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  1. AU="Prasad Sarkale"
  2. AU="Manfredonia, Laura"
  3. AU="Linssen, L"
  4. AU="Davide, Borroni"
  5. AU="Ingrid M. Wentzensen"
  6. AU="A.Parida, "
  7. AU="Zhu, D H"
  8. AU=Ulloa Luis
  9. AU="Böhme, Elisa"
  10. AU=Trko?lu Oya
  11. AU="Levine, Zoe"
  12. AU="Banaszkiewicz, Paul A."
  13. AU="Datrier, Laurence E. H."
  14. AU=Fala Loretta
  15. AU="McGuckin, M M"
  16. AU="Winlaw, David S"
  17. AU="Gökmen, M Refik"
  18. AU="Islam, Tousif"
  19. AU="Szczepanczyk, Marek J"
  20. AU="Boregowda, Siddaraju"
  21. AU="Lomidzew, D."

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  1. Artikel ; Online: Development of single step RT-PCR for detection of Kyasanur forest disease virus from clinical samples

    Gouri Chaubal / Prasad Sarkale / Pravin Kore / Pragya Yadav

    Heliyon, Vol 4, Iss

    2018  Band 2

    Abstract: Background: Kyasanur Forest Disease (KFD), a tick borne flavivirus, which was earlier endemic to Karnataka state, India, has been confirmed and detected from neighboring states of Tamil Nadu, Maharashtra, Goa and Kerala states in India. Increased human ... ...

    Abstract Background: Kyasanur Forest Disease (KFD), a tick borne flavivirus, which was earlier endemic to Karnataka state, India, has been confirmed and detected from neighboring states of Tamil Nadu, Maharashtra, Goa and Kerala states in India. Increased human and vector surveillance therefore becomes essential for the identification of KFD affected regions and control of further spread of the disease. Currently, available KFD detection assays include realtime RT-PCR and nested RT-PCR assays. Here we describe the development of a sensitive single step RT-PCR assay for the detection of KFD viral RNA. This can be easily used in any BSL-2 laboratory for screening of KFD suspected cases or for differential diagnosis of viral hemorrhagic fever panel. Method: Three primer sets were designed and checked for sensitivity using known dilutions of KFD viral RNA (Ranging from 106 copies to 10 copies). The primer set (2) was found to be most sensitive was selected and tested for specificity for Kyasanur forest disease virus (KFDV) by testing against zika, dengue, chikungunya, crimean congo hemorrhagic fever (CCHF), yellow fever, japanese encephalitis (JE) and west nile viruses. A total of 104 samples (human, monkey and tick positive and negative samples) were tested using this assay. Result: No false positive or false negative results were seen for human, monkey or tick samples. The assay was specific for KFD and could detect upto 100 copies of KFD viral RNA. Discussion and conclusion: The previously published sensitive real time RT-PCR assay requires higher cost in terms of reagents and machine setup and technical expertise has been the primary reason for development of this assay. A single step RT-PCR is relatively easy to perform and more cost effective than real time RT-PCR in smaller setups in the absence of Biosafety Level-3 facility. This study reports the development and optimization of single step RT-PCR assay which is more sensitive and less time-consuming than nested RT-PCR and cost effective for rapid diagnosis of KFD ...
    Schlagwörter Infectious disease ; Molecular biology ; Virology ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
    Thema/Rubrik (Code) 630
    Sprache Englisch
    Erscheinungsdatum 2018-02-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: Isolation of SARS-CoV-2 B.1.1.28.2 (P2) variant and pathogenicity comparison with D614G variant in hamster model

    Pragya Yadav / Sreelekshmy Mohandas / Prasad Sarkale / Dimpal Nyayanit / Anita Shete / Rima Sahay / Varsha Potdar / Shrikant Baradkar / Nivedita Gupta / Gajanan Sapkal / Priya Abraham / Samiran Panda / Balram Bhargava

    Journal of Infection and Public Health, Vol 15, Iss 2, Pp 164-

    2022  Band 171

    Abstract: Background: Considering the potential threat from emerging Severe Acute Respiratory Syndrome-Corona Virus-2 (SARS-CoV-2) variants and the rising COVID-19 cases, SARS-CoV-2 genomic surveillance is ongoing in India. We report herewith the isolation of the ... ...

    Abstract Background: Considering the potential threat from emerging Severe Acute Respiratory Syndrome-Corona Virus-2 (SARS-CoV-2) variants and the rising COVID-19 cases, SARS-CoV-2 genomic surveillance is ongoing in India. We report herewith the isolation of the P.2 variant (B.1.1.28.2) from international travelers and further its pathogenicity evaluation and comparison with D614G variant (B.1) in hamster model. Methods: Virus isolation was performed in Vero CCL81 cells and genomic characterization by next generation sequencing. The pathogenicity and host immune response of the isolate was assessed in Syrian hamster model and compared with B.1 variant. Results: B.1.1.28.2 variant was isolated from nasal/throat swabs of international travelers returned to India from United Kingdom and Brazil. The B.1.1.28.2 variant induced body weight loss, viral replication in the respiratory tract and caused severe lung pathology in infected Syrian hamster model in comparison, with B.1 variant infected hamsters. The sera from B.1.1.28.2 infected hamsters efficiently neutralized the D614G variant virus whereas 6-fold reduction in the neutralization was seen in case of D614G variant infected hamsters’ sera with the B.1.1.28.2 variant. Conclusions: B.1.1.28.2 lineage variant could be successfully isolated and characterization could be performed. Pathogenicity of the isolate was demonstrated in Syrian hamster model and the findings of neutralization reduction is of great concern and point towards the need for screening the vaccines for efficacy.
    Schlagwörter SARS-CoV-2 ; B.1.1.28.2 ; P2 variant ; India ; Pathogenicity ; Infectious and parasitic diseases ; RC109-216 ; Public aspects of medicine ; RA1-1270
    Thema/Rubrik (Code) 572
    Sprache Englisch
    Erscheinungsdatum 2022-02-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel ; Online: Characterization of a strain of quaranfil virus isolated from soft ticks in India. Is quaranfil virus an unrecognized cause of disease in human and animals?”

    Devendra T. Mourya / Pragya D. Yadav / Dimpal A. Nyayanit / Triparna D. Majumdar / Shilpi Jain / Prasad Sarkale / Anita Shete

    Heliyon, Vol 5, Iss 3, Pp e01368- (2019)

    2019  

    Abstract: The soft ticks collected during a field survey in Karnataka state, India, in 1983, yielded a novel virus isolate, which caused mortality in an infant mouse upon inoculation. Attempts at characterizing the virus using the conventional methods were ... ...

    Abstract The soft ticks collected during a field survey in Karnataka state, India, in 1983, yielded a novel virus isolate, which caused mortality in an infant mouse upon inoculation. Attempts at characterizing the virus using the conventional methods were unsuccessful, which prompted us to study it by Next-Generation Sequencing (NGS). This virus isolate was obtained from the viral repository of National Institute of Virology, and an initial virus stock was prepared as a mouse brain homogenate. The virus stock showed cytopathic effects in different cell-lines and was used in NGS. Based on the complete genome sequence, obtained using de novo and reference mapping approach, the virus isolate was identified as a Quaranfil virus (QRFV) belonging to the family Orthomyxoviridae, genus Quaranjavirus. The genome size of the virus is 11,427 nucleotides which consist of 6 segments encoding six proteins. Homology analysis suggested this isolate as similar to QRFV of Afghanistan. In silico analysis showed the HA protein secondary structure to be a class III penetrance similar to Thogotovirus. QRFV was first isolated in 1953 from ticks [Cairo, Egypt] and subsequently reported from other geographical areas. This is the first report describing the presence of QRFV from India. This discovery emphasizes the need for investigating mild febrile illness cases with influenza-like symptoms, particularly in the area of high risk for tick bites.
    Schlagwörter Bioinformatics ; Virology ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
    Thema/Rubrik (Code) 616
    Sprache Englisch
    Erscheinungsdatum 2019-03-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  4. Artikel ; Online: Isolation and characterization of SARS-CoV-2 Beta variant from UAE travelers

    Pragya D. Yadav / Prasad Sarkale / Alpana Razdan / Nivedita Gupta / Dimpal A. Nyayanit / Rima R. Sahay / Varsha Potdar / Deepak Y. Patil / Shreekant Baradkar / Abhinendra Kumar / Neeraj Aggarwal / Anita M. Shete / Harmanmeet Kaur / Sreelekshmy Mohandas

    Journal of Infection and Public Health, Vol 15, Iss 2, Pp 182-

    2022  Band 186

    Abstract: Background: The emergence of SARS-CoV-2 variants in places where the virus is uncontained poses a global threat from the perspective of public health and vaccine efficacy. Travel has been important factor for the easy spread of SARS-CoV-2 variants ... ...

    Abstract Background: The emergence of SARS-CoV-2 variants in places where the virus is uncontained poses a global threat from the perspective of public health and vaccine efficacy. Travel has been important factor for the easy spread of SARS-CoV-2 variants worldwide. India has also observed the importation of SARS-CoV-2 variants through international travelers. Methods: In this study, we have collected the oropharyngeal and nasopharyngeal swab specimens from 58 individuals with travel history from United Arab Emirates (UAE), East, West and South Africa, Qatar, Ukraine and Saudi Arabia arrived in India during February–March 2021. The clinical specimens were initially screened for SARS-CoV-2 using Real time RT-PCR. All the specimens were inoculated on to Vero CCL-81 cells for virus isolation. The viral isolates were further sequenced using Next-Generation Sequencing. Results: All 58 cases were tested positive for SARS-CoV-2 using Real time RT-PCR. Four specimens showed progressive infectivity with fusion of the infected cells with neighboring cells leading to large mass of cells. Replication competent virus was confirmed from culture supernatant of the passage 2 using Real time RT-PCR. Two plaque purified SARS-CoV-2 isolates demonstrated high viral RNA load of 3.8–7.5 × 1011 and 1.1–1.6 × 1011 at passage 4 and 5 respectively. Nucleotide variations along with amino acid changes were also observed among these two isolates at passage 2–5. All four cases were male with no symptoms and co-morbidity. The sequence analysis has shown two different clusters, first cluster with nucleotide deletions in the ORF1ab and the spike, while second cluster with deletions in spike region. The viral isolates demonstrated 99.88–99.96% nucleotide identity with the representative sequences of Beta variant (B.1.351). Conclusion: These findings suggest easier transmission of SARS-CoV-2 variants with human mobility through international travel. The isolated Beta variant would be useful to determine the protective efficacy of the currently available ...
    Schlagwörter Variant of concern ; SARS-CoV-2 ; Beta variant ; Traveler ; United Arab Emirates ; India ; Infectious and parasitic diseases ; RC109-216 ; Public aspects of medicine ; RA1-1270
    Sprache Englisch
    Erscheinungsdatum 2022-02-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  5. Artikel ; Online: Possible Role of Accessory Proteins in the Viral Replication for the 20I/501Y.V1 (B.1.1.7) SARS CoV-2 Variant

    Dimpal A. Nyayanit / Prasad Sarkale / Anita Shete-Aich / Abhinendra Kumar / Savita Patil / Triparna Majumdar / Shrikant Baradkar / Pranita Gawande / Sreelekshmy Mohandas / Pragya D Yadav

    Pathogens, Vol 10, Iss 1586, p

    2021  Band 1586

    Abstract: The emergence of new severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) has been a global concern. The B.1.1.7 variant of SARS CoV-2 is reported to cause higher transmission. The study investigates the replication cycle and transcriptional ... ...

    Abstract The emergence of new severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) has been a global concern. The B.1.1.7 variant of SARS CoV-2 is reported to cause higher transmission. The study investigates the replication cycle and transcriptional pattern of the B.1.1.7 to hypothesis the possible role of different genes in viral replication. It was observed that the B.1.1.7 variant required a longer maturation time. The transcriptional response demonstrated higher expression of ORF6 and ORF8 compared to nucleocapsid transcript till the eclipse period which might influence higher viral replication. The number of infectious viruses titer is higher in the B.1.1.7, despite a lesser copy number than B.1, indicating higher transmissibility. The experimental evidence published linked ORF6 and ORF8 to play important role in replication and we also observed their higher expression. This leads us to hypothesis the possible role of ORF6 and ORF8 in B.1.1.7 higher replication which causes higher transmission.
    Schlagwörter 20I/501Y.V1 ; SARS CoV-2 ; replication ; RPKM ; TCID 50 ; next-generation sequencing ; Medicine ; R
    Thema/Rubrik (Code) 570
    Sprache Englisch
    Erscheinungsdatum 2021-12-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  6. Artikel ; Online: Transcriptome & viral growth analysis of SARS-CoV-2-infected Vero CCL-81 cells

    Dimpal A Nyayanit / Prasad Sarkale / Shreekant Baradkar / Savita Patil / Pragya D Yadav / Anita Shete-Aich / Kaumudi Kalele / Pranita Gawande / Triparna Majumdar / Rajlaxmi Jain / Gajanan Sapkal

    Indian Journal of Medical Research, Vol 152, Iss 1, Pp 70-

    2020  Band 76

    Abstract: Background & objectives: The genome of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), belonging to the family Coronaviridae, encodes for structural, non-structural, and accessory proteins, which are required for replication of the virus. ... ...

    Abstract Background & objectives: The genome of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), belonging to the family Coronaviridae, encodes for structural, non-structural, and accessory proteins, which are required for replication of the virus. These proteins are encoded by different genes present on the SARS-CoV-2 genome. The expression pattern of these genes in the host cells needs to be assessed. This study was undertaken to understand the transcription pattern of the SARS-CoV-2 genes in the Vero CCL-81 cells during the course of infection. Methods: Vero CCL-81 cells were infected with the SARS-CoV-2 virus inoculum having a 0.1 multiplicity of infection. The supernatants and cell pellets were harvested after centrifugation at different time points, post-infection. The 50% tissue culture infective dose (TCID50)and cycle threshold (Ct) values of the E and the RdRp-2 genes were calculated. Next-generation sequencing of the harvested sample was carried out to observe the expression pattern of the virus by mapping to the SARS-CoV-2 Wuhan HU-1 reference sequence. The expressions were in terms of the reads per kilobase million (RPKM) values. Results: In the inital six hours post-infection, the copy numbers of E and RdRp-2 genes were approximately constant, which raised 10 log-fold and continued to increase till the 12 h post-infection (hpi). The TCID50 was observed in the supernatant after 7 hpi, indicating the release of the viral progeny. ORF8 and ORF7a, along with the nucleocapsid transcript, were found to express at higher levels. Interpretation & conclusions: This study was a step towards understanding the growth kinetics of the SARS-CoV-2 replication cycle. The findings indicated that ORF8 and ORF7b gene transcripts were expressed in higher amounts indicating their essential role in viral replication. Future studies need to be conducted to explore their role in the SARS-CoV-2 replication.
    Schlagwörter india - next-generation sequencing - rt-pcr - replication cycle - sars-cov-2 - transcriptome ; Medicine ; R ; covid19
    Thema/Rubrik (Code) 570
    Sprache Englisch
    Erscheinungsdatum 2020-01-01T00:00:00Z
    Verlag Wolters Kluwer Medknow Publications
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  7. Artikel ; Online: Neutralizing antibody responses to SARS-CoV-2 in COVID-19 patients

    Gururaj Rao Deshpande / Gajanan N Sapkal / Bipin N Tilekar / Pragya D Yadav / Yogesh Gurav / Shivshankar Gaikwad / Himanshu Kaushal / Ketki S Deshpande / Ojas Kaduskar / Prasad Sarkale / Srikant Baradkar / Annasaheb Suryawanshi / Rajen Lakra / A P Sugunan / Anukumar Balakrishnan / Priya Abraham / Pavan Salve

    Indian Journal of Medical Research, Vol 152, Iss 1, Pp 82-

    2020  Band 87

    Abstract: Background & objectives: The global pandemic caused by SARS-CoV-2 virus has challenged public health system worldwide due to the unavailability of approved preventive and therapeutic options. Identification of neutralizing antibodies (NAb) and ... ...

    Abstract Background & objectives: The global pandemic caused by SARS-CoV-2 virus has challenged public health system worldwide due to the unavailability of approved preventive and therapeutic options. Identification of neutralizing antibodies (NAb) and understanding their role is important. However, the data on kinetics of NAb response among COVID-19 patients are unclear. To understand the NAb response in COVID-19 patients, we compared the findings of microneutralization test (MNT) and plaque reduction neutralization test (PRNT) for the SARS-CoV-2. Further, the kinetics of NAb response among COVID-19 patients was assessed. Methods: A total of 343 blood samples (89 positive, 58 negative for SARS-CoV-2 and 17 cross-reactive and 179 serum from healthy individuals) were collected and tested by MNT and PRNT. SARS-CoV-2 virus was prepared by propagating the virus in Vero CCL-81 cells. The intra-class correlation was calculated to assess the correlation between MNT and PRNT. The neutralizing endpoint as the reduction in the number of plaque count by 90 per cent (PRNT90) was also calculated. Results: The analysis of MNT and PRNT quantitative results indicated that the intra-class correlation was 0.520. Of the 89 confirmed COVID-19 patients, 64 (71.9%) showed NAb response. Interpretation & conclusions: The results of MNT and PRNT were specific with no cross-reactivity. In the early stages of infection, the NAb response was observed with variable antibody kinetics. The neutralization assays can be used for titration of NAb in recovered/vaccinated or infected COVID-19 patients.
    Schlagwörter antibody kinetics - covid-19 - mnt - neutralizing antibody response - prnt - sars-cov-2 ; Medicine ; R ; covid19
    Thema/Rubrik (Code) 616
    Sprache Englisch
    Erscheinungsdatum 2020-01-01T00:00:00Z
    Verlag Wolters Kluwer Medknow Publications
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  8. Artikel ; Online: Immunogenicity and protective efficacy of BBV152, whole virion inactivated SARS- CoV-2 vaccine candidates in the Syrian hamster model

    Sreelekshmy Mohandas / Pragya D. Yadav / Anita Shete-Aich / Priya Abraham / Krishna Mohan Vadrevu / Gajanan Sapkal / Chandrashekhar Mote / Dimpal Nyayanit / Nivedita Gupta / Vellimedu Kannappa Srinivas / Manoj Kadam / Abhimanyu Kumar / Triparna Majumdar / Rajlaxmi Jain / Gururaj Deshpande / Savita Patil / Prasad Sarkale / Deepak Patil / Raches Ella /
    Sai D. Prasad / Sharda Sharma / Krishna M. Ella / Samiran Panda / Balram Bhargava

    iScience, Vol 24, Iss 2, Pp 102054- (2021)

    2021  

    Abstract: Summary: The availability of a safe and effective vaccine would be the eventual measure to deal with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) threat. Here, we have assessed the immunogenicity and protective efficacy of inactivated ... ...

    Abstract Summary: The availability of a safe and effective vaccine would be the eventual measure to deal with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) threat. Here, we have assessed the immunogenicity and protective efficacy of inactivated SARS-CoV-2 vaccine candidates BBV152A, BBV152B, and BBV152C in Syrian hamsters. Three dose vaccination regimes with vaccine candidates induced significant titers of SARS-CoV-2-specific IgG and neutralizing antibodies. BBV152A and BBV152B vaccine candidates remarkably generated a quick and robust immune response. Post-SARS-CoV-2 infection, vaccinated hamsters did not show any histopathological changes in the lungs. The protection of the hamster was evident by the rapid clearance of the virus from lower respiratory tract, reduced virus load in upper respiratory tract, absence of lung pathology, and robust humoral immune response. These findings confirm the immunogenic potential of the vaccine candidates and further protection of hamsters challenged with SARS-CoV-2. Of the three candidates, BBV152A showed the better response.
    Schlagwörter Biological Sciences ; Immunity ; Immunology ; Viral Microbiology ; Virology ; Science ; Q
    Thema/Rubrik (Code) 630
    Sprache Englisch
    Erscheinungsdatum 2021-02-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  9. Artikel ; Online: Clinico-epidemiological investigation on Varicella Zoster Virus indicates multiple clade circulation in Maharashtra state, India

    Rima R. Sahay / Pragya D. Yadav / Triparna Majumdar / Swapnil Patil / Prasad Sarkale / Anita M. Shete / Gouri Chaubal / Vinay R. Dange / Savita Patil / Dimpal A. Nyayanit / Jayanthi Shastri / Devendra T. Mourya

    Heliyon, Vol 4, Iss 8, Pp e00757- (2018)

    2018  

    Abstract: Background: Varicella Zoster Virus (VZV) is consistently in circulation and shows an increase in disease burden during the spring season. Due to a wide range of clinical presentation from a vesicular rash to bleeding or neurological complications, it ... ...

    Abstract Background: Varicella Zoster Virus (VZV) is consistently in circulation and shows an increase in disease burden during the spring season. Due to a wide range of clinical presentation from a vesicular rash to bleeding or neurological complications, it makes the clinical diagnosis difficult. The present study aims to understand whether the same strain of virus is responsible for the increase in the seasonal outbreaks occurring in different parts of the country with reference to the samples from Maharashtra, Rajasthan and Gujarat states of India. Materials and methods: This study reports the clinico-epidemiological and laboratory findings of suspected Varicella cases. To understand the circulating clade few representative real-time Polymerase Chain Reaction (PCR) positive were analyzed by conventional PCR and partial Open Reading Frame (ORF) 22, partial ORF 38 and partial ORF 54 were sequenced to identify single nucleotide polymorphisms responsible for clade determination. Further partial glycoprotein B gene was sequenced, and a phylogenetic tree was generated. Results: A total of 50 cases from Maharashtra (Mumbai district) and referred clinical samples of Rajasthan (Barmer district; n = 12) and Gujarat States (Gandhi Nagar, Surat districts; n = 17) were tested for the presence of VZV. Vesicular rash with fever was a common clinical presentation with 82% cases having contact history with VZV positive cases, suggesting higher secondary attack rate. The vesicular fluid of all 50 cases from Mumbai revealed the presence of VZV by real-time PCR. Urine, serum and throat swab samples showed positivity by real-time PCR. Healthcare provider's samples from Rajasthan showed 36.4% [4/11] positivity. Clinical samples from Gujarat had positivity of 41.2% [7/17]. Conclusions: This study analyses the clade based circulation of VZV in three states in India and suggests different clades circulating in Maharashtra state. Health education amongst the general population is suggested to reduce the secondary cases by early diagnosis, ...
    Schlagwörter Epidemiology ; Infectious disease ; Public health ; virology ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
    Thema/Rubrik (Code) 630
    Sprache Englisch
    Erscheinungsdatum 2018-08-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  10. Artikel ; Online: Circulation of Nipah virus in Pteropus giganteus bats in northeast region of India, 2015

    Pragya Yadav / Anakkathil Sudeep / Mangesh Gokhale / Shailesh Pawar / Anita Shete / Deepak Patil / Vimal Kumar / Rajen Lakra / Prasad Sarkale / Stuart Nichol / Devendra Mourya

    Indian Journal of Medical Research, Vol 147, Iss 3, Pp 318-

    2018  Band 320

    Schlagwörter Medicine ; R
    Sprache Englisch
    Erscheinungsdatum 2018-01-01T00:00:00Z
    Verlag Wolters Kluwer Medknow Publications
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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