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Article ; Online: Growth and mortality of aerobic anoxygenic phototrophs in the North Pacific Subtropical Gyre.

Koblížek, Michal / Ferrera, Isabel / Kolářová, Eva / Duhamel, Solange / Popendorf, Kimberly J / Gasol, Josep M / Van Mooy, Benjamin A S

Applied and environmental microbiology

2024 Volume 90, Issue 4, Page(s) e0003224

Abstract: Aerobic anoxygenic phototrophic (AAP) bacteria harvest light energy using bacteriochlorophyll-containing reaction centers to supplement their mostly heterotrophic metabolism. While their abundance and growth have been intensively studied in coastal ... ...

Abstract	Aerobic anoxygenic phototrophic (AAP) bacteria harvest light energy using bacteriochlorophyll-containing reaction centers to supplement their mostly heterotrophic metabolism. While their abundance and growth have been intensively studied in coastal environments, much less is known about their activity in oligotrophic open ocean regions. Therefore, we combined
MeSH term(s)	Bacteriochlorophylls/metabolism ; Phototrophic Processes ; Bacteria, Aerobic ; Water/metabolism ; Nitrogen/metabolism ; Seawater/microbiology
Chemical Substances	Bacteriochlorophylls ; Water (059QF0KO0R) ; Nitrogen (N762921K75)
Language	English
Publishing date	2024-03-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	223011-2
ISSN	1098-5336 ; 0099-2240
ISSN (online)	1098-5336
ISSN	0099-2240
DOI	10.1128/aem.00032-24
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Article ; Online: Variable phosphorus uptake rates and allocation across microbial groups in the oligotrophic Gulf of Mexico.

Popendorf, Kimberly J / Duhamel, Solange

Environmental microbiology

2015 Volume 17, Issue 10, Page(s) 3992–4006

Abstract: Microbial uptake of dissolved phosphorus (P) is an important lever in controlling both microbial production and the fate and cycling of marine P. We investigated the relative role of heterotrophic bacteria and phytoplankton in P cycling by measuring the ... ...

Abstract	Microbial uptake of dissolved phosphorus (P) is an important lever in controlling both microbial production and the fate and cycling of marine P. We investigated the relative role of heterotrophic bacteria and phytoplankton in P cycling by measuring the P uptake rates of individual microbial groups (heterotrophic bacteria and the phytoplankton groups Synechococcus, Prochlorococcus and picoeukaryotic phytoplankton) in the P-depleted Gulf of Mexico. Phosphorus uptake rates were measured using incubations with radiolabelled phosphate and adenosine triphosphate coupled with cell sorting flow cytometry. We found that heterotrophic bacteria were the dominant consumers of P on both a biomass basis and a population basis. Biovolume normalized heterotrophic bacteria P uptake rate per cell (amol P μm(-3) h(-1)) was roughly an order of magnitude greater than phytoplankton uptake rates, and heterotrophic bacteria were responsible for generally greater than 50% of total picoplankton P uptake. We hypothesized that this variation in uptake rates reflects variation in cellular P allocation strategies, and found that, indeed, the fraction of cellular P uptake utilized for phospholipid production was significantly higher in heterotrophic bacteria compared with cyanobacterial phytoplankton. These findings indicate that heterotrophic bacteria have a uniquely P-oriented physiology and play a dominant role in cycling dissolved P.
MeSH term(s)	Adenosine Triphosphate/metabolism ; Biological Transport ; Biomass ; Flow Cytometry ; Gulf of Mexico ; Heterotrophic Processes ; Mexico ; Phosphates/metabolism ; Phosphorus/metabolism ; Phytoplankton/metabolism ; Prochlorococcus/metabolism ; Seawater/microbiology ; Synechococcus/metabolism
Chemical Substances	Phosphates ; Phosphorus (27YLU75U4W) ; Adenosine Triphosphate (8L70Q75FXE)
Language	English
Publishing date	2015-10
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2020213-1
ISSN	1462-2920 ; 1462-2912
ISSN (online)	1462-2920
ISSN	1462-2912
DOI	10.1111/1462-2920.12932
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Article: Variable phosphorus uptake rates and allocation across microbial groups in the oligotrophic Gulf of Mexico

Popendorf, Kimberly J / Duhamel, Solange

Environmental microbiology. 2015 Oct., v. 17, no. 10

2015

Abstract	Microbial uptake of dissolved phosphorus (P) is an important lever in controlling both microbial production and the fate and cycling of marine P. We investigated the relative role of heterotrophic bacteria and phytoplankton in P cycling by measuring the P uptake rates of individual microbial groups (heterotrophic bacteria and the phytoplankton groups Synechococcus, Prochlorococcus and picoeukaryotic phytoplankton) in the P‐depleted Gulf of Mexico. Phosphorus uptake rates were measured using incubations with radiolabelled phosphate and adenosine triphosphate coupled with cell sorting flow cytometry. We found that heterotrophic bacteria were the dominant consumers of P on both a biomass basis and a population basis. Biovolume normalized heterotrophic bacteria P uptake rate per cell (amol P μm⁻³ h⁻¹) was roughly an order of magnitude greater than phytoplankton uptake rates, and heterotrophic bacteria were responsible for generally greater than 50% of total picoplankton P uptake. We hypothesized that this variation in uptake rates reflects variation in cellular P allocation strategies, and found that, indeed, the fraction of cellular P uptake utilized for phospholipid production was significantly higher in heterotrophic bacteria compared with cyanobacterial phytoplankton. These findings indicate that heterotrophic bacteria have a uniquely P‐oriented physiology and play a dominant role in cycling dissolved P.
Keywords	Prochlorococcus ; Synechococcus ; adenosine triphosphate ; bacteria ; biomass ; flow cytometry ; phospholipids ; phosphorus ; physiology ; phytoplankton ; radiolabeling ; Gulf of Mexico
Language	English
Dates of publication	2015-10
Size	p. 3992-4006.
Publishing place	Blackwell Science
Document type	Article
ZDB-ID	2020213-1
ISSN	1462-2920 ; 1462-2912
ISSN (online)	1462-2920
ISSN	1462-2912
DOI	10.1111/1462-2920.12932
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Article: Harmful cyanobacterial aerosolization dynamics in the airshed of a eutrophic estuary

Plaas, Haley E. / Paerl, Ryan W. / Baumann, Karsten / Karl, Colleen / Popendorf, Kimberly J. / Barnard, Malcolm A. / Chang, Naomi Y. / Curtis, Nathaniel P. / Huang, Hwa / Mathieson, Olivia L. / Sanchez, Joel / Maizel, Daniela J. / Bartenfelder, Amy N. / Braddy, Jeremy S. / Hall, Nathan S. / Rossignol, Karen L. / Sloup, Randolph / Paerl, Hans W.

Science of the total environment. 2022 Dec. 15, v. 852

2022

Abstract: In addition to obvious negative effects on water quality in eutrophic aquatic ecosystems, recent work suggests that cyanobacterial harmful algal blooms (CHABs) also impact air quality via emissions carrying cyanobacterial cells and cyanotoxins. However, ... ...

Abstract	In addition to obvious negative effects on water quality in eutrophic aquatic ecosystems, recent work suggests that cyanobacterial harmful algal blooms (CHABs) also impact air quality via emissions carrying cyanobacterial cells and cyanotoxins. However, the environmental controls on CHAB-derived aerosol and its potential public health impacts remain largely unknown. Accordingly, the aims of this study were to 1) investigate the occurrence of microcystins (MC) and putatively toxic cyanobacterial communities in particulate matter ≤ 2.5 μm in diameter (PM₂.₅), 2) elucidate environmental conditions promoting their aerosolization, and 3) identify associations between CHABs and PM₂.₅ concentrations in the airshed of the Chowan River-Albemarle Sound, an oligohaline, eutrophic estuary in eastern North Carolina, USA. In summer 2020, during peak CHAB season, continuous PM₂.₅ samples and interval water samples were collected at two distinctive sites for targeted analyses of cyanobacterial community composition and MC concentration. Supporting air and water quality measurements were made in parallel to contextualize findings and permit statistical analyses of environmental factors driving changes in CHAB-derived aerosol. MC concentrations were low throughout the study, but a CHAB dominated by Dolichospermum occurred from late June to early August. Several aquatic CHAB genera recovered from Chowan River surface water were identified in PM₂.₅ during multiple time points, including Anabaena, Aphanizomenon, Dolichospermum, Microcystis, and Pseudanabaena. Cyanobacterial enrichment in PM₂.₅ was indistinctive between subspecies, but at one site during the early bloom, we observed the simultaneous enrichment of several cyanobacterial genera in PM₂.₅. In association with the CHAB, the median PM₂.₅ mass concentration increased to 8.97 μg m⁻³ (IQR = 5.15), significantly above the non-bloom background of 5.35 μg m⁻³ (IQR = 3.70) (W = 1835, p < 0.001). Results underscore the need for highly resolved temporal measurements to conclusively investigate the role that CHABs play in regional air quality and respiratory health risk.
Keywords	Anabaena ; Aphanizomenon ; Dolichospermum ; Microcystis ; Pseudanabaena ; aerosols ; air ; air quality ; airshed ; community structure ; environment ; estuaries ; eutrophication ; microcystins ; particulates ; poisonous algae ; public health ; risk ; rivers ; summer ; surface water ; toxicity ; water quality ; North Carolina
Language	English
Dates of publication	2022-1215
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2022.158383
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Article ; Online: Exposure to Aerosolized Algal Toxins in South Florida Increases Short- and Long-Term Health Risk in

Hu, Jiaming / Liu, Jiaqi / Zhu, Yi / Diaz-Perez, Zoraida / Sheridan, Michael / Royer, Haley / Leibensperger, Raymond / Maizel, Daniela / Brand, Larry / Popendorf, Kimberly J / Gaston, Cassandra J / Zhai, R Grace

Toxins

2020 Volume 12, Issue 12

Abstract: Harmful algal blooms (HABs) are a rising health and environmental concern in the United States, particularly in South Florida. Skin contact and the ingestion of contaminated water or fish and other seafood have been proven to have severe toxicity to ... ...

Abstract	Harmful algal blooms (HABs) are a rising health and environmental concern in the United States, particularly in South Florida. Skin contact and the ingestion of contaminated water or fish and other seafood have been proven to have severe toxicity to humans in some cases. However, the impact of aerosolized HAB toxins is poorly understood. In particular, knowledge regarding either the immediate or long-term effects of exposure to aerosolized cyanotoxins produced by freshwater blue-green algae does not exist. The aim of this study was to probe the toxicity of aerosolized cyanobacterial blooms using
MeSH term(s)	Aerosols ; Aging/drug effects ; Aging/pathology ; Aging/physiology ; Animals ; Drosophila ; Female ; Florida ; Harmful Algal Bloom ; Longevity/drug effects ; Longevity/physiology ; Male ; Microcystins/analysis ; Microcystins/toxicity ; Models, Animal ; Risk Assessment/methods ; Risk Factors ; Time Factors ; Water Pollution/adverse effects
Chemical Substances	Aerosols ; Microcystins ; microcystin (77238-39-2)
Language	English
Publishing date	2020-12-11
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2518395-3
ISSN	2072-6651 ; 2072-6651
ISSN (online)	2072-6651
ISSN	2072-6651
DOI	10.3390/toxins12120787
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Article ; Online: Harmful cyanobacterial aerosolization dynamics in the airshed of a eutrophic estuary.

Plaas, Haley E / Paerl, Ryan W / Baumann, Karsten / Karl, Colleen / Popendorf, Kimberly J / Barnard, Malcolm A / Chang, Naomi Y / Curtis, Nathaniel P / Huang, Hwa / Mathieson, Olivia L / Sanchez, Joel / Maizel, Daniela J / Bartenfelder, Amy N / Braddy, Jeremy S / Hall, Nathan S / Rossignol, Karen L / Sloup, Randolph / Paerl, Hans W

The Science of the total environment

2022 Volume 852, Page(s) 158383

Abstract	In addition to obvious negative effects on water quality in eutrophic aquatic ecosystems, recent work suggests that cyanobacterial harmful algal blooms (CHABs) also impact air quality via emissions carrying cyanobacterial cells and cyanotoxins. However, the environmental controls on CHAB-derived aerosol and its potential public health impacts remain largely unknown. Accordingly, the aims of this study were to 1) investigate the occurrence of microcystins (MC) and putatively toxic cyanobacterial communities in particulate matter ≤ 2.5 μm in diameter (PM
MeSH term(s)	Microcystins/analysis ; Estuaries ; Lakes/microbiology ; Ecosystem ; Cyanobacteria ; Harmful Algal Bloom ; Particulate Matter/analysis
Chemical Substances	Microcystins ; Particulate Matter
Language	English
Publishing date	2022-09-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2022.158383
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Article ; Online: Molecular ion-independent quantification of polar glycerolipid classes in marine plankton using triple quadrupole MS.

Popendorf, Kimberly J / Fredricks, Helen F / Van Mooy, Benjamin A S

Lipids

2012 Volume 48, Issue 2, Page(s) 185–195

Abstract: Polar glycerolipids are a diverse family of lipid molecules that form the bulk of bacterial and eukaryotic microbial membranes. The earth and ocean sciences has a long history of using fatty acids as biomarkers for microbes, but have only recently begun ... ...

Abstract	Polar glycerolipids are a diverse family of lipid molecules that form the bulk of bacterial and eukaryotic microbial membranes. The earth and ocean sciences has a long history of using fatty acids as biomarkers for microbes, but have only recently begun to examine the intact polar lipids from which they are derived. Current analytical approaches rely on laboriously quantifying the molecular ions of each of these species independently. Thus, we saw a need for a method for quantifying polar glycerolipid classes that was: (i) selective for individual classes, (ii) inclusive of all species within a class, (iii) independent of foreknowledge of the molecular ions of the polar glycerolipid, and (iv) amenable to automated, high-throughput data analysis methods. Our new HPLC-electrospray-ionization triple-quadrupole MS (HPLC-ESI-TQMS) method can be applied to quantify the nine major classes of polar glycerolipid in planktonic communities: the phospholipids phosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine; the glycolipids monoglycosyldiacylglycerol, diglycosyldiacylglycerol and sulfoquinovosyldiacylglycerol; and the betaine lipids diacylglyceryl trimethyl homoserine, diacylglyceryl hydroxymethyl trimethyl-β-alanine, and diacylglyceryl carboxyhydroxymethylcholine. The analyses rely on neutral loss and parent ion scan events that yield one chromatogram for each class of polar glycerolipid, simplifying downstream analysis and increasing sample throughput. The efficacy of the method was demonstrated by analyzing plankton community samples from a variety of marine environments.
MeSH term(s)	Chromatography, High Pressure Liquid ; Glycolipids/analysis ; Plankton/chemistry ; Spectrometry, Mass, Electrospray Ionization/methods
Chemical Substances	Glycolipids
Language	English
Publishing date	2012-12-27
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	241539-2
ISSN	1558-9307 ; 0024-4201
ISSN (online)	1558-9307
ISSN	0024-4201
DOI	10.1007/s11745-012-3748-0
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Article ; Online: African biomass burning is a substantial source of phosphorus deposition to the Amazon, Tropical Atlantic Ocean, and Southern Ocean.

Barkley, Anne E / Prospero, Joseph M / Mahowald, Natalie / Hamilton, Douglas S / Popendorf, Kimberly J / Oehlert, Amanda M / Pourmand, Ali / Gatineau, Alexandre / Panechou-Pulcherie, Kathy / Blackwelder, Patricia / Gaston, Cassandra J

Proceedings of the National Academy of Sciences of the United States of America

2019 Volume 116, Issue 33, Page(s) 16216–16221

Abstract: The deposition of phosphorus (P) from African dust is believed to play an important role in bolstering primary productivity in the Amazon Basin and Tropical Atlantic Ocean (TAO), leading to sequestration of carbon dioxide. However, there are few ... ...

Abstract	The deposition of phosphorus (P) from African dust is believed to play an important role in bolstering primary productivity in the Amazon Basin and Tropical Atlantic Ocean (TAO), leading to sequestration of carbon dioxide. However, there are few measurements of African dust in South America that can robustly test this hypothesis and even fewer measurements of soluble P, which is readily available for stimulating primary production in the ocean. To test this hypothesis, we measured total and soluble P in long-range transported aerosols collected in Cayenne, French Guiana, a TAO coastal site located at the northeastern edge of the Amazon. Our measurements confirm that in boreal spring when African dust transport is greatest, dust supplies the majority of P, of which 5% is soluble. In boreal fall, when dust transport is at an annual minimum, we measured unexpectedly high concentrations of soluble P, which we show is associated with the transport of biomass burning (BB) from southern Africa. Integrating our results into a chemical transport model, we show that African BB supplies up to half of the P deposited annually to the Amazon from transported African aerosol. This observational study links P-rich BB aerosols from Africa to enhanced P deposition in the Amazon. Contrary to current thought, we also show that African BB is a more important source of soluble P than dust to the TAO and oceans in the Southern Hemisphere and may be more important for marine productivity, particularly in boreal summer and fall.
MeSH term(s)	Aerosols/chemistry ; Africa, Southern ; Air Pollutants/analysis ; Atlantic Ocean ; Atmosphere ; Biomass ; Carbon Dioxide/adverse effects ; Carbon Dioxide/metabolism ; Dust/analysis ; Environmental Monitoring ; French Guiana ; Oceans and Seas ; Phosphorus/metabolism ; Seasons ; South America
Chemical Substances	Aerosols ; Air Pollutants ; Dust ; Carbon Dioxide (142M471B3J) ; Phosphorus (27YLU75U4W)
Language	English
Publishing date	2019-07-29
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	209104-5
ISSN	1091-6490 ; 0027-8424
ISSN (online)	1091-6490
ISSN	0027-8424
DOI	10.1073/pnas.1906091116
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Zs.A 1148: Show issues

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Article: Molecular Ion-Independent Quantification of Polar Glycerolipid Classes in Marine Plankton Using Triple Quadrupole MS

Popendorf, Kimberly J / Fredricks, Helen F / Van Mooy, Benjamin A. S

Lipids. 2013 Feb., v. 48, no. 2

2013

Abstract	Polar glycerolipids are a diverse family of lipid molecules that form the bulk of bacterial and eukaryotic microbial membranes. The earth and ocean sciences has a long history of using fatty acids as biomarkers for microbes, but have only recently begun to examine the intact polar lipids from which they are derived. Current analytical approaches rely on laboriously quantifying the molecular ions of each of these species independently. Thus, we saw a need for a method for quantifying polar glycerolipid classes that was: (i) selective for individual classes, (ii) inclusive of all species within a class, (iii) independent of foreknowledge of the molecular ions of the polar glycerolipid, and (iv) amenable to automated, high-throughput data analysis methods. Our new HPLC-electrospray-ionization triple-quadrupole MS (HPLC-ESI-TQMS) method can be applied to quantify the nine major classes of polar glycerolipid in planktonic communities: the phospholipids phosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine; the glycolipids monoglycosyldiacylglycerol, diglycosyldiacylglycerol and sulfoquinovosyldiacylglycerol; and the betaine lipids diacylglyceryl trimethyl homoserine, diacylglyceryl hydroxymethyl trimethyl-β-alanine, and diacylglyceryl carboxyhydroxymethylcholine. The analyses rely on neutral loss and parent ion scan events that yield one chromatogram for each class of polar glycerolipid, simplifying downstream analysis and increasing sample throughput. The efficacy of the method was demonstrated by analyzing plankton community samples from a variety of marine environments.
Keywords	betaine ; biomarkers ; cell membranes ; electrospray ionization mass spectrometry ; eukaryotic cells ; fatty acids ; glycolipids ; high performance liquid chromatography ; ions ; marine ecosystems ; microorganisms ; oceans ; phosphatidylcholines ; phosphatidylethanolamines ; plankton ; prokaryotic cells
Language	English
Dates of publication	2013-02
Size	p. 185-195.
Publishing place	Springer-Verlag
Document type	Article
ZDB-ID	241539-2
ISSN	1558-9307 ; 0024-4201
ISSN (online)	1558-9307
ISSN	0024-4201
DOI	10.1007/s11745-012-3748-0
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Article: Microbial sources of intact polar diacylglycerolipids in the Western North Atlantic Ocean

Popendorf, Kimberly J / Lomas, Michael W / Van Mooy, Benjamin A.S

Organic geochemistry. 2011 Aug., v. 42, no. 7

2011

Abstract: Intact polar membrane lipids are essential components of microbial membranes and recent work has uncovered a diversity of them occurring in the ocean. While it has long been understood that lipid composition varies across microbial groups, the microbial ... ...

Abstract	Intact polar membrane lipids are essential components of microbial membranes and recent work has uncovered a diversity of them occurring in the ocean. While it has long been understood that lipid composition varies across microbial groups, the microbial origins of the intact polar lipids in the surface ocean remain to be fully explained. This study focused on identifying the microbial sources of intact polar diacylglycerolipids (IP-DAGs) in the surface waters of the western North Atlantic Ocean. We used three approaches to define these microbial sources: (i) ¹³C tracing to identify photoautotrophic and heterotrophic production of the major classes of IP-DAGs, (ii) cell sorting flow cytometry of Prochlorococcus, Synechococcus and heterotrophic bacteria to determine IP-DAG composition and (iii) regrowth incubations targeting IP-DAG production by heterotrophic bacteria. Stable isotope tracing indicated that sulfoquinovosyldiacylglycerol (SQDG) and diacylglyceryl-trimethyl-homoserine (DGTS) were produced predominantly by photoautotrophs, while phosphatidylglycerol (PG) production was dominated by heterotrophic bacteria. Of the cells sorted with flow cytometry, Prochlorococcus and Synechococcus were found to have abundant glycolipids, while heterotrophic bacteria were dominated by phospholipids. The regrowth incubations showed that the growth of heterotrophic bacteria correlated with an increase in the concentration of PG, phosphatidylethanolamine (PE) and monoglycosyldiacylglycerol (MGDG). The finding of MGDG in heterotrophic bacteria differs from previous work, which had asserted that the membranes of heterotrophic bacteria in this environment were composed entirely of phospholipids. Overall, our findings indicate that phytoplankton are the primary source of SQDG and DGTS, while heterotrophic bacteria are the dominant source of PG, making these three compounds promising biomarkers for the study of microbes in the surface ocean.
Keywords	Prochlorococcus ; Synechococcus ; autotrophs ; bacteria ; biomarkers ; flow cytometry ; glycolipids ; isotope labeling ; lipid composition ; phosphatidylethanolamines ; phytoplankton ; regrowth ; sorting ; stable isotopes ; surface water ; Atlantic Ocean
Language	English
Dates of publication	2011-08
Size	p. 803-811.
Publishing place	Elsevier Ltd
Document type	Article
ISSN	0146-6380
DOI	10.1016/j.orggeochem.2011.05.003
Database	NAL-Catalogue (AGRICOLA)

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