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  1. Article ; Online: Using Rapid Prototyping to Develop a Cell-Based Platform with Electrical Impedance Sensor Membranes for In Vitro RPMI2650 Nasal Nanotoxicology Monitoring.

    Vasconez Martinez, Mateo Gabriel / Reihs, Eva I / Stuetz, Helene M / Hafner, Astrid / Brandauer, Konstanze / Selinger, Florian / Schuller, Patrick / Bastus, Neus / Puntes, Victor / Frank, Johannes / Tomischko, Wolfgang / Frauenlob, Martin / Ertl, Peter / Resch, Christian / Bauer, Gerald / Povoden, Guenter / Rothbauer, Mario

    Biosensors

    2024  Volume 14, Issue 2

    Abstract: Due to advances in additive manufacturing and prototyping, affordable and rapid microfluidic sensor-integrated assays can be fabricated using additive manufacturing, xurography and electrode shadow masking to create versatile platform technologies aimed ... ...

    Abstract Due to advances in additive manufacturing and prototyping, affordable and rapid microfluidic sensor-integrated assays can be fabricated using additive manufacturing, xurography and electrode shadow masking to create versatile platform technologies aimed toward qualitative assessment of acute cytotoxic or cytolytic events using stand-alone biochip platforms in the context of environmental risk assessment. In the current study, we established a nasal mucosa biosensing platform using RPMI2650 mucosa cells inside a membrane-integrated impedance-sensing biochip using exclusively rapid prototyping technologies. In a final proof-of-concept, we applied this biosensing platform to create human cell models of nasal mucosa for monitoring the acute cytotoxic effect of zinc oxide reference nanoparticles. Our data generated with the biochip platform successfully monitored the acute toxicity and cytolytic activity of 6 mM zinc oxide nanoparticles, which was non-invasively monitored as a negative impedance slope on nasal epithelial models, demonstrating the feasibility of rapid prototyping technologies such as additive manufacturing and xurography for cell-based platform development.
    MeSH term(s) Humans ; Electric Impedance ; Zinc Oxide ; Microfluidics ; Biosensing Techniques
    Chemical Substances Zinc Oxide (SOI2LOH54Z)
    Language English
    Publishing date 2024-02-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662125-3
    ISSN 2079-6374 ; 2079-6374
    ISSN (online) 2079-6374
    ISSN 2079-6374
    DOI 10.3390/bios14020107
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: The Cultivation Modality and Barrier Maturity Modulate the Toxicity of Industrial Zinc Oxide and Titanium Dioxide Nanoparticles on Nasal, Buccal, Bronchial, and Alveolar Mucosa Cell-Derived Barrier Models.

    Stuetz, Helene / Reihs, Eva I / Neuhaus, Winfried / Pflüger, Maren / Hundsberger, Harald / Ertl, Peter / Resch, Christian / Bauer, Gerald / Povoden, Günter / Rothbauer, Mario

    International journal of molecular sciences

    2023  Volume 24, Issue 6

    Abstract: As common industrial by-products, airborne engineered nanomaterials are considered important environmental toxins to monitor due to their potential health risks to humans and animals. The main uptake routes of airborne nanoparticles are nasal and/or oral ...

    Abstract As common industrial by-products, airborne engineered nanomaterials are considered important environmental toxins to monitor due to their potential health risks to humans and animals. The main uptake routes of airborne nanoparticles are nasal and/or oral inhalation, which are known to enable the transfer of nanomaterials into the bloodstream resulting in the rapid distribution throughout the human body. Consequently, mucosal barriers present in the nose, buccal, and lung have been identified and intensively studied as the key tissue barrier to nanoparticle translocation. Despite decades of research, surprisingly little is known about the differences among various mucosa tissue types to tolerate nanoparticle exposures. One limitation in comparing nanotoxicological data sets can be linked to a lack of harmonization and standardization of cell-based assays, where (a) different cultivation conditions such as an air-liquid interface or submerged cultures, (b) varying barrier maturity, and (c) diverse media substitutes have been used. The current comparative nanotoxicological study, therefore, aims at analyzing the toxic effects of nanomaterials on four human mucosa barrier models including nasal (RPMI2650), buccal (TR146), alveolar (A549), and bronchial (Calu-3) mucosal cell lines to better understand the modulating effects of tissue maturity, cultivation conditions, and tissue type using standard transwell cultivations at liquid-liquid and air-liquid interfaces. Overall, cell size, confluency, tight junction localization, and cell viability as well as barrier formation using 50% and 100% confluency was monitored using trans-epithelial-electrical resistance (TEER) measurements and resazurin-based Presto Blue assays of immature (e.g., 5 days) and mature (e.g., 22 days) cultures in the presence and absence of corticosteroids such as hydrocortisone. Results of our study show that cellular viability in response to increasing nanoparticle exposure scenarios is highly compound and cell-type specific (TR146 6 ± 0.7% at 2 mM ZnO (ZnO) vs. ~90% at 2 mM TiO
    MeSH term(s) Animals ; Humans ; Zinc Oxide/toxicity ; Nanoparticles/toxicity ; Titanium/toxicity ; Mucous Membrane
    Chemical Substances Zinc Oxide (SOI2LOH54Z) ; titanium dioxide (15FIX9V2JP) ; Titanium (D1JT611TNE)
    Language English
    Publishing date 2023-03-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24065634
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: The Multi-Gas Sensor for Remote UAV and UGV Missions-Development and Tests.

    Kaliszewski, Miron / Włodarski, Maksymilian / Młyńczak, Jarosław / Jankiewicz, Bartłomiej / Auer, Lukas / Bartosewicz, Bartosz / Liszewska, Malwina / Budner, Bogusław / Szala, Mateusz / Schneider, Bernhard / Povoden, Günter / Kopczyński, Krzysztof

    Sensors (Basel, Switzerland)

    2021  Volume 21, Issue 22

    Abstract: In this article, we present a versatile gas detector that can operate on an unmanned aerial vehicle (UAV) or unmanned ground vehicle (UGV). The device has six electrochemical modules, which can be selected to measure specific gases, according to the ... ...

    Abstract In this article, we present a versatile gas detector that can operate on an unmanned aerial vehicle (UAV) or unmanned ground vehicle (UGV). The device has six electrochemical modules, which can be selected to measure specific gases, according to the mission requirements. The gas intake is realized by a miniaturized vacuum pump, which provides immediate gas distribution to the sensors and improves a fast response. The measurement data are sent wirelessly to the operator's computer, which continuously stores results and presents them in real time. The 2 m tubing allows measurements to be taken in places that are not directly accessible to the UGV or the UAV. While UAVs significantly enhanced the versatility of sensing applications, point gas detection is challenging due to the downwash effect and gas dilution produced by the rotors. In our work, we demonstrated the method of downwash effect reduction at aerial point gas measurements by applying a long-distance probe, which was kept between the UAV and the examined object. Moreover, we developed a safety connection protecting the UAV and sensor in case of accidental jamming of the tubing inside the examined cavity. The methods presented provide an effective gas metering strategy using UAVs.
    Language English
    Publishing date 2021-11-16
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2052857-7
    ISSN 1424-8220 ; 1424-8220
    ISSN (online) 1424-8220
    ISSN 1424-8220
    DOI 10.3390/s21227608
    Database MEDical Literature Analysis and Retrieval System OnLINE

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