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  1. Article ; Online: Tablet-assisted objective structured spotter practical examination (TOSSPE): Advantages of an innovative anatomy spotter examination method for medical student assessment.

    Polak, Katarzyna / Gielecki, Jerzy Stanisław / Żurada, Anna

    Anatomical sciences education

    2022  Volume 15, Issue 6, Page(s) 1060–1073

    Abstract: The affordances of technology-based assessments, like the objectively structured practical examination, have become an integral part of gross anatomy courses. The Department of Anatomy Faculty of Medicine at the University of Warmia and Mazury developed ... ...

    Abstract The affordances of technology-based assessments, like the objectively structured practical examination, have become an integral part of gross anatomy courses. The Department of Anatomy Faculty of Medicine at the University of Warmia and Mazury developed and introduced an application for tablet devices which has been implemented in student examinations and assessments, called the tablet-assisted objective structured spotter practical examination. It was created to simplify the educational process and to build a rich learning environment, facilitating deep learning for students through examination and feedback data. The method consists of cadaver stations with traditional corresponding pin spotters in an expanded tablet application. It not only provides instant feedback on various observations of teaching-learning skills but has also positively affected the entire process of education. The method provides an unbiased evaluation of knowledge and understanding of the anatomy course, ensuring objectivity and standardization. The current study was performed on a total of 608 first-year medical students in Polish and English divisions and focused on the observed advantages since the new method was introduced. Outcomes indicate that after the implementation of the method for both the Polish and English divisions' first-year medical students, the mean score of examinations significantly increased compared to other teaching-learning methods. The study highlights that students were excited about the implementation of the new method and identified its many benefits. It is recognized that technological development and the digital environment offer a range of opportunities and added value versus traditional assessment activities, methods, and processes.
    MeSH term(s) Humans ; Students, Medical ; Education, Medical, Undergraduate/methods ; Anatomy/education ; Educational Measurement/methods ; Curriculum ; Surveys and Questionnaires
    Language English
    Publishing date 2022-01-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2483491-9
    ISSN 1935-9780 ; 1935-9772
    ISSN (online) 1935-9780
    ISSN 1935-9772
    DOI 10.1002/ase.2131
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: The multifaceted view of heart problem in Duchenne muscular dystrophy

    Florczyk-Soluch, Urszula / Polak, Katarzyna / Dulak, Józef

    Cellular and molecular life sciences. 2021 July, v. 78, no. 14

    2021  

    Abstract: Dystrophin is a large protein serving as local scaffolding repetitively bridging cytoskeleton and the outside of striated muscle cell. As such dystrophin is a critical brick primarily in dystrophin-associated protein complex (DAGC) and in a larger ... ...

    Abstract Dystrophin is a large protein serving as local scaffolding repetitively bridging cytoskeleton and the outside of striated muscle cell. As such dystrophin is a critical brick primarily in dystrophin-associated protein complex (DAGC) and in a larger submembranous unit, costamere. Accordingly, the lack of functional dystrophin laying at the root of Duchenne muscular dystrophy (DMD) drives sarcolemma instability. From this point on, the cascade inevitably leading to the death of myocyte begins. In cardiomyocytes, intracellular calcium overload and related mitochondrial-mediated cell death mainly contribute to myocardial dysfunction and dilation while other protein dysregulation and/or mislocalization may affect electrical conduction system and favor arrhythmogenesis. Although clinically DMD manifests as progressive muscle weakness and skeletal muscle symptoms define characteristic of DMD, it is the heart problem the biggest challenge that most often develop in the form of dilated cardiomyopathy (DCM). Current standards of treatment and recent progress in respiratory care, introduced in most settings in the 1990s, have improved quality of life and median life expectancy to 4th decade of patient’s age. At the same time, cardiac causes of death related to DMD increases. Despite preventive and palliative cardiac treatments available, the prognoses remain poor. Direct therapeutic targeting of dystrophin deficiency is critical, however, hindered by the large size of the dystrophin cDNA and/or stochastic, often extensive genetic changes in DMD gene. The correlation between cardiac involvement and mutations affecting specific dystrophin isoforms, may provide a mutation-specific cardiac management and novel therapeutic approaches for patients with CM. Nonetheless, the successful cardiac treatment poses a big challenge and may require combined therapy to combat dystrophin deficiency and its after-effects (critical in DMD pathogenesis). This review locates the multifaceted heart problem in the course of DMD, balancing the insights into basic science, translational efforts and clinical manifestation of dystrophic heart disease.
    Keywords bricks ; calcium ; cardiomyocytes ; cardiomyopathy ; cell death ; cytoskeleton ; death ; dystrophin ; electrical conductivity ; genes ; longevity ; muscles ; muscular dystrophy ; pathogenesis ; patients ; quality of life ; skeletal muscle ; striated muscle ; therapeutics
    Language English
    Dates of publication 2021-07
    Size p. 5447-5468.
    Publishing place Springer International Publishing
    Document type Article
    Note Review
    ZDB-ID 1358415-7
    ISSN 1420-9071 ; 1420-682X
    ISSN (online) 1420-9071
    ISSN 1420-682X
    DOI 10.1007/s00018-021-03862-2
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: The multifaceted view of heart problem in Duchenne muscular dystrophy.

    Florczyk-Soluch, Urszula / Polak, Katarzyna / Dulak, Józef

    Cellular and molecular life sciences : CMLS

    2021  Volume 78, Issue 14, Page(s) 5447–5468

    Abstract: Dystrophin is a large protein serving as local scaffolding repetitively bridging cytoskeleton and the outside of striated muscle cell. As such dystrophin is a critical brick primarily in dystrophin-associated protein complex (DAGC) and in a larger ... ...

    Abstract Dystrophin is a large protein serving as local scaffolding repetitively bridging cytoskeleton and the outside of striated muscle cell. As such dystrophin is a critical brick primarily in dystrophin-associated protein complex (DAGC) and in a larger submembranous unit, costamere. Accordingly, the lack of functional dystrophin laying at the root of Duchenne muscular dystrophy (DMD) drives sarcolemma instability. From this point on, the cascade inevitably leading to the death of myocyte begins. In cardiomyocytes, intracellular calcium overload and related mitochondrial-mediated cell death mainly contribute to myocardial dysfunction and dilation while other protein dysregulation and/or mislocalization may affect electrical conduction system and favor arrhythmogenesis. Although clinically DMD manifests as progressive muscle weakness and skeletal muscle symptoms define characteristic of DMD, it is the heart problem the biggest challenge that most often develop in the form of dilated cardiomyopathy (DCM). Current standards of treatment and recent progress in respiratory care, introduced in most settings in the 1990s, have improved quality of life and median life expectancy to 4th decade of patient's age. At the same time, cardiac causes of death related to DMD increases. Despite preventive and palliative cardiac treatments available, the prognoses remain poor. Direct therapeutic targeting of dystrophin deficiency is critical, however, hindered by the large size of the dystrophin cDNA and/or stochastic, often extensive genetic changes in DMD gene. The correlation between cardiac involvement and mutations affecting specific dystrophin isoforms, may provide a mutation-specific cardiac management and novel therapeutic approaches for patients with CM. Nonetheless, the successful cardiac treatment poses a big challenge and may require combined therapy to combat dystrophin deficiency and its after-effects (critical in DMD pathogenesis). This review locates the multifaceted heart problem in the course of DMD, balancing the insights into basic science, translational efforts and clinical manifestation of dystrophic heart disease.
    MeSH term(s) Animals ; Arrhythmias, Cardiac/etiology ; Arrhythmias, Cardiac/metabolism ; Arrhythmias, Cardiac/pathology ; Cardiomyopathies/etiology ; Cardiomyopathies/metabolism ; Cardiomyopathies/pathology ; Dystrophin/metabolism ; Humans ; Muscular Dystrophy, Duchenne/complications
    Chemical Substances Dystrophin
    Language English
    Publishing date 2021-06-06
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 1358415-7
    ISSN 1420-9071 ; 1420-682X
    ISSN (online) 1420-9071
    ISSN 1420-682X
    DOI 10.1007/s00018-021-03862-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: CD4

    Polak, Katarzyna / Marchal, Patricia / Taroni, Chiara / Ebel, Claudine / Kirstetter, Peggy / Kastner, Philippe / Chan, Susan

    Biochemical and biophysical research communications

    2023  Volume 674, Page(s) 83–89

    Abstract: The transcriptional regulators that drive regulatory T (Treg) cell development and function remain partially understood. Helios (Ikzf2) and Eos (Ikzf4) are closely-related members of the Ikaros family of transcription factors. They are highly expressed ... ...

    Abstract The transcriptional regulators that drive regulatory T (Treg) cell development and function remain partially understood. Helios (Ikzf2) and Eos (Ikzf4) are closely-related members of the Ikaros family of transcription factors. They are highly expressed in CD4
    MeSH term(s) Animals ; Mice ; Autoimmune Diseases/genetics ; Disease Susceptibility/metabolism ; Forkhead Transcription Factors/metabolism ; Ikaros Transcription Factor/genetics ; Ikaros Transcription Factor/metabolism ; T-Lymphocytes, Regulatory ; Transcription Factors/metabolism
    Chemical Substances Forkhead Transcription Factors ; Ikaros Transcription Factor (148971-36-2) ; Transcription Factors ; IKZF2 protein, human ; EOS serine protease, human (EC 3.4.21.-)
    Language English
    Publishing date 2023-06-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 205723-2
    ISSN 1090-2104 ; 0006-291X ; 0006-291X
    ISSN (online) 1090-2104 ; 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2023.06.087
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Compromised diabetic heart function is not affected by miR-378a upregulation upon hyperglycemia.

    Florczyk-Soluch, Urszula / Polak, Katarzyna / Sabo, Reece / Martyniak, Alicja / Stępniewski, Jacek / Dulak, Józef

    Pharmacological reports : PR

    2023  Volume 75, Issue 6, Page(s) 1556–1570

    Abstract: Background: Cardiac-abundant microRNA-378a (miR-378a) is associated with postnatal repression of insulin-like growth factor 1 receptor (IGF-1R) controlling physiological hypertrophy and survival pathways. IGF-1/IGF-1R axis has been proposed as a ... ...

    Abstract Background: Cardiac-abundant microRNA-378a (miR-378a) is associated with postnatal repression of insulin-like growth factor 1 receptor (IGF-1R) controlling physiological hypertrophy and survival pathways. IGF-1/IGF-1R axis has been proposed as a therapeutic candidate against the pathophysiological progress of diabetic cardiomyopathy (DCM). We ask whether hyperglycemia-driven changes in miR-378a expression could mediate DCM progression.
    Methods: Diabetes mellitus was induced by streptozotocin (STZ) (55 mg/kg i.p. for 5 days) in male C57BL/6 wild type (miR-378a+/+) and miR-378a knockout (miR-378a-/-) mice. As a parallel human model, we harnessed human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM miR378a+/+ vs. hiPSC-CM miR378a-/-) subjected to high glucose (HG) treatment.
    Results: We reported miR-378a upregulation in cardiac diabetic milieu arising upon STZ administration to wild-type mice and in HG-treated hiPSC-CMs. Pro-hypertrophic IGF-1R/ERK1/2 pathway and hypertrophic marker expression were activated in miR-378a deficiency and upon STZ/HG treatment of miR-378a+/+ specimens in vivo and in vitro suggesting miR-378a-independent hyperglycemia-promoted hypertrophy. A synergistic upregulation of IGF-1R signaling in diabetic conditions was detected in miR-378a-/- hiPSC-CMs, but not in miR-378a-/- hearts that showed attenuation of this pathway, pointing to the involvement of compensatory mechanisms in the absence of miR-378a. Although STZ administration did not cause pro-inflammatory or pro-fibrotic effects that were detected in miR-378a-/- mice, the compromised diabetic heart function observed in vivo by high-resolution ultrasound imaging upon STZ treatment was not affected by miR-378a presence.
    Conclusions: Overall, data underline the role of miR-378a in maintaining basal cardiac structural integrity while pointing to miR-378a-independent hyperglycemia-driven cardiac hypertrophy and associated dysfunction.
    MeSH term(s) Humans ; Mice ; Male ; Animals ; Up-Regulation ; Diabetes Mellitus, Experimental/metabolism ; Mice, Inbred C57BL ; Induced Pluripotent Stem Cells/metabolism ; Hyperglycemia ; Myocytes, Cardiac ; Diabetic Cardiomyopathies/genetics ; Diabetic Cardiomyopathies/metabolism ; MicroRNAs/metabolism ; Hypertrophy/metabolism
    Chemical Substances MicroRNAs
    Language English
    Publishing date 2023-10-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2186248-5
    ISSN 2299-5684 ; 1734-1140
    ISSN (online) 2299-5684
    ISSN 1734-1140
    DOI 10.1007/s43440-023-00535-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Book ; Online: Introducing Tales of Tribute AI Competition

    Kowalski, Jakub / Miernik, Radosław / Polak, Katarzyna / Budzki, Dominik / Kowalik, Damian

    2023  

    Abstract: This paper presents a new AI challenge, the Tales of Tribute AI Competition (TOTAIC), based on a two-player deck-building card game released with the High Isle chapter of The Elder Scrolls Online. Currently, there is no other AI competition covering ... ...

    Abstract This paper presents a new AI challenge, the Tales of Tribute AI Competition (TOTAIC), based on a two-player deck-building card game released with the High Isle chapter of The Elder Scrolls Online. Currently, there is no other AI competition covering Collectible Card Games (CCG) genre, and there has never been one that targets a deck-building game. Thus, apart from usual CCG-related obstacles to overcome, like randomness, hidden information, and large branching factor, the successful approach additionally requires long-term planning and versatility. The game can be tackled with multiple approaches, including classic adversarial search, single-player planning, and Neural Networks-based algorithms. This paper introduces the competition framework, describes the rules of the game, and presents the results of a tournament between sample AI agents. The first edition of TOTAIC is hosted at the IEEE Conference on Games 2023.
    Keywords Computer Science - Artificial Intelligence
    Subject code 303
    Publishing date 2023-05-14
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Generation of human induced pluripotent stem cell lines with HMOX1 promoter polymorphism and CRISPR/Cas9-mediated deletion of exon 50 of DMD gene.

    Polak, Katarzyna / Stępniewski, Jacek / Ścieżyńska, Aneta / Podgórska, Anna / Dulak, Józef / Florczyk-Soluch, Urszula

    Stem cell research

    2022  Volume 66, Page(s) 103004

    Abstract: Duchenne muscular dystrophy (DMD), originating from the lack of functional dystrophin, clinically manifests as devastating disease of skeletal muscles with progressive cardiac involvement. HMOX1 promoter polymorphism may reflect different activity of ... ...

    Abstract Duchenne muscular dystrophy (DMD), originating from the lack of functional dystrophin, clinically manifests as devastating disease of skeletal muscles with progressive cardiac involvement. HMOX1 promoter polymorphism may reflect different activity of heme oxygenase-1 (HO-1) that may be critical for DMD progression. Here we generated human induced pluripotent stem cell (hiPSC) lines from healthy donors-derived peripheral blood mononuclear cells with different variants of HMOX1 promoter (GT repeats), and engineered by CRISPR/Cas9-mediated deletion of exon 50 of DMD gene. Such in vitro model could add to molecular understanding of DMD and verify the prognostic value of HMOX1 promoter polymorphism.
    MeSH term(s) Humans ; Induced Pluripotent Stem Cells/metabolism ; CRISPR-Cas Systems/genetics ; Heme Oxygenase-1/genetics ; Heme Oxygenase-1/metabolism ; Leukocytes, Mononuclear/metabolism ; Dystrophin/genetics ; Dystrophin/metabolism ; Muscular Dystrophy, Duchenne/metabolism ; Exons/genetics
    Chemical Substances Heme Oxygenase-1 (EC 1.14.14.18) ; Dystrophin ; HMOX1 protein, human (EC 1.14.14.18)
    Language English
    Publishing date 2022-12-13
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2393143-7
    ISSN 1876-7753 ; 1873-5061
    ISSN (online) 1876-7753
    ISSN 1873-5061
    DOI 10.1016/j.scr.2022.103004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: The Contribution of IgG Glycosylation to Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) and Complement-Dependent Cytotoxicity (CDC) in Hashimoto's Thyroiditis: An in Vitro Model of Thyroid Autoimmunity.

    Ząbczyńska, Marta / Polak, Katarzyna / Kozłowska, Kamila / Sokołowski, Grzegorz / Pocheć, Ewa

    Biomolecules

    2020  Volume 10, Issue 2

    Abstract: Antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) are involved in destruction of thyroid tissue in Hashimoto's thyroiditis (HT). ...

    Abstract Antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) are involved in destruction of thyroid tissue in Hashimoto's thyroiditis (HT).
    MeSH term(s) Antibody-Dependent Cell Cytotoxicity ; Autoantibodies/chemistry ; Autoimmunity ; Cell Line, Tumor ; Complement System Proteins/immunology ; Glycosylation ; HL-60 Cells ; Hashimoto Disease/immunology ; Humans ; Immunoglobulin G/chemistry ; Lectins/chemistry ; Leukocytes, Mononuclear/cytology ; Polysaccharides ; Sialic Acids/chemistry ; Thyroid Epithelial Cells/immunology ; Thyroid Gland/immunology ; Thyroid Gland/physiopathology
    Chemical Substances Autoantibodies ; Immunoglobulin G ; Lectins ; Polysaccharides ; Sialic Acids ; glycosylated IgG ; Complement System Proteins (9007-36-7)
    Language English
    Publishing date 2020-01-22
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom10020171
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: The relationship between structural aspects of self-concept and psychosocial adjustment in adolescents from alcoholic families.

    Polak, Katarzyna Anna / Puttler, Leon I / Ilgen, Mark Andrew

    Substance use & misuse

    2012  Volume 47, Issue 7, Page(s) 827–836

    Abstract: Sixty adolescents from alcoholic families living in two large cities in Poland were examined in 2008 and 2009. Richness, stability, and certainty of their self-concepts, as well as levels of school adjustment, anxiety, and depression, were evaluated ... ...

    Abstract Sixty adolescents from alcoholic families living in two large cities in Poland were examined in 2008 and 2009. Richness, stability, and certainty of their self-concepts, as well as levels of school adjustment, anxiety, and depression, were evaluated using a set of questionnaires. In a series of bivariate analyses, the strongest associations found were between richness of the self-concept and the social withdrawal syndrome, and between stability of the self-concept and depression. Both relationships remained significant, using multiple regression models, after controlling for possible confounding factors. Possible explanations and implications for the findings, as well as the study's limitations, are noted and discussed.
    MeSH term(s) Adolescent ; Adolescent Behavior/psychology ; Alcoholism ; Anxiety/epidemiology ; Anxiety/psychology ; Child of Impaired Parents/psychology ; Depression/epidemiology ; Depression/psychology ; Educational Status ; Family Health ; Female ; Humans ; Male ; Personality Inventory/statistics & numerical data ; Poland/epidemiology ; Psychiatric Status Rating Scales/statistics & numerical data ; Self Concept ; Social Adjustment
    Language English
    Publishing date 2012-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 1310358-1
    ISSN 1532-2491 ; 1082-6084
    ISSN (online) 1532-2491
    ISSN 1082-6084
    DOI 10.3109/10826084.2012.672536
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Biomechanical characteristics of the porcine denticulate ligament in different vertebral levels of the cervical spine-preliminary results of an experimental study.

    Polak, Katarzyna / Czyż, Marcin / Ścigała, Krzysztof / Jarmundowicz, Włodzimierz / Będziński, Romuald

    Journal of the mechanical behavior of biomedical materials

    2014  Volume 34, Page(s) 165–170

    Abstract: Background: Few studies exist on the mechanical properties of denticulate ligaments and none report the variation in these properties at different levels of the spine. The aim of this study was to perform an experimental determination of load-extension ... ...

    Abstract Background: Few studies exist on the mechanical properties of denticulate ligaments and none report the variation in these properties at different levels of the spine. The aim of this study was to perform an experimental determination of load-extension and stress-strain characteristics of the denticulate ligament and to establish if their properties change at different vertebral levels of the cervical spine.
    Method: The study was carried out on a total of 98 porcine denticulate ligament samples dissected from seven fresh porcine cervical spinal cord specimens. All of the samples were subjected to an uniaxial tensile test at a speed of 2mm/min, during which the load-extension characteristics were registered.
    Results: The analysis revealed a decrease of the failure force in the caudal orientation indicated by significant differences between the C1 (1.04±0.41N) and C7 (0.55±0.12N) vertebral levels (P=0.037). The average ultimate force that broke the denticulate ligaments was 0.88N. The mean value of Young׳s modulus was 2.06MPa with a minimum of 1.31MPa for C7 and maximum of 2.46MPa for C5.
    Conclusions: The values of the denticulate ligament failure force in samples from different cervical vertebrae levels differ significantly. The presented data should be taken into consideration during numerical modelling of the human cervical spinal cord.
    MeSH term(s) Animals ; Biomechanical Phenomena ; Cervical Vertebrae ; Humans ; Ligaments ; Materials Testing ; Mechanical Phenomena ; Stress, Mechanical ; Swine ; Tensile Strength
    Language English
    Publishing date 2014-06
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2378381-3
    ISSN 1878-0180 ; 1751-6161
    ISSN (online) 1878-0180
    ISSN 1751-6161
    DOI 10.1016/j.jmbbm.2014.02.010
    Database MEDical Literature Analysis and Retrieval System OnLINE

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