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  1. Article ; Online: Gradients and consequences of heterogeneity in biofilms.

    Jo, Jeanyoung / Price-Whelan, Alexa / Dietrich, Lars E P

    Nature reviews. Microbiology

    2022  Volume 20, Issue 10, Page(s) 593–607

    Abstract: Historically, appreciation for the roles of resource gradients in biology has fluctuated inversely to the popularity of genetic mechanisms. Nevertheless, in microbiology specifically, widespread recognition of the multicellular lifestyle has recently ... ...

    Abstract Historically, appreciation for the roles of resource gradients in biology has fluctuated inversely to the popularity of genetic mechanisms. Nevertheless, in microbiology specifically, widespread recognition of the multicellular lifestyle has recently brought new emphasis to the importance of resource gradients. Most microorganisms grow in assemblages such as biofilms or spatially constrained communities with gradients that influence, and are influenced by, metabolism. In this Review, we discuss examples of gradient formation and physiological differentiation in microbial assemblages growing in diverse settings. We highlight consequences of physiological heterogeneity in microbial assemblages, including division of labour and increased resistance to stress. Our impressions of microbial behaviour in various ecosystems are not complete without complementary maps of the chemical and physical geographies that influence cellular activities. A holistic view, incorporating these geographies and the genetically encoded functions that operate within them, will be essential for understanding microbial assemblages in their many roles and potential applications.
    MeSH term(s) Biofilms ; Ecosystem
    Language English
    Publishing date 2022-02-11
    Publishing country England
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural
    ZDB-ID 2139054-X
    ISSN 1740-1534 ; 1740-1526
    ISSN (online) 1740-1534
    ISSN 1740-1526
    DOI 10.1038/s41579-022-00692-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5865: Show issues Location:
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    Zs.MG 74: Show issues

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  2. Article: Cyanide-dependent control of terminal oxidase hybridization by

    Smiley, Marina K / Sekaran, Doran C / Price-Whelan, Alexa / Dietrich, Lars E P

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Pseudomonas ... ...

    Abstract Pseudomonas aeruginosa
    Language English
    Publishing date 2023-06-20
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.05.31.543164
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Light/Dark and Temperature Cycling Modulate Metabolic Electron Flow in Pseudomonas aeruginosa Biofilms.

    Kahl, Lisa Juliane / Eckartt, Kelly N / Morales, Diana K / Price-Whelan, Alexa / Dietrich, Lars E P

    mBio

    2022  Volume 13, Issue 4, Page(s) e0140722

    Abstract: Sunlight drives phototrophic metabolism, which affects redox conditions and produces substrates for nonphototrophs. These environmental parameters fluctuate daily due to Earth's rotation, and nonphototrophic organisms can therefore benefit from the ... ...

    Abstract Sunlight drives phototrophic metabolism, which affects redox conditions and produces substrates for nonphototrophs. These environmental parameters fluctuate daily due to Earth's rotation, and nonphototrophic organisms can therefore benefit from the ability to respond to, or even anticipate, such changes. Circadian rhythms, such as daily changes in body temperature, in host organisms can also affect local conditions for colonizing bacteria. Here, we investigated the effects of light/dark and temperature cycling on biofilms of the opportunistic pathogen Pseudomonas aeruginosa PA14. We grew biofilms in the presence of a respiratory indicator dye and found that enhanced dye reduction occurred in biofilm zones that formed during dark intervals and at lower temperatures. This pattern formation occurred with cycling of blue, red, or far-red light, and a screen of mutants representing potential sensory proteins identified two with defects in pattern formation, specifically under red light cycling. We also found that the physiological states of biofilm subzones formed under specific light and temperature conditions were retained during subsequent condition cycling. Light/dark and temperature cycling affected expression of genes involved in primary metabolic pathways and redox homeostasis, including those encoding electron transport chain components. Consistent with this, we found that
    MeSH term(s) Animals ; Biofilms ; Electrons ; Oxidation-Reduction ; Pseudomonas aeruginosa/metabolism ; Temperature
    Language English
    Publishing date 2022-08-08
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2557172-2
    ISSN 2150-7511 ; 2161-2129
    ISSN (online) 2150-7511
    ISSN 2161-2129
    DOI 10.1128/mbio.01407-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: MpaR-driven expression of an orphan terminal oxidase subunit supports

    Smiley, Marina K / Sekaran, Doran C / Forouhar, Farhad / Wolin, Erica / Jovanovic, Marko / Price-Whelan, Alexa / Dietrich, Lars E P

    mBio

    2023  Volume 15, Issue 1, Page(s) e0292623

    Abstract: Importance: Cyanide is an inhibitor of heme-copper oxidases, which are required for aerobic respiration in all eukaryotes and many prokaryotes. This fast-acting poison can arise from diverse sources, but mechanisms by which bacteria sense it are poorly ... ...

    Abstract Importance: Cyanide is an inhibitor of heme-copper oxidases, which are required for aerobic respiration in all eukaryotes and many prokaryotes. This fast-acting poison can arise from diverse sources, but mechanisms by which bacteria sense it are poorly understood. We investigated the regulatory response to cyanide in the pathogenic bacterium
    MeSH term(s) Pseudomonas aeruginosa/metabolism ; Oxidoreductases/genetics ; Oxidoreductases/metabolism ; Cyanides/metabolism ; Respiration ; Biofilms ; Heme/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism
    Chemical Substances Oxidoreductases (EC 1.-) ; Cyanides ; Heme (42VZT0U6YR) ; Bacterial Proteins
    Language English
    Publishing date 2023-12-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2557172-2
    ISSN 2150-7511 ; 2161-2129
    ISSN (online) 2150-7511
    ISSN 2161-2129
    DOI 10.1128/mbio.02926-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Spatial heterogeneity in biofilm metabolism elicited by local control of phenazine methylation.

    Evans, Christopher R / Smiley, Marina K / Thio, Sean Asahara / Wei, Mian / Price-Whelan, Alexa / Min, Wei / Dietrich, Lars E P

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Within biofilms, gradients of electron acceptors such as oxygen stimulate the formation of physiological subpopulations. This heterogeneity can enable cross-feeding and promote drug resilience, features of the multicellular lifestyle that make biofilm- ... ...

    Abstract Within biofilms, gradients of electron acceptors such as oxygen stimulate the formation of physiological subpopulations. This heterogeneity can enable cross-feeding and promote drug resilience, features of the multicellular lifestyle that make biofilm-based infections difficult to treat. The pathogenic bacterium
    Language English
    Publishing date 2023-02-16
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.02.15.528762
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Cellular arrangement impacts metabolic activity and antibiotic tolerance in Pseudomonas aeruginosa biofilms.

    Dayton, Hannah / Kiss, Julie / Wei, Mian / Chauhan, Shradha / LaMarre, Emily / Cornell, William Cole / Morgan, Chase J / Janakiraman, Anuradha / Min, Wei / Tomer, Raju / Price-Whelan, Alexa / Nirody, Jasmine A / Dietrich, Lars E P

    PLoS biology

    2024  Volume 22, Issue 2, Page(s) e3002205

    Abstract: Cells must access resources to survive, and the anatomy of multicellular structures influences this access. In diverse multicellular eukaryotes, resources are provided by internal conduits that allow substances to travel more readily through tissue than ... ...

    Abstract Cells must access resources to survive, and the anatomy of multicellular structures influences this access. In diverse multicellular eukaryotes, resources are provided by internal conduits that allow substances to travel more readily through tissue than they would via diffusion. Microbes growing in multicellular structures, called biofilms, are also affected by differential access to resources and we hypothesized that this is influenced by the physical arrangement of the cells. In this study, we examined the microanatomy of biofilms formed by the pathogenic bacterium Pseudomonas aeruginosa and discovered that clonal cells form striations that are packed lengthwise across most of a mature biofilm's depth. We identified mutants, including those defective in pilus function and in O-antigen attachment, that show alterations to this lengthwise packing phenotype. Consistent with the notion that cellular arrangement affects access to resources within the biofilm, we found that while the wild type shows even distribution of tested substrates across depth, the mutants show accumulation of substrates at the biofilm boundaries. Furthermore, we found that altered cellular arrangement within biofilms affects the localization of metabolic activity, the survival of resident cells, and the susceptibility of subpopulations to antibiotic treatment. Our observations provide insight into cellular features that determine biofilm microanatomy, with consequences for physiological differentiation and drug sensitivity.
    MeSH term(s) Humans ; Anti-Bacterial Agents/pharmacology ; Pseudomonas aeruginosa/metabolism ; Biofilms ; Pseudomonas Infections/microbiology ; Fimbriae, Bacterial
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2024-02-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2126776-5
    ISSN 1545-7885 ; 1544-9173
    ISSN (online) 1545-7885
    ISSN 1544-9173
    DOI 10.1371/journal.pbio.3002205
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6193: Show issues Location:
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  7. Article: The L-lactate dehydrogenases of

    Florek, Lindsey C / Lin, Xi / Lin, Yu-Cheng / Lin, Min-Han / Chakraborty, Arijit / Price-Whelan, Alexa / Tong, Liang / Rahme, Laurence / Dietrich, Lars E P

    bioRxiv : the preprint server for biology

    2024  

    Abstract: Pseudomonas ... ...

    Abstract Pseudomonas aeruginosa
    Language English
    Publishing date 2024-03-23
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.03.21.586142
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Light-Mediated Decreases in Cyclic di-GMP Levels Inhibit Structure Formation in

    Kahl, Lisa Juliane / Price-Whelan, Alexa / Dietrich, Lars E P

    Journal of bacteriology

    2020  Volume 202, Issue 14

    Abstract: Light is known to trigger regulatory responses in diverse organisms, including slime molds, animals, plants, and phototrophic bacteria. However, light-dependent processes in nonphototrophic bacteria, and those of pathogens in particular, have received ... ...

    Abstract Light is known to trigger regulatory responses in diverse organisms, including slime molds, animals, plants, and phototrophic bacteria. However, light-dependent processes in nonphototrophic bacteria, and those of pathogens in particular, have received comparatively little research attention. In this study, we examined the impact of light on multicellular development in
    MeSH term(s) Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Biofilms/radiation effects ; Cyclic GMP/analogs & derivatives ; Cyclic GMP/metabolism ; Gene Expression Regulation, Bacterial/radiation effects ; Light ; Oxidation-Reduction ; Phenazines/metabolism ; Pseudomonas aeruginosa/genetics ; Pseudomonas aeruginosa/physiology ; Pseudomonas aeruginosa/radiation effects
    Chemical Substances Bacterial Proteins ; Phenazines ; bis(3',5')-cyclic diguanylic acid (61093-23-0) ; Cyclic GMP (H2D2X058MU)
    Language English
    Publishing date 2020-06-25
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/JB.00117-20
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.50: Show issues Location:
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  9. Article: Metabolic Heterogeneity and Cross-Feeding in Bacterial Multicellular Systems

    Evans, Christopher R / Kempes, Christopher P / Price-Whelan, Alexa / Dietrich, Lars E.P

    Trends in microbiology. 2020,

    2020  

    Abstract: Cells in assemblages differentiate and perform distinct roles. Though many pathways of differentiation are understood at the molecular level in multicellular eukaryotes, the elucidation of similar processes in bacterial assemblages is recent and ongoing. ...

    Abstract Cells in assemblages differentiate and perform distinct roles. Though many pathways of differentiation are understood at the molecular level in multicellular eukaryotes, the elucidation of similar processes in bacterial assemblages is recent and ongoing. Here, we discuss examples of bacterial differentiation, focusing on cases in which distinct metabolisms coexist and those that exhibit cross-feeding, with one subpopulation producing substrates that are metabolized by a second subpopulation. We describe several studies of single-species systems, then segue to studies of multispecies metabolic heterogeneity and cross-feeding in the clinical setting. Many of the studies described exemplify the application of new techniques and modeling approaches that provide insights into metabolic interactions relevant for bacterial growth outside the laboratory.
    Keywords bacterial growth ; eukaryotic cells ; models
    Language English
    Publishing place Elsevier Ltd
    Document type Article
    Note Pre-press version
    ZDB-ID 1158963-2
    ISSN 1878-4380 ; 0966-842X
    ISSN (online) 1878-4380
    ISSN 0966-842X
    DOI 10.1016/j.tim.2020.03.008
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 2005/714: Show issues

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  10. Article ; Online: Interdependency of Respiratory Metabolism and Phenazine-Associated Physiology in Pseudomonas aeruginosa PA14.

    Jo, Jeanyoung / Price-Whelan, Alexa / Cornell, William Cole / Dietrich, Lars E P

    Journal of bacteriology

    2020  Volume 202, Issue 4

    Abstract: Extracellular electron transfer (EET), the reduction of compounds that shuttle electrons to distal oxidants, can support bacterial survival when preferred oxidants are not directly accessible. EET has been shown to contribute to virulence in some ... ...

    Abstract Extracellular electron transfer (EET), the reduction of compounds that shuttle electrons to distal oxidants, can support bacterial survival when preferred oxidants are not directly accessible. EET has been shown to contribute to virulence in some pathogenic organisms and is required for current generation in mediator-based fuel cells. In several species, components of the electron transport chain (ETC) have been implicated in electron shuttle reduction, raising the question of how shuttling-based metabolism is integrated with primary routes of metabolic electron flow. The clinically relevant bacterium
    MeSH term(s) Biofilms ; Carbon/metabolism ; Cystic Fibrosis/microbiology ; Electron Transport ; Humans ; Oxidation-Reduction ; Phenazines/metabolism ; Pseudomonas aeruginosa/growth & development ; Pseudomonas aeruginosa/metabolism
    Chemical Substances Phenazines ; phenazine ; Carbon (7440-44-0)
    Language English
    Publishing date 2020-01-29
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/JB.00700-19
    Database MEDical Literature Analysis and Retrieval System OnLINE

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