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Article ; Online: Development of innovative antioxidant food packaging systems based on natural extracts from food industry waste and Moringa oleifera leaves.

Barzan, Giulia / Sacco, Alessio / Giovannozzi, Andrea Mario / Portesi, Chiara / Schiavone, Consolato / Salafranca, Jesús / Wrona, Magdalena / Nerín, Cristina / Rossi, Andrea Mario

Food chemistry

2023 Volume 432, Page(s) 137088

Abstract: Active packaging that prolongs food shelf life, maintaining its quality and safety, is an increasing industrial demand, especially if integrated in a circular economy model. In this study, the fabrication and characterization of sustainable cellulose- ... ...

Abstract	Active packaging that prolongs food shelf life, maintaining its quality and safety, is an increasing industrial demand, especially if integrated in a circular economy model. In this study, the fabrication and characterization of sustainable cellulose-based active packaging using food-industry waste and natural extracts as antioxidant agents was assessed. Grape marc, olive pomace and moringa leaf extracts obtained by supercritical fluid, antisolvent and maceration extraction in different solvents were compared for their antioxidant power and phenolic content. Grape and moringa macerates in acetone and methanol, as the most efficient and cost-effective extracts, were incorporated in the packaging as coatings or in-between layers. Both systems showed significant free-radical protection in vitro (antioxidant power 50%) and more than 50% prevention of ground beef lipid peroxidation over 16 days by indirect TBARS and direct in situ Raman microspectroscopy measurements. Therefore, these systems are promising for industrial applications and more sustainable farm-to-fork food production systems.
MeSH term(s)	Cattle ; Animals ; Antioxidants ; Food Packaging ; Moringa oleifera ; Food Additives ; Food ; Industrial Waste
Chemical Substances	Antioxidants ; Food Additives ; Industrial Waste
Language	English
Publishing date	2023-08-09
Publishing country	England
Document type	Journal Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2023.137088
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Article ; Online: Development of innovative antioxidant food packaging systems based on natural extracts from food industry waste and Moringa oleifera leaves

Barzan, Giulia / Sacco, Alessio / Giovannozzi, Andrea Mario / Portesi, Chiara / Schiavone, Consolato / Salafranca, Jesús / Wrona, Magdalena / Nerín, Cristina / Rossi, Andrea Mario

Food Chemistry. 2023 Aug. 09, p.137088-

2023 , Page(s) 137088–

Abstract	Active packaging that prolongs food shelf life, maintaining its quality and safety, is an increasing industrial demand, especially if integrated in a circular economy model. In this study, the fabrication and characterization of sustainable cellulose-based active packaging using food-industry waste and natural extracts as antioxidant agents was assessed. Grape marc, olive pomace and moringa leaf extracts obtained by supercritical fluid, antisolvent and maceration extraction in different solvents were compared for their antioxidant power and phenolic content. Grape and moringa macerates in acetone and methanol, as the most efficient and cost-effective extracts, were incorporated in the packaging as coatings or in-between layers. Both systems showed significant free-radical protection in vitro (antioxidant power 50%) and more than 50% prevention of ground beef lipid peroxidation over 16 days by indirect TBARS and direct in situ Raman microspectroscopy measurements. Therefore, these systems are promising for industrial applications and more sustainable farm-to-fork food production systems.
Keywords	Moringa oleifera ; Raman spectroscopy ; acetone ; antioxidant activity ; antioxidants ; circular economy ; cost effectiveness ; farm to fork ; food chemistry ; food industry ; food processing wastes ; food production ; grape pomace ; grapes ; ground beef ; leaves ; lipid peroxidation ; maceration ; methanol ; olive pomace ; shelf life ; Active packaging ; Natural antioxidants ; Cellulose biopolymer ; Meat shelf life ; Food waste recovery ; Radical scavenging
Language	English
Dates of publication	2023-0809
Publishing place	Elsevier Ltd
Document type	Article ; Online
Note	Pre-press version
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2023.137088
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Article ; Online: [No title information]

Aiuto, Beatrice / Cirrincione, Simona / Giuffrida, Maria Gabriella / Cavallarin, Laura / Portesi, Chiara / Rossi, Andrea Mario / Borreani, Giorgio / Rolla, Giovanni / Geuna, Massimo / Nicola, Stefania / Quinternetto, Anna / Alessi, Lucrezia / Saracco, Elena / Brussino, Luisa / Lamberti, Cristina

Molecular nutrition & food research

2024 , Page(s) e2300796

Abstract: Alpha-gal syndrome (AGS) is a mammalian meat allergy associated with tick bites and specific IgE to the oligosaccharide galactose-α-1,3-galactose (α-gal). Recent studies have shown that 10-20% of AGS patients also react to the dairy proteins. Considering ...

Title translation	Milk Fat Globule Proteins Are Relevant Bovine Milk Allergens in Patients with α‐Gal Syndrome.
Abstract	Alpha-gal syndrome (AGS) is a mammalian meat allergy associated with tick bites and specific IgE to the oligosaccharide galactose-α-1,3-galactose (α-gal). Recent studies have shown that 10-20% of AGS patients also react to the dairy proteins. Considering the already described role of the meat lipid fraction in AGS manifestations, the aim of this work has been to investigate whether the milk fat globule proteins (MFGPs) could be involved in AGS. The MFGPs are extracted and their recognition by the IgE of AGS patients is proved through immunoblotting experiments. The identification of the immunoreactive proteins by LC-HRMS analysis allows to demonstrate for the first time that butyrophillin, lactadherin, and xanthine oxidase (XO) are α-gal glycosylated. The role of xanthine oxidase seems to be prevalent since it is highly recognized by both the anti-α-gal antibody and AGS patient sera. The results obtained in this study provide novel insights in the characterization of α-Gal carrying glycoproteins in bovine milk, supporting the possibility that milk, especially in its whole form, may give reactions in AGS patients. Although additional factors are probably associated with the clinical manifestations, the avoidance of milk and milk products should be considered in individuals with AGS showing symptoms related to milk consumption.
Language	German
Publishing date	2024-05-05
Publishing country	Germany
Document type	English Abstract ; Journal Article
ZDB-ID	2160372-8
ISSN	1613-4133 ; 1613-4125
ISSN (online)	1613-4133
ISSN	1613-4125
DOI	10.1002/mnfr.202300796
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Article: Raman-dielectrophoresis goes viral: towards a rapid and label-free platform for plant virus characterization.

Sacco, Alessio / Barzan, Giulia / Matić, Slavica / Giovannozzi, Andrea M / Rossi, Andrea M / D'Errico, Chiara / Vallino, Marta / Ciuffo, Marina / Noris, Emanuela / Portesi, Chiara

Frontiers in microbiology

2023 Volume 14, Page(s) 1292461

Abstract: An innovative spectroscopic method that allows to chemically and structurally characterize viruses directly in suspension within few minutes was developed. A library of five different plant viruses was obtained combining dielectrophoresis (DEP), ... ...

Abstract	An innovative spectroscopic method that allows to chemically and structurally characterize viruses directly in suspension within few minutes was developed. A library of five different plant viruses was obtained combining dielectrophoresis (DEP), performed with a device specifically designed to capture and agglomerate virus particles, and Raman spectroscopy to provide a chemical fingerprint of virions. The tested viruses, purified from infected plants, were chosen for their economic impact on horticultural crops and for their different morphological and structural features. Using the Raman-DEP device, specific profiles for each virus were successfully obtained, relying on chemical differences occurring even with genetically similar viruses belonging to the same taxonomic species and morphologically indiscernible by transmission electron microscopy (TEM). Moreover, we investigated the potentiality of Raman-DEP to follow dynamic changes occurring upon heat treatment of tobacco mosaic virus (TMV) particles. Raman peak deviations linked to TMV coat protein conformation were observed upon treatment at temperatures equal or higher than 85°C, substantiating the rod-to-spherical shape transitions observed by TEM and the concomitant drastic loss of infectivity following plant inoculation. Overall, the Raman-DEP method can be useful for the characterization of virus (nano)particles, setting the basis to create a database suitable for the study of viruses or virus derived-nanoparticles relevant for the agricultural, medical, or biotechnological fields.
Language	English
Publishing date	2023-11-22
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2023.1292461
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Article: Novel Approaches in Tip-Enhanced Raman Spectroscopy: Accurate Measurement of Enhancement Factors and Pesticide Detection in Tip Dimer Configuration

Sacco, Alessio / Mangino, Silvia / Portesi, Chiara / Vittone, Ettore / Rossi, Andrea Mario

Journal of physical chemistry. 2019 Sept. 16, v. 123, no. 40

2019

Abstract: Tip-enhanced Raman spectroscopy (TERS) allows the precise manipulation of a nanometric probe for surface chemical analysis by plasmon-based amplification of Raman signals; however, acknowledged procedures and materials for assessing the enhancement ... ...

Abstract	Tip-enhanced Raman spectroscopy (TERS) allows the precise manipulation of a nanometric probe for surface chemical analysis by plasmon-based amplification of Raman signals; however, acknowledged procedures and materials for assessing the enhancement factor in different configurations are still lacking. In this work, we propose a technique for the standardization of TERS intensity measurements, by chemisorption of different organic Raman-active molecules on plasmonic probes, and compare it to the conventional procedures addressed to the same goals. In addition, by ideally considering TERS as a special case of surface-enhanced Raman (SERS) involving a single nanoparticle, we experimentally realized the three most common configurations in SERS: i. isolated particle, ii. single scattering probe on a surface, and iii. nanoparticle dimers. To achieve the latter in an accessible way with plasmonic probes, we established a fabrication procedure for a substrate presenting multiple nanometric tips, intended to be easily approached and mapped for fast, reproducible assembly of tunable tip–tip dimers that further amplify Raman scattering with respect to conventional gap mode TERS; enhancement factors up to (1.4 ± 0.4) × 10¹⁰ were calculated. The three configurations were successfully tested, and enhancement factors were quantified with their associated uncertainties, employing self-assembled monolayers of several probe molecules, including thiophenol, a de facto enhanced Raman standard, and thiram, a common use, law-regulated pesticide, hence opening up TERS to real world applications in agricultural and food analysis.
Keywords	Raman spectroscopy ; chemical analysis ; food analysis ; nanoparticles ; physical chemistry ; thiram ; uncertainty
Language	English
Dates of publication	2019-0916
Size	p. 24723-24730.
Publishing place	American Chemical Society
Document type	Article
ISSN	1932-7455
DOI	10.1021/acs.jpcc.9b07016
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Surface Minimal Bactericidal Concentration: A comparative study of active glasses functionalized with different-sized silver nanoparticles.

Barzan, Giulia / Rocchetti, Luca / Portesi, Chiara / Pellegrino, Francesco / Taglietti, Angelo / Rossi, Andrea Mario / Giovannozzi, Andrea Mario

Colloids and surfaces. B, Biointerfaces

2021 Volume 204, Page(s) 111800

Abstract: In this work the quantification of antimicrobial properties of differently sized AgNPs immobilized on a surface was studied. Three different sizes of spheroidal AgNPs with a diameter of (6, 30 and 52) nm were synthetized and characterized with UV-vis, ... ...

Abstract	In this work the quantification of antimicrobial properties of differently sized AgNPs immobilized on a surface was studied. Three different sizes of spheroidal AgNPs with a diameter of (6, 30 and 52) nm were synthetized and characterized with UV-vis, SEM, TEM and ICP-MS. The MIC (Minimal Inhibitory Concentration) and MBC (Minimal Bactericidal Concentration) against Escherichia coli were investigated. Then, the antibacterial efficacy (R) of amino-silanized glasses coated with different amounts of the three sizes of AgNPs were quantified by international standard ISO 22196 adapted protocol against E. coli, clarifying the relationship between size and antibacterial properties of immobilized AgNPs on a surface. The total amount of silver present on glasses with an R ∼ 6 for each AgNPs size was quantified with ICP-MS and this was considered the Surface MBC (SMBC), which were found to be (0.023, 0.026 and 0.034) μg/cm
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Escherichia coli ; Metal Nanoparticles ; Microbial Sensitivity Tests ; Silver/pharmacology
Chemical Substances	Anti-Bacterial Agents ; Silver (3M4G523W1G)
Language	English
Publishing date	2021-04-29
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500523-9
ISSN	1873-4367 ; 0927-7765
ISSN (online)	1873-4367
ISSN	0927-7765
DOI	10.1016/j.colsurfb.2021.111800
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Article ; Online: Development of a candidate reference sample for the characterization of tip-enhanced Raman spectroscopy spatial resolution.

Sacco, Alessio / Imbraguglio, Dario / Giovannozzi, Andrea M / Portesi, Chiara / Rossi, Andrea M

RSC advances

2018 Volume 8, Issue 49, Page(s) 27863–27869

Abstract: Tip-Enhanced Raman Spectroscopy (TERS) is a topographic and chemical analysis technique with nanoscale resolution, consisting of the combination of Scanning Probe Microscopy (SPM) and Localized Surface Plasmon Resonance (LSPR) for the enhancement of ... ...

Abstract	Tip-Enhanced Raman Spectroscopy (TERS) is a topographic and chemical analysis technique with nanoscale resolution, consisting of the combination of Scanning Probe Microscopy (SPM) and Localized Surface Plasmon Resonance (LSPR) for the enhancement of Raman scattering in the vicinity of the probe. The quantification of spatial resolution represents an important issue, and, as of now, standards for calibration are not available. In the present work a candidate reference sample for TERS measurements was fabricated. It consists of a flat, conductive gold surface with a nanometric grating of a self-assembled monolayer of Raman-active organic molecules fabricated by an optimized Electron Beam Lithography (EBL) method to replicate established SPM calibration standards. Its feasibility as a TERS standard was tested by STM-TERS imaging.
Language	English
Publishing date	2018-08-03
Publishing country	England
Document type	Journal Article
ISSN	2046-2069
ISSN (online)	2046-2069
DOI	10.1039/c8ra03762k
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Article ; Online: Aluminum-Titanium Bilayer for Near-Infrared Transition Edge Sensors.

Lolli, Lapo / Taralli, Emanuele / Portesi, Chiara / Rajteri, Mauro / Monticone, Eugenio

Sensors (Basel, Switzerland)

2016 Volume 16, Issue 7

Abstract: Transition-edge sensors (TESs) are single photon detectors attractive for applications in quantum optics and quantum information experiments owing to their photon number resolving capability. Nowadays, high-energy resolution TESs for telecommunication ... ...

Abstract	Transition-edge sensors (TESs) are single photon detectors attractive for applications in quantum optics and quantum information experiments owing to their photon number resolving capability. Nowadays, high-energy resolution TESs for telecommunication are based on either W or Au/Ti films, demonstrating slow recovery time constants. We report our progress on the development of an Al/Ti TES. Since bulk aluminum has a critical temperature (Tc) of ca. 1.2 K and a sufficiently low specific heat (less than 10(-4) J/cm³K²), it can be employed to produce the sensitive material for optical TESs. Furthermore, exploiting its high Tc, Al-based TESs can be trimmed in a wider temperature range with respect to Ti or W. A first Al/Ti TES with a Tc ≈ 142 mK, investigated from a thermal and optical point of view, has shown a response time constant of about 2 μs and single photon discrimination with 0.34 eV energy resolution at telecom wavelength, demonstrating that Al/Ti films are suitable to produce TESs for visible and NIR photon counting.
Language	English
Publishing date	2016-06-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s16070953
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Article ; Online: Influence of the long-range ordering of gold-coated Si nanowires on SERS.

Cara, Eleonora / Mandrile, Luisa / Ferrarese Lupi, Federico / Giovannozzi, Andrea Mario / Dialameh, Masoud / Portesi, Chiara / Sparnacci, Katia / De Leo, Natascia / Rossi, Andrea Mario / Boarino, Luca

Scientific reports

2018 Volume 8, Issue 1, Page(s) 11305

Abstract: Controlling the location and the distribution of hot spots is a crucial aspect in the fabrication of surface-enhanced Raman spectroscopy (SERS) substrates for bio-analytical applications. The choice of a suitable method to tailor the dimensions and the ... ...

Abstract	Controlling the location and the distribution of hot spots is a crucial aspect in the fabrication of surface-enhanced Raman spectroscopy (SERS) substrates for bio-analytical applications. The choice of a suitable method to tailor the dimensions and the position of plasmonic nanostructures becomes fundamental to provide SERS substrates with significant signal enhancement, homogeneity and reproducibility. In the present work, we studied the influence of the long-range ordering of different flexible gold-coated Si nanowires arrays on the SERS activity. The substrates are made by nanosphere lithography and metal-assisted chemical etching. The degree of order is quantitatively evaluated through the correlation length (ξ) as a function of the nanosphere spin-coating speed. Our findings showed a linear increase of the SERS signal for increasing values of ξ, coherently with a more ordered and dense distribution of hot spots on the surface. The substrate with the largest ξ of 1100 nm showed an enhancement factor of 2.6 · 10
Language	English
Publishing date	2018-07-27
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-018-29641-x
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Article ; Online: A calibration procedure for a traceable contamination analysis on medical devices by combined X-ray spectrometry and ambient spectroscopic techniques.

Pollakowski-Herrmann, Beatrix / Hornemann, Andrea / Giovannozzi, Andrea M / Green, Felicia / Gunning, Paul / Portesi, Chiara / Rossi, Andrea / Seim, Christian / Steven, Rory / Tyler, Bonnie / Beckhoff, Burkhard

Journal of pharmaceutical and biomedical analysis

2018 Volume 150, Page(s) 308–317

Abstract: There is a strong need in the medical device industry to decrease failure rates of biomedical devices by reducing the incidence of defect structures and contaminants during the production process. The detection and identification of defect structures and ...

Abstract	There is a strong need in the medical device industry to decrease failure rates of biomedical devices by reducing the incidence of defect structures and contaminants during the production process. The detection and identification of defect structures and contaminants is crucial for many industrial applications. The present study exploits reference-free X-ray fluorescence (XRF) analysis as an analytical tool for the traceable characterization of surface contaminants of medical devices, in particular N,N'-ethylene-bis (stearamide), an ubiquitous compound used in many industrial applications as a release agent or friction reduction additive. Reference-free XRF analysis as primary method has been proven to be capable of underpinning all other applied methods since it yields the absolute mass deposition of the selected N,N'-ethylene-bis (stearamide) contaminant whilst X-ray absorption fine structure analysis determines the chemical species. Ambient vibrational spectroscopy and mass spectroscopy methodologies such as Fourier transform infrared, Raman, and secondary ion mass spectroscopy have been used in this systematic procedure providing an extensive range of complementary analyses. The calibration procedure described in this paper was developed using specially designed and fabricated model systems varying in thickness and substrate material. Furthermore, typical real medical devices such as both a polyethylene hip liner and a silver-coated wound dressing have been contaminated and investigated by these diverse methods, enabling testing of this developed procedure. These well-characterized samples may be used as calibration standards for bench top instrumentation from the perspective of providing traceable analysis of biomaterials and surface treatments. These findings demonstrate the potential importance and usefulness of combining complementary methods for a better understanding of the relevant organic materials.
MeSH term(s)	Calibration ; Equipment Contamination ; Equipment and Supplies ; Reference Standards ; Reproducibility of Results ; Spectrometry, Mass, Secondary Ion ; Spectrometry, X-Ray Emission/standards ; Spectroscopy, Fourier Transform Infrared ; Spectrum Analysis, Raman ; Stearic Acids/analysis
Chemical Substances	Stearic Acids
Language	English
Publishing date	2018-02-20
Publishing country	England
Document type	Journal Article
ZDB-ID	604917-5
ISSN	1873-264X ; 0731-7085
ISSN (online)	1873-264X
ISSN	0731-7085
DOI	10.1016/j.jpba.2017.12.007
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