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  1. Article: NEKL-4 regulates microtubule stability and mitochondrial health in

    Power, Kaiden M / Nguyen, Ken C / Silva, Andriele / Singh, Shaneen / Hall, David H / Rongo, Christopher / Barr, Maureen M

    bioRxiv : the preprint server for biology

    2024  

    Abstract: Ciliopathies are often caused by defects in the ciliary microtubule core. Glutamylation is abundant in cilia, and its dysregulation may contribute to ciliopathies and neurodegeneration. Mutation of the deglutamylase CCP1 causes infantile-onset ... ...

    Abstract Ciliopathies are often caused by defects in the ciliary microtubule core. Glutamylation is abundant in cilia, and its dysregulation may contribute to ciliopathies and neurodegeneration. Mutation of the deglutamylase CCP1 causes infantile-onset neurodegeneration. In
    Language English
    Publishing date 2024-02-14
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.02.14.580304
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Ciliary intrinsic mechanisms regulate dynamic ciliary extracellular vesicle release from sensory neurons.

    Wang, Juan / Saul, Josh / Nikonorova, Inna A / Cruz, Carlos Nava / Power, Kaiden M / Nguyen, Ken C / Hall, David H / Barr, Maureen M

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Cilia-derived extracellular vesicles (EVs) contain signaling proteins and act in intercellular communication. Polycystin-2 (PKD-2), a transient receptor potential channel, is a conserved ciliary EVs cargo. ...

    Abstract Cilia-derived extracellular vesicles (EVs) contain signaling proteins and act in intercellular communication. Polycystin-2 (PKD-2), a transient receptor potential channel, is a conserved ciliary EVs cargo.
    Language English
    Publishing date 2023-11-03
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.11.01.565151
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Isolation, profiling, and tracking of extracellular vesicle cargo in Caenorhabditis elegans.

    Nikonorova, Inna A / Wang, Juan / Cope, Alexander L / Tilton, Peter E / Power, Kaiden M / Walsh, Jonathon D / Akella, Jyothi S / Krauchunas, Amber R / Shah, Premal / Barr, Maureen M

    Current biology : CB

    2022  Volume 32, Issue 9, Page(s) 1924–1936.e6

    Abstract: Extracellular vesicles (EVs) may mediate intercellular communication by carrying protein and RNA cargo. The composition, biology, and roles of EVs in physiology and pathology have been primarily studied in the context of biofluids and in cultured ... ...

    Abstract Extracellular vesicles (EVs) may mediate intercellular communication by carrying protein and RNA cargo. The composition, biology, and roles of EVs in physiology and pathology have been primarily studied in the context of biofluids and in cultured mammalian cells. The experimental tractability of C. elegans makes for a powerful in vivo animal system to identify and study EV cargo from its cellular source. We developed an innovative method to label, track, and profile EVs using genetically encoded, fluorescent-tagged EV cargo and conducted a large-scale isolation and proteomic profiling. Nucleic acid binding proteins (∼200) are overrepresented in our dataset. By integrating our EV proteomic dataset with single-cell transcriptomic data, we identified and validated ciliary EV cargo: CD9-like tetraspanin (TSP-6), ectonucleotide pyrophosphatase/phosphodiesterase (ENPP-1), minichromosome maintenance protein (MCM-3), and double-stranded RNA transporter SID-2. C. elegans EVs also harbor RNA, suggesting that EVs may play a role in extracellular RNA-based communication.
    MeSH term(s) Animals ; Caenorhabditis elegans/genetics ; Cell Communication ; Extracellular Vesicles/metabolism ; Mammals/genetics ; Proteomics ; RNA
    Chemical Substances RNA (63231-63-0)
    Language English
    Publishing date 2022-03-24
    Publishing country England
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 1071731-6
    ISSN 1879-0445 ; 0960-9822
    ISSN (online) 1879-0445
    ISSN 0960-9822
    DOI 10.1016/j.cub.2022.03.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3208: Show issues Location:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
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  4. Article ; Online: Interactions between the WEE-1.3 kinase and the PAM-1 aminopeptidase in oocyte maturation and the early C. elegans embryo.

    Benton, Dorothy / Jaeger, Eva C / Kilner, Arielle / Kimble, Ashley / Lowry, Josh / Schleicher, Emily M / Power, Kaiden M / Uibel, Danielle / Eisele, Caprice / Bowerman, Bruce / Lyczak, Rebecca

    G3 (Bethesda, Md.)

    2021  Volume 11, Issue 4

    Abstract: Puromycin-sensitive aminopeptidases are found across phyla and are known to regulate the cell-cycle and play a protective role in neurodegenerative disease. PAM-1 is a puromycin-sensitive aminopeptidase important for meiotic exit and polarity ... ...

    Abstract Puromycin-sensitive aminopeptidases are found across phyla and are known to regulate the cell-cycle and play a protective role in neurodegenerative disease. PAM-1 is a puromycin-sensitive aminopeptidase important for meiotic exit and polarity establishment in the one-cell Caenorhabditis elegans embryo. Despite conservation of this aminopeptidase, little is known about its targets during development. In order to identify novel interactors, we conducted a suppressor screen and isolated four suppressing mutations in three genes that partially rescued the maternal-effect lethality of pam-1 mutants. Suppressed strains show improved embryonic viability and polarization of the anterior-posterior axis. We identified a missense mutation in wee-1.3 in one of these suppressed strains. WEE-1.3 is an inhibitory kinase that regulates maturation promoting factor. Although the missense mutation suppressed polarity phenotypes in pam-1, it does so without restoring centrosome-cortical contact or altering the cortical actomyosin cytoskeleton. To see if PAM-1 and WEE-1.3 interact in other processes, we examined oocyte maturation. Although depletion of wee-1.3 causes sterility due to precocious oocyte maturation, this effect was lessened in pam-1 worms, suggesting that PAM-1 and WEE-1.3 interact in this process. Levels of WEE-1.3 were comparable between wild-type and pam-1 strains, suggesting that WEE-1.3 is not a direct target of the aminopeptidase. Thus, we have established an interaction between PAM-1 and WEE-1.3 in multiple developmental processes and have identified suppressors that are likely to further our understanding of the role of puromycin-sensitive aminopeptidases during development.
    MeSH term(s) Aminopeptidases/genetics ; Animals ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans Proteins/genetics ; Embryo, Nonmammalian ; Neurodegenerative Diseases ; Oocytes ; Protein Serine-Threonine Kinases ; Protein-Tyrosine Kinases
    Chemical Substances Caenorhabditis elegans Proteins ; wee-1.3 protein, C elegans (EC 2.7.1.-) ; Protein-Tyrosine Kinases (EC 2.7.10.1) ; Protein Serine-Threonine Kinases (EC 2.7.11.1) ; Aminopeptidases (EC 3.4.11.-) ; PepT tripeptidase (EC 3.4.11.14)
    Language English
    Publishing date 2021-03-08
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2629978-1
    ISSN 2160-1836 ; 2160-1836
    ISSN (online) 2160-1836
    ISSN 2160-1836
    DOI 10.1093/g3journal/jkab063
    Database MEDical Literature Analysis and Retrieval System OnLINE

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