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  1. Article ; Online: Probing the conformational dynamics of an Ago-RNA complex in water/methanol solution.

    Porcelli, Francesco / Casavola, Anna Rita / Grottesi, Alessandro / Schiumarini, Donatella / Avaldi, Lorenzo

    Physical chemistry chemical physics : PCCP

    2024  Volume 26, Issue 3, Page(s) 2497–2508

    Abstract: Argonaute (Ago) proteins mediate target recognition guiding miRNA to bind complementary mRNA primarily in the seed region. However, additional pairing can occur beyond the seed, forming a supplementary duplex that can contribute to the guide-target ... ...

    Abstract Argonaute (Ago) proteins mediate target recognition guiding miRNA to bind complementary mRNA primarily in the seed region. However, additional pairing can occur beyond the seed, forming a supplementary duplex that can contribute to the guide-target affinity. In order to shed light on the connection, between protein-RNA interactions and miRNA-mRNA seed and supplementary duplex mobility, we carried out molecular dynamics simulations at the microsecond time-scale using a different approach compared to the ones normally used. Until now, theoretical investigations with classical MD on Ago-RNA complexes have been focused primarily on pure water solvent, which mimics the natural environment of biological molecules. Here, we explored the conformational space of a human Ago2 (hAgo2) bound to the seed + supplementary miRNA-mRNA duplex, using the solvent environment as a molecular probe. MD simulations have been performed in a mixture of water/MeOH at a molar ratio of 70 : 30 as well as in pure water for comparison. Our findings revealed that the mixed solvent promotes protein RNA association, principally enhancing salt-linkages between basic amino acid side-chains and acidic phosphates of the sugar-phosphate backbone. The primary effect registered was the restriction of supplementary duplex flexibility and the stabilization of the miRNA 3' terminus. Interestingly, we observed that the influence of the solvent appears to have almost no impact on the conformation of the seed duplex.
    MeSH term(s) Humans ; Methanol ; Protein Binding ; MicroRNAs/chemistry ; RNA, Messenger/chemistry ; Solvents
    Chemical Substances Methanol (Y4S76JWI15) ; MicroRNAs ; RNA, Messenger ; Solvents
    Language English
    Publishing date 2024-01-17
    Publishing country England
    Document type Journal Article
    ZDB-ID 1476244-4
    ISSN 1463-9084 ; 1463-9076
    ISSN (online) 1463-9084
    ISSN 1463-9076
    DOI 10.1039/d3cp05530b
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Revenue vs expenditure based fiscal consolidation: the pass-through from federal cuts to local taxes.

    Marattin, Luigi / Nannicini, Tommaso / Porcelli, Francesco

    International tax and public finance

    2021  Volume 29, Issue 4, Page(s) 834–872

    Abstract: A growing literature emphasizes that the output effect of fiscal consolidation hinges on its composition, as the choice of increasing revenues vs cutting expenditure is not neutral. Existing studies, however, underscore the role of local governments in a ...

    Abstract A growing literature emphasizes that the output effect of fiscal consolidation hinges on its composition, as the choice of increasing revenues vs cutting expenditure is not neutral. Existing studies, however, underscore the role of local governments in a federal setting. Indeed, transfer cuts at the central level might translate into higher local taxes, changing the effective composition of the fiscal adjustment. We evaluate this transmission mechanism in Italy, where municipalities below the threshold of 5,000 inhabitants were exempted from (large) transfer cuts in 2012. This allows us to implement a difference-in-discontinuities design in order to estimate the causal impact of transfer cuts on the composition of fiscal adjustment, also because tight fiscal rules impose a balanced budget on Italian municipalities. We find a pass-through mechanism by which local governments react to the contraction of intergovernmental grants by mainly increasing taxes rather than reducing spending. From a political economy perspective, this revenue based fiscal consolidation is driven by local governments with low electoral competition and low party fragmentation.
    Language English
    Publishing date 2021-09-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2016247-9
    ISSN 1573-6970 ; 0927-5940
    ISSN (online) 1573-6970
    ISSN 0927-5940
    DOI 10.1007/s10797-021-09682-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Correction: Lozano-Soria et al. Volatile Organic Compounds from Entomopathogenic and Nematophagous Fungi, Repel Banana Black Weevil (

    Lozano-Soria, Ana / Picciotti, Ugo / Lopez-Moya, Federico / Lopez-Cepero, Javier / Porcelli, Francesco / Lopez-Llorca, Luis Vicente

    Insects

    2024  Volume 15, Issue 3

    Abstract: In the original publication [ ... ]. ...

    Abstract In the original publication [...].
    Language English
    Publishing date 2024-03-05
    Publishing country Switzerland
    Document type Published Erratum
    ZDB-ID 2662247-6
    ISSN 2075-4450
    ISSN 2075-4450
    DOI 10.3390/insects15030175
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Brindley’s Glands Volatilome of the Predator Zelus renardii Interacting with Xylella Vectors

    Picciotti, Ugo / Valverde-Urrea, Miguel / Garganese, Francesca / Lopez-Moya, Federico / Foubelo, Francisco / Porcelli, Francesco / Lopez-Llorca, Luis Vicente

    Insects. 2023 June 03, v. 14, no. 6

    2023  

    Abstract: Alien species must adapt to new biogeographical regions to acclimatise and survive. We consider a species to have become invasive if it establishes negative interactions after acclimatisation. Xylella fastidiosa Wells, Raju et al., 1986 (XF) represents ... ...

    Abstract Alien species must adapt to new biogeographical regions to acclimatise and survive. We consider a species to have become invasive if it establishes negative interactions after acclimatisation. Xylella fastidiosa Wells, Raju et al., 1986 (XF) represents Italy’s and Europe’s most recent biological invasion. In Apulia (southern Italy), the XF-encountered Philaenus spumarius L. 1758 (Spittlebugs, Hemiptera: Auchenorrhyncha) can acquire and transmit the bacterium to Olea europaea L., 1753. The management of XF invasion involves various transmission control means, including inundative biological control using Zelus renardii (ZR) Kolenati, 1856 (Hemiptera: Reduviidae). ZR is an alien stenophagous predator of Xylella vectors, recently entered from the Nearctic and acclimated in Europe. Zelus spp. can secrete semiochemicals during interactions with conspecifics and prey, including volatile organic compounds (VOCs) that elicit conspecific defence behavioural responses. Our study describes ZR Brindley’s glands, present in males and females of ZR, which can produce semiochemicals, eliciting conspecific behavioural responses. We scrutinised ZR secretion alone or interacting with P. spumarius. The ZR volatilome includes 2-methyl-propanoic acid, 2-methyl-butanoic acid, and 3-methyl-1-butanol, which are consistent for Z. renardii alone. Olfactometric tests show that these three VOCs, individually tested, generate an avoidance (alarm) response in Z. renardii. 3-Methyl-1-butanol elicited the highest significant repellence, followed by 2-methyl-butanoic and 2-methyl-propanoic acids. The concentrations of the VOCs of ZR decrease during the interaction with P. spumarius. We discuss the potential effects of VOC secretions on the interaction of Z. renardii with P. spumarius.
    Keywords Cercopidae ; Nearctic region ; Olea europaea ; Philaenus spumarius ; Xylella fastidiosa ; Zelus renardii ; acclimation ; bacteria ; biological control ; conspecificity ; ecological invasion ; geographical distribution ; introduced species ; olfactometry ; secretion ; semiochemicals ; volatile organic compounds ; Italy
    Language English
    Dates of publication 2023-0603
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2662247-6
    ISSN 2075-4450
    ISSN 2075-4450
    DOI 10.3390/insects14060520
    Database NAL-Catalogue (AGRICOLA)

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  5. Article: Zelus renardii Roaming in Southern Italy

    Lahbib, Nada / Picciotti, Ugo / Sefa, Valdete / Boukhris-Bouhachem, Sonia / Porcelli, Francesco / Garganese, Francesca

    Insects. 2022 Jan. 31, v. 13, no. 2

    2022  

    Abstract: This study collects data from the literature and updates our Zelus renardii Kolenati, 1856 (Leafhopper Assassin Bug, LAB) prey knowledge. The literature consists of ca. 170 entries encompassing the years 1856 to 2021. This reduviid originated in the ... ...

    Abstract This study collects data from the literature and updates our Zelus renardii Kolenati, 1856 (Leafhopper Assassin Bug, LAB) prey knowledge. The literature consists of ca. 170 entries encompassing the years 1856 to 2021. This reduviid originated in the Nearctic region, but has entered and acclimatised in many Mediterranean countries. Our quantitative predation experiments—in the laboratory on caged plants plus field or environmental observations—confirm that LAB prefers a selected array of prey. Laboratory predation tests on living targets (Hemiptera, Coleoptera, Diptera, and Hymenoptera) agree with the literature. Zelus renardii prefers comparatively large, highly mobile, and readily available prey. LAB preferences on available hemipterans targets suggest that Zelus renardii is a good inundative biocontrol agent for Xylella fastidiosapauca ST53 infections. LAB also prey on other important olive pests, such as Bactrocera oleae. Therefore, Zelus renardii is a major integrated pest management (IPM) component to limit Xylella fastidiosa pandemics and other pest invasions.
    Keywords Bactrocera oleae ; Coleoptera ; Hymenoptera ; Nearctic region ; Xylella fastidiosa ; Zelus renardii ; biological control agents ; integrated pest management ; olives ; pests ; predation ; Italy
    Language English
    Dates of publication 2022-0131
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2662247-6
    ISSN 2075-4450
    ISSN 2075-4450
    DOI 10.3390/insects13020158
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Aphrophoridae Role in

    Picciotti, Ugo / Lahbib, Nada / Sefa, Valdete / Porcelli, Francesco / Garganese, Francesca

    Pathogens (Basel, Switzerland)

    2021  Volume 10, Issue 8

    Abstract: ... ...

    Abstract The
    Language English
    Publishing date 2021-08-16
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens10081035
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Effect of Precursors on the Electrochemical Properties of Mixed RuOx/MnOx Electrodes Prepared by Thermal Decomposition.

    Petrucci, Elisabetta / Porcelli, Francesco / Orsini, Monica / De Santis, Serena / Sotgiu, Giovanni

    Materials (Basel, Switzerland)

    2022  Volume 15, Issue 21

    Abstract: Growing thin layers of mixed-metal oxides on titanium supports allows for the preparation of versatile electrodes that can be used in many applications. In this work, electrodes coated with thin films of ruthenium (RuOx) and manganese oxide (MnOx) were ... ...

    Abstract Growing thin layers of mixed-metal oxides on titanium supports allows for the preparation of versatile electrodes that can be used in many applications. In this work, electrodes coated with thin films of ruthenium (RuOx) and manganese oxide (MnOx) were fabricated via thermal decomposition of a precursor solution deposited on a titanium substrate by spin coating. In particular, we combined different Ru and Mn precursors, either organic or inorganic, and investigated their influence on the morphology and electrochemical properties of the materials. The tested salts were: Ruthenium(III) acetylacetonate (Ru(acac)
    Language English
    Publishing date 2022-10-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2487261-1
    ISSN 1996-1944
    ISSN 1996-1944
    DOI 10.3390/ma15217489
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Zelus renardii

    Lahbib, Nada / Picciotti, Ugo / Sefa, Valdete / Boukhris-Bouhachem, Sonia / Porcelli, Francesco / Garganese, Francesca

    Insects

    2022  Volume 13, Issue 2

    Abstract: This study collects data from the literature and updates ... ...

    Abstract This study collects data from the literature and updates our
    Language English
    Publishing date 2022-01-31
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662247-6
    ISSN 2075-4450
    ISSN 2075-4450
    DOI 10.3390/insects13020158
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Electoral accountability and local government efficiency

    Porcelli, Francesco

    Economics of governance Vol. 15, No. 3 , p. 221-251

    quasi-experimental evidence from the Italian health care sector reforms

    2014  Volume 15, Issue 3, Page(s) 221–251

    Author's details Francesco Porcelli
    Keywords Electoral accountability ; DEA ; Decentralisation ; Efficiency ; Health ; IRAP ; Italy ; Panel data ; Stochastic frontier
    Language English
    Size graph. Darst.
    Publisher Springer
    Publishing place Berlin ; Heidelberg
    Document type Article
    ZDB-ID 1491330-6 ; 2011419-9
    ISSN 1435-8131 ; 1435-6104
    ISSN (online) 1435-8131
    ISSN 1435-6104
    Database ECONomics Information System

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  10. Article ; Online: Bioinformatic survey of CRISPR loci across 15 Serratia species.

    Scrascia, Maria / Roberto, Roberta / D'Addabbo, Pietro / Ahmed, Yosra / Porcelli, Francesco / Oliva, Marta / Calia, Carla / Marzella, Angelo / Pazzani, Carlo

    MicrobiologyOpen

    2023  Volume 12, Issue 2, Page(s) e1339

    Abstract: The Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated proteins (CRISPR-Cas) system of prokaryotes is an adaptative immune defense mechanism to protect themselves from invading genetic elements (e.g., phages and plasmids). ... ...

    Abstract The Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated proteins (CRISPR-Cas) system of prokaryotes is an adaptative immune defense mechanism to protect themselves from invading genetic elements (e.g., phages and plasmids). Studies that describe the genetic organization of these prokaryotic systems have mainly reported on the Enterobacteriaceae family (now reorganized within the order of Enterobacterales). For some genera, data on CRISPR-Cas systems remain poor, as in the case of Serratia (now part of the Yersiniaceae family) where data are limited to a few genomes of the species marcescens. This study describes the detection, in silico, of CRISPR loci in 146 Serratia complete genomes and 336 high-quality assemblies available for the species ficaria, fonticola, grimesii, inhibens, liquefaciens, marcescens, nematodiphila, odorifera, oryzae, plymuthica, proteomaculans, quinivorans, rubidaea, symbiotica, and ureilytica. Apart from subtypes I-E and I-F1 which had previously been identified in marcescens, we report that of I-C and the I-E unique locus 1, I-E*, and I-F1 unique locus 1. Analysis of the genomic contexts for CRISPR loci revealed mdtN-phnP as the region mostly shared (grimesii, inhibens, marcescens, nematodiphila, plymuthica, rubidaea, and Serratia sp.). Three new contexts detected in genomes of rubidaea and fonticola (puu genes-mnmA) and rubidaea (osmE-soxG and ampC-yebZ) were also found. The plasmid and/or phage origin of spacers was also established.
    MeSH term(s) Serratia/genetics ; Clustered Regularly Interspaced Short Palindromic Repeats/genetics ; Plasmids/genetics ; Computational Biology ; Genomics
    Language English
    Publishing date 2023-04-26
    Publishing country England
    Document type Journal Article
    ZDB-ID 2661368-2
    ISSN 2045-8827 ; 2045-8827
    ISSN (online) 2045-8827
    ISSN 2045-8827
    DOI 10.1002/mbo3.1339
    Database MEDical Literature Analysis and Retrieval System OnLINE

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