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  1. Article ; Online: Ligand and Carbohydrate Engagement (LACE) Assay and Fluorescence Quantification on Murine Neural Tissue.

    Clegg, James M / Pratt, Thomas

    Bio-protocol

    2021  Volume 11, Issue 6, Page(s) e3952

    Abstract: The interaction between cell surface heparan sulphate and diffusible ligands such as FGFs is of vital importance for downstream signaling, however, there are few techniques that can be used to investigate this binding event. The ligand and carbohydrate ... ...

    Abstract The interaction between cell surface heparan sulphate and diffusible ligands such as FGFs is of vital importance for downstream signaling, however, there are few techniques that can be used to investigate this binding event. The ligand and carbohydrate engagement (LACE) assay is a powerful tool which can be used to probe the molecular interaction between heparan sulphate and diffusible ligands and can detect changes in binding that may occur following genetic or pharmacological intervention. In this protocol we describe an FGF17:FGFR1 LACE assay performed on embryonic mouse brain tissue. We also describe the method we have used to quantify changes in fluorescent LACE signal in response to altered HS sulphation.
    Language English
    Publishing date 2021-03-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2833269-6
    ISSN 2331-8325 ; 2331-8325
    ISSN (online) 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.3952
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Commentary on "Infants With Congenital Muscular Torticollis: Demographic Factors, Clinical Characteristics, and Physical Therapy Episode of Care".

    Pratt, Tara / Coulter, Colleen Patricia / Mullally, Elizabeth Libby

    Pediatric physical therapy : the official publication of the Section on Pediatrics of the American Physical Therapy Association

    2022  Volume 34, Issue 3, Page(s) 352

    MeSH term(s) Demography ; Episode of Care ; Humans ; Infant ; Muscular Diseases ; Physical Therapy Modalities ; Torticollis/congenital ; Torticollis/therapy
    Language English
    Publishing date 2022-07-15
    Publishing country United States
    Document type Journal Article ; Comment
    ZDB-ID 1036679-9
    ISSN 1538-005X ; 0898-5669
    ISSN (online) 1538-005X
    ISSN 0898-5669
    DOI 10.1097/PEP.0000000000000931
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 2712: Show issues Location:
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  3. Article ; Online: Organotypic Slice Culture of the Embryonic Mouse Brain.

    Clegg, James M / Pratt, Thomas

    Bio-protocol

    2020  Volume 10, Issue 13, Page(s) e3674

    Abstract: Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays and ... ...

    Abstract Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays and bead culture assays.
    Language English
    Publishing date 2020-07-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2833269-6
    ISSN 2331-8325 ; 2331-8325
    ISSN (online) 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.3674
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: 16p11.2 deletion accelerates subpallial maturation and increases variability in human iPSC-derived ventral telencephalic organoids.

    Fetit, Rana / Barbato, Michela Ilaria / Theil, Thomas / Pratt, Thomas / Price, David J

    Development (Cambridge, England)

    2023  Volume 150, Issue 4

    Abstract: Inhibitory interneurons regulate cortical circuit activity, and their dysfunction has been implicated in autism spectrum disorder (ASD). 16p11.2 microdeletions are genetically linked to 1% of ASD cases. However, few studies investigate the effects of ... ...

    Abstract Inhibitory interneurons regulate cortical circuit activity, and their dysfunction has been implicated in autism spectrum disorder (ASD). 16p11.2 microdeletions are genetically linked to 1% of ASD cases. However, few studies investigate the effects of this microdeletion on interneuron development. Using ventral telencephalic organoids derived from human induced pluripotent stem cells, we have investigated the effect of this microdeletion on organoid size, progenitor proliferation and organisation into neural rosettes, ganglionic eminence marker expression at early developmental timepoints, and expression of the neuronal marker NEUN at later stages. At early stages, deletion organoids exhibited greater variations in size with concomitant increases in relative neural rosette area and the expression of the ventral telencephalic marker COUPTFII, with increased variability in these properties. Cell cycle analysis revealed an increase in total cell cycle length caused primarily by an elongated G1 phase, the duration of which also varied more than normal. At later stages, deletion organoids increased their NEUN expression. We propose that 16p11.2 microdeletions increase developmental variability and may contribute to ASD aetiology by lengthening the cell cycle of ventral progenitors, promoting premature differentiation into interneurons.
    MeSH term(s) Humans ; Induced Pluripotent Stem Cells ; Autism Spectrum Disorder/metabolism ; Telencephalon ; Neurons/metabolism ; Interneurons/metabolism ; Organoids
    Language English
    Publishing date 2023-02-24
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 90607-4
    ISSN 1477-9129 ; 0950-1991
    ISSN (online) 1477-9129
    ISSN 0950-1991
    DOI 10.1242/dev.201227
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    Zs.MG 91: Show issues

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  5. Article: Meconium peritonitis.

    PRATT, T L

    The Journal of the Faculty of Radiologists. Faculty of Radiologists (Great Britain)

    2014  Volume 5, Issue 1, Page(s) 62–64

    MeSH term(s) Child ; Fetal Diseases ; Humans ; Infant ; Infant, Newborn, Diseases ; Meconium ; Peritonitis
    Language English
    Publishing date 2014-02-11
    Publishing country England
    Document type Journal Article
    DOI 10.1016/s0368-2242(53)80038-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Spontaneous dislocation of the atlanto-axial articularion occurring in ankylosing spondylitis and rheumatoid arthritis.

    PRATT, T L

    The Journal of the Faculty of Radiologists. Faculty of Radiologists (Great Britain)

    2014  Volume 10, Issue 1, Page(s) 40–43

    MeSH term(s) Arthritis, Rheumatoid/complications ; Axis, Cervical Vertebra ; Cervical Atlas ; Humans ; Joint Dislocations ; Spondylitis, Ankylosing/complications
    Language English
    Publishing date 2014-02-11
    Publishing country England
    Document type Journal Article
    DOI 10.1016/s0368-2242(59)80010-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Evaluation and management of defecatory dysfunction in women.

    Pratt, Toya / Mishra, Kavita

    Current opinion in obstetrics & gynecology

    2018  Volume 30, Issue 6, Page(s) 451–457

    Abstract: Purpose of review: To summarize the current recommendations for the evaluation and management of defecatory dysfunction in women and highlight key relationships between defecatory dysfunction and other pelvic floor disorders, including pelvic organ ... ...

    Abstract Purpose of review: To summarize the current recommendations for the evaluation and management of defecatory dysfunction in women and highlight key relationships between defecatory dysfunction and other pelvic floor disorders, including pelvic organ prolapse, fecal incontinence, and voiding dysfunction.
    Recent findings: Conservative measures including lifestyle modifications, pharmacotherapy, and biofeedback continue to be the mainstay of treatment with newer therapies emerging. Physiologic testing and/or radiologic imaging should be considered for those who fail conservative therapy or are clinically complex. Surgical management is appropriate for carefully selected patients with anatomic causes of defecatory dysfunction. Further research is needed on surgical outcomes and patient expectations.
    Summary: Pelvic floor disorders, including defecatory dysfunction, have a significant societal impact and are highly prevalent among women. Given its potential complexity, a broader focus is needed when evaluating women with defecatory symptoms and effective treatment may require multidisciplinary care.
    MeSH term(s) Colorectal Surgery ; Constipation/physiopathology ; Constipation/therapy ; Fecal Incontinence/physiopathology ; Fecal Incontinence/therapy ; Female ; Humans ; Pelvic Floor/physiopathology ; Pelvic Floor Disorders/diagnosis ; Pelvic Floor Disorders/physiopathology ; Pelvic Floor Disorders/therapy ; Prevalence ; Quality of Life ; Treatment Outcome
    Language English
    Publishing date 2018-10-05
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1049382-7
    ISSN 1473-656X ; 1040-872X
    ISSN (online) 1473-656X
    ISSN 1040-872X
    DOI 10.1097/GCO.0000000000000495
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3048: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  8. Article ; Online: Expression of Genes in the 16p11.2 Locus during Development of the Human Fetal Cerebral Cortex.

    Morson, Sarah / Yang, Yifei / Price, David J / Pratt, Thomas

    Cerebral cortex (New York, N.Y. : 1991)

    2021  Volume 31, Issue 9, Page(s) 4038–4052

    Abstract: The 593 kbp 16p11.2 copy number variation (CNV) affects the gene dosage of 29 protein coding genes, with heterozygous 16p11.2 microduplication or microdeletion implicated in about 1% of autism spectrum disorder (ASD) cases. The 16p11.2 CNV is frequently ... ...

    Abstract The 593 kbp 16p11.2 copy number variation (CNV) affects the gene dosage of 29 protein coding genes, with heterozygous 16p11.2 microduplication or microdeletion implicated in about 1% of autism spectrum disorder (ASD) cases. The 16p11.2 CNV is frequently associated with macrocephaly or microcephaly indicating early defects of neurogenesis may contribute to subsequent ASD symptoms, but it is unknown which 16p11.2 transcripts are expressed in progenitors and whose levels are likely, therefore, to influence neurogenesis. Analysis of human fetal gene expression data revealed that KIF22, ALDOA, HIRIP3, PAGR1, and MAZ transcripts are expressed in neural progenitors with ALDOA and KIF22 significantly enriched compared to post-mitotic cells. To investigate the possible roles of ALDOA and KIF22 proteins in human cerebral cortex development we used immunohistochemical staining to describe their expression in late first and early second trimester human cerebral cortex. KIF22 protein is restricted to proliferating cells with its levels increasing during the cell cycle and peaking at mitosis. ALDOA protein is expressed in all cell types and does not vary with cell-cycle phase. Our expression analysis suggests the hypothesis that altered neurogenesis in the cerebral cortex contributes to ASD in 16p11.2 CNV patients.
    MeSH term(s) Adult ; Autism Spectrum Disorder/genetics ; Autism Spectrum Disorder/metabolism ; Cell Cycle ; Cerebral Cortex/growth & development ; Cerebral Cortex/metabolism ; DNA Copy Number Variations ; Female ; Fetus/metabolism ; Gene Deletion ; Gene Duplication ; Gene Expression Regulation/genetics ; Humans ; Immunohistochemistry ; Neural Stem Cells/metabolism ; Pregnancy
    Language English
    Publishing date 2021-04-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1077450-6
    ISSN 1460-2199 ; 1047-3211
    ISSN (online) 1460-2199
    ISSN 1047-3211
    DOI 10.1093/cercor/bhab067
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 3235: Show issues Location:
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  9. Article ; Online: Pax6 loss alters the morphological and electrophysiological development of mouse prethalamic neurons.

    Tian, Tian / Quintana-Urzainqui, Idoia / Kozić, Zrinko / Pratt, Thomas / Price, David J

    Development (Cambridge, England)

    2022  Volume 149, Issue 6

    Abstract: Pax6 is a well-known regulator of early neuroepithelial progenitor development. Its constitutive loss has a particularly strong effect on the developing prethalamus, causing it to become extremely hypoplastic. To overcome this difficulty in studying the ... ...

    Abstract Pax6 is a well-known regulator of early neuroepithelial progenitor development. Its constitutive loss has a particularly strong effect on the developing prethalamus, causing it to become extremely hypoplastic. To overcome this difficulty in studying the long-term consequences of Pax6 loss for prethalamic development, we used conditional mutagenesis to delete Pax6 at the onset of neurogenesis and studied the developmental potential of the mutant prethalamic neurons in vitro. We found that Pax6 loss affected their rates of neurite elongation, the location and length of their axon initial segments, and their electrophysiological properties. Our results broaden our understanding of the long-term consequences of Pax6 deletion in the developing mouse forebrain, suggesting that it can have cell-autonomous effects on the structural and functional development of some neurons.
    MeSH term(s) Animals ; Eye Proteins/metabolism ; Gene Expression Regulation, Developmental ; Homeodomain Proteins/metabolism ; Mice ; Neurons/metabolism ; PAX6 Transcription Factor/genetics ; Paired Box Transcription Factors/metabolism ; Repressor Proteins/metabolism
    Chemical Substances Eye Proteins ; Homeodomain Proteins ; PAX6 Transcription Factor ; Paired Box Transcription Factors ; Pax6 protein, mouse ; Repressor Proteins
    Language English
    Publishing date 2022-03-17
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 90607-4
    ISSN 1477-9129 ; 0950-1991
    ISSN (online) 1477-9129
    ISSN 0950-1991
    DOI 10.1242/dev.200052
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  10. Book: Woods and water

    Pratt, Timothy

    2014  

    Author's details Timothy Pratt, writer
    Keywords Forests and forestry/Study and teaching (Elementary) ; Water/Study and teaching/Activity programs.
    Language English
    Size 52 pages :, illustrations ;, 28 cm
    Document type Book
    Note Cover title.
    Database NAL-Catalogue (AGRICOLA)

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