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  1. Article: Characterization of the TRPV6 calcium channel-specific phenotype by RNA-seq in castration-resistant human prostate cancer cells.

    Cordier, Clément / Haustrate, Aurélien / Prevarskaya, Natalia / Lehen'kyi, V'yacheslav

    Frontiers in genetics

    2023  Volume 14, Page(s) 1215645

    Abstract: Background: ...

    Abstract Background:
    Language English
    Publishing date 2023-07-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606823-0
    ISSN 1664-8021
    ISSN 1664-8021
    DOI 10.3389/fgene.2023.1215645
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  2. Article ; Online: Ca

    Audero, Madelaine Magalì / Prevarskaya, Natalia / Fiorio Pla, Alessandra

    International journal of molecular sciences

    2022  Volume 23, Issue 13

    Abstract: Solid tumours are characterised by an altered microenvironment (TME) from the physicochemical point of view, displaying a highly hypoxic and acidic interstitial fluid. Hypoxia results from uncontrolled proliferation, aberrant vascularization and altered ... ...

    Abstract Solid tumours are characterised by an altered microenvironment (TME) from the physicochemical point of view, displaying a highly hypoxic and acidic interstitial fluid. Hypoxia results from uncontrolled proliferation, aberrant vascularization and altered cancer cell metabolism. Tumour cellular apparatus adapts to hypoxia by altering its metabolism and behaviour, increasing its migratory and metastatic abilities by the acquisition of a mesenchymal phenotype and selection of aggressive tumour cell clones. Extracellular acidosis is considered a cancer hallmark, acting as a driver of cancer aggressiveness by promoting tumour metastasis and chemoresistance via the selection of more aggressive cell phenotypes, although the underlying mechanism is still not clear. In this context, Ca
    MeSH term(s) Acidosis ; Humans ; Hypoxia ; Neoplasms/metabolism ; Signal Transduction ; Tumor Microenvironment
    Language English
    Publishing date 2022-07-02
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms23137377
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  3. Article: TRPM7 Ion Channel: Oncogenic Roles and Therapeutic Potential in Breast Cancer.

    Cordier, Clément / Prevarskaya, Natalia / Lehen'kyi, V'yacheslav

    Cancers

    2021  Volume 13, Issue 24

    Abstract: The transient receptor potential melastatin-subfamily member 7 (TRPM7) is a divalent cations permeant channel but also has intrinsic serine/threonine kinase activity. It is ubiquitously expressed in normal tissues and studies have indicated that it ... ...

    Abstract The transient receptor potential melastatin-subfamily member 7 (TRPM7) is a divalent cations permeant channel but also has intrinsic serine/threonine kinase activity. It is ubiquitously expressed in normal tissues and studies have indicated that it participates in important physiological and pharmacological processes through its channel-kinase activity, such as calcium/magnesium homeostasis, phosphorylation of proteins involved in embryogenesis or the cellular process. Accumulating evidence has shown that TRPM7 is overexpressed in human pathologies including breast cancer. Breast cancer is the second leading cause of cancer death in women with an incidence rate increase of around 0.5% per year since 2004. The overexpression of TRPM7 may be associated with a poor prognosis in breast cancer patients, so more efforts are needed to research a new therapeutic target. TRPM7 regulates the levels of Ca
    Language English
    Publishing date 2021-12-16
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers13246322
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  4. Article: Store operated calcium channels in cancer progression.

    Shapovalov, George / Gordienko, Dmitri / Prevarskaya, Natalia

    International review of cell and molecular biology

    2021  Volume 363, Page(s) 123–168

    Abstract: In recent decades cancer emerged as one of the leading causes of death in the developed countries, with some types of cancer contributing to the top 10 causes of death on the list of the World Health Organization. Carcinogenesis, a malignant ... ...

    Abstract In recent decades cancer emerged as one of the leading causes of death in the developed countries, with some types of cancer contributing to the top 10 causes of death on the list of the World Health Organization. Carcinogenesis, a malignant transformation causing formation of tumors in normal tissues, is associated with changes in the cell cycle caused by suppression of signaling pathways leading to cell death and facilitation of those enhancing proliferation. Further progression of cancer, during which benign tumors acquire more aggressive phenotypes, is characterized by metastatic dissemination through the body driven by augmented motility and invasiveness of cancer cells. All these processes are associated with alterations in calcium homeostasis in cancer cells, which promote their proliferation, motility and invasion, and dissuade cell death or cell cycle arrest. Remodeling of store-operated calcium entry (SOCE), one of the major pathways regulating intracellular Ca
    MeSH term(s) Animals ; Calcium/metabolism ; Calcium Channels/metabolism ; Carcinogenesis/metabolism ; Humans ; Neoplasms/metabolism ; Neoplasms/pathology
    Chemical Substances Calcium Channels ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2021-03-30
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 2427220-6
    ISSN 1937-6448 ; 0074-7696
    ISSN 1937-6448 ; 0074-7696
    DOI 10.1016/bs.ircmb.2021.02.016
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    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.317: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  5. Article: Ion Channel Profiling in Prostate Cancer: Toward Cell Population-Specific Screening.

    Farfariello, Valerio / Prevarskaya, Natalia / Gkika, Dimitra

    Reviews of physiology, biochemistry and pharmacology

    2021  Volume 181, Page(s) 39–56

    Abstract: In the last three decades, a growing number of studies have implicated ion channels in all essential processes of prostate carcinogenesis, including cell proliferation, apoptosis, migration, and angiogenesis. The changes in the expression of individual ... ...

    Abstract In the last three decades, a growing number of studies have implicated ion channels in all essential processes of prostate carcinogenesis, including cell proliferation, apoptosis, migration, and angiogenesis. The changes in the expression of individual ion channels show a specific profile, making these proteins promising clinical biomarkers that may enable better molecular subtyping of the disease and lead to more rapid and accurate clinical decision-making. Expression profiles and channel function are mainly based on the tumoral tissue itself, in this case, the epithelial cancer cell population. To date, little data on the ion channel profile of the cancerous prostate stroma are available, even though tumor interactions with the microenvironment are crucial in carcinogenesis and each distinct population plays a specific role in tumor progression. In this review, we describe ion channel expression profiles specific for the distinct cell population of the tumor microenvironment (stromal, endothelial, neuronal, and neuroendocrine cell populations) and the technical approaches used for efficient separation and screening of these cell populations.
    MeSH term(s) Carcinogenesis ; Early Detection of Cancer ; Humans ; Ion Channels ; Male ; Prostatic Neoplasms/diagnosis ; Tumor Microenvironment
    Chemical Substances Ion Channels
    Language English
    Publishing date 2021-12-30
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 125106-5
    ISSN 1617-5786 ; 0303-4240
    ISSN (online) 1617-5786
    ISSN 0303-4240
    DOI 10.1007/112_2020_22
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    In stock of ZB MED Cologne/Königswinter

    Uc I Zs 90: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Western Blotting and Co-immunoprecipitation of Endogenous STIM/ORAI and Protein Partners.

    Dubois, Charlotte / Prevarskaya, Natalia

    Methods in molecular biology (Clifton, N.J.)

    2018  Volume 1843, Page(s) 107–113

    Abstract: The characterization of protein-protein interactions through methods such as co-immunoprecipitation, followed by Western blot analysis, is a crucial step in the understanding of protein functions and the biology of the cell. Since the discovery of ORAI ... ...

    Abstract The characterization of protein-protein interactions through methods such as co-immunoprecipitation, followed by Western blot analysis, is a crucial step in the understanding of protein functions and the biology of the cell. Since the discovery of ORAI and STIM proteins as component of store-operated channel (SOC), overexpressing systems have been used to demonstrate how ORAI and STIM can associate with physiological and pathological conditions. Here we describe a protocol allowing endogenous studies.
    MeSH term(s) Blotting, Western/methods ; Calcium/metabolism ; Calcium Channels/metabolism ; Carrier Proteins/metabolism ; Cell Line, Tumor ; Humans ; Immunoprecipitation/methods ; Protein Binding ; Protein Interaction Mapping/methods ; Stromal Interaction Molecules/metabolism
    Chemical Substances Calcium Channels ; Carrier Proteins ; Stromal Interaction Molecules ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2018-09-10
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-8704-7_9
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  7. Article ; Online: Role of the TRPV Channels in the Endoplasmic Reticulum Calcium Homeostasis.

    Haustrate, Aurélien / Prevarskaya, Natalia / Lehen'kyi, V'yacheslav

    Cells

    2020  Volume 9, Issue 2

    Abstract: It has been widely established that transient receptor potential vanilloid (TRPV) channels play a crucial role in calcium homeostasis in mammalian cells. Modulation of TRPV channels activity can modify their physiological function leading to some ... ...

    Abstract It has been widely established that transient receptor potential vanilloid (TRPV) channels play a crucial role in calcium homeostasis in mammalian cells. Modulation of TRPV channels activity can modify their physiological function leading to some diseases and disorders like neurodegeneration, pain, cancer, skin disorders, etc. It should be noted that, despite TRPV channels importance, our knowledge of the TRPV channels functions in cells is mostly limited to their plasma membrane location. However, some TRPV channels were shown to be expressed in the endoplasmic reticulum where their modulation by activators and/or inhibitors was demonstrated to be crucial for intracellular signaling. In this review, we have intended to summarize the poorly studied roles and functions of these channels in the endoplasmic reticulum.
    MeSH term(s) Animals ; Calcium/metabolism ; Endoplasmic Reticulum/metabolism ; Homeostasis ; Humans ; Models, Biological ; Transient Receptor Potential Channels/metabolism
    Chemical Substances Transient Receptor Potential Channels ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2020-01-28
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells9020317
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  8. Article ; Online: Role of the TRP Channels in Pancreatic Ductal Adenocarcinoma Development and Progression.

    Mesquita, Gonçalo / Prevarskaya, Natalia / Schwab, Albrecht / Lehen'kyi, V'yacheslav

    Cells

    2021  Volume 10, Issue 5

    Abstract: The transient receptor potential channels (TRPs) have been related to several different physiologies that range from a role in sensory physiology (including thermo- and osmosensation) to a role in some pathologies like cancer. The great diversity of ... ...

    Abstract The transient receptor potential channels (TRPs) have been related to several different physiologies that range from a role in sensory physiology (including thermo- and osmosensation) to a role in some pathologies like cancer. The great diversity of functions performed by these channels is represented by nine sub-families that constitute the TRP channel superfamily. From the mid-2000s, several reports have shown the potential role of the TRP channels in cancers of multiple origin. The pancreatic cancer is one of the deadliest cancers worldwide. Its prevalence is predicted to rise further. Disappointingly, the treatments currently used are ineffective. There is an urgency to find new ways to counter this disease and one of the answers may lie in the ion channels belonging to the superfamily of TRP channels. In this review, we analyse the existing knowledge on the role of TRP channels in the development and progression of pancreatic ductal adenocarcinoma (PDAC). The functions of these channels in other cancers are also considered. This might be of interest for an extrapolation to the pancreatic cancer in an attempt to identify potential therapeutic interventions.
    MeSH term(s) Animals ; Calcium/metabolism ; Calcium Signaling ; Carcinoma, Pancreatic Ductal/metabolism ; Carcinoma, Pancreatic Ductal/pathology ; Cations ; Cell Movement ; Cell Proliferation ; Disease Progression ; Gene Expression Regulation, Neoplastic ; Humans ; Ion Channels ; Mice ; Neoplasm Invasiveness ; Pancreatic Neoplasms/metabolism ; Pancreatic Neoplasms/pathology ; Phylogeny ; Prevalence ; Transient Receptor Potential Channels/physiology ; Tumor Microenvironment
    Chemical Substances Cations ; Ion Channels ; Transient Receptor Potential Channels ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2021-04-26
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells10051021
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  9. Article: TRPV6 Calcium Channel Targeting by Antibodies Raised against Extracellular Epitopes Induces Prostate Cancer Cell Apoptosis.

    Haustrate, Aurélien / Shapovalov, George / Spriet, Corentin / Cordier, Clément / Kondratskyi, Artem / Noyer, Lucile / Foulquier, François / Prevarskaya, Natalia / Lehen'kyi, V'yacheslav

    Cancers

    2023  Volume 15, Issue 6

    Abstract: The TRPV6 calcium channel is known to be up-regulated in various tumors. The efforts to target the TRPV6 channel in vivo are still ongoing to propose an effective therapy against cancer. Here, we report the generation of two antibodies raised against ... ...

    Abstract The TRPV6 calcium channel is known to be up-regulated in various tumors. The efforts to target the TRPV6 channel in vivo are still ongoing to propose an effective therapy against cancer. Here, we report the generation of two antibodies raised against extracellular epitopes corresponding to the extracellular loop between S1 and S2 (rb79) and the pore region (rb82). These antibodies generated a complex biphasic response with the transient activation of the TRPV6 channel. Store-operated calcium entry was consequently potentiated in the prostate cancer cell line LNCaP upon the treatment. Both rb79 and rb82 antibodies significantly decreased cell survival rate in a dose-dependent manner as compared to the control antibodies of the same isotype. This decrease was due to the enhanced cell death via apoptosis revealed using a sub-G1 peak in a cell cycle assay, TUNEL assay, and a Hoechst staining, having no effects in the PC3M
    Language English
    Publishing date 2023-03-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers15061825
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  10. Article ; Online: CRAC Channel Components Quantitative Expression (In Tissues and Cell Lines) Using qPCR.

    Dubois, Charlotte / Lehenkyi, Vyacheslav / Prevarskaya, Natalia

    Methods in molecular biology (Clifton, N.J.)

    2018  Volume 1843, Page(s) 95–106

    Abstract: Since last decade real-time qPCR has become a routine and robust approach for measuring the expression of genes of interest. Indeed, using qPCR, expression profile analyses are now possible and participate to the understanding of physiological or ... ...

    Abstract Since last decade real-time qPCR has become a routine and robust approach for measuring the expression of genes of interest. Indeed, using qPCR, expression profile analyses are now possible and participate to the understanding of physiological or pathological role of channels such as calcium release-activated channels (CRAC). Initially discovered in lymphocyte T and immunity perturbations, recent studies have highlighted the role of CRAC channels in other pathologies such as cancer. Here we describe a protocol to quantify CRAC components expression, in tissue sample and cell lines, to validate knockdown strategies or identify their roles in physiological and pathological conditions (Hoth and Penner, J Physiol 465:359-386, 1993; Hoth and Penner, Nature 355:353-356, 1992; and Zweifach and Lewis, Proc Natl Acad Sci U S A 90:6295-6299, 1993).
    MeSH term(s) Calcium/metabolism ; Calcium Release Activated Calcium Channels/genetics ; Calcium Release Activated Calcium Channels/metabolism ; Calcium Signaling ; Cell Line ; Gene Expression ; Humans ; Ion Channel Gating ; Real-Time Polymerase Chain Reaction/methods
    Chemical Substances Calcium Release Activated Calcium Channels ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2018-09-10
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-8704-7_8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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