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  1. Article: The Extracellular Milieu of

    Primo, Vincent A / Rezvani, Yasaman / Farrell, Andrew / Murphy, Connor Q / Lou, Jingjing / Vajdi, Amir / Marth, Gabor T / Zarringhalam, Kourosh / Gubbels, Marc-Jan

    mSystems

    2021  Volume 6, Issue 6, Page(s) e0119621

    Abstract: Evolve and resequencing (E&R) was applied to lab adaptation of Toxoplasma gondii for over 1,500 generations with the goal of mapping host- ... ...

    Abstract Evolve and resequencing (E&R) was applied to lab adaptation of Toxoplasma gondii for over 1,500 generations with the goal of mapping host-independent
    Language English
    Publishing date 2021-12-07
    Publishing country United States
    Document type Journal Article
    ISSN 2379-5077
    ISSN 2379-5077
    DOI 10.1128/mSystems.01196-21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Isolation and Transfection of Primary Culture Bovine Retinal Pericytes.

    Primo, Vincent A / Arboleda-Velasquez, Joseph F

    Methods in molecular biology (Clifton, N.J.)

    2016  Volume 1430, Page(s) 107–117

    Abstract: This protocol describes an enzymatic approach for isolating homogeneous cultures of pericytes from retinas of bovine source. In summary, retinas are dissected, washed, digested, filtered, cultured in specific media to select for pericytes, and finally ... ...

    Abstract This protocol describes an enzymatic approach for isolating homogeneous cultures of pericytes from retinas of bovine source. In summary, retinas are dissected, washed, digested, filtered, cultured in specific media to select for pericytes, and finally expanded for a low passage culture of about 14 million bovine retinal pericytes (BRP) within 4-6 weeks. This protocol also describes a liposomal-based technique for transfection of BRPs.
    Language English
    Publishing date 2016
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-3628-1_7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Toxoplasma gondii

    Gubbels, Marc-Jan / Ferguson, David J P / Saha, Sudeshna / Romano, Julia D / Chavan, Suyog / Primo, Vincent A / Michaud, Cynthia / Coppens, Isabelle / Engelberg, Klemens

    Frontiers in cellular and infection microbiology

    2022  Volume 12, Page(s) 882166

    Abstract: The Apicomplexa are famously named for their apical complex, a constellation of organelles at their apical end dedicated to invasion of their host cells. In contrast, at the other end of the cell, the basal complex (BC) has been overshadowed since it is ... ...

    Abstract The Apicomplexa are famously named for their apical complex, a constellation of organelles at their apical end dedicated to invasion of their host cells. In contrast, at the other end of the cell, the basal complex (BC) has been overshadowed since it is much less prominent and specific functions were not immediately obvious. However, in the past decade a staggering array of functions have been associated with the BC and strides have been made in understanding its structure. Here, these collective insights are supplemented with new data to provide an overview of the understanding of the BC in
    MeSH term(s) Cell Division ; Cytoskeleton/metabolism ; Organelles/metabolism ; Protozoan Proteins/genetics ; Toxoplasma/metabolism
    Chemical Substances Protozoan Proteins
    Language English
    Publishing date 2022-04-29
    Publishing country Switzerland
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2022.882166
    Database MEDical Literature Analysis and Retrieval System OnLINE

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