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Article: The effect of mesenchymal stem cell-conditioned medium gel on burn wound healing in rat.

Laksmitawati, Dian Ratih / Noor, Siti Umrah / Sumiyati, Yati / Hartanto, Adrian / Widowati, Wahyu / Pratami, Diah Kartika

Veterinary world

2022 Volume 15, Issue 4, Page(s) 841–847

Abstract: Background and aim: Stem cells are cells that can proliferate to form a new tissue, leading to its use in regenerative therapy. Stem cells will secrete biological factors, such as growth factors, cytokines, and other proteins to their surroundings and ... ...

Abstract	Background and aim: Stem cells are cells that can proliferate to form a new tissue, leading to its use in regenerative therapy. Stem cells will secrete biological factors, such as growth factors, cytokines, and other proteins to their surroundings and culture medium/conditioned medium (CM), altering tissue physiology. These factors can help wound healing, but their effect on third-degree burns is poorly understood. This research aimed to study the activity of mesenchymal stem cell-conditioned medium gel in healing and repairing third-degree burns on rats skin. Materials and methods: Twenty-four Sprague-Dawley rats with burn wounds on the dorsal area were divided into four groups; the first group was treated with CM gel, with a concentration equivalent to 0.05% protein, the second group was treated with a placebo gel, the third group with silver sulfadiazine (SSD) cream (SSD-Burnazin contain 10 mg/g SSD), and the fourth group was not given any treatment, for 21 days, and on the final day, the rats were sacrificed, and the skins were taken. All topical treatments completely cover the wound area. Results: Wound healing process indicators observed include wound diameter, scabs' formation, blister formation, and hair growth every day. The skins taken were processed with hematoxylin-eosin and Masson's trichrome staining. The indicators studied include neutrophil infiltration, mononuclear cell infiltration, neovascularization, collagen area, and re-epithelization ratio. Conclusion: CM shows better wound healing than other groups and faster hair growth.
Language	English
Publishing date	2022-04-07
Publishing country	India
Document type	Journal Article
ZDB-ID	2456277-4
ISSN	2231-0916 ; 0972-8988
ISSN (online)	2231-0916
ISSN	0972-8988
DOI	10.14202/vetworld.2022.841-847
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: In-vitro antioxidant, in-vivo anti-inflammatory, and acute toxicity study of Indonesian propolis capsule from

Farida, Siti / Pratami, Diah Kartika / Sahlan, Muhamad / Laksmitawati, Dian Ratih / Rohmatin, Etin / Situmorang, Herbert

Saudi journal of biological sciences

2021 Volume 29, Issue 4, Page(s) 2489–2500

Abstract: Propolis is widely used as traditional medicine since ancient times. It was necessary to conduct the pre-clinical study because of its relevant curative properties. This study aimed to investigate in-vitro antioxidant, standardize quality parameters, ... ...

Abstract	Propolis is widely used as traditional medicine since ancient times. It was necessary to conduct the pre-clinical study because of its relevant curative properties. This study aimed to investigate in-vitro antioxidant, standardize quality parameters, study acute toxicity, and determine in-vivo anti-inflammatory. Three spectrophotometric methods were used to determine antioxidant activity. The standardization includes physical, chemical, and microbiological evaluation. Furthermore, an acute toxicity test was conducted using 20 female Sprague Dawley (SD) strain rats divided into 4 groups with different dose of propolis. The in vivo anti-inflammatory test was carried out using the carrageenan induction method on rats' soles. A total of 36 female SD rats were classified into 6 groups as follows, Group normal, negative control, diclofenac sodium, and three propolis groups (72; 144; and 288 mg/kg BW). The results demonstrated the IC
Language	English
Publishing date	2021-12-16
Publishing country	Saudi Arabia
Document type	Journal Article
ZDB-ID	2515206-3
ISSN	2213-7106 ; 1319-562X
ISSN (online)	2213-7106
ISSN	1319-562X
DOI	10.1016/j.sjbs.2021.12.034
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Metabolome analysis and chemical profiling of Indonesian royal jellies as the raw material for cosmetic and bio-supplement products.

Sari, Eka / Mahira, Kaysa Faradis / Patel, Dhavalkumar Narendrabhai / Chua, Lee Suan / Pratami, Diah Kartika / Sahlan, Muhamad

Heliyon

2021 Volume 7, Issue 5, Page(s) e06912

Abstract: Royal jellies (RJs) possess moisturizing, emulsifying, and stabilizing properties, and several pharmacological activities have also been found to be present, which make them an ideal component for cosmetic and skin care products. However, despite the ... ...

Abstract	Royal jellies (RJs) possess moisturizing, emulsifying, and stabilizing properties, and several pharmacological activities have also been found to be present, which make them an ideal component for cosmetic and skin care products. However, despite the abundant efficacies, there is a lack of studies that explore the chemical composition of RJ using metabolome analysis. Furthermore, an evaluation of the chemical composition of Indonesian RJs collected from different regions has yet to be carried out. Therefore, the main objective of this study was to identify any differences in the chemical composition of such RJs. Chemical profiling was also carried out to enable more targeted utilization based on the actual compositions. Chemical profiling is also important given the rich Indonesian biodiversity and the high dependence of the RJ compositions on the botanical source. In this research, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used as part of an untargeted metabolomics approach. From the chemical profiling, >30 compounds were identified across four RJ samples. The major constituents of the samples were found to be oligosaccharides, fatty acids, and adenosine monophosphate derivatives. Meanwhile, sucrose and planteose were found to be highest in the samples from Banjarnegara and Kediri, whereas dimethyloctanoic acid was found to be unique to the sample from Banjarnegara. It was also discovered that the RJs from Demak and Tuban contained more organic fatty acids and oligosaccharides than the other samples. Although the sample from Demak demonstrated good potential for use in the cosmetic, skin care, and bio-supplement industries, the higher abundance of fatty acids and oligosaccharides in the sample from Tuban indicated that it is perhaps the most suitable RJ for use in this field.
Language	English
Publishing date	2021-05-03
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2021.e06912
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Metabolome analysis and chemical profiling of Indonesian royal jellies as the raw material for cosmetic and bio-supplement products

Sari, Eka / Mahira, Kaysa Faradis / Patel, Dhavalkumar Narendrabhai / Chua, Lee Suan / Pratami, Diah Kartika / Sahlan, Muhamad

Heliyon. 2021 May, v. 7, no. 5

2021

Abstract	Royal jellies (RJs) possess moisturizing, emulsifying, and stabilizing properties, and several pharmacological activities have also been found to be present, which make them an ideal component for cosmetic and skin care products. However, despite the abundant efficacies, there is a lack of studies that explore the chemical composition of RJ using metabolome analysis. Furthermore, an evaluation of the chemical composition of Indonesian RJs collected from different regions has yet to be carried out. Therefore, the main objective of this study was to identify any differences in the chemical composition of such RJs. Chemical profiling was also carried out to enable more targeted utilization based on the actual compositions. Chemical profiling is also important given the rich Indonesian biodiversity and the high dependence of the RJ compositions on the botanical source. In this research, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used as part of an untargeted metabolomics approach. From the chemical profiling, >30 compounds were identified across four RJ samples. The major constituents of the samples were found to be oligosaccharides, fatty acids, and adenosine monophosphate derivatives. Meanwhile, sucrose and planteose were found to be highest in the samples from Banjarnegara and Kediri, whereas dimethyloctanoic acid was found to be unique to the sample from Banjarnegara. It was also discovered that the RJs from Demak and Tuban contained more organic fatty acids and oligosaccharides than the other samples. Although the sample from Demak demonstrated good potential for use in the cosmetic, skin care, and bio-supplement industries, the higher abundance of fatty acids and oligosaccharides in the sample from Tuban indicated that it is perhaps the most suitable RJ for use in this field.
Keywords	adenosine monophosphate ; biodiversity ; chemical composition ; mass spectrometry ; metabolomics ; raw materials ; sucrose ; ultra-performance liquid chromatography
Language	English
Dates of publication	2021-05
Publishing place	Elsevier Ltd
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2021.e06912
Database	NAL-Catalogue (AGRICOLA)

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Article: Surface plasmon resonance analysis for detecting non-structural protein 1 of dengue virus in Indonesia.

Widoretno / Sjahrurachman, Agus / Dewi, Beti Ernawati / Lischer, Kenny / Pratami, Diah Kartika / Flamandita, Darin / Sahlan, Muhamad

Saudi journal of biological sciences

2020 Volume 27, Issue 8, Page(s) 1931–1937

Abstract: Dengue is an acute febrile disease caused by dengue virus (DENV) that is transmitted ... ...

Abstract	Dengue is an acute febrile disease caused by dengue virus (DENV) that is transmitted by
Language	English
Publishing date	2020-06-17
Publishing country	Saudi Arabia
Document type	Journal Article
ZDB-ID	2515206-3
ISSN	2213-7106 ; 1319-562X
ISSN (online)	2213-7106
ISSN	1319-562X
DOI	10.1016/j.sjbs.2020.06.018
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: The Effects of stingless bee (

Sahlan, Muhamad / Rahmawati, Oktavia / Pratami, Diah Kartika / Raffiudin, Rika / Mukti, Rino Rakhmata / Hermasyah, Heri

Saudi journal of biological sciences

2019 Volume 27, Issue 8, Page(s) 2025–2030

Abstract: Diabetes mellitus (DM) is a metabolic disease characterised by chronic hyperglycaemia with impaired carbohydrate, fat and protein metabolism caused by defects in insulin secretion or action. Based on our previous research, stingless bee honey (SLBH) ... ...

Abstract	Diabetes mellitus (DM) is a metabolic disease characterised by chronic hyperglycaemia with impaired carbohydrate, fat and protein metabolism caused by defects in insulin secretion or action. Based on our previous research, stingless bee honey (SLBH) from
Language	English
Publishing date	2019-12-02
Publishing country	Saudi Arabia
Document type	Journal Article
ZDB-ID	2515206-3
ISSN	2213-7106 ; 1319-562X
ISSN (online)	2213-7106
ISSN	1319-562X
DOI	10.1016/j.sjbs.2019.11.039
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Molecular interaction analysis of Sulawesi propolis compounds with SARS-CoV-2 main protease as preliminary study for COVID-19 drug discovery.

Sahlan, Muhamad / Irdiani, Rafidha / Flamandita, Darin / Aditama, Reza / Alfarraj, Saleh / Ansari, Mohammad Javed / Khayrani, Apriliana Cahya / Pratami, Diah Kartika / Lischer, Kenny

Journal of King Saud University. Science

2020 Volume 33, Issue 1, Page(s) 101234

Abstract: Coronavirus disease 2019 (COVID-19), a respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a global health concern, as the World Health Organization declared this outbreak to be a global pandemic in March 2020. ... ...

Abstract	Coronavirus disease 2019 (COVID-19), a respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a global health concern, as the World Health Organization declared this outbreak to be a global pandemic in March 2020. The need for an effective treatment is urgent because the development of an effective vaccine may take years given the complexity of the virus and its rapid mutation. One promising treatment target for COVID-19 is SARS-CoV-2 main protease. Thus, this study was aimed to examine whether Sulawesi propolis compounds produced by
Keywords	covid19
Language	English
Publishing date	2020-11-17
Publishing country	Saudi Arabia
Document type	Journal Article
ISSN	2213-686X
ISSN (online)	2213-686X
DOI	10.1016/j.jksus.2020.101234
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Antiviral activity of

Wijanarko, Anondho / Lischer, Kenny / Hermansyah, Heri / Pratami, Diah Kartika / Sahlan, Muhamad

Veterinary world

2018 Volume 11, Issue 6, Page(s) 824–829

Abstract: Aim: Investigation of antiviral activity of : Materials and methods: Crude venom (CV) and F20 (PLA2 with 20% fractioned by ammonium sulfate) as a sample of PLA 2 obtained from : Results: AP-PLA2 F20 had activity and purity by 15.66 times bigger ... ...

Abstract	Aim: Investigation of antiviral activity of Materials and methods: Crude venom (CV) and F20 (PLA2 with 20% fractioned by ammonium sulfate) as a sample of PLA 2 obtained from Results: AP-PLA2 F20 had activity and purity by 15.66 times bigger than CV. The test showed that the LC Conclusion: This research suggests that AP-PLA2 has the potential to develop as an antiviral agent because
Language	English
Publishing date	2018-06-20
Publishing country	India
Document type	Journal Article
ZDB-ID	2456277-4
ISSN	2231-0916 ; 0972-8988
ISSN (online)	2231-0916
ISSN	0972-8988
DOI	10.14202/vetworld.2018.824-829
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Antiretroviral activity of

Sommeng, Andy Noorsaman / Arya, R Muhammad Yusuf / Ginting, Mikael Januardi / Pratami, Diah Kartika / Hermansyah, Heri / Sahlan, Muhamad / Wijanarko, Anondho

Veterinary world

2019 Volume 12, Issue 2, Page(s) 309–315

Abstract: Aim: This study aimed to investigate the antiviral activity of : Materials and methods: Fresh venomous fin parts of wild PV specimens were collected from Java Sea waters. Then, it washed using phosphate buffer pH 7.0 and immersed in phosphate buffer ... ...

Abstract	Aim: This study aimed to investigate the antiviral activity of Materials and methods: Fresh venomous fin parts of wild PV specimens were collected from Java Sea waters. Then, it washed using phosphate buffer pH 7.0 and immersed in phosphate buffer pH 7.0 0.01 m containing CaCl Results: The protein's activity, concentration, and purity analysis revealed that the PV-PLA2 purified using ammonium sulfate fractionation has the highest activity (1.81 times higher than the CV at 80% fractionation) and has higher purity than the sample from ET fractionation. The testing of the sample purified using ammonium sulfate fractionation at 80% saturation level shown that it has a 97.78% inhibition level toward SRV2-A549 culture at 4 ppm. However, in comparison to lamivudine which has 99.55% inhibition level at 100 ppm, it needs much lower concentration to achieve the same result. Conclusion: The significant inhibition of SRV2-A549 culture shown that the PV-PLA2 extracted from PV venom has the potential to become anti-HIV substances. It would be worthwhile to further evaluate the antiretroviral activity of PV-PLA2 in the
Language	English
Publishing date	2019-02-23
Publishing country	India
Document type	Journal Article
ZDB-ID	2456277-4
ISSN	2231-0916 ; 0972-8988
ISSN (online)	2231-0916
ISSN	0972-8988
DOI	10.14202/vetworld.2019.309-315
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Identification and classification of honey's authenticity by attenuated total reflectance Fourier-transform infrared spectroscopy and chemometric method.

Sahlan, Muhamad / Karwita, Seffiani / Gozan, Misri / Hermansyah, Heri / Yohda, Masafumi / Yoo, Young Je / Pratami, Diah Kartika

Veterinary world

2019 Volume 12, Issue 8, Page(s) 1304–1310

Abstract: Background and aim: The authentication of honey is important to protect industry and consumers from such adulterated honey. However, until now, there has been no guarantee of honey's authenticity, especially in Indonesia. The classification of honey is ... ...

Abstract	Background and aim: The authentication of honey is important to protect industry and consumers from such adulterated honey. However, until now, there has been no guarantee of honey's authenticity, especially in Indonesia. The classification of honey is based on the bee species (spp.) that produces it. The study used honey from sting bee Materials and methods: In this study, we use attenuated total reflectance Fourier-transform infrared (ATR-FTIR) spectroscopy with wavelengths ranging between 550 and 4000 cm Results: Our method effectively classified the honey as authentic or fraudulent based on the FTIR spectra. To authenticate the honey, we formed two classes: Real honey and fake honey. The wavelengths that can best differentiate between these two classes correspond to four regions: 1600-1700 cm Conclusion: This study successfully demonstrated a method to rapidly and accurately classify and authenticate honey. ATR-FTIR is a useful tool to test the authenticity of honey.
Language	English
Publishing date	2019-08-23
Publishing country	India
Document type	Journal Article
ZDB-ID	2456277-4
ISSN	2231-0916 ; 0972-8988
ISSN (online)	2231-0916
ISSN	0972-8988
DOI	10.14202/vetworld.2019.1304-1310
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