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Article ; Online: β-catenin inhibitors in cancer therapeutics: intricacies and way forward.

Dev, Arundhathi / Vachher, Meenakshi / Prasad, Chandra Prakash

2023 Volume 14, Issue 1, Page(s) 2251696

Abstract: β-catenin is an evolutionary conserved, quintessential, multifaceted protein that plays vital roles in cellular homeostasis, embryonic development, organogenesis, stem cell maintenance, cell proliferation, migration, differentiation, apoptosis, and ... ...

Abstract	β-catenin is an evolutionary conserved, quintessential, multifaceted protein that plays vital roles in cellular homeostasis, embryonic development, organogenesis, stem cell maintenance, cell proliferation, migration, differentiation, apoptosis, and pathogenesis of various human diseases including cancer. β-catenin manifests both signaling and adhesive features. It acts as a pivotal player in intracellular signaling as a component of versatile WNT signaling cascade involved in embryonic development, homeostasis as well as in carcinogenesis. It is also involved in Ca
MeSH term(s)	Female ; Pregnancy ; Humans ; beta Catenin ; Neoplasms ; Carcinogenesis ; Cell Transformation, Neoplastic ; Signal Transduction
Chemical Substances	beta Catenin
Language	English
Publishing date	2023-09-01
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2737830-5
ISSN	2165-5987 ; 2165-5979
ISSN (online)	2165-5987
ISSN	2165-5979
DOI	10.1080/21655979.2023.2251696
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Article ; Online: Passage number of cancer cell lines: Importance, intricacies, and way-forward.

Prasad, Chandra Prakash / Tripathi, Satyendra Chandra / Kumar, Manish / Mohapatra, Purusottam

Biotechnology and bioengineering

2023 Volume 120, Issue 8, Page(s) 2049–2055

Abstract: Cancer cell lines play a crucial role as invaluable models in cancer research, facilitating the examination of cancer progression as well as the advancement of diagnostics and treatments. While they may not perfectly replicate the original tumor, they ... ...

Abstract	Cancer cell lines play a crucial role as invaluable models in cancer research, facilitating the examination of cancer progression as well as the advancement of diagnostics and treatments. While they may not perfectly replicate the original tumor, they generally exhibit similar characteristics. Low-passage cancer cell lines are generally preferred due to their closer resemblance to the original tumor, as long-term culturing can alter the genetic and molecular profiles of a cell line thereby highlighting the importance of monitoring the passage number (PN). Variations in proliferation, migration, gene expression, and drug sensitivity can be linked to PN differences. PN can also influence DNA methylation levels, metabolic profiles, and the expression of genes/or proteins in cancer cell lines. When conducting research on cancer cell lines, it is crucial for researchers to carefully select the appropriate PN to maintain consistency and reliability of results. Moreover, to ensure dependability and replicability, scientists ought to actively track the growth, migration, and gene/or protein profiles of cancer cell lines at specific PNs. This approach enables the identification of the most suitable range of PNs for experiments, guaranteeing consistent and precise results. Additionally, such efforts serve to minimize disparities and uphold the integrity of research. In this review, we have laid out recommendations for laboratories to overcome these PN discrepancies when working with cancer cell lines.
MeSH term(s)	Humans ; Reproducibility of Results ; Neoplasms/genetics ; Cell Line ; DNA Methylation/genetics ; Cell Line, Tumor
Language	English
Publishing date	2023-07-13
Publishing country	United States
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	280318-5
ISSN	1097-0290 ; 0006-3592
ISSN (online)	1097-0290
ISSN	0006-3592
DOI	10.1002/bit.28496
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Zs.B 960: Show issues

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Article ; Online: Essential role of aerobic glycolysis in epithelial-to-mesenchymal transition during carcinogenesis.

Prasad, Chandra Prakash / Gogia, Ajay / Batra, Atul

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico

2022 Volume 24, Issue 10, Page(s) 1844–1855

Abstract: Epithelial-to-mesenchymal transition (EMT) confers the most lethal characteristics to cancer cells i.e., metastasis and resistance to chemo-and-radio-therapy, and therefore exhibit an appealing target in the field of oncology. Research in the past decade ...

Abstract	Epithelial-to-mesenchymal transition (EMT) confers the most lethal characteristics to cancer cells i.e., metastasis and resistance to chemo-and-radio-therapy, and therefore exhibit an appealing target in the field of oncology. Research in the past decade has demonstrated the crucial role of aerobic glycolysis in EMT, which is generally credited as the glucose metabolism for the creation of biomass such as fatty acids, amino acids, and nucleotides thereby providing building blocks for limitless proliferation. In the present review, apart from discussing EMT's evident role in the metastatic process and cancer stemness, we also talked about the vital role of glycolytic enzymes viz. GLUTs, HKs, PGI, PFK-1, aldolase, enolase, PK, LDHA, etc. in the induction of the EMT process in cancerous cells.
MeSH term(s)	Carcinogenesis ; Epithelial-Mesenchymal Transition ; Glycolysis ; Humans ; Neoplasms ; Neoplastic Stem Cells
Language	English
Publishing date	2022-06-25
Publishing country	Italy
Document type	Journal Article ; Review
ZDB-ID	2397359-6
ISSN	1699-3055 ; 1699-048X
ISSN (online)	1699-3055
ISSN	1699-048X
DOI	10.1007/s12094-022-02851-6
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Zs.A 6644: Show issues

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Article ; Online: Publication Charges Associated with Quality Open Access (OA) Publishing and Its Impact on Low Middle Income Countries (LMICs), Time to Reframe Research Policies.

Singh, Mayank / Prasad, Chandra Prakash / Shankar, Abhishek

Asian Pacific journal of cancer prevention : APJCP

2021 Volume 22, Issue 9, Page(s) 2743–2747

Abstract: Dissemination of the scientific literature is as paramount as scientific studies. Scientific publishing has come a long way from localized distribution of few physical copies of journal to widespread and rapid distribution via internet in the 21st ... ...

Abstract	Dissemination of the scientific literature is as paramount as scientific studies. Scientific publishing has come a long way from localized distribution of few physical copies of journal to widespread and rapid distribution via internet in the 21st century. The evolution of open excess (OA) publishing which has rapidly evolved in last two decades has its heart at the right place with the ultimate goal being timely, and rapid distribution of published scientific work to a wider scientific community around the world and thus ultimately promoting scientific knowledge in global sense. However, quality OA publishing of cancer research involve an average publishing fee of around 1,500 USD which poses a challenge for Low middle income countries (LMICs), where per capita income is low. This has led to deterioration of science in LMICs in the form of publication in Cheap OA predatory journals for sake of securing academic promotions as well as authors ending up paying exorbitant publishing charges out of pocket to get their quality scientific work published. In countries like India and other LMICs, the funding agencies and institution have so far not addressed this problem. Here we assess the framework of open access publishing in LMICs like India and what are the steps which can be taken to facilitate open access publishing in LMICs.
MeSH term(s)	Biomedical Research ; Developing Countries ; Humans ; Open Access Publishing/economics ; Policy
Language	English
Publishing date	2021-09-01
Publishing country	Thailand
Document type	Journal Article ; Review
ZDB-ID	2218955-5
ISSN	2476-762X ; 1513-7368
ISSN (online)	2476-762X
ISSN	1513-7368
DOI	10.31557/APJCP.2021.22.9.2743
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Article: In vitro anticancer efficacy of a polyphenolic combination of Quercetin, Curcumin, and Berberine in triple negative breast cancer (TNBC) cells

Kashyap, Akanksha / Umar, Sheikh Mohammad / Dev J․R․, Arundhathi / Mendiratta, Mohini / Prasad, Chandra Prakash

Phytomedicine plus. 2022 May, v. 2, no. 2

2022

Abstract: Among all breast cancers, Triple negative breast cancer (TNBC) is the most aggressive subtype with an increased metastatic and relapse rate. Presently, the only therapeutic option available for TNBC patients is standard chemotherapy. Past research on ... ...

Abstract	Among all breast cancers, Triple negative breast cancer (TNBC) is the most aggressive subtype with an increased metastatic and relapse rate. Presently, the only therapeutic option available for TNBC patients is standard chemotherapy. Past research on natural compounds has demonstrated their chemopreventive as well as therapeutic effects in various cancers. Compounds like quercetin (QUE), curcumin (CUR), and berberine (BBR) have been shown to induce pro-apoptotic, anti-proliferative, anti-migratory, and anti-metastatic effects in various cancers. In the present study, we investigated the therapeutic potential of three natural compounds (QUE, CUR, and BBR) in the in vitro TNBC models. We also hypothesized that compared to their individual effects, combination treatment of all three polyphenols might enhance their in vitro efficacy in TNBCs. Using MDA-MB-468 and MDA-MB-231 as TNBC cell line models, we evaluated the effect of individual phytochemicals as well as their combined treatment (COMQᵁᴱ⁺Cᵁᴿ⁺ᴮᴮᴿ) on Epithelial mesenchymal transition (EMT) statuses, migration potential, cancer stem cells (CSCs), clonogenic/3D matrigel growth capability and drug resistance in TNBC cells. Our data demonstrated that the effect induced by the combination (COMQᵁᴱ⁺Cᵁᴿ⁺ᴮᴮᴿ) treatment on EMT proteins and cell migration were significant and synergistic, compared with individual treatments. Additionally, COMQᵁᴱ⁺Cᵁᴿ⁺ᴮᴮᴿ treatment significantly impaired the CD44⁺/CD24⁻population, clonogenic potential, and inherent cisplatin resistance in TNBC cells. For all our combination experiments, we have used 1/10thdose (to their respective IC₅₀concentrations), thereby showing strong efficacy of these phytochemicals in TNBC models. These findings demonstrate for the first time that combination QUE, CUR, and BBR has potential in the treatment of TNBCs, owing to its anti-proliferative, EMT inhibitory, and antagonistic cancer stemness functions.
Keywords	apoptosis ; berberine ; breast neoplasms ; breasts ; cell lines ; cell movement ; chemoprevention ; cisplatin ; curcumin ; drug resistance ; drug therapy ; epithelium ; metastasis ; polyphenols ; quercetin ; relapse
Language	English
Dates of publication	2022-05
Publishing place	Elsevier B.V.
Document type	Article
ISSN	2667-0313
DOI	10.1016/j.phyplu.2022.100265
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Combination therapy targeting the elevated interleukin-6 level reduces invasive migration of BRAF inhibitor-resistant melanoma cells.

Mohapatra, Purusottam / Prasad, Chandra Prakash / Andersson, Tommy

Molecular oncology

2019 Volume 13, Issue 2, Page(s) 480–494

Abstract: The identification of novel antimetastatic therapeutic targets is necessary for improved treatment of patients with acquired BRAF inhibitor-resistant (BRAFi-R) melanoma, in whom metastasis is a major concern. Our present study focused on the ... ...

Abstract	The identification of novel antimetastatic therapeutic targets is necessary for improved treatment of patients with acquired BRAF inhibitor-resistant (BRAFi-R) melanoma, in whom metastasis is a major concern. Our present study focused on the identification of such targets to explore novel antimetastatic therapeutic options for BRAFi-R melanoma patients. We confirmed the development of BRAFi resistance in our BRAFi-treated melanoma cell lines by demonstrating reduced sensitivity to BRAF inhibitors, increased ERK1/2 activity and increased WNT5A expression. Here, we demonstrated for the first time that high secretion of interleukin-6 (IL-6) was associated with increased invasive migration of BRAFi-R melanoma cells. This finding could be readily explained by the increased expression of WNT5A in BRAFi-R melanoma cells and the presence of an IL-6/WNT5A positive feedback loop in parental melanoma cells. Surprisingly, however, we found that the IL-6/WNT5A positive feedback loop present in parental melanoma cells was lost during the development of acquired BRAFi resistance, meaning that IL-6 and WNT5A signalling were independent events in BRAFi-R melanoma cells. Despite the absence of an IL-6/WNT5A loop, we found that both an IL-6 blocking antibody and the WNT5A antagonist Box5 alone impaired the elevated invasive migration of BRAFi-R melanoma cells, but combined use of the two was more effective. This impaired invasive migration of BRAFi-R melanoma cells correlated well with the reduction in Cdc42-GTPase activity and alterations of the actin cytoskeleton in these cells. In summary, our novel identification of IL-6 as a key independent promoter of the invasive migration of BRAFi-R melanoma cells stresses that a combination of a blocking IL-6 antibody and administration of the WNT5A antagonist Box5 might be an attractive antimetastatic approach for future treatment of BRAFi-R melanoma patients.
MeSH term(s)	Actins/metabolism ; Antibodies, Blocking/pharmacology ; Antineoplastic Combined Chemotherapy Protocols/pharmacology ; Antineoplastic Combined Chemotherapy Protocols/therapeutic use ; Cell Line, Tumor ; Cell Movement/drug effects ; Drug Resistance, Neoplasm/drug effects ; Humans ; Interleukin-6/metabolism ; Melanoma/drug therapy ; Melanoma/pathology ; Mutation/genetics ; Neoplasm Invasiveness ; Protein Kinase Inhibitors/pharmacology ; Protein Kinase Inhibitors/therapeutic use ; Proto-Oncogene Proteins B-raf/antagonists & inhibitors ; Proto-Oncogene Proteins B-raf/metabolism ; Signal Transduction/drug effects ; Wnt-5a Protein ; cdc42 GTP-Binding Protein/metabolism
Chemical Substances	Actins ; Antibodies, Blocking ; Interleukin-6 ; Protein Kinase Inhibitors ; WNT5A protein, human ; Wnt-5a Protein ; Proto-Oncogene Proteins B-raf (EC 2.7.11.1) ; cdc42 GTP-Binding Protein (EC 3.6.5.2)
Language	English
Publishing date	2019-01-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2415106-3
ISSN	1878-0261 ; 1574-7891
ISSN (online)	1878-0261
ISSN	1574-7891
DOI	10.1002/1878-0261.12433
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Dutta, Preyangsee / Saha, Dwaipayan / Earle, Mrudul / Prasad, Chandra Prakash / Singh, Mayank / Darswal, Mrinalini / Aggarwal, Vipul / Naik, Nitish / Yadav, Rakesh / Shankar, Abhishek / Chakraborty, Abhijit

Indian heart journal

2024 Volume 76, Issue 1, Page(s) 1–5

Abstract: Cardiovascular diseases (CVD) remain a major global health challenge, with an escalating impact on mortality despite advancements in managing conventional risk factors. This review investigates the intricate relationship between human papillomavirus (HPV) ...

Abstract	Cardiovascular diseases (CVD) remain a major global health challenge, with an escalating impact on mortality despite advancements in managing conventional risk factors. This review investigates the intricate relationship between human papillomavirus (HPV) and CVD, shedding light on a novel aspect of cardiovascular health. Despite significant progress in understanding and managing traditional CVD risk factors, a substantial proportion of CVD cases lack these conventional markers. Recent research has unveiled HPV, a prevalent sexually transmitted infection, as a potential unconventional risk factor for CVD. This review delves into the underlying mechanisms linking HPV to CVD pathogenesis. HPV's influence on vascular endothelium and induction of systemic inflammation are key contributors. Additionally, HPV disrupts host lipid metabolism, further exacerbating the development of atherosclerosis. The link between HPV and CAD is not merely correlative; it encompasses a complex interplay of virological, immunological, and metabolic factors. Understanding the connection between HPV and CVD holds transformative potential. Insights from this review not only underscore the significance of considering HPV as a crucial risk factor but also advocate for targeted HPV screening and vaccination strategies to mitigate CVD risks. This multidisciplinary exploration bridges the gap between infectious diseases and cardiovascular health, emphasizing the need for a comprehensive approach to combating the global burden of cardiovascular disease. Further research and clinical guidelines in this realm are essential to harness the full scope of preventive and therapeutic interventions, ultimately shaping a healthier cardiovascular landscape.
MeSH term(s)	Humans ; Papillomavirus Infections/complications ; Papillomavirus Infections/epidemiology ; Papillomavirus Infections/prevention & control ; Cardiovascular Diseases/epidemiology ; Cardiovascular Diseases/etiology ; Human Papillomavirus Viruses ; Risk Factors ; Atherosclerosis
Language	English
Publishing date	2024-02-20
Publishing country	India
Document type	Review ; Editorial
ZDB-ID	604366-5
ISSN	2213-3763 ; 0019-4832
ISSN (online)	2213-3763
ISSN	0019-4832
DOI	10.1016/j.ihj.2024.02.001
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Article: Quercetin Impairs HuR-Driven Progression and Migration of Triple Negative Breast Cancer (TNBC) Cells

Umar, Sheikh Mohammad / Patra, Sushmita / Kashyap, Akanksha / Dev J R, Arundhathi / Kumar, Lalit / Prasad, Chandra Prakash

Nutrition and cancer. 2022 Apr. 21, v. 74, no. 4

2022

Abstract: In the present study, we have explored the prognostic value of HuR gene as well as protein in breast cancers. Furthermore, we have also investigated the HuR therapeutic relevance in TNBCs, which is an aggressive breast cancer subtype. Using an online ... ...

Abstract	In the present study, we have explored the prognostic value of HuR gene as well as protein in breast cancers. Furthermore, we have also investigated the HuR therapeutic relevance in TNBCs, which is an aggressive breast cancer subtype. Using an online meta-analysis tool, we found that HuR protein overexpression positively correlates with reduced overall survival of TNBC patients (p = 0.028). Furthermore, we demonstrated that the TNBC breast cancer cell lines i.e., MDA-MB-231 and MDA-MB-468 are good model systems to study HuR protein, as they both exhibit a significant amount of cytoplasmic HuR (active form). Quercetin treatment significantly inhibited the cytoplasmic HuR in both TNBC cell lines. By using specific HuR siRNA, we established that quercetin-mediated inhibition of adhesion and migration of TNBC cells is dependent on HuR. Upon analyzing adhesion proteins i.e., β-catenin and CD44, we found that quercetin mediated effect on TNBC adhesion and migration was through the HuR-β-catenin axis and CD44, independently. Overall, the present results demonstrate that elevated HuR levels are associated with TNBC progression and relapse, and the ability of quercetin to inhibit cytoplasmic HuR protein provides a rationale for using it as an anticancer agent for the treatment of aggressive TNBCs. Supplemental data for this article is available online at at 10.1080/01635581.2021.1952628.
Keywords	adhesion ; antineoplastic agents ; breast neoplasms ; breasts ; genes ; meta-analysis ; neoplasm cells ; quercetin ; relapse ; therapeutics
Language	English
Dates of publication	2022-0421
Size	p. 1497-1510.
Publishing place	Taylor & Francis
Document type	Article
ZDB-ID	424433-3
ISSN	1532-7914 ; 0163-5581
ISSN (online)	1532-7914
ISSN	0163-5581
DOI	10.1080/01635581.2021.1952628
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Article: Role of Complementary and Alternative Medicine in Prevention and Treatment of COVID-19: An Overhyped Hope.

Shankar, Abhishek / Dubey, Anusha / Saini, Deepak / Prasad, Chandra Prakash

Chinese journal of integrative medicine

2020 Volume 26, Issue 8, Page(s) 565–567

MeSH term(s)	Betacoronavirus/physiology ; COVID-19 ; Complementary Therapies ; Coronavirus Infections/therapy ; Homeopathy ; Humans ; Medicine, Ayurvedic ; Medicine, Chinese Traditional ; Pandemics ; Pneumonia, Viral/therapy ; SARS-CoV-2
Keywords	covid19
Language	English
Publishing date	2020-08-05
Publishing country	China
Document type	Journal Article
ZDB-ID	2171254-2
ISSN	1993-0402 ; 1672-0415
ISSN (online)	1993-0402
ISSN	1672-0415
DOI	10.1007/s11655-020-2851-y
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Zs.A 5823: Show issues

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Article ; Online: Dihydrotanshinone-I modulates Epithelial Mesenchymal Transition (EMT) Thereby Impairing Migration and Clonogenicity of Triple Negative Breast Cancer Cells.

Kashyap, Akanksha / Umar, Sheikh Mohammad / Dev J R, Arundhathi / Prasad, Chandra Prakash

Asian Pacific journal of cancer prevention : APJCP

2021 Volume 22, Issue 7, Page(s) 2177–2184

Abstract: Background: Salvia miltiorrhiza Bunge (Danshen), has been used for its therapeutic value in Traditional Chinese Medicine (TCM), for almost a thousand years. Dihydrotanshinone-I (DHTS) is a lipophilic compound isolated from the plant Salvia miltiorrhiza ... ...

Abstract	Background: Salvia miltiorrhiza Bunge (Danshen), has been used for its therapeutic value in Traditional Chinese Medicine (TCM), for almost a thousand years. Dihydrotanshinone-I (DHTS) is a lipophilic compound isolated from the plant Salvia miltiorrhiza that has been shown to induce anti-proliferative and apoptotic effects on breast cancer cells. In the present study, we investigated the anti-migratory effect of DHTS on TNBC cell lines by studying the Epithelial Mesenchymal Transition (EMT) changes. Methods: IC50 values for DHTS in TNBC breast cancer cells were either discovered by literature search or by performing MTT assay. DHTS effect on EMT markers (viz. CD44, E-cadherin, Vimentin, N-cadherin, and active β-catenin) was studied using western blotting. Association between EMT and migration was further carried out in DHTS treated TNBC cells by wound healing assay. Cancer stemness and proliferation potential were further accessed using colony formation assay. Results: MTT assay revealed IC50 of MDA-MB-468 cells at 2 µM for 24 h. Subsequently, DHTS treatment in TNBC cell lines (MDA-MB-468 and MDA-MB-231) led to decrease in mesenchymal markers i.e. vimentin, N-cadherin and, active β-catenin. DHTS treated MDA-MB-468 cells showed a decrease in adhesion protein CD44 and an increase in epithelial protein E-cadherin. Additionally, a decrease in EMT potential was positively associated with the inhibition of migration and clonogenic potential in DHTS treated TNBC cells. Conclusion: In this study, we have demonstrated for the first time that DHTS has the potential to inhibit the migration and clonogenicity of highly aggressive TNBC cells by obstructing Epithelial to Mesenchymal Transition.
MeSH term(s)	Biomarkers, Tumor/metabolism ; Cell Line, Tumor ; Cell Movement/drug effects ; Cell Proliferation/drug effects ; Epithelial-Mesenchymal Transition/drug effects ; Furans/pharmacology ; Humans ; Phenanthrenes/pharmacology ; Plant Extracts/pharmacology ; Quinones/pharmacology ; Triple Negative Breast Neoplasms/drug therapy ; Triple Negative Breast Neoplasms/metabolism
Chemical Substances	Biomarkers, Tumor ; Furans ; Phenanthrenes ; Plant Extracts ; Quinones ; dihydrotanshinone I (562G9360V6)
Language	English
Publishing date	2021-07-01
Publishing country	Thailand
Document type	Journal Article
ZDB-ID	2218955-5
ISSN	2476-762X ; 1513-7368
ISSN (online)	2476-762X
ISSN	1513-7368
DOI	10.31557/APJCP.2021.22.7.2177
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