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Article ; Online: Transcriptomic Study on Human Skin Samples: Identification of Two Subclasses of Actinic Keratoses.

Dubois-Pot-Schneider, Hélène / Khairallah, Grégoire / Brzenczek, Cyril / Plénat, François / Marchal, Frédéric / Amouroux, Marine

International journal of molecular sciences

2023 Volume 24, Issue 6

Abstract: Actinic keratoses (AKs) are sun-damaged skin areas that affect 20% of the European adult population and more than 50% of people aged 70 years and over. There are currently no clinical or histological features allowing us to identify to which clinical ... ...

Abstract	Actinic keratoses (AKs) are sun-damaged skin areas that affect 20% of the European adult population and more than 50% of people aged 70 years and over. There are currently no clinical or histological features allowing us to identify to which clinical class (i.e., regression or progression) an AK belongs. A transcriptomic approach seems to be a robust tool for AK characterization, but there is a need for additional studies, including more patients and elucidating the molecular signature of an AK. In this context, the present study, including the largest number of patients to date, is the first aiming at identifying biological features to objectively distinguish different AK signatures. We highlight two distinct molecular profiles: AKs featuring a molecular profile similar to squamous cell carcinomas (SCCs), which are called "lesional AKs" (AK_Ls), and AKs featuring a molecular profile similar to normal skin tissue, which are called "non-lesional AKs" (AK_NLs). The molecular profiles of both AK subclasses were studied, and 316 differentially expressed genes (DEGs) were identified between the two classes. The 103 upregulated genes in AK_L were related to the inflammatory response. Interestingly, downregulated genes were associated with keratinization. Finally, based on a connectivity map approach, our data highlight that the VEGF pathway could be a promising therapeutic target for high-risk lesions.
MeSH term(s)	Adult ; Humans ; Aged ; Aged, 80 and over ; Keratosis, Actinic/genetics ; Keratosis, Actinic/pathology ; Transcriptome ; Skin Neoplasms/pathology ; Skin/pathology ; Carcinoma, Squamous Cell/pathology
Language	English
Publishing date	2023-03-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24065937
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Article ; Online: Aggressive bone marrow metastatic medullary thyroid carcinoma.

Lesesve, Jean-Francois / Plénat, François

Blood

2014 Volume 123, Issue 17, Page(s) 2603

MeSH term(s)	Bone Marrow/pathology ; Calcitonin/metabolism ; Carcinoembryonic Antigen/metabolism ; Carcinoma/diagnosis ; Carcinoma/mortality ; Carcinoma/pathology ; Fatal Outcome ; Female ; Humans ; Middle Aged ; Multiple Endocrine Neoplasia/diagnosis ; Multiple Endocrine Neoplasia/mortality ; Multiple Endocrine Neoplasia/pathology ; Neoplasm Metastasis ; Thyroid Neoplasms/diagnosis ; Thyroid Neoplasms/mortality ; Thyroid Neoplasms/pathology
Chemical Substances	Carcinoembryonic Antigen ; Calcitonin (9007-12-9)
Language	English
Publishing date	2014-05-21
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	80069-7
ISSN	1528-0020 ; 0006-4971
ISSN (online)	1528-0020
ISSN	0006-4971
DOI	10.1182/blood-2014-01-550889
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Uh III Zs.140: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
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Article ; Online: The role of platelets and von Willebrand factor in the procoagulant phenotype of inflammatory bowel disease.

Schellenberg, Célia / Lagrange, Jérémy / Ahmed, Muhammad Usman / Arnone, Djésia / Campoli, Philippe / Louis, Huguette / Touly, Nina / Caron, Bénédicte / Plénat, François / Perrin, Julien / Lenting, Peter J / Regnault, Véronique / Lacolley, Patrick / Denis, Cécile V / Peyrin-Biroulet, Laurent

Journal of Crohn's & colitis

2023

Abstract: Objective: Although the risk for thrombosis is well documented for inflammatory bowel disease (IBD) patients, the underlying pathological mechanism seems to be different from other thrombotic conditions. Deciphering the actors responsible for the ... ...

Abstract	Objective: Although the risk for thrombosis is well documented for inflammatory bowel disease (IBD) patients, the underlying pathological mechanism seems to be different from other thrombotic conditions. Deciphering the actors responsible for the increased risk of thrombosis in IBD would help to improve management of this frequent complication. Design: We studied the interplay between platelets, coagulation, and von Willebrand factor (VWF) in 193 IBD patients and in experimental models (acute and chronic) of colitis in wild-type and VWF-deficient mice. Results: We found a platelet-dependent increase in thrombin generation in IBD patients and in our mouse model of colitis. Agglutinated platelets were present in the blood of patients and mice. Interestingly, we observed not only a significant increase in total VWF antigen, but we were able to detect the presence of active VWF (VWF in its platelet-binding conformation; 3.2±2.7µg/ml) in the plasma of 30% of all IBD patients. In healthy controls, active VWF levels were below 0.3µg/ml. This led us to further explore experimental colitis in VWF-deficient mice and we observed that these mice were protected against the procoagulant state triggered by the colitis. Unexpectedly, these mice also manifested a significant worsening of colitis severity both in acute and chronic models. Conclusion: Platelets and VWF (including its active form) appear to be central players in the procoagulant phenotype in IBD. We observed that the role of VWF in hemostasis differs from its role in colic tissue healing, potentially opening new therapeutic avenues for a life-threatening complication in IBD patients.
Language	English
Publishing date	2023-11-27
Publishing country	England
Document type	Journal Article
ZDB-ID	2390120-2
ISSN	1876-4479 ; 1873-9946
ISSN (online)	1876-4479
ISSN	1873-9946
DOI	10.1093/ecco-jcc/jjad198
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Zs.A 6634: Show issues

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Article ; Online: PTEN expression is involved in the invasive properties of HNSCC: a key protein to consider in locoregional recurrence.

Mriouah, Jihane / Boura, Cédric / Gargouri, Myriem / Plénat, François / Faivre, Béatrice

International journal of oncology

2013 Volume 44, Issue 3, Page(s) 709–716

Abstract: Specific phenotypic effects of PTEN in head and neck squamous cell carcinoma (HNSCC) remain poorly defined without a direct causal connection between the loss of PTEN function and the progression of cancer. Here, we describe a potential role for PTEN in ... ...

Abstract	Specific phenotypic effects of PTEN in head and neck squamous cell carcinoma (HNSCC) remain poorly defined without a direct causal connection between the loss of PTEN function and the progression of cancer. Here, we describe a potential role for PTEN in cancer progression. Using an shRNA targeting PTEN in HNSCC cells, we show that the loss of PTEN expression is associated with a decrease of cell adhesion, a reduction in E-cadherin expression while cell migration is promoted. Together with the tissue organization and molecular markers expressed in tumors derived from shPTEN cells in vivo, this study indicates that HNSCC cells deficient in PTEN expression undergo an epithelial‑mesenchymal transition (EMT). Additionally, our results suggest that both the low levels of expression and subcellular localization of PTEN are involved in the EMT phenotype, and ultimately in possible locoregional reccurences. We hypothesize that the loss of PTEN expression as well as the subcellular localization could be of interest as a predictive marker of recurrence in HNSCC.
MeSH term(s)	Cadherins/genetics ; Carcinoma, Squamous Cell/genetics ; Carcinoma, Squamous Cell/pathology ; Cell Line, Tumor ; Cell Movement/genetics ; Cell Proliferation ; Epithelial-Mesenchymal Transition/genetics ; Gene Expression Regulation, Neoplastic ; Head and Neck Neoplasms/genetics ; Head and Neck Neoplasms/pathology ; Humans ; Neoplasm Recurrence, Local/genetics ; Neoplasm Recurrence, Local/pathology ; PTEN Phosphohydrolase/biosynthesis ; PTEN Phosphohydrolase/genetics ; RNA, Small Interfering ; Squamous Cell Carcinoma of Head and Neck
Chemical Substances	Cadherins ; RNA, Small Interfering ; PTEN Phosphohydrolase (EC 3.1.3.67) ; PTEN protein, human (EC 3.1.3.67)
Language	English
Publishing date	2013-12-19
Publishing country	Greece
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1154403-x
ISSN	1791-2423 ; 1019-6439
ISSN (online)	1791-2423
ISSN	1019-6439
DOI	10.3892/ijo.2013.2219
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Zs.A 3690: Show issues

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Article: Les marqueurs de vitalité des blessures en pathologie médicolégale.

Gauchotte, Guillaume / Martrille, Laurent / Plénat, François / Vignaud, Jean-Michel

Annales de pathologie

2013 Volume 33, Issue 2, Page(s) 93–101

Abstract: Skin wounds datation is one of the most challenging problems in forensic pathology. The vitality of a recent wound cannot be affirmed when no inflammatory cell is visible. There are in the literature numerous studies about wound vitality, looking for ... ...

Title translation	The markers of wound vitality in forensic pathology.
Abstract	Skin wounds datation is one of the most challenging problems in forensic pathology. The vitality of a recent wound cannot be affirmed when no inflammatory cell is visible. There are in the literature numerous studies about wound vitality, looking for markers involved in coagulation or inflammation, using various methods such as enzymology, molecular biology or immunohistochemistry. In this update, we first introduce some methodological principles to respect. Then, we review the main studies available in the literature. We insist on immunohistochemistry, which seems to be the more valuable method, given its easiness to perform and the possibility to analyze the localization of the molecules of interest. Some markers are promising, such as TNFα, IL-6, IL-1β, TGFα or TGFβ1. Before using them in daily practice, these first results need to be confirmed with other studies, driven by independent teams and integrating multiple controls. Most notably, the antibodies have to be tested in numerous post-mortem wounds. Indeed, there is a critical risk of overexpression in post-mortem wounds, and some interesting markers have been secondary invalidated because of post-mortem false positivity (e.g. fibronectin, P-selectin). Finally, optimal sensibility and specificity values would be probably reached by combining several markers, validated with large groups of pre- and post-mortem wounds.
MeSH term(s)	Biomarkers/analysis ; Blood Coagulation ; Cell Adhesion Molecules/analysis ; Forensic Pathology/methods ; Hemostasis ; Humans ; Immunohistochemistry ; Inflammation/metabolism ; Inflammation/pathology ; Interleukin-1beta/analysis ; Interleukin-6/analysis ; Skin/chemistry ; Skin/pathology ; Transforming Growth Factor alpha/analysis ; Transforming Growth Factor beta1/analysis ; Tumor Necrosis Factor-alpha/analysis ; Wounds and Injuries/pathology ; Wounds and Injuries/physiopathology
Chemical Substances	Biomarkers ; Cell Adhesion Molecules ; Interleukin-1beta ; Interleukin-6 ; Transforming Growth Factor alpha ; Transforming Growth Factor beta1 ; Tumor Necrosis Factor-alpha
Language	French
Publishing date	2013-04
Publishing country	France
Document type	English Abstract ; Journal Article ; Review
ZDB-ID	225720-8
ISSN	0242-6498
ISSN	0242-6498
DOI	10.1016/j.annpat.2013.02.006
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Zs.A 1652: Show issues

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Article ; Online: The VWF/LRP4/αVβ3-axis represents a novel pathway regulating proliferation of human vascular smooth muscle cells.

Lagrange, Jérémy / Worou, Morel E / Michel, Jean-Baptiste / Raoul, Alexandre / Didelot, Mélusine / Muczynski, Vincent / Legendre, Paulette / Plénat, François / Gauchotte, Guillaume / Lourenco-Rodrigues, Marc-Damien / Christophe, Olivier D / Lenting, Peter J / Lacolley, Patrick / Denis, Cécile V / Regnault, Véronique

Cardiovascular research

2021 Volume 118, Issue 2, Page(s) 622–637

Abstract: Aims: Von Willebrand factor (VWF) is a plasma glycoprotein involved in primary haemostasis, while also having additional roles beyond haemostasis namely in cancer, inflammation, angiogenesis, and potentially in vascular smooth muscle cell (VSMC) ... ...

Abstract	Aims: Von Willebrand factor (VWF) is a plasma glycoprotein involved in primary haemostasis, while also having additional roles beyond haemostasis namely in cancer, inflammation, angiogenesis, and potentially in vascular smooth muscle cell (VSMC) proliferation. Here, we addressed how VWF modulates VSMC proliferation and investigated the underlying molecular pathways and the in vivo pathophysiological relevance. Methods and results: VWF induced proliferation of human aortic VSMCs and also promoted VSMC migration. Treatment of cells with a siRNA against αv integrin or the RGT-peptide blocking αvβ3 signalling abolished proliferation. However, VWF did not bind to αvβ3 on VSMCs through its RGD-motif. Rather, we identified the VWF A2 domain as the region mediating binding to the cells. We hypothesized the involvement of a member of the LDL-related receptor protein (LRP) family due to their known ability to act as co-receptors. Using the universal LRP-inhibitor receptor-associated protein, we confirmed LRP-mediated VSMC proliferation. siRNA experiments and confocal fluorescence microscopy identified LRP4 as the VWF-counterreceptor on VSMCs. Also co-localization between αvβ3 and LRP4 was observed via proximity ligation analysis and immuno-precipitation experiments. The pathophysiological relevance of our data was supported by VWF-deficient mice having significantly reduced hyperplasia in carotid artery ligation and artery femoral denudation models. In wild-type mice, infiltration of VWF in intimal regions enriched in proliferating VSMCs was found. Interestingly, also analysis of human atherosclerotic lesions showed abundant VWF accumulation in VSMC-proliferating rich intimal areas. Conclusion: VWF mediates VSMC proliferation through a mechanism involving A2 domain binding to the LRP4 receptor and integrin αvβ3 signalling. Our findings provide new insights into the mechanisms that drive physiological repair and pathological hyperplasia of the arterial vessel wall. In addition, the VWF/LRP4-axis may represent a novel therapeutic target to modulate VSMC proliferation.
MeSH term(s)	Animals ; Atherosclerosis/genetics ; Atherosclerosis/metabolism ; Atherosclerosis/pathology ; Carotid Artery Injuries/genetics ; Carotid Artery Injuries/metabolism ; Carotid Artery Injuries/pathology ; Cell Movement ; Cell Proliferation ; Cells, Cultured ; Hyperplasia ; Integrin alphaVbeta3/genetics ; Integrin alphaVbeta3/metabolism ; LDL-Receptor Related Proteins/genetics ; LDL-Receptor Related Proteins/metabolism ; Male ; Mice, Inbred C57BL ; Mice, Knockout ; Muscle, Smooth, Vascular/injuries ; Muscle, Smooth, Vascular/metabolism ; Muscle, Smooth, Vascular/pathology ; Myocytes, Smooth Muscle/metabolism ; Myocytes, Smooth Muscle/pathology ; Neointima ; Plaque, Atherosclerotic ; Signal Transduction ; Vascular System Injuries/genetics ; Vascular System Injuries/metabolism ; Vascular System Injuries/pathology ; von Willebrand Factor/genetics ; von Willebrand Factor/metabolism ; Mice
Chemical Substances	Integrin alphaVbeta3 ; LDL-Receptor Related Proteins ; LRP4 protein, human ; von Willebrand Factor
Language	English
Publishing date	2021-02-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80340-6
ISSN	1755-3245 ; 0008-6363
ISSN (online)	1755-3245
ISSN	0008-6363
DOI	10.1093/cvr/cvab042
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Zs.A 565: Show issues

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Article ; Online: A new algorithm for a better characterization and timing of the anti-VEGF vascular effect named "normalization".

El Alaoui-Lasmaili, Karima / Djermoune, El-Hadi / Tylcz, Jean-Baptiste / Meng, Dominique / Plénat, François / Thomas, Noémie / Faivre, Béatrice

Angiogenesis

2017 Volume 20, Issue 1, Page(s) 149–162

Abstract: Antiangiogenics are widely used in cancer treatment in combination with chemotherapy and radiotherapy for their vascular effects. Antiangiogenics are supposed to induce morphological and functional changes in the chaotic tumor vasculature that would help ...

Abstract	Antiangiogenics are widely used in cancer treatment in combination with chemotherapy and radiotherapy for their vascular effects. Antiangiogenics are supposed to induce morphological and functional changes in the chaotic tumor vasculature that would help enhance the therapeutic efficacy of chemotherapy and radiotherapy through the amelioration of the drug delivery or the oxygenation in the tumor, respectively. However, finding the best treatment sequence is not an easy task to achieve and no consensus has yet been established because of the lack of knowledge regarding when and for how long the vascular network is ameliorated. The aim of this work was to develop a dedicated image processing algorithm able to analyze the vascular structures on optical microscopy images of the vascular network and to follow its fine modifications in vivo, over time. We applied this algorithm to follow the evolution of the vascular parameters (vascularized tissue surface, branches, sprouts and length), in response or not to anti-VEGF therapy (10 mg/kg/day) and determine precisely whether there is really a vascular "normalization" with anti-VEGF therapy in comparison with the parameters extracted from healthy vascular networks. We found that for this determination, the choice of region of interest to analyze is critical as it is important to compare only microcirculation areas and avoid areas with arteriole-venule-capillary hierarchy. The algorithm analysis allowed us to define a vascular "normalization" in treated tumors, between 8 and 12 days of bevacizumab treatment that was confirmed by standard immunohistochemical analysis, microvascular permeability assessment and immunohistological blood perfusion assessment.
Language	English
Publishing date	2017-02
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1484717-6
ISSN	1573-7209 ; 0969-6970
ISSN (online)	1573-7209
ISSN	0969-6970
DOI	10.1007/s10456-016-9536-3
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Zs.A 5094: Show issues

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Article ; Online: Radiosensitizing properties of bortezomib depend on therapeutic schedule.

Labussière, Marianne / Pinel, Sophie / Vandamme, Marc / Plénat, François / Chastagner, Pascal

International journal of radiation oncology, biology, physics

2011 Volume 79, Issue 3, Page(s) 892–900

Abstract: Purpose: To investigate the influence of the bortezomib (BTZ) on malignant glioma radiosensitivity in two xenograft models.: Methods and materials: For TCG3 and U87 models, we evaluated the antitumor activity of BTZ, radiotherapy, and BTZ plus ... ...

Abstract	Purpose: To investigate the influence of the bortezomib (BTZ) on malignant glioma radiosensitivity in two xenograft models. Methods and materials: For TCG3 and U87 models, we evaluated the antitumor activity of BTZ, radiotherapy, and BTZ plus radiothearapy according to two therapeutic schedules: a "nonfractionated" schedule corresponding to a single dose of treatment per week, and a "fractionated" schedule corresponding to the same weekly dose divided into 5 fractions. Treatments influence on proliferation and apoptosis indexes, cell cycle distribution, and nuclear factor-κB pathway were explored. Results: The radiosensitizing properties of BTZ observed with the nonfractionated schedule were lost with the fractionated schedule. Bortezomib-mediated radiosensitization was associated with an increased apoptosis response and major changes in cell proliferation, but the nuclear factor-κB pathway was not involved. Most of the cellular effects induced by BTZ when tumors received a single irradiation were cancelled out if radiotherapy was fractionated. Conclusion: The influence of BTZ on glioma radiosensitivity seems to depend on the treatment fractionation schedule, emphasizing the need to clarify the mechanisms underlying BTZ's radiosensitizing effects before further clinical trials are initiated.
MeSH term(s)	Animals ; Apoptosis/drug effects ; Boronic Acids/pharmacology ; Bortezomib ; Cell Cycle/drug effects ; Cell Proliferation/drug effects ; Combined Modality Therapy/methods ; Dose Fractionation ; Drug Administration Schedule ; Female ; Glioma/metabolism ; Glioma/pathology ; Glioma/radiotherapy ; Mice ; Mice, Nude ; NF-kappa B/metabolism ; Pyrazines/pharmacology ; Radiation Tolerance/drug effects ; Radiation-Sensitizing Agents/pharmacology ; Random Allocation ; Xenograft Model Antitumor Assays
Chemical Substances	Boronic Acids ; NF-kappa B ; Pyrazines ; Radiation-Sensitizing Agents ; Bortezomib (69G8BD63PP)
Language	English
Publishing date	2011-03-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	197614-x
ISSN	1879-355X ; 0360-3016
ISSN (online)	1879-355X
ISSN	0360-3016
DOI	10.1016/j.ijrobp.2010.09.051
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Zs.A 1218: Show issues

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Article: Clear cell adenocarcinoma: a rare variant of primary colonic tumour.

Bressenot, Aude / Marcon, Nathalie / Montagne, Karine / Plénat, François

International journal of colorectal disease

2008 Volume 23, Issue 1, Page(s) 137–138

MeSH term(s)	Adenocarcinoma, Clear Cell/pathology ; Adenocarcinoma, Clear Cell/surgery ; Aged, 80 and over ; Anastomosis, Surgical ; Colectomy ; Colonic Neoplasms/pathology ; Colonic Neoplasms/surgery ; Colonoscopy ; Female ; Humans ; Immunohistochemistry ; Treatment Outcome
Language	English
Publishing date	2008-01
Publishing country	Germany
Document type	Case Reports ; Letter
ZDB-ID	84975-3
ISSN	1432-1262 ; 0179-1958
ISSN (online)	1432-1262
ISSN	0179-1958
DOI	10.1007/s00384-007-0291-1
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Zs.A 2121: Show issues

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Article ; Online: An alkylphenol mix promotes seminoma derived cell proliferation through an ERalpha36-mediated mechanism.

Ajj, Hussein / Chesnel, Amand / Pinel, Sophie / Plenat, François / Flament, Stephane / Dumond, Helene

PloS one

2013 Volume 8, Issue 4, Page(s) e61758

Abstract: Long chain alkylphenols are man-made compounds still present in industrial and agricultural processes. Their main use is domestic and they are widespread in household products, cleansers and cosmetics, leading to a global environmental and human ... ...

Abstract	Long chain alkylphenols are man-made compounds still present in industrial and agricultural processes. Their main use is domestic and they are widespread in household products, cleansers and cosmetics, leading to a global environmental and human contamination. These molecules are known to exert estrogen-like activities through binding to classical estrogen receptors. In vitro, they can also interact with the G-protein coupled estrogen receptor. Testicular germ cell tumor etiology and progression are proposed to be stimulated by lifelong estrogeno-mimetic exposure. We studied the transduction signaling pathways through which an alkyphenol mixture triggers testicular cancer cell proliferation in vitro and in vivo. Proliferation assays were monitored after exposure to a realistic mixture of 4-tert-octylphenol and 4-nonylphenol of either TCam-2 seminoma derived cells, NT2/D1 embryonal carcinoma cells or testis tumor in xenografted nude mice. Specific pharmacological inhibitors and gene-silencing strategies were used in TCam-2 cells in order to demonstrate that the alkylphenol mix triggers CREB-phosphorylation through a rapid, ERα36-PI3kinase non genomic pathway. Microarray analysis of the mixture target genes revealed that this pathway can modulate the expression of the DNA-methyltransferase-3 (Dnmt3) gene family which is involved in DNA methylation control. Our results highlight a key role for ERα36 in alkylphenol non genomic signaling in testicular germ cell tumors. Hence, ERα36-dependent control of the epigenetic status opens the way for the understanding of the link between endocrine disruptor exposure and the burden of hormone sensitive cancers.
MeSH term(s)	Androstadienes/pharmacology ; Animals ; Carcinogens, Environmental/pharmacology ; Carcinoma/genetics ; Carcinoma/metabolism ; Carcinoma/pathology ; Cell Proliferation/drug effects ; Cyclic AMP Response Element-Binding Protein/genetics ; Cyclic AMP Response Element-Binding Protein/metabolism ; DNA (Cytosine-5-)-Methyltransferases/genetics ; DNA (Cytosine-5-)-Methyltransferases/metabolism ; Epigenesis, Genetic/drug effects ; Estrogen Receptor alpha/genetics ; Estrogen Receptor alpha/metabolism ; Gene Expression Regulation, Neoplastic/drug effects ; Male ; Mice ; Mice, Nude ; Neoplasm Transplantation ; Phenols/pharmacology ; Phosphatidylinositol 3-Kinases/genetics ; Phosphatidylinositol 3-Kinases/metabolism ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; Seminoma/genetics ; Seminoma/metabolism ; Seminoma/pathology ; Signal Transduction ; Testicular Neoplasms/genetics ; Testicular Neoplasms/metabolism ; Testicular Neoplasms/pathology ; Wortmannin
Chemical Substances	Androstadienes ; Carcinogens, Environmental ; Creb1 protein, mouse ; Cyclic AMP Response Element-Binding Protein ; Estrogen Receptor alpha ; Phenols ; Protein Isoforms ; DNA (Cytosine-5-)-Methyltransferases (EC 2.1.1.37) ; DNA methyltransferase 3A (EC 2.1.1.37) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; 4-nonylphenol (I03GBV4WEL) ; 4-tert-octylphenol (IOY9FVU3J3) ; Wortmannin (XVA4O219QW)
Language	English
Publishing date	2013-04-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0061758
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