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Article ; Online: Photosynthesis and other factors affecting the establishment and maintenance of cnidarian-dinoflagellate symbiosis.

Tran, Cawa / Rosenfield, Gabriel R / Cleves, Phillip A / Krediet, Cory J / Paul, Maitri R / Clowez, Sophie / Grossman, Arthur R / Pringle, John R

Philosophical transactions of the Royal Society of London. Series B, Biological sciences

2024 Volume 379, Issue 1901, Page(s) 20230079

Abstract: Coral growth depends on the partnership between the animal hosts and their intracellular, photosynthetic dinoflagellate symbionts. In this study, we used the sea ... ...

Abstract	Coral growth depends on the partnership between the animal hosts and their intracellular, photosynthetic dinoflagellate symbionts. In this study, we used the sea anemone
MeSH term(s)	Animals ; Symbiosis ; Dinoflagellida ; Anthozoa ; Sea Anemones ; Photosynthesis ; Larva
Language	English
Publishing date	2024-03-18
Publishing country	England
Document type	Journal Article
ZDB-ID	208382-6
ISSN	1471-2970 ; 0080-4622 ; 0264-3839 ; 0962-8436
ISSN (online)	1471-2970
ISSN	0080-4622 ; 0264-3839 ; 0962-8436
DOI	10.1098/rstb.2023.0079
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Article ; Online: An enduring enthusiasm for academic science, but with concerns.

Pringle, John R

Molecular biology of the cell

2013 Volume 24, Issue 21, Page(s) 3281–3284

MeSH term(s)	History, 20th Century ; History, 21st Century ; Research/history ; Science/history ; Teaching/history ; United States ; Universities
Language	English
Publishing date	2013-10-30
Publishing country	United States
Document type	Autobiography ; Biography ; Historical Article ; Journal Article ; Portrait
ZDB-ID	1098979-1
ISSN	1939-4586 ; 1059-1524
ISSN (online)	1939-4586
ISSN	1059-1524
DOI	10.1091/mbc.E13-07-0393
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Zs.A 2853: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Impact of Menthol on Growth and Photosynthetic Function of Breviolum Minutum (Dinoflagellata, Dinophyceae, Symbiodiniaceae) and Interactions with its Aiptasia Host.

Clowez, Sophie / Renicke, Christian / Pringle, John R / Grossman, Arthur R

Journal of phycology

2021 Volume 57, Issue 1, Page(s) 245–257

Abstract: Environmental change, including global warming and chemical pollution, can compromise cnidarian-(e.g., coral-) dinoflagellate symbioses and cause coral bleaching. Understanding the mechanisms that regulate these symbioses will inform strategies for ... ...

Abstract	Environmental change, including global warming and chemical pollution, can compromise cnidarian-(e.g., coral-) dinoflagellate symbioses and cause coral bleaching. Understanding the mechanisms that regulate these symbioses will inform strategies for sustaining healthy coral-reef communities. A model system for corals is the symbiosis between the sea anemone Exaiptasia pallida (common name Aiptasia) and its dinoflagellate partners (family Symbiodiniaceae). To complement existing studies of the interactions between these organisms, we examined the impact of menthol, a reagent often used to render cnidarians aposymbiotic, on the dinoflagellate Breviolum minutum, both in culture and in hospite. In both environments, the growth and photosynthesis of this alga were compromised at either 100 or 300 µM menthol. We observed reduction in PSII and PSI functions, the abundances of reaction-center proteins, and, at 300 µM menthol, of total cellular proteins. Interestingly, for free-living algae exposed to 100 µM menthol, an initial decline in growth, photosynthetic activities, pigmentation, and protein abundances reversed after 5-15 d, eventually approaching control levels. This behavior was observed in cells maintained in continuous light, but not in cells experiencing a light-dark regimen, suggesting that B. minutum can detoxify menthol or acclimate and repair damaged photosynthetic complexes in a light- and/or energy-dependent manner. Extended exposures of cultured algae to 300 µM menthol ultimately resulted in algal death. Most symbiotic anemones were also unable to survive this menthol concentration for 30 d. Additionally, cells impaired for photosynthesis by pre-treatment with 300 µM menthol exhibited reduced efficiency in re-populating the anemone host.
MeSH term(s)	Animals ; Dinoflagellida ; Menthol ; Photosynthesis ; Sea Anemones ; Symbiosis
Chemical Substances	Menthol (1490-04-6)
Language	English
Publishing date	2021-01-19
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	281226-5
ISSN	1529-8817 ; 0022-3646
ISSN (online)	1529-8817
ISSN	0022-3646
DOI	10.1111/jpy.13081
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Article ; Online: Conversion of oxybenzone sunscreen to phototoxic glucoside conjugates by sea anemones and corals.

Vuckovic, Djordje / Tinoco, Amanda I / Ling, Lorraine / Renicke, Christian / Pringle, John R / Mitch, William A

Science (New York, N.Y.)

2022 Volume 376, Issue 6593, Page(s) 644–648

Abstract: The reported toxicity of oxybenzone-based sunscreens to corals has raised concerns about the impacts of ecotourist-shed sunscreens on corals already weakened by global stressors. However, oxybenzone's toxicity mechanism(s) are not understood, hampering ... ...

Abstract	The reported toxicity of oxybenzone-based sunscreens to corals has raised concerns about the impacts of ecotourist-shed sunscreens on corals already weakened by global stressors. However, oxybenzone's toxicity mechanism(s) are not understood, hampering development of safer sunscreens. We found that oxybenzone caused high mortality of a sea anemone under simulated sunlight including ultraviolet (UV) radiation (290 to 370 nanometers). Although oxybenzone itself protected against UV-induced photo-oxidation, both the anemone and a mushroom coral formed oxybenzone-glucoside conjugates that were strong photo-oxidants. Algal symbionts sequestered these conjugates, and mortality correlated with conjugate concentrations in animal cytoplasm. Higher mortality in anemones that lacked symbionts suggests an enhanced risk from oxybenzone to corals bleached by rising temperatures. Because many commercial sunscreens contain structurally related chemicals, understanding metabolite phototoxicity should facilitate the development of coral-safe products.
MeSH term(s)	Animals ; Anthozoa ; Benzophenones ; Glucosides/toxicity ; Sea Anemones ; Sunscreening Agents/toxicity
Chemical Substances	Benzophenones ; Glucosides ; Sunscreening Agents ; oxybenzone (95OOS7VE0Y)
Language	English
Publishing date	2022-05-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	128410-1
ISSN	1095-9203 ; 0036-8075
ISSN (online)	1095-9203
ISSN	0036-8075
DOI	10.1126/science.abn2600
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Zs.A 27: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Role of the bicarbonate transporter SLC4γ in stony-coral skeleton formation and evolution.

Tinoco, Amanda I / Mitchison-Field, Lorna M Y / Bradford, Jacob / Renicke, Christian / Perrin, Dimitri / Bay, Line K / Pringle, John R / Cleves, Phillip A

Proceedings of the National Academy of Sciences of the United States of America

2023 Volume 120, Issue 24, Page(s) e2216144120

Abstract: Coral reefs are highly diverse ecosystems of immense ecological, economic, and aesthetic importance built on the calcium-carbonate-based skeletons of stony corals. The formation of these skeletons is threatened by increasing ocean temperatures and ... ...

Abstract	Coral reefs are highly diverse ecosystems of immense ecological, economic, and aesthetic importance built on the calcium-carbonate-based skeletons of stony corals. The formation of these skeletons is threatened by increasing ocean temperatures and acidification, and a deeper understanding of the molecular mechanisms involved may assist efforts to mitigate the effects of such anthropogenic stressors. In this study, we focused on the role of the predicted bicarbonate transporter SLC4γ, which was suggested in previous studies to be a product of gene duplication and to have a role in coral-skeleton formation. Our comparative-genomics study using 30 coral species and 15 outgroups indicates that SLC4γ is present throughout the stony corals, but not in their non-skeleton-forming relatives, and apparently arose by gene duplication at the onset of stony-coral evolution. Our expression studies show that
MeSH term(s)	Animals ; Anthozoa/genetics ; Bicarbonates ; Ecosystem ; Calcium ; Coral Reefs
Chemical Substances	Bicarbonates ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2023-06-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	209104-5
ISSN	1091-6490 ; 0027-8424
ISSN (online)	1091-6490
ISSN	0027-8424
DOI	10.1073/pnas.2216144120
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Article: Impact of Menthol on Growth and Photosynthetic Function of Breviolum Minutum (Dinoflagellata, Dinophyceae, Symbiodiniaceae) and Interactions with its Aiptasia Host

Clowez, Sophie / Renicke, Christian / Pringle, John R / Grossman, Arthur R

Journal of phycology. 2021 Feb., v. 57, no. 1

2021

Abstract: Environmental change, including global warming and chemical pollution, can compromise cnidarian‐(e.g., coral‐) dinoflagellate symbioses and cause coral bleaching. Understanding the mechanisms that regulate these symbioses will inform strategies for ... ...

Abstract	Environmental change, including global warming and chemical pollution, can compromise cnidarian‐(e.g., coral‐) dinoflagellate symbioses and cause coral bleaching. Understanding the mechanisms that regulate these symbioses will inform strategies for sustaining healthy coral–reef communities. A model system for corals is the symbiosis between the sea anemone Exaiptasia pallida (common name Aiptasia) and its dinoflagellate partners (family Symbiodiniaceae). To complement existing studies of the interactions between these organisms, we examined the impact of menthol, a reagent often used to render cnidarians aposymbiotic, on the dinoflagellate Breviolum minutum, both in culture and in hospite. In both environments, the growth and photosynthesis of this alga were compromised at either 100 or 300 µM menthol. We observed reduction in PSII and PSI functions, the abundances of reaction‐center proteins, and, at 300 µM menthol, of total cellular proteins. Interestingly, for free‐living algae exposed to 100 µM menthol, an initial decline in growth, photosynthetic activities, pigmentation, and protein abundances reversed after 5–15 d, eventually approaching control levels. This behavior was observed in cells maintained in continuous light, but not in cells experiencing a light–dark regimen, suggesting that B. minutum can detoxify menthol or acclimate and repair damaged photosynthetic complexes in a light‐ and/or energy‐dependent manner. Extended exposures of cultured algae to 300 µM menthol ultimately resulted in algal death. Most symbiotic anemones were also unable to survive this menthol concentration for 30 d. Additionally, cells impaired for photosynthesis by pre‐treatment with 300 µM menthol exhibited reduced efficiency in re‐populating the anemone host.
Keywords	Anthozoa ; Symbiodiniaceae ; algae ; algology ; common names ; corals ; death ; menthol ; photosystem II ; pigmentation ; pollution ; symbiosis
Language	English
Dates of publication	2021-02
Size	p. 245-257.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	NAL-AP-2-clean ; JOURNAL ARTICLE
ZDB-ID	281226-5
ISSN	1529-8817 ; 0022-3646
ISSN (online)	1529-8817
ISSN	0022-3646
DOI	10.1111/jpy.13081
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Cleavage-furrow formation without F-actin in

Onishi, Masayuki / Umen, James G / Cross, Frederick R / Pringle, John R

Proceedings of the National Academy of Sciences of the United States of America

2020 Volume 117, Issue 31, Page(s) 18511–18520

Abstract: It is widely believed that cleavage-furrow formation during cytokinesis is driven by the contraction of a ring containing F-actin and type-II myosin. However, even in cells that have such rings, they are not always essential for furrow formation. ... ...

Abstract	It is widely believed that cleavage-furrow formation during cytokinesis is driven by the contraction of a ring containing F-actin and type-II myosin. However, even in cells that have such rings, they are not always essential for furrow formation. Moreover, many taxonomically diverse eukaryotic cells divide by furrowing but have no type-II myosin, making it unlikely that an actomyosin ring drives furrowing. To explore this issue further, we have used one such organism, the green alga
MeSH term(s)	Actin Cytoskeleton/chemistry ; Actin Cytoskeleton/metabolism ; Actins/chemistry ; Actins/metabolism ; Cell Division ; Chlamydomonas/chemistry ; Chlamydomonas/cytology ; Chlamydomonas/metabolism ; Cytokinesis ; Microtubules/metabolism ; Myosins/chemistry ; Myosins/metabolism ; Protein Binding
Chemical Substances	Actins ; Myosins (EC 3.6.4.1)
Language	English
Publishing date	2020-07-20
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	209104-5
ISSN	1091-6490 ; 0027-8424
ISSN (online)	1091-6490
ISSN	0027-8424
DOI	10.1073/pnas.1920337117
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Article ; Online: Robust Transgene Expression from Bicistronic mRNA in the Green Alga Chlamydomonas reinhardtii.

Onishi, Masayuki / Pringle, John R

G3 (Bethesda, Md.)

2016 Volume 6, Issue 12, Page(s) 4115–4125

Abstract: The unicellular green alga Chlamydomonas reinhardtii is a model organism that provides an opportunity to understand the evolution and functional biology of the lineage that includes the land plants, as well as aspects of the fundamental core biology ... ...

Abstract	The unicellular green alga Chlamydomonas reinhardtii is a model organism that provides an opportunity to understand the evolution and functional biology of the lineage that includes the land plants, as well as aspects of the fundamental core biology conserved throughout the eukaryotic phylogeny. Although many tools are available to facilitate genetic, molecular biological, biochemical, and cell biological studies in Chlamydomonas, expression of unselected transgenes of interest (GOIs) has been challenging. In most methods used previously, the GOI and a selectable marker are expressed from two separate mRNAs, so that their concomitant expression is not guaranteed. In this study, we developed constructs that allow expression of an upstream GOI and downstream selectable marker from a single bicistronic mRNA. Although this approach in other systems has typically required a translation-enhancing element such as an internal ribosome entry site for the downstream marker, we found that a short stretch of unstructured junction sequence was sufficient to obtain adequate expression of the downstream gene, presumably through post-termination reinitiation. With this system, we obtained robust expression of both endogenous and heterologous GOIs, including fluorescent proteins and tagged fusion proteins, in the vast majority of transformants, thus eliminating the need for tedious secondary screening for GOI-expressing transformants. This improved efficiency should greatly facilitate a variety of genetic and cell-biological studies in Chlamydomonas and also enable new applications such as expression-based screens and large-scale production of foreign proteins.
MeSH term(s)	Chlamydomonas reinhardtii/genetics ; Gene Expression ; Gene Order ; Genetic Markers ; Genetic Vectors/genetics ; Promoter Regions, Genetic ; RNA, Messenger/genetics ; Transformation, Genetic ; Transgenes
Chemical Substances	Genetic Markers ; RNA, Messenger
Language	English
Publishing date	2016-12-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	2629978-1
ISSN	2160-1836 ; 2160-1836
ISSN (online)	2160-1836
ISSN	2160-1836
DOI	10.1534/g3.116.033035
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Article ; Online: Analysis of Rho-GTPase Activity During Budding Yeast Cytokinesis.

Onishi, Masayuki / Pringle, John R

Methods in molecular biology (Clifton, N.J.)

2016 Volume 1369, Page(s) 205–218

Abstract: Rho-type small GTPases are involved in cytokinesis in various organisms, but their precise roles and regulation remain unclear. Rho proteins function as molecular switches by cycling between the active GTP-bound and inactive GDP-bound states; the GTP- ... ...

Abstract	Rho-type small GTPases are involved in cytokinesis in various organisms, but their precise roles and regulation remain unclear. Rho proteins function as molecular switches by cycling between the active GTP-bound and inactive GDP-bound states; the GTP-bound proteins in turn interact with their downstream effectors to transmit the signal. Biochemical assays using Rho-binding domains of effector proteins have been used to specifically pull down GTP-bound Rho proteins from cell extracts. Here, we describe the application of such a method in combination with cell-cycle synchronization in the budding yeast Saccharomyces cerevisiae; this approach allows dissection of the activity of Rho1 at different stages of cytokinesis. We also present data showing the importance of caution in interpreting such biochemical data and of comparing to the results obtained with other approaches where possible. The principle of this protocol is also applicable to analyses of other Rho-type GTPases and cell-cycle events.
MeSH term(s)	Cytokinesis ; Protein Binding ; Recombinant Fusion Proteins ; Saccharomycetales/metabolism ; rho GTP-Binding Proteins/metabolism
Chemical Substances	Recombinant Fusion Proteins ; rho GTP-Binding Proteins (EC 3.6.5.2)
Language	English
Publishing date	2016
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-4939-3145-3_15
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Article ; Online: Reduced thermal tolerance in a coral carrying CRISPR-induced mutations in the gene for a heat-shock transcription factor.

Cleves, Phillip A / Tinoco, Amanda I / Bradford, Jacob / Perrin, Dimitri / Bay, Line K / Pringle, John R

Proceedings of the National Academy of Sciences of the United States of America

2020 Volume 117, Issue 46, Page(s) 28899–28905

Abstract: Reef-building corals are keystone species that are threatened by anthropogenic stresses including climate change. To investigate corals' responses to stress and other aspects of their biology, numerous genomic and transcriptomic studies have been ... ...

Abstract	Reef-building corals are keystone species that are threatened by anthropogenic stresses including climate change. To investigate corals' responses to stress and other aspects of their biology, numerous genomic and transcriptomic studies have been performed, generating many hypotheses about the roles of particular genes and molecular pathways. However, it has not generally been possible to test these hypotheses rigorously because of the lack of genetic tools for corals or closely related cnidarians. CRISPR technology seems likely to alleviate this problem. Indeed, we show here that microinjection of single-guide RNA/Cas9 ribonucleoprotein complexes into fertilized eggs of the coral
MeSH term(s)	Animals ; Anthozoa/genetics ; Anthozoa/physiology ; Climate Change ; Clustered Regularly Interspaced Short Palindromic Repeats/genetics ; Computational Biology/methods ; Coral Reefs ; Gene Editing/methods ; Genome/genetics ; Genomics/methods ; Heat Shock Transcription Factors/genetics ; Heat Shock Transcription Factors/metabolism ; Heat-Shock Response/genetics ; Heat-Shock Response/physiology ; Hot Temperature/adverse effects ; Mutation/genetics ; Phenotype ; Temperature ; Transcriptome/genetics
Chemical Substances	Heat Shock Transcription Factors
Language	English
Publishing date	2020-11-09
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	209104-5
ISSN	1091-6490 ; 0027-8424
ISSN (online)	1091-6490
ISSN	0027-8424
DOI	10.1073/pnas.1920779117
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