract: Ex vivo limbal stem cell transplantation is the main therapeutic approach to address a complete and functional re-epithelialization in corneal blindness, the second most common eye disorder. Although important key points were defined, the molecular ... ...

Abstract	Ex vivo limbal stem cell transplantation is the main therapeutic approach to address a complete and functional re-epithelialization in corneal blindness, the second most common eye disorder. Although important key points were defined, the molecular mechanisms involved in the epithelial phenotype determination are unclear. Our previous studies have demonstrated the pluripotency and immune-modulatory of fibroblast limbal stem cells (f-LSCs), isolated from the corneal limbus. We defined a proteomic profile especially enriched in wound healing and cytoskeleton-remodelling proteins, including Profilin-1 (PFN1). In this study we postulate that pfn-1 knock down promotes epithelial lineage by inhibiting the integrin-β1(CD29)/mTOR pathway and subsequent NANOG down-expression. We showed that it is possible modulate pfn1 expression levels by treating f-LSCs with Resveratrol (RSV), a natural compound: pfn1 decline is accompanied with up-regulation of the specific differentiation epithelial genes pax6 (paired-box 6), sox17 (sex determining region Y-box 17) and ΔNp63-α (p63 splice variant), consistent with drop-down of the principle stem gene levels. These results contribute to understand the molecular biology of corneal epithelium development and suggest that pfn1 is a potential molecular target for the treatment of corneal blindness based on epithelial cell dysfunction.
MeSH term(s)	Apoptosis ; Biomarkers/metabolism ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Epithelium, Corneal/cytology ; Epithelium, Corneal/metabolism ; Fibroblasts/cytology ; Fibroblasts/metabolism ; Gene Expression Regulation ; Humans ; Integrin beta1/genetics ; Integrin beta1/metabolism ; Limbus Corneae/cytology ; Limbus Corneae/metabolism ; Profilins/genetics ; Profilins/metabolism ; Stem Cells/cytology ; Stem Cells/metabolism ; TOR Serine-Threonine Kinases/genetics ; TOR Serine-Threonine Kinases/metabolism ; Wound Healing
Chemical Substances	Biomarkers ; Integrin beta1 ; PFN1 protein, human ; Profilins ; MTOR protein, human (EC 2.7.1.1) ; TOR Serine-Threonine Kinases (EC 2.7.1.1)
Language	English
Publishing date	2019-09-12
Publishing country	England
Document type	Journal Article
ZDB-ID	2074559-X
ISSN	1582-4934 ; 1582-4934 ; 1582-1838
ISSN (online)	1582-4934
ISSN	1582-4934 ; 1582-1838
DOI	10.1111/jcmm.14438
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Article ; Online: Pasireotide versus pituitary surgery: a retrospective analysis of 12 months of treatment in patients with Cushing's disease.

Guarnotta, Valentina / Ciresi, Alessandro / Pitrone, Maria / Pizzolanti, Giuseppe / Giordano, Carla

Endocrine

2017 Volume 59, Issue 2, Page(s) 454–457

MeSH term(s)	Adult ; Female ; Hormones/therapeutic use ; Humans ; Male ; Middle Aged ; Neurosurgical Procedures ; Pituitary ACTH Hypersecretion/drug therapy ; Pituitary ACTH Hypersecretion/surgery ; Pituitary ACTH Hypersecretion/therapy ; Pituitary Gland/surgery ; Retrospective Studies ; Somatostatin/analogs & derivatives ; Somatostatin/therapeutic use
Chemical Substances	Hormones ; Somatostatin (51110-01-1) ; pasireotide (98H1T17066)
Language	English
Publishing date	2017-03-17
Publishing country	United States
Document type	Comparative Study ; Letter
ZDB-ID	1194484-5
ISSN	1559-0100 ; 1355-008X ; 0969-711X
ISSN (online)	1559-0100
ISSN	1355-008X ; 0969-711X
DOI	10.1007/s12020-017-1276-7
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Article: Growth and Osteogenic Differentiation of Discarded Gingiva-Derived Mesenchymal Stem Cells on a Commercial Scaffold.

Cristaldi, Marta / Mauceri, Rodolfo / Campisi, Giuseppina / Pizzo, Giuseppe / Alessandro, Riccardo / Tomasello, Laura / Pitrone, Maria / Pizzolanti, Giuseppe / Giordano, Carla

Frontiers in cell and developmental biology

2020 Volume 8, Page(s) 292

Abstract: Background: In periodontal patients with jawbone resorption, the autologous bone graft is considered a "gold standard" procedure for the placing of dental prosthesis; however, this procedure is a costly intervention and poses the risk of clinical ... ...

Abstract	Background: In periodontal patients with jawbone resorption, the autologous bone graft is considered a "gold standard" procedure for the placing of dental prosthesis; however, this procedure is a costly intervention and poses the risk of clinical complications. Thanks to the use of adult mesenchymal stem cells, smart biomaterials, and active biomolecules, regenerative medicine and bone tissue engineering represent a valid alternative to the traditional procedures. Aims: In the past, mesenchymal stem cells isolated from periodontally compromised gingiva were considered a biological waste and discarded during surgical procedures. This study aims to test the osteoconductive activity of FISIOGRAFT Bone Granular Materials and methods: We isolated human mesenchymal stem cells from 22 healthy and 26 periodontally compromised gingival biopsy tissues and confirmed the stem cell phenotype by doubling time assay, colony-forming unit assay, and expression of surface and nuclear mesenchymal stem cell markers, respectively by cytofluorimetry and real-time quantitative PCR. Healthy and periodontally compromised gingival mesenchymal stem cells were seeded on FISIOGRAFT Bone Granular Results: Even though preliminary, the results demonstrate that FISIOGRAFT Bone Granular Conclusion: Matriderm
Language	English
Publishing date	2020-05-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2737824-X
ISSN	2296-634X
ISSN	2296-634X
DOI	10.3389/fcell.2020.00292
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Article ; Online: Human limbal fibroblast-like stem cells induce immune-tolerance in autoreactive T lymphocytes from female patients with Hashimoto's thyroiditis.

Coppola, Antonina / Tomasello, Laura / Pitrone, Maria / Cillino, Salvatore / Richiusa, Pierina / Pizzolanti, Giuseppe / Giordano, Carla

Stem cell research & therapy

2017 Volume 8, Issue 1, Page(s) 154

Abstract: Background: Due to their "natural immune privilege" and immunoregulatory properties human fibroblast-like limbal stem cells (f-LSCs) have acquired great interest as a potential tool for achieving immunotolerance. Hashimoto's thyroiditis (HT) is the most ...

Abstract	Background: Due to their "natural immune privilege" and immunoregulatory properties human fibroblast-like limbal stem cells (f-LSCs) have acquired great interest as a potential tool for achieving immunotolerance. Hashimoto's thyroiditis (HT) is the most common thyroid autoimmune disease and cause of hypothyroidism. To date, conventional hormone replacement therapy and unspecific immunosuppressive regimens cannot provide a definitive cure for HT subjects. We explored the immunosuppressant potential of human f-LSCs on circulating lymphomonocytes (PBMCs) collected from healthy donors and female HT patients. Methods: We assessed the immunophenotyping of f-LSCs, both untreated and after 48 h of proinflammatory cytokine exposure, by means of quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and flow cytometry. The immunosuppressant effects of f-LSCs on healthy activated PBMCs were investigated in cell-cell contact and transwell settings through cell cycle assay, acridine orange staining, and caspase-3 detection. We also studied T-cell responses and possible Treg conversion by means of flow cytometry. Functional assays were conducted in activated HT lymphocytes cocultured with f-LSCs after carboxyfluorescein succinimidyl ester labeling and intracellular detection of pro- and anti-inflammatory cytokines. Results: The hypo-immunogenicity of the f-LSC population depended on both cell contact and soluble factors produced, as well as the undetectable expression of all those molecules required to fully activate T lymphocytes. Following exposure to Th1 cytokines, f-LSCs augmented expression of programmed death-ligand 1 and 2 (PDL-1 and -2), indoleamine-pyrrole-2,3-dioxygenase (IDO), interleukin (IL)-6, and monocyte chemotactic protein 1 (MCP-1) while maintaining their negative phenotype for major histocompatibility (MHC) class II and costimulatory molecules. During coculture, f-LSCs suppressed up to 40% of proliferation in healthy activated PBMCs, arrested them in the G0/G1 cell cycle phase without inducing apoptosis cascade, inverted the CD4/CD8 ratio, and promoted sustained expression of the immunomodulator marker CD69. Under coculture conditions the Th imbalance of autoreactive T cells from female HT patients was fully restored. Conclusions: Our study describes an in vitro coculture system able to prevent inappropriate activation of autoreactive T lymphocytes of female HT patients and to generate a tolerogenic environment even in an inflammatory background. Further investigations are necessary to establish whether this stem cell-based therapy approach in HT could avoid lifetime hormone replacement therapy by inducing T-cell education.
MeSH term(s)	Adult ; Aged ; CD8-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/pathology ; Cells, Cultured ; Cytokines/immunology ; Female ; Fibroblasts/immunology ; Fibroblasts/physiology ; Hashimoto Disease/immunology ; Hashimoto Disease/pathology ; Humans ; Immune Tolerance ; Middle Aged ; Stem Cells/immunology ; Stem Cells/pathology ; Th1 Cells/immunology ; Th1 Cells/pathology
Chemical Substances	Cytokines
Language	English
Publishing date	2017--03
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2548671-8
ISSN	1757-6512 ; 1757-6512
ISSN (online)	1757-6512
ISSN	1757-6512
DOI	10.1186/s13287-017-0611-5
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Article: Anti-Inflammatory Action of Heterogeneous Nuclear Ribonucleoprotein A2/B1 in Patients with Autoimmune Endocrine Disorders.

Coppola, Antonina / Cancemi, Patrizia / Tomasello, Laura / Guarnotta, Valentina / Pitrone, Maria / Failla, Valentina / Cillino, Salvatore / Feo, Salvatore / Pizzolanti, Giuseppe / Giordano, Carla

Journal of clinical medicine

2019 Volume 9, Issue 1

Abstract: Our previous studies documented that human fibroblast-limbal stem cells (f-LSCs) possess immunosuppressive capabilities, playing a role in regulating T-cell activity. This study highlights the molecular activities by which human f-LSCs can attenuate the ... ...

Abstract	Our previous studies documented that human fibroblast-limbal stem cells (f-LSCs) possess immunosuppressive capabilities, playing a role in regulating T-cell activity. This study highlights the molecular activities by which human f-LSCs can attenuate the inflammatory responses of self-reactive peripheral blood mononuclear cells (PBMCs) collected from patients with autoimmune endocrine diseases (AEDs). Anti-CD3 activated PBMCs from twenty healthy donors and fifty-two patients with AEDs were cocultured on f-LSC monolayer. 2D-DIGE proteomic experiments, mass spectrometry sequencing and functional in vitro assays were assessed in cocultured PBMCs. We identified the downmodulation of several human heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) isoforms in healthy and AED activated PBMCs upon f-LSC interaction. The reduction of hnRNPA2/B1 protein expression largely affected the cycling ki67
Language	English
Publishing date	2019-12-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662592-1
ISSN	2077-0383
ISSN	2077-0383
DOI	10.3390/jcm9010009
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Article ; Online: Cut-off points of the visceral adiposity index (VAI) identifying a visceral adipose dysfunction associated with cardiometabolic risk in a Caucasian Sicilian population

Amato Marco C / Giordano Carla / Pitrone Maria / Galluzzo Aldo

Lipids in Health and Disease, Vol 10, Iss 1, p

2011 Volume 183

Abstract: Abstract Background The Visceral Adiposity Index (VAI) is a sex-specific mathematical index, based on Waist Circumference (WC), Body Mass Index (BMI), triglycerides (TG) and HDL cholesterol (HDL) levels, indirectly expressing visceral adipose function ... ...

Abstract	Abstract Background The Visceral Adiposity Index (VAI) is a sex-specific mathematical index, based on Waist Circumference (WC), Body Mass Index (BMI), triglycerides (TG) and HDL cholesterol (HDL) levels, indirectly expressing visceral adipose function and insulin sensitivity. Our aim was to find the optimal cut-off points of VAI identifying a visceral adipose dysfunction (VAD) associated with cardiometabolic risk in a Caucasian Sicilian population. Methods Medical check-up data of 1,764 Primary Care patients (PC patients) were retrospectively and cross-sectionally examined using a receiver-operating characteristic (ROC) curve to determine appropriate stratified-for-age cut-off of VAI, for the identification of PC patients with Metabolic Syndrome (MetS) according to the NCEP-ATP III criteria. The PC patients with higher VAI scores were subdivided into three groups according to VAI tertiles (i.e. PC patients with mild VAD, moderate VAD or severe VAD). Finally, VAD classes were compared to classical cardio- and cerebrovascular risk factors as independent predictors of coronary heart disease and/or myocardial infarction, transient ischemic attack and/or ischemic stroke. Results Moderate and severe VADs proved to be independently associated with cardiovascular events [(OR: 5.35; 95% CI: 1.92-14.87; p = 0.001) and (OR: 7.46; 95% CI: 2.64-21.05; p < 0.001) respectively]. Mild, moderate and severe VADs were found to be independently associated with cerebrovascular events [(OR: 2.73; 95% CI: 1.12-6.65; p = 0.027), (OR: 4.20; 95% CI: 1.86-9.45; p = 0.001) and (OR: 5.10; 95% CI: 2.14-12.17; p < 0.001) respectively]. Conclusions Our study suggests that among Caucasian Sicilian subjects there are clear cut-off points of VAI able to identify a VAD strongly associated with cardiometabolic risk.
Keywords	Obesity ; cardiometabolic risk ; visceral adipose dynsfunction ; VAI ; Physiology ; QP1-981 ; Science ; Q ; DOAJ:Physiology ; DOAJ:Biology ; DOAJ:Biology and Life Sciences
Subject code	610
Language	English
Publishing date	2011-10-01T00:00:00Z
Publisher	BioMed Central
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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