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Article: The Arsenal of Bioactive Molecules in the Skin Secretion of Urodele Amphibians.

Barros, Ana L A N / Hamed, Abdelaaty / Marani, Mariela / Moreira, Daniel C / Eaton, Peter / Plácido, Alexandra / Kato, Massuo J / Leite, José Roberto S A

Frontiers in pharmacology

2022 Volume 12, Page(s) 810821

Abstract: Urodele amphibians (∼768 spp.), salamanders and newts, are a rich source of molecules with bioactive properties, especially those isolated from their skin secretions. These include pharmacological attributes, such as antimicrobial, antioxidant, ... ...

Abstract	Urodele amphibians (∼768 spp.), salamanders and newts, are a rich source of molecules with bioactive properties, especially those isolated from their skin secretions. These include pharmacological attributes, such as antimicrobial, antioxidant, vasoactive, immune system modulation, and dermal wound healing activities. Considering the high demand for new compounds to guide the discovery of new drugs to treat conventional and novel diseases, this review summarizes the characteristics of molecules identified in the skin of urodele amphibians. We describe urodele-derived peptides and alkaloids, with emphasis on their biological activities, which can be considered new scaffolds for the pharmaceutical industry. Although much more attention has been given to anurans, bioactive molecules produced by urodeles have the potential to be used for biotechnological purposes and stand as viable alternatives for the development of therapeutic agents.
Language	English
Publishing date	2022-01-14
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2021.810821
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: The Peptide Salamandrin-I Modulates Components Involved in Pyroptosis and Induces Cell Death in Human Leukemia Cell Line HL-60.

Silva-Carvalho, Amandda Évelin / Oliveira, Nakaly Natiely de / Machado, Julia Viana Lafetá / Moreira, Daniel Carneiro / Brand, Guilherme Dotto / Leite, José Roberto S A / Plácido, Alexandra / Eaton, Peter / Saldanha-Araujo, Felipe

Pharmaceutics

2023 Volume 15, Issue 7

Abstract: Amphibian secretions have been extensively investigated for the production of bioactive molecules. Salamandrin-I is an antioxidant peptide, isolated from the skin secretion of the fire salamander, that has induced no toxicity in microglia or erythrocytes. ...

Abstract	Amphibian secretions have been extensively investigated for the production of bioactive molecules. Salamandrin-I is an antioxidant peptide, isolated from the skin secretion of the fire salamander, that has induced no toxicity in microglia or erythrocytes. Importantly, the administration of antioxidants may constitute an adequate therapeutic approach to cancer treatment. Here, with the purpose of better characterizing the therapeutic potential of salamandrin-I, we investigated whether this antioxidant peptide also exerts anticancer activity, using the human leukemia cell line HL-60 as a cancer model. Salamandrin-I treatment induced a significant reduction in HL-60 proliferation, which was accompanied by cell cycle arrest. Furthermore, the peptide-induced cell death showed a significant increase in the LDH release in HL-60 cells. The cellular toxicity exerted by salamandrin-I is possibly related to pyroptosis, since the HL-60 cells showed loss of mitochondrial membrane potential and hyperexpression of inflammasome components following the peptide treatment. This is the first demonstration of the anticancer potential of the salamandrin-I peptide. Such results are important, as they offer relevant insights into the field of cancer therapy and allow the design of future bioactive molecules using salamandrin-I as a template.
Language	English
Publishing date	2023-07-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527217-2
ISSN	1999-4923
ISSN	1999-4923
DOI	10.3390/pharmaceutics15071864
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Article ; Online: A convenient renewable surface plasmon resonance chip for relative quantification of genetically modified soybean in food and feed.

Plácido, Alexandra / Ferreira-da-Silva, Frederico / Leite, José Roberto S A / de-Los-Santos-Álvarez, Noemí / Delerue-Matos, Cristina

PloS one

2020 Volume 15, Issue 2, Page(s) e0229659

Abstract: The cultivation of genetically modified organisms (GMO) continues to expand worldwide. Still, many consumers express concerns about the use of GMO in food or feed, and many countries have legislated on labelling systems to indicate the presence of GMO in ...

Abstract	The cultivation of genetically modified organisms (GMO) continues to expand worldwide. Still, many consumers express concerns about the use of GMO in food or feed, and many countries have legislated on labelling systems to indicate the presence of GMO in commercial products. To deal with the increased number of GMO events and to address related regulations, alternative detection methods for GMO inspection are required. In this work, a genosensor based on Surface Plasmon Resonance under continuous flow was developed for the detection and quantification of a genetically modified soybean (event GTS 40-3-2). In a single chip, the simultaneous detection of the event-specific and the taxon-specific samples were achieved, whose detection limits were 20 pM and 16 pM, respectively. The reproducibility was 1.4%, which supports the use of the chip as a reliable and cost-effective alternative to other DNA-based techniques. The results indicate that the proposed method is a versatile tool for GMO quantification in food and feed samples.
MeSH term(s)	DNA, Plant/genetics ; Food, Genetically Modified/classification ; Oligonucleotide Array Sequence Analysis/methods ; Organisms, Genetically Modified/chemistry ; Organisms, Genetically Modified/genetics ; Plants, Genetically Modified/genetics ; Reproducibility of Results ; Glycine max/genetics ; Surface Plasmon Resonance/methods
Chemical Substances	DNA, Plant
Language	English
Publishing date	2020-02-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0229659
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Article: Antiviral Action against SARS-CoV-2 of a Synthetic Peptide Based on a Novel Defensin Present in the Transcriptome of the Fire Salamander (

Barros, Ana Luisa A N / Silva, Vladimir C / Ribeiro-Junior, Atvaldo F / Cardoso, Miguel G / Costa, Samuel R / Moraes, Carolina B / Barbosa, Cecília G / Coleone, Alex P / Simões, Rafael P / Cabral, Wanessa F / Falcão, Raul M / Vasconcelos, Andreanne G / Rocha, Jefferson A / Arcanjo, Daniel D R / Batagin-Neto, Augusto / Borges, Tatiana Karla S / Gonçalves, João / Brand, Guilherme D / Freitas-Junior, Lucio H G /

Eaton, Peter / Marani, Mariela / Kato, Massuo J / Plácido, Alexandra / Leite, José Roberto S A

Pharmaceutics

2024 Volume 16, Issue 2

Abstract: The potential emergence of zoonotic diseases has raised significant concerns, particularly in light of the recent pandemic, emphasizing the urgent need for scientific preparedness. The bioprospection and characterization of new molecules are ... ...

Abstract	The potential emergence of zoonotic diseases has raised significant concerns, particularly in light of the recent pandemic, emphasizing the urgent need for scientific preparedness. The bioprospection and characterization of new molecules are strategically relevant to the research and development of innovative drugs for viral and bacterial treatment and disease management. Amphibian species possess a diverse array of compounds, including antimicrobial peptides. This study identified the first bioactive peptide from
Language	English
Publishing date	2024-01-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527217-2
ISSN	1999-4923
ISSN	1999-4923
DOI	10.3390/pharmaceutics16020190
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Article ; Online: Electrochemical genoassays on gold-coated magnetic nanoparticles to quantify genetically modified organisms (GMOs) in food and feed as GMO percentage.

Plácido, Alexandra / Pereira, Clara / Guedes, Alexandra / Barroso, M Fátima / Miranda-Castro, Rebeca / de-Los-Santos-Álvarez, Noemí / Delerue-Matos, Cristina

Biosensors & bioelectronics

2018 Volume 110, Page(s) 147–154

Abstract: The integration of nanomaterials in the field of (bio)sensors has allowed developing strategies with improved analytical performance. In this work, ultrasmall core-shell ... ...

Abstract	The integration of nanomaterials in the field of (bio)sensors has allowed developing strategies with improved analytical performance. In this work, ultrasmall core-shell Fe
MeSH term(s)	Biosensing Techniques/methods ; DNA, Plant/genetics ; Electrochemical Techniques/methods ; Gold/chemistry ; Magnetite Nanoparticles/chemistry ; Magnetite Nanoparticles/ultrastructure ; Nucleic Acid Hybridization ; Plants, Genetically Modified/genetics ; Glycine max/genetics
Chemical Substances	DNA, Plant ; Magnetite Nanoparticles ; Gold (7440-57-5)
Language	English
Publishing date	2018-03-21
Publishing country	England
Document type	Journal Article
ZDB-ID	1011023-9
ISSN	1873-4235 ; 0956-5663
ISSN (online)	1873-4235
ISSN	0956-5663
DOI	10.1016/j.bios.2018.03.042
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Lycopene from Red Guava (

Alves, Maíra Bernardes / Vasconcelos, Andreanne Gomes / Silva de Carvalho, Amandda Évelin / Slompo, Robson Camilotti / Sá, Bruno Silva / Gonçalves, Maria Júlia Lima / Lima Moura, Liz Nayara Ribeiro da Costa / Brito, Ana Karolinne da Silva / França, José Vinícius de Sousa / Martins, Maria do Carmo de Carvalho E / Rizzo, Márcia Dos Santos / Soares, Susana / Bastos, Verónica / Saldanha de Araujo, Felipe / Mogharbel, Bassam Felipe / Carvalho, Katherine Athayde Teixeira de / Oliveira, Helena / Plácido, Alexandra / Arcanjo, Daniel Dias Rufino /

Barbosa, Eder Alves / Leite, José Roberto de Souza de Almeida

Pharmaceuticals (Basel, Switzerland)

2023 Volume 16, Issue 6

Abstract: Lycopene is a carotenoid with potential use in the treatment of chronic illnesses. Here, different formulations of lycopene were studied: lycopene-rich extract from red guava (LEG), purified lycopene from red guava (LPG) and a self-emulsifying drug ... ...

Abstract	Lycopene is a carotenoid with potential use in the treatment of chronic illnesses. Here, different formulations of lycopene were studied: lycopene-rich extract from red guava (LEG), purified lycopene from red guava (LPG) and a self-emulsifying drug delivery system loaded with LPG (nanoLPG). The effects of administering orally various doses of LEG to hypercholesterolemic hamsters were evaluated regarding the liver function of the animals. The cytotoxicity of LPG in Vero cells was analyzed by a crystal violet assay and by fluorescence microscopy. In addition, nanoLPG was employed in stability tests. LPG and nanoLPG were tested for their cytotoxic effect on human keratinocytes and antioxidant capacity on cells in an endothelial dysfunction model in an isolated rat aorta. Finally, the effect of different nanoLPG concentrations on the expression of immune-related genes (
Language	English
Publishing date	2023-06-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2193542-7
ISSN	1424-8247
ISSN	1424-8247
DOI	10.3390/ph16060905
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Article: Synthesis of novel sulfide-based cyclic peptidomimetic analogues to solonamides.

Brango-Vanegas, José / Martinho, Luan A / Bessa, Lucinda J / Vasconcelos, Andreanne G / Plácido, Alexandra / Pereira, Alex L / Leite, José R S A / Machado, Angelo H L

Beilstein journal of organic chemistry

2019 Volume 15, Page(s) 2544–2551

Abstract: Eight new sulfide-based cyclic peptidomimetic analogues of solonamides A and B have been synthesized via solid-phase peptide synthesis and ... ...

Abstract	Eight new sulfide-based cyclic peptidomimetic analogues of solonamides A and B have been synthesized via solid-phase peptide synthesis and S
Language	English
Publishing date	2019-10-25
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2192461-2
ISSN	1860-5397
ISSN	1860-5397
DOI	10.3762/bjoc.15.247
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Article: BR-bombesin: a novel bombesin-related peptide from the skin secretion of the Chaco tree frog (Boana raniceps) with physiological gastric effects

de Sousa, Nayara Alves / Marani, Mariela M. / Lopes, André Luís Fernandes / Silva, Emanuelle Morais / Barbosa, Eder Alves / Vasconcelos, Andreanne Gomes / Kuzniewski, Felipe T. B. / Lustosa, Suellen Sousa / Gomes, Karina Pereira / Colugnati, Diego Basile / Rocha, Jefferson A. / Santos, Lucianna Helene / Benquerer, Marcelo P. / Quelemes, Patrick / Véras, Leiz / Moreira, Daniel C. / Gadelha, Kalinne Kelly Lima / Magalhães, Pedro Jorge Caldas / Plácido, Alexandra /

Eaton, Peter / Nicolau, Lucas / Medeiros, Jand Venes R. / Leite, José R. S. A.

Amino acids. 2022 May, v. 54, no. 5

2022

Abstract: Bombesin mediates several biological activities in the gastrointestinal (GI) tract and central nervous system in mammals, including smooth muscle contraction, secretion of GI hormones and regulation of homeostatic mechanisms. Here, we report a novel ... ...

Abstract	Bombesin mediates several biological activities in the gastrointestinal (GI) tract and central nervous system in mammals, including smooth muscle contraction, secretion of GI hormones and regulation of homeostatic mechanisms. Here, we report a novel bombesin-like peptide isolated from Boana raniceps. Its amino acid sequence, GGNQWAIGHFM-NH₂, was identified and structurally confirmed by HPLC, MS/MS and 454-pyrosequencing; the peptide was named BR-bombesin. The effect of BR-bombesin on smooth muscle contraction was assessed in ileum and esophagus, and its anti-secretory activity was investigated in the stomach. BR-bombesin exerted significant contractile activity with a concentration–response curve similar to that of commercially available bombesin in ileum strips of Wistar rats. In esophageal strips, BR-bombesin acted as an agonist, as many other bombesin-related peptides act, although with different behavior compared to the muscarinic agonist carbachol. Moreover, BR-bombesin inhibited stomach secretion by approximately 50% compared to the untreated control group. This novel peptide has 80% and 70% similarity with the 10-residue C-terminal domain of human neuromedin B (NMB) and human gastrin releasing peptide (GRP10), respectively. Molecular docking analysis revealed that the GRP receptor had a binding energy equal to − 7.3 kcal.mol⁻¹ and − 8.5 kcal.mol⁻¹ when interacting with bombesin and BR-bombesin, respectively. Taken together, our data open an avenue to investigate BR-bombesin in disorders that involve gastrointestinal tract motility and acid gastric secretion.
Keywords	Hylidae ; agonists ; amino acid sequences ; bombesin ; carbachol ; central nervous system ; digestive tract ; energy ; esophagus ; gastrins ; high-throughput nucleotide sequencing ; humans ; ileum ; muscle contraction ; smooth muscle ; stomach
Language	English
Dates of publication	2022-05
Size	p. 733-747.
Publishing place	Springer Vienna
Document type	Article
ZDB-ID	1121341-3
ISSN	1438-2199 ; 0939-4451
ISSN (online)	1438-2199
ISSN	0939-4451
DOI	10.1007/s00726-021-03114-4
Database	NAL-Catalogue (AGRICOLA)

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Zs.A 3490: Show issues

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Article: Mechanistic Insights into the Leishmanicidal and Bactericidal Activities of Batroxicidin, a Cathelicidin-Related Peptide from a South American Viper (Bothrops atrox)

Journal of natural products. 2021 June 02, v. 84, no. 6

2021

Abstract: Snake venoms are important sources of bioactive molecules, including those with antiparasitic activity. Cathelicidins form a class of such molecules, which are produced by a variety of organisms. Batroxicidin (BatxC) is a cathelicidin found in the venom ... ...

Abstract	Snake venoms are important sources of bioactive molecules, including those with antiparasitic activity. Cathelicidins form a class of such molecules, which are produced by a variety of organisms. Batroxicidin (BatxC) is a cathelicidin found in the venom of the common lancehead (Bothrops atrox). In the present work, BatxC and two synthetic analogues, BatxC(C-2.15Phe) and BatxC(C-2.14Phe)des-Phe1, were assessed for their microbicidal activity. All three peptides showed a broad-spectrum activity on Gram-positive and -negative bacteria, as well as promastigote and amastigote forms of Leishmania (Leishmania) amazonensis. Circular dichroism (CD) and nuclear magnetic resonance (NMR) data indicated that the three peptides changed their structure upon interaction with membranes. Biomimetic membrane model studies demonstrated that the peptides exert a permeabilization effect in prokaryotic membranes, leading to cell morphology distortion, which was confirmed by atomic force microscopy (AFM). The molecules considered in this work exhibited bactericidal and leishmanicidal activity at low concentrations, with the AFM data suggesting membrane pore formation as their mechanism of action. These peptides stand as valuable prototype drugs to be further investigated and eventually used to treat bacterial and protozoal infections.
Keywords	Bothrops atrox ; antileishmanial properties ; artificial membranes ; atomic force microscopy ; cathelicidins ; cell structures ; circular dichroism spectroscopy ; mechanism of action ; nuclear magnetic resonance spectroscopy ; permeability ; prototypes
Language	English
Dates of publication	2021-0602
Size	p. 1787-1798.
Publishing place	American Chemical Society and American Society of Pharmacognosy
Document type	Article
ZDB-ID	304325-3
ISSN	1520-6025 ; 0163-3864
ISSN (online)	1520-6025
ISSN	0163-3864
DOI	10.1021/acs.jnatprod.1c00153
Database	NAL-Catalogue (AGRICOLA)

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Zs.A 1144: Show issues

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Article: Determination of Methiocarb and Its Degradation Products, Methiocarb Sulfoxide and Methiocarb Sulfone, in Bananas Using QuEChERS Extraction

Plácido, Alexandra / Paíga, Paula / Lopes, David H / Correia, Manuela / Delerue-Matos, Cristina

Journal of agricultural and food chemistry. 2013 Jan. 16, v. 61, no. 2

2013

Abstract: The present work describes the development of an analytical method for the determination of methiocarb and its degradation products (methiocarb sulfoxide and methiocarb sulfone) in banana samples, using the QuEChERS (quick, easy, cheap, effective, rugged, ...

Abstract	The present work describes the development of an analytical method for the determination of methiocarb and its degradation products (methiocarb sulfoxide and methiocarb sulfone) in banana samples, using the QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure followed by liquid chromatography coupled to photodiode array detector (LC-PAD). Calibration curves were linear in the range of 0.5-10 mg L-1 for all compounds studied. The average recoveries, measured at 0.1 mg kg-1 wet weight, were 92.0 (RSD = 1.8%, n = 3), 84.0 (RSD = 3.9%, n = 3), and 95.2% (RSD = 1.9%, n = 3) for methiocarb sulfoxide, methiocarb sulfone, and methiocarb, respectively. Banana samples treated with methiocarb were collected from an experimental field. The developed method was applied to the analysis of 24 samples (peel and pulp) and to 5 banana pulp samples. Generally, the highest levels were found for methiocarb sulfoxide and methiocarb. Methiocarb sulfone levels were below the limit of quantification, except in one sample (not detected).
Keywords	bananas ; field experimentation ; liquid chromatography ; methiocarb ; pulp
Language	English
Size	p. 325-331.
Document type	Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/jf304027s
Database	NAL-Catalogue (AGRICOLA)

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