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  1. Article ; Online: The breathtaking world of human respiratory in vitro models: Investigating lung diseases and infections in 3D models, organoids, and lung-on-chip.

    Dichtl, Stefanie / Posch, Wilfried / Wilflingseder, Doris

    European journal of immunology

    2024  Volume 54, Issue 3, Page(s) e2250356

    Abstract: The COVID-19 pandemic illustrated an urgent need for sophisticated, human tissue models to rapidly test and develop effective treatment options against this newly emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Thus, in particular, ...

    Abstract The COVID-19 pandemic illustrated an urgent need for sophisticated, human tissue models to rapidly test and develop effective treatment options against this newly emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Thus, in particular, the last 3 years faced an extensive boost in respiratory and pulmonary model development. Nowadays, 3D models, organoids and lung-on-chip, respiratory models in perfusion, or precision-cut lung slices are used to study complex research questions in human primary cells. These models provide physiologically relevant systems for studying SARS-CoV-2 and, of course, other respiratory pathogens, but they are, too, suited for studying lung pathologies, such as CF, chronic obstructive pulmonary disease, or asthma, in more detail in terms of viral infection. With these models, the cornerstone has been laid for further advancing the organs by, for example, inclusion of several immune cell types or humoral immune components, combination with other organs in microfluidic organ-on-chip devices, standardization and harmonization of the devices for reliable and reproducible drug and vaccine testing in high throughput.
    MeSH term(s) Humans ; Pandemics ; Lung/pathology ; COVID-19/pathology ; SARS-CoV-2 ; Organoids
    Language English
    Publishing date 2024-02-15
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 120108-6
    ISSN 1521-4141 ; 0014-2980
    ISSN (online) 1521-4141
    ISSN 0014-2980
    DOI 10.1002/eji.202250356
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 489: Show issues Location:
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  2. Article: [No title information]

    Wilflingseder, Doris / Posch, Wilfried

    Biospektrum : Zeitschrift der Gesellschaft fur Biologishe Chemie (GBCH) und der Vereinigung fur Allgemeine und Angewandte Mikrobiologie (VAAM)

    2022  Volume 28, Issue 1, Page(s) 43–46

    Abstract: Excessive inflammation triggered by a hitherto undescribed mechanism is a hallmark of severe SARS-CoV-2 infection and is associated with enhanced pathogenicity and mortality. Complement hyper activation promotes lung injury and was observed in patients ... ...

    Title translation Wie sich COVID-19 in der 3D-Zellkultur simulieren lässt.
    Abstract Excessive inflammation triggered by a hitherto undescribed mechanism is a hallmark of severe SARS-CoV-2 infection and is associated with enhanced pathogenicity and mortality. Complement hyper activation promotes lung injury and was observed in patients suffering from MERS-CoV, SARS-CoV-1 and SARS-CoV-2 infections. To evaluate the very first interactions of SARS-CoV-2 patient isolates with human epithelial tissues, 3D models of the human respiratory tract as well as lung organoids are highly suitable.
    Language German
    Publishing date 2022-02-13
    Publishing country Germany
    Document type English Abstract ; Journal Article ; Review
    ZDB-ID 2203536-9
    ISSN 1868-6249 ; 0947-0867
    ISSN (online) 1868-6249
    ISSN 0947-0867
    DOI 10.1007/s12268-022-1712-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: HIV-1

    Bertacchi, Giulia / Posch, Wilfried / Wilflingseder, Doris

    Biomolecules

    2022  Volume 12, Issue 2

    Abstract: Nonadjacent immune cells communicate through a complex network of tunneling nanotubes (TNTs). TNTs can be hijacked by HIV-1, allowing it to spread between connected cells. Dendritic cells (DCs) are among the first cells to encounter HIV-1 at mucosal ... ...

    Abstract Nonadjacent immune cells communicate through a complex network of tunneling nanotubes (TNTs). TNTs can be hijacked by HIV-1, allowing it to spread between connected cells. Dendritic cells (DCs) are among the first cells to encounter HIV-1 at mucosal sites, but they are usually efficiently infected only at low levels. However, HIV-1 was demonstrated to productively infect DCs when the virus was complement-opsonized (HIV-C). Such HIV-C-exposed DCs mediated an improved antiviral and T-cell stimulatory capacity. The role of TNTs in combination with complement in enhancing DC infection with HIV-C remains to be addressed. To this aim, we evaluated TNT formation on the surface of DCs or DC/CD4
    MeSH term(s) Cell Communication ; Complement System Proteins/metabolism ; Dendritic Cells ; HIV Infections ; HIV-1/metabolism ; Humans ; Receptor, Anaphylatoxin C5a/therapeutic use ; T-Lymphocytes
    Chemical Substances C5AR1 protein, human ; Receptor, Anaphylatoxin C5a ; Complement System Proteins (9007-36-7)
    Language English
    Publishing date 2022-02-15
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom12020313
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Evaluation of Inoculum Preparation for Etest and EUCAST Broth Dilution to Detect Anidulafungin Polyresistance in Candida glabrata.

    Knoll, Miriam Alisa / Samardzic, Eldina / Posch, Wilfried / Lass-Flörl, Cornelia

    Antimicrobial agents and chemotherapy

    2022  Volume 66, Issue 8, Page(s) e0016822

    Abstract: The influence of inoculum preparation in EUCAST broth dilution and Etest to detect the coexistence of resistant and ... ...

    Abstract The influence of inoculum preparation in EUCAST broth dilution and Etest to detect the coexistence of resistant and susceptible
    MeSH term(s) Anidulafungin/pharmacology ; Antifungal Agents/pharmacology ; Candida ; Candida glabrata ; Disk Diffusion Antimicrobial Tests ; Microbial Sensitivity Tests ; Reproducibility of Results
    Chemical Substances Antifungal Agents ; Anidulafungin (9HLM53094I)
    Language English
    Publishing date 2022-07-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 217602-6
    ISSN 1098-6596 ; 0066-4804
    ISSN (online) 1098-6596
    ISSN 0066-4804
    DOI 10.1128/aac.00168-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 809: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Einzelsignatur: Show issues

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  5. Article: Microbiological and Molecular Diagnosis of Mucormycosis: From Old to New.

    Lackner, Nina / Posch, Wilfried / Lass-Flörl, Cornelia

    Microorganisms

    2021  Volume 9, Issue 7

    Abstract: Members of the order Mucorales may cause severe invasive fungal infections (mucormycosis) in immune-compromised and otherwise ill patients. Diagnosis of Mucorales infections and discrimination from other filamentous fungi are crucial for correct ... ...

    Abstract Members of the order Mucorales may cause severe invasive fungal infections (mucormycosis) in immune-compromised and otherwise ill patients. Diagnosis of Mucorales infections and discrimination from other filamentous fungi are crucial for correct management. Here, we present an overview of current state-of-the-art mucormycosis diagnoses, with a focus on recent developments in the molecular field. Classical diagnostic methods comprise histology/microscopy as well as culture and are still the gold standard. Newer molecular methods are evolving quickly and display great potential in early diagnosis, although standardization is still missing. Among them, quantitative PCR assays with or without melt curve analysis are most widely used to detect fungal DNA in clinical samples. Depending on the respective assay, sequencing of the resulting PCR product can be necessary for genus or even species identification. Further, DNA-based methods include microarrays and PCR-ESI-MS. However, general laboratory standards are still in development, meaning that molecular methods are currently limited to add-on analytics to culture and microscopy.
    Language English
    Publishing date 2021-07-16
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9071518
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: P80 natural essence spray and lozenges provide respiratory protection against Influenza A, B, and SARS-CoV-2.

    Zaderer, Viktoria / Diem, Gabriel / Posch, Wilfried / Jakschitz, Thomas / Bonn, Günther K / Bellmann-Weiler, Rosa / Huber, Lukas A / Wilflingseder, Doris

    Respiratory research

    2024  Volume 25, Issue 1, Page(s) 102

    Abstract: Seasonally circulating viruses, such as Influenza, as well as newly emerging viruses and variants thereof, and waning immunity urge the need for safe, easy-to-use and inexpensive drugs to protect from these challenges. To prevent transmission of these ... ...

    Abstract Seasonally circulating viruses, such as Influenza, as well as newly emerging viruses and variants thereof, and waning immunity urge the need for safe, easy-to-use and inexpensive drugs to protect from these challenges. To prevent transmission of these viruses and subsequent excessive inflammatory reactions on mucous membranes, we tested the efficacy of the natural essence P80 as spray and in form of lozenges against respiratory infections caused by SARS-CoV-2 variants of concern (VoCs), influenza A (H3N2) and influenza B (Victoria). P80 natural essence, a Dimocarpus longan extract, shielded highly differentiated human airway epithelia from SARS-CoV-2 wildtype and Omicron variant as well as Influenza A and B infection and dampened inflammation by down-modulating pro-inflammatory cytokine and anaphylatoxin secretion. A single application of P80 natural essence spray maintained tissue integrity long-term. This also significantly reduced the release of infectious viral particles and the secretion of IP10, MCP1, RANTES and C3a, all of which mediate the migration of immune cells to the sites of infection. Even P80 lozenges dissolved in distilled water or non-neutralizing saliva efficiently prevented SARS-CoV-2 and Influenza-induced tissue destruction. Consequently, our in vitro data suggest that P80 natural essence can act as antiviral prophylactic, both in form of nasal or oral spray and in form of lozenges, independent of circulating respiratory challenges.
    MeSH term(s) Humans ; Influenza, Human/prevention & control ; Influenza A Virus, H3N2 Subtype ; SARS-CoV-2 ; COVID-19 ; Inflammation
    Language English
    Publishing date 2024-02-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 2041675-1
    ISSN 1465-993X ; 1465-993X
    ISSN (online) 1465-993X
    ISSN 1465-993X
    DOI 10.1186/s12931-024-02718-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: SARS-CoV-2-infected primary human airway epithelia illustrate mucus hypersecretion.

    Posch, Wilfried / Lass-Flörl, Cornelia / Wilflingseder, Doris

    The Journal of allergy and clinical immunology

    2021  Volume 148, Issue 3, Page(s) 909

    MeSH term(s) COVID-19 ; Humans ; Mucus ; Respiratory System ; SARS-CoV-2
    Language English
    Publishing date 2021-07-17
    Publishing country United States
    Document type Editorial ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Comment
    ZDB-ID 121011-7
    ISSN 1097-6825 ; 1085-8725 ; 0091-6749
    ISSN (online) 1097-6825 ; 1085-8725
    ISSN 0091-6749
    DOI 10.1016/j.jaci.2021.05.047
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    In stock of ZB MED Cologne/Königswinter

    Uh III Zs.92: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  8. Article ; Online: Correction: ColdZyme® protects airway epithelia from infection with BA.4/5.

    Zaderer, Viktoria / Dichtl, Stefanie / Bellmann-Weiler, Rosa / Lass-Flörl, Cornelia / Posch, Wilfried / Wilflingseder, Doris

    Respiratory research

    2023  Volume 24, Issue 1, Page(s) 27

    Language English
    Publishing date 2023-01-24
    Publishing country England
    Document type Published Erratum
    ZDB-ID 2041675-1
    ISSN 1465-993X ; 1465-993X
    ISSN (online) 1465-993X
    ISSN 1465-993X
    DOI 10.1186/s12931-023-02326-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Microbiological and Molecular Diagnosis of Mucormycosis: From Old to New

    Lackner, Nina / Posch, Wilfried / Lass-Flörl, Cornelia

    Microorganisms. 2021 July 16, v. 9, no. 7

    2021  

    Abstract: Members of the order Mucorales may cause severe invasive fungal infections (mucormycosis) in immune-compromised and otherwise ill patients. Diagnosis of Mucorales infections and discrimination from other filamentous fungi are crucial for correct ... ...

    Abstract Members of the order Mucorales may cause severe invasive fungal infections (mucormycosis) in immune-compromised and otherwise ill patients. Diagnosis of Mucorales infections and discrimination from other filamentous fungi are crucial for correct management. Here, we present an overview of current state-of-the-art mucormycosis diagnoses, with a focus on recent developments in the molecular field. Classical diagnostic methods comprise histology/microscopy as well as culture and are still the gold standard. Newer molecular methods are evolving quickly and display great potential in early diagnosis, although standardization is still missing. Among them, quantitative PCR assays with or without melt curve analysis are most widely used to detect fungal DNA in clinical samples. Depending on the respective assay, sequencing of the resulting PCR product can be necessary for genus or even species identification. Further, DNA-based methods include microarrays and PCR-ESI-MS. However, general laboratory standards are still in development, meaning that molecular methods are currently limited to add-on analytics to culture and microscopy.
    Keywords DNA ; Mucorales ; early diagnosis ; fungi ; histology ; microarray technology ; microscopy ; quantitative polymerase chain reaction ; species identification ; zygomycosis
    Language English
    Dates of publication 2021-0716
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9071518
    Database NAL-Catalogue (AGRICOLA)

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  10. Article ; Online: ColdZyme® protects airway epithelia from infection with BA.4/5.

    Zaderer, Viktoria / Dichtl, Stefanie / Bellmann Weiler, Rosa / Lass Flörl, Cornelia / Posch, Wilfried / Wilflingseder, Doris

    Respiratory research

    2022  Volume 23, Issue 1, Page(s) 300

    Abstract: Vaccines against SARS-CoV-2 protect from critical or severe pathogenesis also against new variants of concern (VOCs) such as BA.4 and BA.5, but immediate interventions to avoid viral transmission and subsequent inflammatory reactions are needed. Here we ... ...

    Abstract Vaccines against SARS-CoV-2 protect from critical or severe pathogenesis also against new variants of concern (VOCs) such as BA.4 and BA.5, but immediate interventions to avoid viral transmission and subsequent inflammatory reactions are needed. Here we applied the ColdZyme® medical device mouth spray to fully differentiated, polarized human epithelium cultured at an air-liquid interphase (ALI). We found using VOCs BA.1 and BA.4/5 that this device effectively blocked respiratory tissue infection. While infection with these VOCs resulted in intracellular complement activation, thus enhanced inflammation, and drop of transepithelial resistance, these phenomena were prevented by a single administration of this medical device. Thus, ColdZyme® mouth spray significantly shields epithelial integrity, hinders virus infection and blocks in a secondary effect intrinsic complement activation within airway cultures also in terms of the highly contagious VOCs BA.4/5. Crucially, our in vitro data suggest that ColdZyme® mouth spray may have an impact to protect against SARS-CoV-2 transmission, also in case of the Omicron BA.1, BA.4 and BA.5 variants.
    MeSH term(s) Humans ; Epithelial Cells ; COVID-19 Vaccines ; SARS-CoV-2 ; COVID-19 ; Epithelium ; Respiratory Tract Infections/prevention & control
    Chemical Substances COVID-19 Vaccines
    Language English
    Publishing date 2022-10-31
    Publishing country England
    Document type Letter
    ZDB-ID 2041675-1
    ISSN 1465-993X ; 1465-993X
    ISSN (online) 1465-993X
    ISSN 1465-993X
    DOI 10.1186/s12931-022-02223-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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