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  1. Article ; Online: SILAC-Based Proteomic Analysis of Meiosis in the Fission Yeast Schizosaccharomyces pombe.

    Anrather, Dorothea / Polakova, Silvia Bagelova / Cipak, Lubos / Gregan, Juraj

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2603, Page(s) 19–29

    Abstract: Stable isotope labeling by amino acids in cell culture (SILAC) provides a powerful tool to quantify proteins and posttranslational modifications. Here we describe how to apply SILAC for protein identification and quantification in synchronous meiotic ... ...

    Abstract Stable isotope labeling by amino acids in cell culture (SILAC) provides a powerful tool to quantify proteins and posttranslational modifications. Here we describe how to apply SILAC for protein identification and quantification in synchronous meiotic cultures induced by inactivation of the Pat1 kinase in the fission yeast Schizosaccharomyces pombe.
    MeSH term(s) Schizosaccharomyces/metabolism ; Proteomics ; Meiosis ; Proteins/metabolism ; Schizosaccharomyces pombe Proteins/genetics ; Schizosaccharomyces pombe Proteins/metabolism
    Chemical Substances Proteins ; Schizosaccharomyces pombe Proteins
    Language English
    Publishing date 2022-11-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2863-8_2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Identification of Nrl1 Domains Responsible for Interactions with RNA-Processing Factors and Regulation of Nrl1 Function by Phosphorylation.

    Mikolaskova, Barbora / Jurcik, Matus / Cipakova, Ingrid / Selicky, Tomas / Jurcik, Jan / Polakova, Silvia Bagelova / Stupenova, Erika / Dudas, Andrej / Sivakova, Barbara / Bellova, Jana / Barath, Peter / Aronica, Lucia / Gregan, Juraj / Cipak, Lubos

    International journal of molecular sciences

    2021  Volume 22, Issue 13

    Abstract: Pre-mRNA splicing is a key process in the regulation of gene expression. In the fission ... ...

    Abstract Pre-mRNA splicing is a key process in the regulation of gene expression. In the fission yeast
    MeSH term(s) Casein Kinase II/metabolism ; Phosphorylation ; Protein Binding ; Protein Interaction Domains and Motifs ; Protein Interaction Mapping ; RNA Processing, Post-Transcriptional ; RNA Splicing ; RNA-Binding Proteins/metabolism ; Schizosaccharomyces/genetics ; Schizosaccharomyces/metabolism ; Schizosaccharomyces pombe Proteins/chemistry ; Schizosaccharomyces pombe Proteins/genetics ; Schizosaccharomyces pombe Proteins/metabolism ; Schizosaccharomyces pombe Proteins/physiology ; Spliceosomes/metabolism ; Two-Hybrid System Techniques
    Chemical Substances Nrl1 protein, S pombe ; RNA-Binding Proteins ; Schizosaccharomyces pombe Proteins ; Casein Kinase II (EC 2.7.11.1)
    Language English
    Publishing date 2021-06-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms22137011
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Proteomic analysis of meiosis and characterization of novel short open reading frames in the fission yeast

    Huraiova, Barbora / Kanovits, Judit / Polakova, Silvia Bagelova / Cipak, Lubos / Benko, Zsigmond / Sevcovicova, Andrea / Anrather, Dorothea / Ammerer, Gustav / Duncan, Caia D S / Mata, Juan / Gregan, Juraj

    Cell cycle (Georgetown, Tex.)

    2020  Volume 19, Issue 14, Page(s) 1777–1785

    Abstract: Meiosis is the process by which haploid gametes are produced from diploid precursor cells. We used stable isotope labeling by amino acids in cell culture (SILAC) to characterize the meiotic proteome in the fission ... ...

    Abstract Meiosis is the process by which haploid gametes are produced from diploid precursor cells. We used stable isotope labeling by amino acids in cell culture (SILAC) to characterize the meiotic proteome in the fission yeast
    MeSH term(s) Gene Deletion ; Isotope Labeling ; Meiosis/genetics ; Open Reading Frames/genetics ; Phenotype ; Proteome/metabolism ; Proteomics ; Reproducibility of Results ; Ribosomes/metabolism ; Schizosaccharomyces/cytology ; Schizosaccharomyces/genetics ; Schizosaccharomyces pombe Proteins/genetics ; Schizosaccharomyces pombe Proteins/metabolism
    Chemical Substances Proteome ; Schizosaccharomyces pombe Proteins
    Language English
    Publishing date 2020-06-17
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2146183-1
    ISSN 1551-4005 ; 1538-4101 ; 1554-8627
    ISSN (online) 1551-4005
    ISSN 1538-4101 ; 1554-8627
    DOI 10.1080/15384101.2020.1779470
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5881: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Repression of a large number of genes requires interplay between homologous recombination and HIRA.

    Misova, Ivana / Pitelova, Alexandra / Budis, Jaroslav / Gazdarica, Juraj / Sedlackova, Tatiana / Jordakova, Anna / Benko, Zsigmond / Smondrkova, Maria / Mayerova, Nina / Pichlerova, Karoline / Strieskova, Lucia / Prevorovsky, Martin / Gregan, Juraj / Cipak, Lubos / Szemes, Tomas / Polakova, Silvia Bagelova

    Nucleic acids research

    2021  Volume 49, Issue 4, Page(s) 1914–1934

    Abstract: During homologous recombination, Dbl2 protein is required for localisation of Fbh1, an F-box helicase that efficiently dismantles Rad51-DNA filaments. RNA-seq analysis of dbl2Δ transcriptome showed that the dbl2 deletion results in upregulation of more ... ...

    Abstract During homologous recombination, Dbl2 protein is required for localisation of Fbh1, an F-box helicase that efficiently dismantles Rad51-DNA filaments. RNA-seq analysis of dbl2Δ transcriptome showed that the dbl2 deletion results in upregulation of more than 500 loci in Schizosaccharomyces pombe. Compared with the loci with no change in expression, the misregulated loci in dbl2Δ are closer to long terminal and long tandem repeats. Furthermore, the misregulated loci overlap with antisense transcripts, retrotransposons, meiotic genes and genes located in subtelomeric regions. A comparison of the expression profiles revealed that Dbl2 represses the same type of genes as the HIRA histone chaperone complex. Although dbl2 deletion does not alleviate centromeric or telomeric silencing, it suppresses the silencing defect at the outer centromere caused by deletion of hip1 and slm9 genes encoding subunits of the HIRA complex. Moreover, our analyses revealed that cells lacking dbl2 show a slight increase of nucleosomes at transcription start sites and increased levels of methylated histone H3 (H3K9me2) at centromeres, subtelomeres, rDNA regions and long terminal repeats. Finally, we show that other proteins involved in homologous recombination, such as Fbh1, Rad51, Mus81 and Rad54, participate in the same gene repression pathway.
    MeSH term(s) Cell Cycle Proteins/antagonists & inhibitors ; Cell Cycle Proteins/metabolism ; Centromere ; Gene Expression Regulation, Fungal ; Gene Silencing ; Histone Code ; Homologous Recombination ; Nucleosomes/metabolism ; Repressor Proteins/physiology ; Schizosaccharomyces/genetics ; Schizosaccharomyces pombe Proteins/antagonists & inhibitors ; Schizosaccharomyces pombe Proteins/metabolism ; Schizosaccharomyces pombe Proteins/physiology ; Transcription Factors/antagonists & inhibitors ; Transcription Factors/metabolism
    Chemical Substances Cell Cycle Proteins ; Dbl2 protein, S pombe ; Nucleosomes ; Repressor Proteins ; Schizosaccharomyces pombe Proteins ; Slm9 protein, S pombe ; Transcription Factors ; hip1 protein, S pombe
    Language English
    Publishing date 2021-03-09
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 186809-3
    ISSN 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
    ISSN (online) 1362-4962 ; 1362-4954
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkab027
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1067: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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